Comparison of the neural differentiation potential of human mesenchymal stem cells from amniotic fluid and adult bone marrow.


:Human mesenchymal stem cells (MSCs) are considered a promising tool for cell-based therapies of nervous system diseases. Bone marrow (BM) has been the traditional source of MSCs (BM-MSCs). However, there are some limitations for their clinical use, such as the decline in cell number and differentiation potential with age. Recently, amniotic fluid (AF)-derived MSCs (AF-MSCs) have been shown to express embryonic and adult stem cell markers, and can differentiate into cells of all three germ layers. In this study, we isolated AF-MSCs from second-trimester AF by limiting dilution and compared their proliferative capacity, multipotency, neural differentiation ability, and secretion of neurotrophins to those of BM-MSCs. AF-MSCs showed a higher proliferative capacity and more rapidly formed and expanded neurospheres compared to those of BM-MSCs. Both immunocytochemical and quantitative real-time PCR analyses demonstrated that AF-MSCs showed higher expression of neural stemness markers than those of BM-MSCs following neural stem cell (NSC) differentiation. Furthermore, the levels of brain-derived growth factor and nerve growth factor secreted by AF-MSCs in the culture medium were higher than those of BM-MSCs. In addition, AF-MSCs maintained a normal karyotype in long-term cultures after NSC differentiation and were not tumorigenic in vivo. Our findings suggest that AF-MSCs are a promising and safe alternative to BM-MSCs for therapy of nervous system diseases.


Cell Mol Neurobiol


Yan ZJ,Hu YQ,Zhang HT,Zhang P,Xiao ZY,Sun XL,Cai YQ,Hu CC,Xu RX




Has Abstract


2013-05-01 00:00:00












  • BDNF-dependent accumulation of palmitoleic acid in CNS neurons.

    abstract::Although it is known that brain-derived neurotrophic factor (BDNF) plays a critical role in neuronal survival and differentiation, its effect on lipid homeostasis is poorly understood. To understand them, we here investigated the effect of BDNF on the fatty acid composition of primary neurons. A detailed analysis of t...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Suzuki S,Hongli Q,Okada A,Kasama T,Ohta K,Warita K,Tanaka K,Miki T,Takeuchi Y

    更新日期:2012-11-01 00:00:00

  • Myosin IIA Regulated Tight Junction in Oxygen Glucose-Deprived Brain Endothelial Cells Via Activation of TLR4/PI3K/Akt/JNK1/2/14-3-3ε/NF-κB/MMP9 Signal Transduction Pathway.

    abstract::Non-muscle myosin heavy chain IIA (NMMHC IIA), a member of Myosin II family, plays a critical role in various cellular physiological processes. Our previous research had suggested that NMMHC IIA could participate in regulating tight junction morphological changes induced by ischemia stroke. Thus, in the current study,...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Lv Y,Liu W,Ruan Z,Xu Z,Fu L

    更新日期:2019-03-01 00:00:00

  • Exocytosis in single chromaffin cells: regulation by a secretory granule-associated Go protein.

    abstract::1. Besides having a role in signal transduction, trimeric G proteins may also be involved in membrane trafficking events. In chromaffin cells, G alpha o has been found associated with the membrane of secretory granules. Here we examined the role of Go in regulated exocytosis using pressure microinjection combined with...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Vitale N,Gonon F,Thiersé D,Aunis D,Bader MF

    更新日期:1997-02-01 00:00:00

  • PARK2 and PARK7 Gene Polymorphisms as Risk Factors Associated with Serum Element Concentrations and Clinical Symptoms of Parkinson's Disease.

    abstract::Besides clinical and imaging techniques, there is a lack of molecular makers for the diagnosis of Parkinson's disease (PD). There is an immense need to develop biomarkers associated with the phenotypes which may be valuable for individualized treatment. Single-nucleotide polymorphisms (PARK2: Ser167Asn (G>A) and Val38...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Sanyal J,Anirudhan A,Banerjee TK,Guha G,Ramakrishnan Veerabathiran,Murugesan R,Ahmed SSJS,Rao VR

    更新日期:2020-04-01 00:00:00

  • Development of the new group of indole-derived neuroprotective drugs affecting oxidative stress.

    abstract::1. The role of oxidative stress, and accordingly uncontrolled reactive oxygen species generation/action, have been widely documented in a number of different neuronal pathologies. However, the concept of pharmacological interventions in prevention and therapy of oxidative stress-related diseases has not found adequate...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Stolc S,Snirc V,Májeková M,Gáspárová Z,Gajdosíková A,Stvrtina S

    更新日期:2006-10-01 00:00:00

  • Implantation of tumoral XC cells induces chronic, endothelin-dependent, thermal hyperalgesia in mice.

    abstract::1. We describe here the alterations in the nociceptive sensitivity of Swiss CD1 mice receiving an intraplantar ( administration of XC Rous sarcoma-virus-transformed rat fibroblasts (XC cells). 2. Histological studies reveal that XC cells remain at the injection site 2-3 weeks after implantation, a time at which ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Baamonde A,Lastra A,Fresno MF,Llames S,Meana A,Hidalgo A,Menéndez L

    更新日期:2004-04-01 00:00:00

  • Glial cells are involved in the exciting effects of doxapram on brainstem slices in vitro.

    abstract::This study tested whether the glial cells are involved in the exciting effects of doxapram on brainstem slice in vitro. Experiments were performed in brainstem slice preparations from neonatal rats. The medial area of nucleus retrofacialis (mNRF) and the hypoglossal nerve (XII nerve) were contained in the preparations...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Li GC,Zhang HT,Jiao YG,Wu ZH,Fang F,Cheng J

    更新日期:2010-07-01 00:00:00

  • Pre-clinical analysis of changes in intra-cellular biochemistry of glioblastoma multiforme (GBM) cells due to c-Myc silencing.

    abstract::Glioblastoma Multiforme (GBM) is an aggressive form of brain Tumor that has few cures. In this study, we analyze the anti-proliferative effects of a new molecule JQ1 against GBMs induced in Wistar Rats. JQ1 is essentially a Myc inhibitor. c-Myc is also known for altering the biochemistry of a tumor cell. Therefore, th...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Rajagopalan V,Vaidyanathan M,Janardhanam VA,Bradner JE

    更新日期:2014-10-01 00:00:00

  • Evidence for the production and action of interleukin-10 in pituitary cells.

    abstract::1. Interleukin-10 (IL-10) has a wide range of activities in the immune system such as modulation of interferon-gamma (IFN-gamma) and antibody production. The neuropeptide hormone corticotropin (ACTH) has similar activities, suggesting that a bidirectional communication mechanism operates between the immune and the neu...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Hughes TK,Cadet P,Rady PL,Tyring SK,Chin R,Smith EM

    更新日期:1994-02-01 00:00:00

  • Combined actions of Pb2+, Zn2+, and Al3+ on voltage-activated calcium channel currents.

    abstract::1. Whole-cell patch clamp experiments were performed on rat dorsal root ganglion (DRG) neurons to investigate the actions of various combinations of Pb2+, Zn2+, and Al3+ on voltage-activated calcium channel currents (VACCCs). 2. Each of these metals has been shown to reduce VACCCs. 3. We investigated the effects of si...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Platt B,Büsselberg D

    更新日期:1994-12-01 00:00:00

  • Effect of ventral noradrenergic bundle lesions on concentrations of monoamine neurotransmitters and metabolites in several discrete areas of the rat brain.

    abstract::1. The effects of radiofrequency lesions of the ventral noradrenergic bundle (VNB) on monoamine and metabolite concentrations in several discrete areas of the rat hypothalamus were examined. Monoamines and metabolites were analyzed utilizing high-performance liquid chromatography coupled with electrochemical detection...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Johnston CA,Mattiace LA,Negro-Vilar A

    更新日期:1987-12-01 00:00:00

  • Age-Dependency of Levetiracetam Effects on Exocytotic GABA Release from Nerve Terminals in the Hippocampus and Cortex in Norm and After Perinatal Hypoxia.

    abstract::Perinatal hypoxia can lead to multiple chronic neurological deficits, e.g., mental retardation, behavioral abnormalities, and epilepsy. Levetiracetam (LEV), 2S-(2-oxo-1-pyrrolidiny1) butanamide, is an anticonvulsant drug with proven efficiency in treating patients with focal and generalized seizures. Rats were underwe...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Pozdnyakova N,Dudarenko M,Borisova T

    更新日期:2019-07-01 00:00:00

  • Down-regulated GABAergic expression in the olfactory bulb layers of the mouse deficient in monoamine oxidase B and administered with amphetamine.

    abstract::This study explores primarily the role of the activity of monoamine oxidase B (MAOB) in the regulation of glutamic acid decarboxylase(67) (GAD(67)) expression in distinct layers of main olfactory bulb (OlfB), which links the limbic system. Moreover, the response of GAD(67) was investigated to amphetamine perturbation ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Yin HS,Chen K,Shih JC,Tien TW

    更新日期:2010-05-01 00:00:00

  • Hck Promotes Neuronal Apoptosis Following Intracerebral Hemorrhage.

    abstract::The hematopoietic cell kinase (Hck) is a member of the Src family protein kinases which regulates many signal transduction pathways including cell growth, proliferation, differentiation, migration, and apoptosis. However, the expression and function of Hck after intracerebral hemorrhage (ICH) are unknown. Western blot...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Wang J,Chen R,Liu X,Shen J,Yan Y,Gao Y,Tao T,Shi J

    更新日期:2017-03-01 00:00:00

  • Differential responses of rat pineal thyroxine type II 5'-deiodinase and N-acetyltransferase activities to either light exposure, isoproterenol, phenylephrine, or propranolol.

    abstract::1. Compared to pineal N-acetyl transferase (NAT) activity, which exhibited a dramatic drop following acute light exposure at night, nocturnal rat pineal thyroxine type II 5'-deiodinase (5'-D) activity was minimally influenced by the same light exposure. The injection of cycloheximide, a potent inhibitor of protein syn...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Guerrero JM,Puig-Domingo M,Santana C,Menendez-Pelaez A,Gonzalez-Brito A,Reiter RJ

    更新日期:1988-12-01 00:00:00

  • Pharmacodynamic study of FS-0311: a novel highly potent, selective acetylcholinesterase inhibitor.

    abstract::(1) This study was to evaluate the anti-cholinesterase (ChE), cognition enhancing and neuroprotective effects of FS-0311, a bis-huperzine B derivative. (2) ChE activity was evaluated using a spectrophotometric method. Cognitive deficits in mice were induced by scopolamine or transient brain ischemia and reperfusion. W...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Wang ZF,Yan J,Fu Y,Tang XC,Feng S,He XC,Bai DL

    更新日期:2008-02-01 00:00:00

  • RLIP76 Depletion Enhances Autophagic Flux in U251 Cells.

    abstract::Our previous study showed that RalA-binding protein 1 (RLIP76) is overexpressed in gliomas and is associated with higher tumour grade and decreased patient survival. Furthermore, RLIP76 downregulation increases chemosensitivity of glioma cells to temozolomide by inducing apoptosis. However, other mechanisms underlying...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Zhang C,Cai Z,Liang Q,Wang Q,Lu Y,Hu L,Hu G

    更新日期:2017-04-01 00:00:00

  • Guggulipid and nimesulide differentially regulated inflammatory genes mRNA expressions via inhibition of NF-kB and CHOP activation in LPS-stimulated rat astrocytoma cells, C6.

    abstract::Neuroinflammation is an integral part of neurodegenerative diseases. Lipo-polysacharide (LPS) induces reactive astrogliosis, the cellular manifestation of neuroinflammation, in various models of neurological diseases, but its mechanism of action is still not properly known. The effect of guggulipid and nimesulide on L...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Niranjan R,Nath C,Shukla R

    更新日期:2011-07-01 00:00:00

  • Serine Racemase Expression by Striatal Neurons.

    abstract::D-serine is synthesized by serine racemase (SR) and is a co-agonist at forebrain N-methyl-D-aspartate receptors (NMDARs). D-serine and SR are expressed primarily in neurons, but not in quiescent astrocytes. In this study, we examined the localization of D-serine and SR in the mouse striatum and the effects of genetica...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Takagi S,Puhl MD,Anderson T,Balu DT,Coyle JT

    更新日期:2020-05-22 00:00:00

  • Function of Cathepsin K in the Central Nervous System of Male Mice is Independent of Its Role in the Thyroid Gland.

    abstract::Cathepsin K deficiency in male mice (Ctsk-/-) results in decreased numbers of hippocampal astrocytes and altered neuronal patterning as well as learning and memory deficits. Additionally, cathepsin K carries essential roles in the thyroid gland where it contributes to the liberation of thyroid hormones (TH). Because T...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Dauth S,Rakov H,Sîrbulescu RF,Ilieş I,Weber J,Batbajar Dugershaw B,Braun D,Rehders M,Wirth EK,Führer D,Schweizer U,Brix K

    更新日期:2020-07-01 00:00:00

  • Cell proliferation in the adult rat rostral migratory stream following exposure to gamma irradiation.

    abstract::One of the few areas of the adult CNS, that are known to be competent for neuronal proliferation, is the subventricular zone (SVZ) lining the brain lateral ventricles. Cells proliferating in the SVZ migrate along a defined pathway, the rostral migratory stream (RMS), where their proliferation continues until reaching ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Bálentová S,Raceková E,Martoncíková M,Misúrová E

    更新日期:2006-10-01 00:00:00

  • Intrathecal adenosine A1 receptor agonist attenuates hyperalgesia without inhibiting spinal glutamate release in the rat.

    abstract::The analgesia effects of intrathecal adenosine A1 receptor agonist, R-PIA, on the hyperalgesia and CSF-glutamate release after formalin injection into the rat paw were evaluated. R-PIA significantly and dose-dependently attenuated increases in flinching behavior, and this attenuating effect was reversed by the adenosi...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Yamamoto S,Nakanishi O,Matsui T,Shinohara N,Kinoshita H,Lambert C,Ishikawa T

    更新日期:2003-04-01 00:00:00

  • Standardised extract of Bacopa monniera (CDRI-08) improves contextual fear memory by differentially regulating the activity of histone acetylation and protein phosphatases (PP1α, PP2A) in hippocampus.

    abstract::Contextual fear conditioning is a paradigm for investigating cellular mechanisms involved in hippocampus-dependent memory. Earlier, we showed that standardised extract of Bacopa monniera (CDRI-08) improves hippocampus-dependent learning in postnatal rats by elevating the level of serotonin (5-hydroxytryptamine, 5-HT),...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Preethi J,Singh HK,Venkataraman JS,Rajan KE

    更新日期:2014-05-01 00:00:00

  • Alterations of glial cells in the mouse hippocampus during postnatal development.

    abstract::We investigated the postnatal alterations of neurons, astrocyte, oligodendrocyte, and microglia in the mouse hippocampal CA1 sector and dentate gyrus under the same conditions using immunohistochemistry. Neuronal nuclei (NeuN), Glial fibrillary acidic protein (GFAP), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPas...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Kimoto H,Eto R,Abe M,Kato H,Araki T

    更新日期:2009-12-01 00:00:00

  • Retinal dopamine D1 and D2 receptors: characterization by binding or pharmacological studies and physiological functions.

    abstract::1. In the retinal inner nuclear layer of the majority of species, a dopaminergic neuronal network has been visualized in either amacrine cells or the so-called interplexiform cells. 2. Binding studies of retinal dopamine receptors have revealed the existence of both D1- as well D2-subtypes. The D1-subtype was characte...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,评审


    authors: Schorderet M,Nowak JZ

    更新日期:1990-09-01 00:00:00

  • Up-regulation of podoplanin involves in neuronal apoptosis in LPS-induced neuroinflammation.

    abstract::Podoplanin (PDPN) is a mucin-type transmembrane sialoglycoprotein expressed in multiple tissues in adult animals, including the brain, lungs, kidney, and lymphoid organs. Studies of this molecule have demonstrated its great importance in tumor metastasis, platelet aggregation, and lymphatic vessel formation. However, ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Song Y,Shen J,Lin Y,Shen J,Wu X,Yan Y,Zhou L,Zhang H,Zhou Y,Cao M,Liu Y

    更新日期:2014-08-01 00:00:00

  • Spermidine Exhibits Protective Effects Against Traumatic Brain Injury.

    abstract::Traumatic brain injury (TBI) causes permanent neurological and cognitive impairments. Effective pharmacological interventions remain elusive. Spermidine is a polyamine compound found in our body that may play a role in brain development and congenital function. In this study, we aimed to investigate the therapeutic po...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Huang J,Zhang H,Zhang J,Yu H,Lin Z,Cai Y

    更新日期:2020-08-01 00:00:00

  • Global Histone H4 Acetylation in the Olfactory Bulb of Lactating Rats with Different Patterns of Maternal Behavior.

    abstract::In rats, variations in the levels of neuromodulatory molecules and in the expression of their receptors are observed during pregnancy and postpartum. These changes may contribute to the development and management of maternal behavior. The frequency of licking the pups is used to evaluate maternal care, having mothers ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: de Moura AC,da Silva IR,Reinaldo G,Dani C,Elsner VR,Giovenardi M

    更新日期:2016-10-01 00:00:00

  • Involvement of nuclear factor-kappaB in endothelin-A-receptor-induced proliferation and inhibition of apoptosis.

    abstract::Endothelins have been implicated in the regulation of cell proliferation, differentiation, and apoptosis, but the mechanisms of these complex events are not yet fully understood. Although the nuclear factor-kappaB (NF-kappaB) was shown to play a prominent role in the above processes, its participation in endothelin re...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Mangelus M,Galron R,Naor Z,Sokolovsky M

    更新日期:2001-12-01 00:00:00

  • Cytidine and uridine increase striatal CDP-choline levels without decreasing acetylcholine synthesis or release.

    abstract:AIMS:Treatments that increase acetylcholine release from brain slices decrease the synthesis of phosphatidylcholine by, and its levels in, the slices. We examined whether adding cytidine or uridine to the slice medium, which increases the utilization of choline to form phospholipids, also decreases acetylcholine levels...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Ulus IH,Watkins CJ,Cansev M,Wurtman RJ

    更新日期:2006-07-01 00:00:00