Abstract:
:Human dermal fibroblasts (HDFs) regulate wound-healing processes in human skin, including the regeneration of skin sensory fibres, in response to various mechanical stimuli. Because nerve growth factor (NGF) has an essential role in sensory regeneration, we evaluated the possible association of NGF with mechanical stimulus-dependent cellular responses in HDFs. A cyclic tensile stimulus increased both NGF and transforming growth factor (TGF) β2 production, yet with different gene transcription and signal desensitization profiles. Neutralizing TGFβ with antibodies did not affect the tension-induced NGF upregulation, with significant inhibition of endogenous TGFβ2 transcription. The treatment with LY294002, SP600125 or U0126 hindered the tension-induced TGFβ2 upregulation, although the increase in NGF was regulated only by SP600125 or U0126, indicating the involvement of three signalling kinase pathways in the upregulation of TGFβ2. However, the upregulation of NGF was shown to be independent of PI3K, demonstrating the independent regulation of tension-induced NGF and TGFβ production in HDFs.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Kim M,Shin DW,Shin H,Noh M,Shin JHdoi
10.1111/exd.12064subject
Has Abstractpub_date
2013-01-01 00:00:00pages
72-4issue
1eissn
0906-6705issn
1600-0625journal_volume
22pub_type
信件abstract::Reverse transcription polymerase chain reaction (qRT-PCR) has become a mainstay in many areas of skin research. To enable quantitative analysis, it is necessary to analyse expression of reference genes (RGs) for normalization of target gene expression. The selection of reliable RGs therefore has an important impact on...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12759
更新日期:2015-10-01 00:00:00
abstract::The role of angiogenesis in tumour growth and metastasis is well established. However, investigations of tumour microcirculation to date have used either biopsy material from human tumours, or animal models in vivo. We have studied the tumour microcirculation in vivo in human skin cancers using video-microscopy to exa...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.1999.tb00374.x
更新日期:1999-06-01 00:00:00
abstract::Filaggrin (FLG) loss-of-function (LOF) variants are a major risk factor for the common inflammatory skin disease, atopic dermatitis (AD) and are often population-specific. African-American (AA) children are disproportionately affected with AD, often later developing asthma and/or allergic rhinitis and comprise an atop...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13691
更新日期:2018-09-01 00:00:00
abstract::Pruritus is the most distressing symptom in haemodialysis (HD) patients. Its aetiology has not yet been delineated, and thus there are no good therapeutical options. Case reports and series attribute antipruritic potency to the serotonin receptor antagonists of the 5-HT3 type in renal pruritus. It was the aim of this ...
journal_title:Experimental dermatology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.0906-6705.2004.00184.x
更新日期:2004-05-01 00:00:00
abstract::Mast cells (MCs) have been intensely investigated over the past two decades, e.g. the numbers of PubMed-listed reports on MCs have steadily increased and doubled over the past twenty years. Surprisingly, many recent findings that have fundamentally changed our understanding of MC biology and functions have yet to be s...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0625.2005.00369.x
更新日期:2005-12-01 00:00:00
abstract::Subcutaneous mast cells (MCs) are vulnerable to mechanical stimulation from external environment. Thus, MCs immune function could be modulated by their mechanosensitivity. This property has been identified as the trigger mechanism of needling acupuncture, a traditional oriental therapy. Previously we have demonstrated...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.14093
更新日期:2020-05-01 00:00:00
abstract::Despite recent success in melanoma therapy, most patients with metastatic disease still undergo deadly progression. We have identified a novel mechanism of multidrug resistance allowing a small subpopulation of slow-cycling melanoma cells to survive based on elevated oxidative bioenergy metabolism. In this study, we a...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/exd.12613
更新日期:2015-02-01 00:00:00
abstract::Despite groundbreaking new treatments such as checkpoint inhibition and targeted therapy, the overall response and survival rates are limited in patients with metastatic melanoma. Here, we hypothesize that melatonin and its metabolites could be promising boosters of the efficacy of BRAF/MEK inhibitors in patients with...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.14144
更新日期:2020-09-01 00:00:00
abstract::In the last decades melanoma incidence has been increasing worldwide, while mortality remained on a high level. Until now, there is no suitable therapy for metastasized melanoma, which could lead to a significant increase in overall survival. Apoptosis deficiency is supposed to be a critical factor for therapy resista...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0625.2007.00655.x
更新日期:2008-01-01 00:00:00
abstract::Type VII collagen is the major component of anchoring fibrils in the epidermal basement membrane. Its expression has been analyzed by immunostaining or Northern blotting, but rarely at the protein level. In this study, we have quantitatively examined the effects of ascorbic acid and various cytokines/growth factors on...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2006.00514.x
更新日期:2007-02-01 00:00:00
abstract::Dermal mast cells protect the skin from inflammatory effects of ultraviolet (UV) radiation and are required for UV-induced immune suppression. We sought to determine a potential mechanistic role of mast cells in reducing the sensitivity to UV radiation (i.e. phototolerance induction) through photohardening. We adminis...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12687
更新日期:2015-07-01 00:00:00
abstract::Aberrant mitochondrial structure and function influence tissue homeostasis and thereby contribute to multiple human disorders and ageing. Ten per cent of patients with primary mitochondrial disorders present skin manifestations that can be categorized into hair abnormalities, rashes, pigmentation abnormalities and acr...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/exd.12484
更新日期:2014-09-01 00:00:00
abstract::Epidermolytic hyperkeratosis (EHK) is a hereditary skin disorder typified by blistering due to cytolysis. One in 100,000 individuals is affected by this autosomal-dominant disease. The onset of the disease phenotype is typically at birth. Histological and ultrastructural examination of the epidermis shows a thickened ...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1034/j.1600-0625.2000.009001016.x
更新日期:2000-02-01 00:00:00
abstract::Pachyonychia congenita type 1 (PC-1) is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy, focal non-epidermolytic palmoplantar keratoderma and variable features of oral leukokeratosis and follicular keratosis. Previously, we have shown that this disease can be caused by mutations...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.1999.tb00356.x
更新日期:1999-04-01 00:00:00
abstract::More than 40 null mutations in the filaggrin (FLG) gene are described. It is therefore possible to find two different null mutations in one individual (compound heterozygosity). It has been generally perceived that homozygous and compound heterozygous individuals were genotypically comparable; however, this has not be...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12199
更新日期:2013-09-01 00:00:00
abstract::It has been suggested that the increased rate of bacterial infection in atopic dermatitis (AD) may be caused by reduced antimicrobial protein (AMP) expression. We were interested whether common treatments in AD affect antimicrobial defense. We investigated the effects of topically applied corticosteroids betamethasone...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2011.01322.x
更新日期:2011-10-01 00:00:00
abstract::It has been reported that the abnormal regulation of melanocyte stem cells (McSCs) causes hair greying; however, little is known about the role of McSCs in skin hyperpigmentation such as solar lentigines (SLs). To investigate the involvement of McSCs in SLs, the canonical Wnt signalling pathway that triggers the diffe...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12496
更新日期:2014-09-01 00:00:00
abstract::Earlier studies showed that dermal cells lose trichogenic capacity with passage, but studies on the effect of keratinocyte passage on human hair follicle neogenesis and graft quality have been hampered by the lack of a suitable model system. We recently documented human hair follicle neogenesis in grafted dermal-epide...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/exd.12424
更新日期:2014-06-01 00:00:00
abstract::Primary cutaneous large B-cell lymphomas, leg type (PCLBCL/LT) are primary cutaneous B-cell lymphoma (PCBCL) with an intermediate prognosis. Therefore, antracycline-based polychemotherapy combined with rituximab has been recommended as first-line treatment. Yet, despite this regimen, the 5-year survival rate remains 5...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12688
更新日期:2015-06-01 00:00:00
abstract::Chimeric antigen receptor (CAR)-T cells are one of the impressive recent success stories of anti-cancer immunotherapy. Especially in haematological malignancies, this treatment strategy has shown promising results leading to the recent approval of two CAR-T cell constructs targeting CD19 in the United States and the E...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13792
更新日期:2018-12-01 00:00:00
abstract::In the last decade, significant progress has been made in understanding skin cancer cell death resistance mechanisms, and a number of new treatment strategies have been developed. Systematic approach genomic studies of various cancer types have opened new possibilities for the development of anticancer therapies. Howe...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13309
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVE:To evaluate the long-term efficacy of etanercept for the management of hidradenitis suppurativa. METHODS:Analysis was based on the long-term follow-up (weeks 24-144) of 10 patients enrolled in a prospective open-label phase II study; etanercept was initially administered subcutaneously 50 mg once weekly for ...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/j.1600-0625.2009.00967.x
更新日期:2010-06-01 00:00:00
abstract::Dermal papilla cells (DPC) control the growth character of the hair follicle through their elaboration of mitogenic factors and extracellular matrix components. Further, the dermal papilla is a primary site of androgen action in the hair follicle. Interleukin-1alpha (IL-1alpha) is prominent in skin wounding and inflam...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2006.00462.x
更新日期:2006-10-01 00:00:00
abstract::The generation of reactive oxygen species is among the various mechanisms by which ultraviolet radiation damages skin. Tempol, a superoxide dismutase analogue which readily penetrates cell membranes when administered exogenously, has been shown to provide protection against some forms of oxygen-dependent damage. In th...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1034/j.1600-0625.2001.100107.x
更新日期:2001-02-01 00:00:00
abstract:BACKGROUND:The accuracy of diagnosis of skin cancer and especially of early malignant melanoma is most important to reduce its morbidity and mortality. Previous pilot studies using electrical impedance measurements indicate statistically significant accuracies for the detection of skin cancer. OBJECTIVES:The aim of th...
journal_title:Experimental dermatology
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1600-0625.2011.01285.x
更新日期:2011-08-01 00:00:00
abstract::CD44 is a family of cell-surface receptors on human lymphocytes that act as co-stimulatory molecules leading to the induction of effector functions in T cells. We have analyzed primary cutaneous malignant melanomas with clinical and histologic signs of tumor regression using immunohistochemistry and observed the predo...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1034/j.1600-0625.2003.00044.x
更新日期:2003-04-01 00:00:00
abstract::Primary hyperhidrosis is characterized by excessive sweating in palmar, plantar and axillary body regions. Gland hypertrophy and the existence of a third type of sweat gland, the apoeccrine gland, with high fluid transporting capabilities have been suggested as possible causes. This study investigated whether sweat gl...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/j.1600-0625.2011.01361.x
更新日期:2011-12-01 00:00:00
abstract::Keloid is a dermal proliferative disorder characterized by the excessive proliferation and migration of keratinocytes and fibroblasts. Over-activation of the serine/threonine protein kinase, mammalian target of rapamycin (mTOR), plays a pivotal role in the process. Here, we show that both mTOR complex 1 (mTORC1) and m...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13882
更新日期:2019-03-01 00:00:00
abstract::Card9 is a signalling adaptor protein in the downstream of many innate pattern recognition receptors (PRRs) and exerts a significant role in antifungal immunity. To date, Card9 deficiency has been reported to be related to increased susceptibility to many fungal infections. In this study, we established mucormycosis m...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.14020
更新日期:2019-11-01 00:00:00
abstract::We have developed a new technique that rapidly and reproducibly allows direct visualization of molecular interactions, including receptor-ligand binding. The technique can be easily applied to examine binding between proteins and glycoproteins, or proteins and glycolipids, including gangliosides. In this novel bead-bi...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1034/j.1600-0625.2002.110510.x
更新日期:2002-10-01 00:00:00