Abstract:
:Osteoprogenitors present in cell populations derived from fetal or newborn rat and mouse calvaria differentiate in long term culture and form osteoblastic bone-forming colonies (bone nodules). Previous reports have indicated considerable differences between bone cell populations derived from these two species with regard to their proliferation in response to glucocorticoids. In the present investigation, we have focused on proliferation and differentiation of osteoprogenitor cells in these bone cell populations and evaluated the effect of corticosterone, the principal glucocorticoid of both mouse and rat. Cells were isolated by sequential collagenase digestion from calvaria of newborn (2-5 days) CD-1 mice [mouse calvariae (MC) cells] and term fetal Wistar rats [rat calvaria (RC) cells] and cultured for up to 25 days in alpha-minimal essential medium containing 10% fetal bovine serum (FBS), antibiotics, 50 microg/mL ascorbic acid, and 8-10 mmol/L beta-glycerophosphate. In agreement with previous observations by us and others, corticosterone increased cell growth in RC cell cultures, but inhibited cell growth in MC cultures. In RC cell cultures, corticosterone (1-1000 nmol/L) increased the nodule number in a dose-dependent manner (p < 0.001 for all concentrations above 3 nmol/L) with a maximal effect at 300 and 1000 nmol/L (threefold increase over control). In MC cells, on the other hand, corticosterone (0.3-1000 nmol/L) increased the nodule number only at 30 nmol/L (50%, p < 0.01) but inhibited nodule formation by 33% (p < 0.001) at 1000 nmol/L. In both RC and MC cultures a linear relationship was found between the number of cells plated and number of nodules formed. When cultures were treated with cortisol (30-300 nmol/L), similar effects were observed; the number of nodules dose dependently increased in RC cell cultures and dose dependently decreased in MC cell cultures. Significantly, however, the inactive glucocorticoid cortisone also increased bone nodule formation in RC cell cultures and decreased bone nodule formation in MC cell cultures. Carbenoxolone, which blocks 11 beta hydroxysteroid dehydrogenase and thus prevents conversion of cortisone to cortisol, partially inhibited the cortisone-induced effects on bone nodule formation in both RC and MC cell cultures, indicating that both RC and MC cells can convert inactive glucocorticoids to active metabolites. In conclusion, our results show that the glucocorticoids corticosterone and cortisol inhibit proliferation and differentiation of osteoprogenitors in MC cell cultures but stimulate these processes in rat-derived osteoprogenitors.
journal_name
Bonejournal_title
Boneauthors
Bellows CG,Ciaccia A,Heersche JNdoi
10.1016/s8756-3282(98)00084-2subject
Has Abstractpub_date
1998-08-01 00:00:00pages
119-25issue
2eissn
8756-3282issn
1873-2763pii
S8756328298000842journal_volume
23pub_type
杂志文章相关文献
BONE文献大全abstract::The aim of the study was to assess the biomechanical effects of short-term PTH treatment and withdrawal on bone mass and strength in an aged, osteopenic, ovariectomized (ovx) rat model. Additionally, the effect of sequential therapy with PTH and the bisphosphonate, risedronate, the effect of longterm PTH monotherapy, ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(97)00024-0
更新日期:1997-05-01 00:00:00
abstract::Although ovariectomized cynomolgus monkeys are used extensively for studies examining perimenopausal changes in the skeleton, very little is known about the changes that occur naturally during growth and aging in these animals. To evaluate the changes in bone during growth and aging in female cynomolgus monkeys, 29 mo...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(98)00149-5
更新日期:1999-01-01 00:00:00
abstract::Avian tibial dyschondroplasia is a disorder of endochondral ossification which results in the accumulation of noncalcified, avascular cartilage. We have investigated the changes in cell proliferation and enzyme activities within specific cell types in the growth plate of chickens with differing severity of the disease...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(92)90362-z
更新日期:1992-01-01 00:00:00
abstract:OBJECTIVE:To establish a drill-hole defect model in osteoporotic mouse femur by comparing temporal cortical bone healing pattern between OVX-induced osteoporotic bone and sham-operated bone. METHODS:3-month-old female C57BL/6 mice were randomly divided into an ovariectomy group (OVX) and a sham-operated group (Sham). ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.03.720
更新日期:2011-06-01 00:00:00
abstract::Several animal studies have showed that gelatin may be effective for minimizing bone loss in OVX rats with established osteopenia. To gain insight into how cod bone gelatin administration affects bone loss after ovariectomy, studies were carried out focusing on bone quality and the molecular mechanisms. Eighty-four fe...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2008.12.005
更新日期:2009-05-01 00:00:00
abstract::Pre-clinical in vivo models of multiple myeloma are essential tools for investigating the pathophysiology of multiple myeloma and for testing new therapeutic agents and strategies prior to their potential use in clinical trials. Over the last five decades, several different types of murine models of multiple myeloma h...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2015.04.004
更新日期:2015-08-01 00:00:00
abstract::Leptin has been suggested to mediate a variety of actions, including bone development, via its ubiquitously expressed receptor (Ob-Rb). In this study, we investigated the role of leptin in endochondral ossification at the growth plate. The growth plates of wild-type and ob/ob mice were analyzed. Effects of leptin on c...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.05.009
更新日期:2005-11-01 00:00:00
abstract::Bone mineral density (BMD) is influenced by both environmental and genetic factors. We previously reported the association of the RUNX2 A allele with increased bone mineral density (BMD) and protection against a common form of osteoporotic fracture within a Geelong population. We genotyped 991 women from a Scottish co...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.02.004
更新日期:2004-06-01 00:00:00
abstract::The purpose of our study was to investigate the recovery of bone disease in celiac patients during 5 years of a gluten-free diet. The study group consisted of 28 newly diagnosed celiac patients (9 men, 19 women) recruited between 1990 and 1991. Six patients withdrew from the 5-year follow-up. Compliance with the glute...
journal_title:Bone
pub_type: 临床试验,杂志文章
doi:10.1016/s8756-3282(99)00171-4
更新日期:1999-09-01 00:00:00
abstract::Bone healing is a complex multi-step process, which depends on the position and size of the lesion, and on the mechanical stability of the wounded area. To address more specifically the mechanisms involved in cortical bone healing, we created drill-hole defects in the cortex of mouse femur, a lesion that triggers intr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.09.007
更新日期:2010-02-01 00:00:00
abstract:OBJECTIVES:Osteoarthritis (OA) is a disease of the whole joint characterized by cartilage loss and subchondral bone remodeling. The role of microcracks in cartilage integrity and subchondral bone homeostasis is not fully understood. The main goal of this work was to evaluate microcrack density in both calcified cartila...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.03.011
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:Fibrodysplasia ossificans progressiva (FOP) is a catastrophic genetic disorder of progressive heterotopic ossification (HO). Assessment of functional mobility in FOP will be essential to support clinical trials of investigational agents. RESULTS:Of necessity, we developed a simple, rapidly-administered, cum...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.04.015
更新日期:2017-08-01 00:00:00
abstract::Bisphosphonates (BPs), bone targeted drugs that disrupt osteoclast function, are routinely used to treat complications of bone metastasis. Studies in preclinical models of cancer have shown that BPs reduce skeletal tumor burden and increase survival. Similarly, we observed in the present study that administration of t...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.01.010
更新日期:2009-05-01 00:00:00
abstract::The present work was designed to investigate the effects of oscillating fluid flow on gap junctional intercellular communication (GJIC) and the gap junction protein connexin (Cx) 43 in osteocyte-like MLOY-4 cells. Cells were exposed for 1 h to oscillating fluid flow at a shear stress of +/-10 dyn/cm(2) and a frequency...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00167-4
更新日期:2003-07-01 00:00:00
abstract::Cartilage canals are temporary vessel-containing structures within the growth cartilage. The canals gradually regress with age in a process designated chondrification, where the content of the canals is replaced by cartilage. The process of chondrification is considered physiological; however, premature regression has...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.08.016
更新日期:2004-12-01 00:00:00
abstract::In some types of low-trauma fractures of older adults the number and incidence of fractures are stabilizing or even decreasing. However, very little population-based information is available concerning the nationwide numbers, incidences and especially secular trends of low-trauma fractures of the distal humerus. There...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.11.025
更新日期:2010-05-01 00:00:00
abstract::SOST encodes sclerostin, an inhibitor of bone formation that antagonizes canonical Wnt signaling. Variations of SOST expression have an impact on bone mineral density (BMD) and bone strength. We hypothesized that genetic and epigenetic DNA modifications have an impact on SOST gene expression. By analyzing 43 bone samp...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.08.004
更新日期:2016-11-01 00:00:00
abstract::The responses of bone cells to skeletal loading are clearly an important factor in bone biology, but much remains to be learned about the role of these responses in skeletal development, maintenance, and tissue repair. Bone morphogenetic proteins (BMPs) are key regulators of bone formation. We examined the effect of B...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(02)00865-7
更新日期:2002-11-01 00:00:00
abstract:BACKGROUND:Vertebral fractures (VF) upon Denosumab (DMAB) discontinuation were first described as a distinct phenomenon in 2015, yet the magnitude of this event remains undetermined. OBJECTIVES:To estimate fracture risk after DMAB discontinuation, in a real-world setting. METHODS:The computerized database of a 2.3-mi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115150
更新日期:2020-01-01 00:00:00
abstract::The process of bone turnover displays variations over 24 h, with C-terminal cross-linked telopeptide of type 1 collagen (CTX) and osteocalcin exhibiting a nadir in the afternoon and a peak in the night. In contrast, N-terminal propeptide of type 1 procollagen (P1NP) did not display an apparent 24-hour rhythm. Other em...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.10.002
更新日期:2019-03-01 00:00:00
abstract::Thanatophoric dysplasia (TD) is a lethal human bone dysplasia characterized by severe dwarfism. It pathogenesis is thought to involve an abnormal ossifying fibrous tissue that disrupts the skeletal growth plate. We employed a combined morphologic, immunohistochemical and biochemical approach to better define the natur...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(88)90027-0
更新日期:1988-01-01 00:00:00
abstract::While the role of T cells in the regulation of bone homeostasis is well defined, little is known about the role of innate lymphoid cells (ILCs) on bone. ILCs are innate immune cells that share cytokine expression patterns with T cells but lack the T cell receptor. In this study we show that type 2 ILCs (ILC2) potently...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115335
更新日期:2020-07-01 00:00:00
abstract::It was the aim of this retrospective analysis to examine the influence of low-dose monophasic oral contraceptives (OCs) on bone mineral density (BMD) of the femoral neck and of the spine in young female endurance athletes. Data on training intensity, dietary intake, menarche, menstrual cycle disorders, years of OC use...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.05.017
更新日期:2004-10-01 00:00:00
abstract::Matrix vesicles (MVs) are membrane organelles found in the extracellular matrix of calcifying cells, which contain matrix processing enzymes and regulate the extracellular environment via action of these enzymes. It is unknown whether MVs are also exosomic mediators of cell-cell communication via transfer of RNA mater...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.03.018
更新日期:2016-07-01 00:00:00
abstract::Sulfate conjugation is an important pathway in the metabolism of many drugs, xenobiotic compounds, and hormones. Sulfotransferases (SULTs) catalyze these reactions and have been detected and characterized in various human tissues including the liver and small intestine. Substrates for SULTs that include estrogen and t...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00463-x
更新日期:2001-06-01 00:00:00
abstract::Bone can be viewed as a nano-fibrous composite with complex hierarchical structures. Its deformation and fracture behaviors depend on both the local structure and the type of stress applied. In contrast to the extensive studies on bone fracture under compression and tension, there is a lack of knowledge on the fractur...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.10.001
更新日期:2015-02-01 00:00:00
abstract::Osteoporosis is the consequence of altered bone metabolism resulting in the systemic reduction of bone strength and increased risk of fragility fractures. MicroRNAs (miRNAs) regulate gene expression on a post-transcriptional level and are known to take part in the control of bone formation and bone resorption. In addi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.05.027
更新日期:2015-10-01 00:00:00
abstract::Bone morphogenetic protein 2 (BMP-2) is a secreted signaling molecule that acts as an inducer of bone formation and a regulator of embryonic development. The objectives of this work were as follows: (1) to clone the full-length cDNA of BMP-2 in a marine fish model, (2) analyze its gene expression during development, i...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.06.021
更新日期:2006-12-01 00:00:00
abstract::Osteoporosis and fracture risk are common complex diseases, caused by an interaction of numerous disease susceptibility genes and environmental factors. With the advances in genomic technologies, large-scale genome-wide association studies (GWAS) have been performed which have broadened our understanding of the geneti...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2019.04.005
更新日期:2019-09-01 00:00:00
abstract::Female Wistar rats aged 3 months were ovariectomized (OX, n = 27). Three months later they were given i.v. doses of 150 (6), 300 (7), or 600 (6) ug/kg 2/wk of olpadronate during 12 weeks or left as OX controls (OXc). Bending fracture load of femur diaphyses, reduced in OXc, was recovered by olpadronate. This effect wa...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(95)00314-4
更新日期:1995-10-01 00:00:00