Abstract:
PURPOSE:The chromosomal radiosensitivity of a selected group of familial breast cancer patients carrying a mutation in BRCA1 (n=11) or BRCA2 (n=9) and a group of healthy mutation carriers (n=12) was investigated and compared to a reference group of breast cancer patients without a BRCA1/2 mutation (n=78) and a group of healthy women carrying no mutation (n=58). MATERIALS AND METHODS:The chromosomal radiosensitivity was assessed with the G2 and the G0-micronucleus (MN)-assay on fresh blood samples and on Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines. For the MN-assay, lymphocytes were exposed in vitro to 3.5 Gy and 2 Gy 60Co gamma-rays at a high dose rate (HDR) or low dose rate (LDR). 70-h post-irradiation cultures were arrested and micronuclei were scored in 1000 binucleate cells. For the G2-assay lymphocytes were irradiated in vitro with a dose of 0.4 Gy 60Co gamma-rays after 71h incubation. Cultures were arrested 90 min after irradiation and chromatid breaks were scored in 50 metaphases. RESULTS:The group of breast cancer patients with a BRCA1 or 2 mutation was on average more radiosensitive than the control group, but not different from breast cancer patients without a BRCA mutation. The radiation response of healthy BRCA1/2 carriers was not significantly different from the control group and also not different from relatives without a BRCA mutation. Comparing the radiation response in EBV cell lines derived from breast cancer patients with or without a BRCA1 mutation revealed no significant difference. CONCLUSIONS:Our results reveal that chromosomal radiosensitivity observed in breast cancer patients heterozygous for BRCA1 or 2 mutations, could not be demonstrated in healthy BRCA1/2 mutation carriers. This suggests that mutations in BRCA1 or 2 genes are not playing a main role in chromosomal radiosensitivity, this although BRCA1 and 2 are both involved in DNA repair/signalling processes.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Baeyens A,Thierens H,Claes K,Poppe B,de Ridder L,Vral Adoi
10.1080/09553000400017937subject
Has Abstractpub_date
2004-10-01 00:00:00pages
745-56issue
10eissn
0955-3002issn
1362-3095journal_volume
80pub_type
杂志文章abstract::Radiologic accidents or terrorist acts involving radioactive material, as well as radiation exposure in medical or industrial procedures are potential sources of risk for human health. All these risks share a common element, exposure to ionizing radiation. The extent of ionizing radiation injury will depend on a numbe...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2019.1562254
更新日期:2019-05-01 00:00:00
abstract:PURPOSE:To determine the involvement of c-Jun NH(2)-terminal kinase-1 (JNK1) and possibly of HSP27 in heat-induced apoptosis of human monoblastic leukaemia U937 cells. MATERIALS AND METHODS:Dominant negative JNK1 (APF), in which the phosphorylation sites Thr-Pro-Tyr were changed to Ala-Pro-Phe, was overexpressed in U9...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110062512
更新日期:2001-08-01 00:00:00
abstract::Single crystals of cytosine hydrochloride with thiocytosine as an impurity were found suitable for the study of a possible new mechanism of long-range migration of energy deposited by ionizing radiation. In a crystal containing thiocytosine, two kinds of chlorine-containing paramagnetic centres are present that are co...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550891
更新日期:1994-07-01 00:00:00
abstract:PURPOSE:To investigate the action of ultra-low dose beta-radiation (ULDBR) on isolated segments of blood vessels. MATERIALS AND METHODS:We used the pharmacological model of isolated rabbit carotid arteries with intact or mechanically removed endothelium. Specific vascular responses to beta-irradiation were registered ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000600562229
更新日期:2005-11-01 00:00:00
abstract::The aim was to investigate further the relationship between radiation-induced mitotic delay and the expression of chromosome damage in V79 cells. Recently published data on the time-course of chromosome aberrations in V79 first-cycle metaphases after exposure to 10.4 MeV u(-1) Ar ions (LET = 1226 keV microm(-1)) were ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000400027902
更新日期:2005-01-01 00:00:00
abstract::Cell death induced by various radiation doses in Molt-4 and L5178Y cells was studied. Molt-4 cells have a low threshold for induction of interphase death while L5178Y cells have a high threshold. Interphase death in Molt-4 cells at doses > 100 Gy and in L5178Y cells at doses > 300 Gy showed typical necrosis. In contra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551101
更新日期:1993-07-01 00:00:00
abstract:PURPOSE:This review is a contribution to the memory of Dr William (Bill) Morgan and highlights an area of research and deliberation that he considered extremely important in support of the setting of protective radiation dose limits. Biological research has generally played a minor role in the estimation of adverse hea...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2017.1290848
更新日期:2017-10-01 00:00:00
abstract:PURPOSE:To study the oxidative stress-induced changes by sublethal doses of cadmium chloride (CdCl2) and gamma irradiation, two redox-inducing agents, on metallothionein (MT) gene and protein expression in Plantago ovata Forsk (P. ovata). MATERIALS AND METHODS:Chlorophyll content was estimated to study the stress resp...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.782109
更新日期:2013-07-01 00:00:00
abstract:PURPOSE:To investigate within the framework of a multilaboratory study the suitability of FISH chromosome painting to measure so-called stable translocations in peripheral lymphocytes of Mayak nuclear-industrial workers (from the Southern Urals) and their use for retrospective biodosimetry. MATERIALS AND METHODS:Chrom...
journal_title:International journal of radiation biology
pub_type: 杂志文章,多中心研究
doi:10.1080/09553000010018693
更新日期:2001-03-01 00:00:00
abstract:PURPOSE:To summarize current knowledge regarding mechanisms of radiation-induced skin injury and medical countermeasures available to reduce its severity. Advances in radiation delivery using megavoltage and intensity modulated radiation therapy have permitted delivery of higher doses of radiation to well-defined tumor...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.3109/09553002.2013.765055
更新日期:2013-05-01 00:00:00
abstract:PURPOSE:It has been previously argued that the use of the one-electron oxidants (SCN)2(*-) and Br2(*-) with plasmid DNA leads to the formation of DNA guanyl radicals. These guanyl radical species are intermediates in the DNA damage produced by processes such as photo-ionization and ionizing irradiation. The present pap...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110069119
更新日期:2001-11-01 00:00:00
abstract::Objective: To quantify DNA damage in patients undergoing non-contrast and contrast-enhanced 18F-FDG PET/CT whole body positron emission tomography/computed tomography (WB PET/CT) investigations using comet assay technique and micronucleus assay, and to study the effect of other baseline parameters of patients on DNA d...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1577569
更新日期:2019-06-01 00:00:00
abstract::A review is presented to the program of education and training setup within the DoReMi Network of Excellence. DoReMi was funded by Euratom under the EU 7th Framework Programme to coordinate the EU research into risks from low-dose ionizing radiation. It was seen to be necessary to form a network of expert institutions...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2018.1454616
更新日期:2019-01-01 00:00:00
abstract::Recent evidence indicates that isoeffect doses for spinal cord and kidney may be overestimated for fraction sizes as small as 1 or 2 Gy when calculated from a linear-quadratic (LQ) model fitted to data obtained for fraction sizes larger than 2 Gy. Reasons for this are unknown, but possible interpretations include exha...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814551461
更新日期:1988-07-01 00:00:00
abstract::SR 2508 (etanidazole) (1 mM) or buthionine sulphoximine (BSO, 50 microM) or both drugs together did not radiosensitize oxic V79 Chinese hamster cells irradiated at either an acute dose rate (2.35 Gy/min) or at a low dose rate (0.117 Gy/min). BSO pretreatment (15 h at 37 degrees C) depleted cellular glutathione (GSH) t...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550121
更新日期:1991-01-01 00:00:00
abstract::Purpose: Ionizing radiation is a risk factor to the whole organism, including the heart. Cardiac damage is considered to be a late effect of radiation exposure. While the acute cardiotoxicity of high doses is well characterized, the knowledge about nature and magnitude of the cardiac risk following lower doses exposur...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2020.1704299
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. MATERIALS AND METHODS:A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was esti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138745
更新日期:2000-03-01 00:00:00
abstract:PURPOSE:Chironomus ramosus is one of the recently reported radiotolerant insects. Salivary gland cells of fourth instar larvae respond to ionizing radiations with increases in the levels of antioxidant enzymes and chaperone proteins. Here we made an attempt to study the state of nuclear DNA after exposure of larvae to ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2015.1062572
更新日期:2015-01-01 00:00:00
abstract::It follows from the present work that the vascularization of the normal tissue present in the treatment volume limits the temperatures achieved during heat treatment of invasive ductal breast carcinoma. The temperatures can often be increased by giving fractionated heat treatment because heat treatment may reduce the ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114551821
更新日期:1991-07-01 00:00:00
abstract::This paper presents a summary of our understanding to date of the formation of DNA strand breaks induced by highly energetic particle beams (high-LET radiation). We have compared our own recent data on the formation of strand breaks induced in DNA in an aqueous solution with our previous data and those of others avail...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009014551431
更新日期:1990-07-01 00:00:00
abstract::The effects of UV radiation on humans and animals are receiving increasing attention and much interest has recently been focused on the environmental effects of UV A and UV B. This study compares the in vitro effects of UV A and UV B on the clonogenic survival of two human skin keratinocyte cell lines, HaCaT which are...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143590
更新日期:1997-07-01 00:00:00
abstract:PURPOSE:To study by fluorescence in situ hybridization (FISH) the involvement of Chinese hamster chromosomes 3, 4, 8, and 9, and their separate chromosome arms in X-ray-induced aberrations. MATERIALS AND METHODS:Male embryonic primary cells of Chinese hamster were used and metaphases were collected and scored at 20 h ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139700
更新日期:1999-08-01 00:00:00
abstract::The studies of the late effects of atomic-bomb (A-bomb) radiation on the immune system were started about 20 years after the bombings in 1945. The most remarkable late effects of radiation are the functional and quantitative abnormalities of T and B cells in survivors exposed to high doses (> or = 1.0 Gy). Abnormaliti...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009514551491
更新日期:1995-11-01 00:00:00
abstract:PURPOSE:To investigate whether extremely low frequency electromagnetic field (ELF-EMF) exposure could induce oxidative stress in workers performing tour-inspection near transformers and distribution power lines. MATERIALS AND METHODS:Occupational short-term 'spot' measurements were performed. In total, 310 inspection ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2015.1012304
更新日期:2015-05-01 00:00:00
abstract:PURPOSE:The effect of extremely low frequency electromagnetic fields (ELF-EMF) on bacteria has attracted attention due to its potential for beneficial uses. This research aimed to determine the effect of ELF-EMF on bacterial membrane namely the membrane potential, surface potential, hydrophobicity, respiratory activity...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2015.1101500
更新日期:2016-01-01 00:00:00
abstract::Existing models of chromosome aberrations produced by ionizing-radiation predict equal numbers of dicentrics and translocations if the dose is so low that complex aberrations can be ignored. We show that, for a specific subset of aberrations detected by FISH, dicentric/translocation equality is predicted even at highe...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145977
更新日期:1996-02-01 00:00:00
abstract:PURPOSE:To assess intestinal radioprotection by systemic or lumenal tirilazad mesylate and to test potential tumour radioprotection. MATERIALS AND METHODS:Segments of the mid small bowel were surgically exteriorized. Tirilazad was given either intravenously or intralumenally 30 min prior to 11 Gy of X-irradiation to t...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098142617
更新日期:1998-02-01 00:00:00
abstract:PURPOSE:To give a state-of-the-art overview on the promise of three-dimensional (3-D) culture systems for anticancer drug development, with particular emphasis on multicellular tumor spheroids (MCTS). RESULTS AND CONCLUSIONS:Cell-based assays have become an integral component in many stages of routine anti-tumor drug ...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000701727531
更新日期:2007-11-01 00:00:00
abstract::Photoirradiation of aqueous solutions of DNA in the presence of Al- or Zn-tetrasulphonated phthalocyanines (AIPcS4 and ZnPcS4) causes formation of strand breaks and liberation of nucleobases. The effect of added D2O, which enhances singlet oxygen (1O2) lifetime, radical scavengers including alcohols and the spin-trap ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::DNA neutral (pH 9.6) filter elution was used to measure radiation-induced DNA double-strand break (dsb) frequencies in eight human squamous cell carcinoma cell lines with radiosensitivities (D0) ranging from 1.07 to 2.66 Gy and D values ranging from 1.46 to 4.08 Gy. The elution profiles of unirradiated samples from mo...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114551211
更新日期:1991-06-01 00:00:00