Abstract:
PURPOSE:Herceptin is widely used in treating Her2-overexpressing breast cancer. However, the application of Herceptin in prostate cancer is still controversial. Our previous results have indicated the relevance of Her2 in the transition of the androgen requirement in prostate cancer cells. In this study, the effects of radioimmunotherapy against Her2 in prostate cancer were investigated. MATERIALS AND METHODS:DU145, an androgen receptor-negative prostate cancer cell line, was used in vitro and in vivo to evaluate the effects of Herceptin labeled with a beta emitter, Rhenium-188 (Re-188). Its effects on cell growth, extent of apoptosis, the bio-distribution of Re-188 labeled Herceptin (Re-H), and protein levels were determined. RESULTS:Treatments with Re-188 and Re-H reduced the proliferation of DU145 cells in dose- and time-dependent manners compared to the Herceptin-treated group. Growth inhibition and apoptosis were induced after Re-H treatment; growth inhibition was more distinct in cells with high Her2/p-Her2 levels. Our in vivo xenograft studies revealed that Re-H treatment significantly retarded tumor growth and altered the levels of apoptosis-related proteins. The bio-distribution of Re-H in mice demonstrated a tissue-specific pattern. Importantly, the levels of p35 protein, which is related to cancer cell survival and invasion, dramatically decreased after Re-H treatment. CONCLUSIONS:Our data demonstrate that Re-188-labeled Herceptin effectively inhibited the growth of DU145 cells compared to the Herceptin- and Re-188-treated cohorts. This implies that targeting Her2 by both radio- and immuno- therapy might be a potential strategy for treating patients with androgen-independent prostate cancer.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Wang HY,Lin WY,Chen MC,Lin T,Chao CH,Hsu FN,Lin E,Huang CY,Luo TY,Lin Hdoi
10.3109/09553002.2013.762136subject
Has Abstractpub_date
2013-05-01 00:00:00pages
346-55issue
5eissn
0955-3002issn
1362-3095journal_volume
89pub_type
杂志文章abstract::The existence of a correlation between experimental probabilities of cell inactivation by charged particles and calculated probabilities for K-vacancy production in heavy atoms (C, N, O, P) of the DNA of cell nuclei is established. Both phenomena display a similar dependence upon the linear energy transfer (LET) of in...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550601
更新日期:1994-05-01 00:00:00
abstract:PURPOSE:The molecular basis of gene regulation in cells exposed to ionising radiation is not fully understood. Gene regulation occurs at the transcriptional and post-transcriptional levels. Recent studies have suggested that micro-RNA (miRNA) plays a significant role at the post-transcriptional gene regulation. miRNA a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553001003734568
更新日期:2010-07-01 00:00:00
abstract::The effects of UV radiation on humans and animals are receiving increasing attention and much interest has recently been focused on the environmental effects of UV A and UV B. This study compares the in vitro effects of UV A and UV B on the clonogenic survival of two human skin keratinocyte cell lines, HaCaT which are...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143590
更新日期:1997-07-01 00:00:00
abstract::Purpose: Ionizing radiation is a risk factor to the whole organism, including the heart. Cardiac damage is considered to be a late effect of radiation exposure. While the acute cardiotoxicity of high doses is well characterized, the knowledge about nature and magnitude of the cardiac risk following lower doses exposur...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2020.1704299
更新日期:2020-03-01 00:00:00
abstract::During previous studies on the relationship between thermosensitivity and intracellular pH (pHi) regulation in mammalian cells, we observed that the thermoresistant TR-4 cells appeared to be resistant to manipulations of the Na+/H+ antiport, one of the two primary membrane regulators of pHi. We hypothesized that this ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096144969
更新日期:1996-10-01 00:00:00
abstract::Only a small percentage of the DNA damage set by ionizing radiation in the living cell manifests itself as lethal. It is now increasingly accepted that clustered lesions may constitute the kind of damage that the repair enzymes cannot adequately deal with. The question is raised as to whether damage amplification reac...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414551501
更新日期:1994-11-01 00:00:00
abstract::Four of the most radiosensitive xrs variants of CHO-K1 cells, obtained after mutagenizing treatment with EMS, have been studied in detail over three to five decades of cell survival. Although these lines were initially reported to have very steep exponential survival curves, and to vary in sensitivity between themselv...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914550651
更新日期:1989-04-01 00:00:00
abstract:PURPOSE:The main aim of the present study is to gain mechanistic insights into the modulating effect of molecular hydrogen on the γ-radiation-induced alteration pathways of DNA nucleobases. MATERIALS AND METHODS:Aerated aqueous solutions of calf thymus DNA were exposed to a (60)Co source at doses ranging from 0 to 55 ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2016.1206234
更新日期:2016-09-01 00:00:00
abstract::The mechanisms of radiation damage production are used to examine the following premises: (1) the number of DNA double-strand breaks per unit dose increases with dose; (2) cell type to cell type variations in yield of DNA dsb per dose occur. Two stages of damage production are identified as possible sources of damage ...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009014551251
更新日期:1990-06-01 00:00:00
abstract::One of the important temporal stages of radiation action in cellular systems is the chemical phase, where oxygen fixation reactions compete with chemical repair reactions involving reducing agents such as GSH. Using the gas explosion technique it is possible to follow the kinetics of these fast (greater than 1 ms) rea...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214551571
更新日期:1992-06-01 00:00:00
abstract::Free radical formation in aqueous solutions of ubiquinone (coenzyme Qo) induced by ultrasound was studied by EPR and spin trapping with 3,5-dibromo-4-nitrosobenzene sulphonate. When aqueous solutions of ubiquinone were sonicated with 50-kHz ultrasound in the presence of argon or nitrogen, radicals formed by addition o...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096146246
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:The effect of boronophenylalanine (BPA)-mediated boron neutron capture therapy (BNCT) on human oral squamous cell carcinoma (SCC) xenografts in nude mice was examined. MATERIALS AND METHODS:Tumor-bearing mice were given BPA at a dose of 250 mg/kg body weight. The tumor (10)B concentration 2 h after an injectio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000600570453
更新日期:2006-01-01 00:00:00
abstract::SR 2508 (etanidazole) (1 mM) or buthionine sulphoximine (BSO, 50 microM) or both drugs together did not radiosensitize oxic V79 Chinese hamster cells irradiated at either an acute dose rate (2.35 Gy/min) or at a low dose rate (0.117 Gy/min). BSO pretreatment (15 h at 37 degrees C) depleted cellular glutathione (GSH) t...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550121
更新日期:1991-01-01 00:00:00
abstract:PURPOSE:Caveolin-1 is a membrane protein highly expressed in many tumors and plays an important role in tumor progression and metastasis. This review describes the structure of the Caveolin-1 protein and its pre-clinical and clinical significance, demonstrating that Caveolin-1 is a novel biomarker for radioresistance w...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2016.1222096
更新日期:2016-12-01 00:00:00
abstract:PURPOSE:We computed probabilities to have multiple double-strand breaks (DSB), which are produced in DNA on a regional scale, and not in close vicinity, in volumes matching the size of DNA damage foci, of a large chromatin loop, and in the physical volume of DNA containing the HPRT (human hypoxanthine phosphoribosyltra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802499212
更新日期:2008-11-01 00:00:00
abstract:BACKGROUND:Element-specific biokinetic models are used to reconstruct doses to systemic tissues from internal emitters. Typically, a systemic model for a radionuclide explicitly depicts only its dominant repositories. Remaining tissues and fluids are aggregated into a pool called Other tissue in which the radionuclide ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2018.1554923
更新日期:2018-12-04 00:00:00
abstract::Genetic analysis was performed on 72 Italian A-T families ascertained through 91 probands. The frequency of the A-T gene was estimated through Dahlberg's formula, which uses the frequencies of first cousin marriages among the patients' parents and in the general population were obtained from the collection of Catholic...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract:PURPOSE:Melanoma is mainly treated by surgery and rarely with radiation because of the high radioresistance of this tumor. Nevertheless, radiotherapy is the preferred treatment modality for unresectable lesions and avoiding cosmetic disfigurement caused by surgical excision. This study investigated the therapeutic adva...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2017.1276309
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:Chronic occupational exposure to ionizing radiation (IR) induces a wide spectrum of DNA damages. The aim of this study was to assess the frequencies of micronucleus (MN), sister chromatid exchanges (SCE) and to evaluate their association with XRCC1 399 Arg/Gln and XRCC3 241 Thr/Met polymorphisms in Hospital sta...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2017.1377361
更新日期:2017-11-01 00:00:00
abstract:PURPOSE:To construct a quantitative model of the radiation-induced bystander effect based on diffusion-type spreading of bystander signal communication between the hit and non-hit cells. Cell inactivation and induced oncogenic transformation by broad- and microbeam irradiation systems are considered. MATERIALS AND MET...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::High doses of rHIL-1 protected C3H/HeN mice against lethal radiation damage. The optimum time of radioprotection was found when this cytokine was administered at 4 h and at 20 h before irradiation. The dose reduction factors when the IL-1 was administered at those times were about 1.30. The radioprotection at the opti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914551621
更新日期:1989-10-01 00:00:00
abstract::This study simulates the exposure of V79 Chinese hamster fibroblasts to low-energy protons, deuterons and alpha-particles in the LET range 10-200 keV/microm. The starting assumption is that the induction of clustered lesions in DNA is a fundamental step for cell inactivation. A non-homogeneous cell population was simu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143004
更新日期:1997-11-01 00:00:00
abstract::A study has been made of the intrinsic radiosensitivity of peripheral blood lymphocytes from normal individuals. Cell survival following in vitro high (1.55 Gy min-1) and low (0.0098 Gy min-1) dose-rate irradiation was obtained for single lymphocyte samples from three individuals in six experiments. Despite wide inter...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551571
更新日期:1993-10-01 00:00:00
abstract:PURPOSE:To study the oxidative stress-induced changes by sublethal doses of cadmium chloride (CdCl2) and gamma irradiation, two redox-inducing agents, on metallothionein (MT) gene and protein expression in Plantago ovata Forsk (P. ovata). MATERIALS AND METHODS:Chlorophyll content was estimated to study the stress resp...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.782109
更新日期:2013-07-01 00:00:00
abstract:PURPOSE:The chromosomal radiosensitivity of a selected group of familial breast cancer patients carrying a mutation in BRCA1 (n=11) or BRCA2 (n=9) and a group of healthy mutation carriers (n=12) was investigated and compared to a reference group of breast cancer patients without a BRCA1/2 mutation (n=78) and a group of...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000400017937
更新日期:2004-10-01 00:00:00
abstract::Induction by gamma-radiation, UV radiation or hydroxyurea of RecA gene product synthesis in Escherichia coli, monitored as beta-D-galactosidase in recA-lacZ fusion strains, was shown to be inhibited if 2-mercaptoethylamine (MEA) was added before treatment with the inducing agents. If cysteine (Cys) at low concentratio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096146228
更新日期:1996-01-01 00:00:00
abstract::We used three common fluorescent probes to measure pericentric inversion frequencies in 2.9 Gy 60Co gamma-irradiated human lymphocytes. For a given chromosome, the first probe is specific to one telomeric region, the second probe is specific to one subcentromeric region and the third probe is specific to the centromer...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097144382
更新日期:1997-01-01 00:00:00
abstract::The effect of a siderophore analogue 3,4,3-LIHOPO has been investigated in rat after intramuscular injection of 238Pu, 239Pu and 241Am simulating puncture wounds. Various treatment regimens were used to remove the radioactivity from its injection site and to reduce its retention in body tissues. The local deposits cou...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145382
更新日期:1996-07-01 00:00:00
abstract:PURPOSE:This study is part of a general search for the anatomical bases of the severe mental retardation syndrome caused by prenatal irradiation. More specifically, it seeks reasons for the high radiosensitivity of a white matter area, the cingulum of the corpus callosum. MATERIALS AND METHODS:Pregnant primiparous Wis...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139494
更新日期:1999-10-01 00:00:00
abstract::Terminally differentiated cells usually do not divide and are, thus, reproductively dead. To elucidate the significance of radiation-enhanced differentiation to reproductive cell death, murine erythroid progenitor cells were gamma-irradiated in plasma clot cultures and the development of haemoglobinized clones was stu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145869
更新日期:1996-03-01 00:00:00