Abstract：
:We have developed a new technique that rapidly and reproducibly allows direct visualization of molecular interactions, including receptor-ligand binding. The technique can be easily applied to examine binding between proteins and glycoproteins, or proteins and glycolipids, including gangliosides. In this novel bead-binding assay, the suspected 'ligand' molecule is bound to 0.2- or 1.0- micro m colored FluoSphere beads. These coated beads are then mixed directly with 150- micro m Sepharose or 50- to 75- micro m agarose beads coated with the second 'target' molecule. Binding between molecules is easily detected by immunofluoresence microscopy as colored rosettes or aggregations formed by clustering of the smaller fluorescent beads around the larger non-fluorescent bead. The validity of this technique for glycolipid binding to protein was verified through demonstration of the known interaction between the beta subunit of cholera toxin with ganglioside GM1. The bead-binding technique facilitated the novel observations of interaction between ganglioside GT1b with the alpha5 subunit of alpha5beta1 integrin and the interaction of GM3 with the epidermal growth factor receptor. A modification of this technique, in which the coated beads are bound to protein fixed on plates, allows a quantifiable colorimetric assay of interaction. This versatile and rapid technique will have widespread applications for in vitro systems and may also be useful for in vivo analysis of binding to cell surface receptor molecules.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Irvine AD,Sun P,Kos L,Wang XQ,Paller ASdoi
10.1034/j.1600-0625.2002.110510.xsubject
Has Abstractpub_date
2002-10-01 00:00:00pages
462-7issue
5eissn
0906-6705issn
1600-0625pii
exd110510journal_volume
11pub_type
杂志文章abstract：:Topical photodynamic therapy with 5-aminolevulinic acid (5-ALA) is a newly established treatment modality for epithelial skin cancer. Skin distribution of different 20% 5-ALA formulations was investigated by detection of protoporphyrin IX (PpIX) using an ex vivo porcine skin model. Fluorescence of PpIX was first detec...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.2008.00705.x
更新日期：2008-10-01 00:00:00
abstract：:Claudins (Cldn) are the major components of tight junctions (TJs) sealing the paracellular cleft in tissue barriers of various organs. Zebrafish Cldnb, the homolog of mammalian Cldn4, is expressed at epithelial cell-cell contacts and is important for regulating epidermal permeability. The bacterial toxin Clostridium p...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/exd.12728
更新日期：2015-08-01 00:00:00
abstract：:Tetracyclines (TCN) have powerful anti-inflammatory properties in addition to their anti-microbial effects. These anti-inflammatory effects are thought to play a role in inhibiting cutaneous inflammation in patients with rosacea and acne; however, the mechanism(s) of this action remains poorly understood. We have prev...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.2008.00716.x
更新日期：2008-09-01 00:00:00
abstract：:The primary trigger of polymorphic light eruption (PLE) remains to be uncovered. We hypothesize that PLE may be initiated by elements resulting from UV-induced damage to microbial communities of the skin, leading to a cascade of events eventually resulting in the skin rash of the disease. One mechanism by which epider...
journal_title：Experimental dermatology
pub_type： 信件
doi：10.1111/exd.13162
更新日期：2016-12-01 00:00:00
abstract：:Ultraviolet (UV) radiation contained in sunlight is considered a major risk in the induction of skin cancer. While mast cells are best known for their role in allergic responses, they have also been shown to play a crucial role in suppressing the anti-tumour immune response following UV exposure. Evidence is now emerg...
journal_title：Experimental dermatology
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0625.2012.01438.x
更新日期：2012-04-01 00:00:00
abstract：:CD4+ Foxp3+ regulatory T cells (Tregs) are suppressors of immune activation and play a crucial role in the maintenance of peripheral tolerance. Mutations of Foxp3 result in fatal autoimmunity in multiple organs, including the skin, in both humans and mice. Many studies have demonstrated the altered frequency and funct...
journal_title：Experimental dermatology
pub_type： 杂志文章,评审
doi：10.1111/exd.13535
更新日期：2019-06-01 00:00:00
abstract：:Patients with the blistering disease, epidermolysis bullosa (EB), frequently suffer from chronic wounds that become colonized by pathogenic bacteria, such as Staphylococcus aureus. To determine S. aureus colonization rates in patients with EB, swabs were collected from the anterior nares, throats and wounds of 52 Dutc...
journal_title：Experimental dermatology
pub_type： 信件
doi：10.1111/j.1600-0625.2012.01502.x
更新日期：2012-06-01 00:00:00
abstract：:The generation of a robust CD8(+) cytotoxic T lymphocyte (CTL) response is a key feature of many immunotherapeutic strategies against epithelial tumors and virally infected epithelial tissue. However, surprisingly few studies have addressed whether primary epithelial cells, expressing defined endogenous antigens, are ...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.2009.00907.x
更新日期：2009-12-01 00:00:00
abstract：:Recently, 3% diclofenacnatrium gel (diclofenac) was introduced for the treatment of actinic keratoses. Data on photocarcinogenesis of topical diclofenac are limited, and we wished to investigate whether topical diclofenac can accelerate photocarcinogenesis using simulated solar radiation (SSR). Diclofenac was applied ...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.2010.01215.x
更新日期：2011-04-01 00:00:00
abstract：:Inherited skin fragility comprises disorders characterized by mechanical induced blistering and erosions within the skin and mucosal membranes as a consequence of mutations in genes encoding proteins involved in intra-epidermal or dermal-epidermal adhesion. As the molecular pathology is largely known, it is a prototyp...
journal_title：Experimental dermatology
pub_type： 杂志文章,评审
doi：10.1111/exd.12666
更新日期：2015-05-01 00:00:00
abstract：:The anti-inflammatory mechanisms of adapalene, a synthetic retinoid used for the treatment of acne patients, are partially understood. They seem particularly related to the modulation of the non-specific immunity. Recent studies have shown that Toll-like receptor (TLR)-2 expression, a receptor of the innate immune sys...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.2007.00552.x
更新日期：2007-06-01 00:00:00
abstract：:Tyrosine kinase inhibitors of the tyrphostin family which block EGF receptor kinase are reported to arrest the growth of psoriatic keratinocytes in vitro. Three tyrphostins with the potency ratio AG555 > AG18 > AG814 were found to arrest growth with no adverse cytotoxic effects. The potency ratio to inhibit keratinocy...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.1995.tb00227.x
更新日期：1995-04-01 00:00:00
abstract：INTRODUCTION:The aetiology of mycosis fungoides and parapsoriasis (which may be considered as an early stage of mycosis fungoides) remains debated. Previous recent studies have suspected the involvement of viral agents and particularly human herpes viruses (HHV).The aim of the present study was to screen for the presen...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.2008.00787.x
更新日期：2009-04-01 00:00:00
abstract：:Diminished suppressive capacity of regulatory T cells (Treg) has been demonstrated in blood and in lesional skin of psoriatic patients. Treatment with anti-TNFα restored the number and function of circulating Treg in psoriasis. We aimed to study Treg in the skin of psoriatic patients undergoing topical treatment with ...
journal_title：Experimental dermatology
pub_type： 信件
doi：10.1111/exd.12575
更新日期：2015-01-01 00:00:00
abstract：:The present study was designed to examine the expression of proopiomelanocortin (POMC) and its related derivative peptide adrenocorticotropic hormone (ACTH) in murine derived Thy-1+ dendritic cells. Immunostaining using a polyclonal antibody specific to ACTH and parent POMC molecule indicated the presence of POMC and ...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.1995.tb00208.x
更新日期：1995-10-01 00:00:00
abstract：:Ultraviolet (UV) irradiation on skin triggers photoageing-related phenotypes such as formation of wrinkles. UV ray upregulates matrix metalloproteinase-1 (MMP-1), which in turn degrades extracellular matrix proteins, mostly collagens. Serum amyloid A1 (SAA1) is an acute-phase protein of which plasma concentration incr...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/exd.12979
更新日期：2016-07-01 00:00:00
abstract：:It has been reported that the abnormal regulation of melanocyte stem cells (McSCs) causes hair greying; however, little is known about the role of McSCs in skin hyperpigmentation such as solar lentigines (SLs). To investigate the involvement of McSCs in SLs, the canonical Wnt signalling pathway that triggers the diffe...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/exd.12496
更新日期：2014-09-01 00:00:00
abstract：:The immune system is called into action by alarm signals generated from injured tissues. We examined the nature of these alarm signals after exposure of skin residential cells to contact allergens (nickel sulfate and potassium dichromate) and a contact irritant [sodium dodecyl sulfate (SDS)]. Nickel sulfate, potassium...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.0906-6705.2005.00226.x
更新日期：2005-02-01 00:00:00
abstract：:Despite recent success in melanoma therapy, most patients with metastatic disease still undergo deadly progression. We have identified a novel mechanism of multidrug resistance allowing a small subpopulation of slow-cycling melanoma cells to survive based on elevated oxidative bioenergy metabolism. In this study, we a...
journal_title：Experimental dermatology
pub_type： 信件
doi：10.1111/exd.12613
更新日期：2015-02-01 00:00:00
abstract：:Serum levels of B-cell activating factor belonging to the tumor necrosis factor family (BAFF), a potent B-cell survival factor, are elevated in patients with systemic autoimmune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis and systemic sclerosis (SSc). The objective of this study was to d...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.2006.00485.x
更新日期：2007-02-01 00:00:00
abstract：:Contact allergens induce several accessory signals which promote the activation of antigen-specific T cells. One of these signals is the increased expression of adhesion molecules on antigen-presenting cells and endothelial cells. Epicutaneous application of non-toxic doses of 2,4-dinitrofluorobenzene (DNFB) onto the ...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.1992.tb00188.x
更新日期：1992-11-01 00:00:00
abstract：:Vitiligo is a chronic skin disease characterized by the appearance of zones of depigmentation. It is mostly described as an autoimmune disease in which the immune system destroys the melanocytes. Consistent with this origin, genetic studies have implicated genes encoding proteins mediating the immune response targetin...
journal_title：Experimental dermatology
pub_type： 杂志文章,评审
doi：10.1111/exd.13858
更新日期：2019-06-01 00:00:00
abstract：:Ultraviolet radiation (UVR) is known to be involved in the initiation and progression of malignant melanoma. Many studies have focused on the initiation of melanoma, but less is known about the effect of UVR on established tumor cells. Here, we show that after ultraviolet-B (UVB) irradiation, melanoma cells (MM) are a...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.2007.00572.x
更新日期：2007-08-01 00:00:00
abstract：:We aimed to evaluate the effect of small interfering RNA (siRNA) targeting CTGF on extracellular matrices (ECMs) metabolism in normal and SSc fibroblasts. Normal and SSc fibroblasts were transfected with CTGF-specific siRNAs to silence CTGF synthesis. After silencing CTGF, production of type I collagen and matrix meta...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.2009.00999.x
更新日期：2010-08-01 00:00:00
abstract：:The ability to manipulate the mammalian hair cycle will lead to novel therapies and strategies to combat all forms of alopecia. Thus, in addition to the epithelial-mesenchymal interactions in the hair follicle, niche and microenvironmental signals that accompany the phases of growth, regression and rest need to be scr...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/exd.14070
更新日期：2020-03-01 00:00:00
abstract：:The role of angiogenesis in tumour growth and metastasis is well established. However, investigations of tumour microcirculation to date have used either biopsy material from human tumours, or animal models in vivo. We have studied the tumour microcirculation in vivo in human skin cancers using video-microscopy to exa...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.1999.tb00374.x
更新日期：1999-06-01 00:00:00
abstract：:Psychological factors are believed to play a role in the pathogenesis of alopecia areata (AA), a frequently encountered hair disorder. In our study, statistically significant elevation of psychological stress was felt by AA patients prior hair loss compared with control, which was strongly believed contributory to hai...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.2006.00443.x
更新日期：2006-07-01 00:00:00
abstract：:Pseudoxanthoma elasticum (PXE), a heritable multi-system disorder, is caused by mutations in the ABCC6 gene primarily expressed in the liver. Recent analysis of cultured fibroblasts from patients with PXE has suggested compensatory alterations in the expression of the ABCA-subfamily of genes. We have now determined by...
journal_title：Experimental dermatology
pub_type： 信件
doi：10.1111/j.1600-0625.2010.01240.x
更新日期：2011-05-01 00:00:00
abstract：:To determine the therapeutic mechanism of PUVA in psoriasis vulgaris, the effects of PUVA on activated T lymphocytes were investigated in vitro. Peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers were activated with Con A stimulation (Con A blasts). Both untreated PBMC and Con A blasts were irr...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.1995.tb00219.x
更新日期：1995-02-01 00:00:00
abstract：:Pro- and anti-apoptotic members of the Bcl-2 family are fundamental in the control of apoptosis. Among them, Bax plays a key role in apoptosis induction by mediating the release of apoptogenic factors from mitochondria to the cytosol. In this report, we investigated, by immunohistofluorescence, the in vivo distributio...
journal_title：Experimental dermatology
pub_type： 杂志文章
doi：10.1111/j.1600-0625.2007.00660.x
更新日期：2008-02-01 00:00:00
