Abstract:
:Earlier studies showed that dermal cells lose trichogenic capacity with passage, but studies on the effect of keratinocyte passage on human hair follicle neogenesis and graft quality have been hampered by the lack of a suitable model system. We recently documented human hair follicle neogenesis in grafted dermal-epidermal composites, and in the present study, we determined the effects of keratinocyte passage on hair follicle neogenesis. Dermal equivalents were made with cultured human dermal papilla cells and were overlaid with either primary or passaged human keratinocytes to form dermal-epidermal composites; these were then grafted onto immunodeficient mice. Superior hair follicle neogenesis was observed using early keratinocyte cultures. Characteristics such as formation of hair shafts and sebaceous glands, presence of hair follicles with features of anagen or telogen follicles, and reproducible hair and skin function parameters make this model a tool to study human hair follicle neogenesis and development.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Thangapazham RL,Klover P,Li S,Wang JA,Sperling L,Darling TNdoi
10.1111/exd.12424subject
Has Abstractpub_date
2014-06-01 00:00:00pages
443-6issue
6eissn
0906-6705issn
1600-0625journal_volume
23pub_type
信件abstract::Tetracyclines (TCN) have powerful anti-inflammatory properties in addition to their anti-microbial effects. These anti-inflammatory effects are thought to play a role in inhibiting cutaneous inflammation in patients with rosacea and acne; however, the mechanism(s) of this action remains poorly understood. We have prev...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2008.00716.x
更新日期:2008-09-01 00:00:00
abstract::For almost a quarter of a century, ex vivo studies of human scalp hair follicles (HFs) have permitted major advances in hair research, spanning diverse fields such as chronobiology, endocrinology, immunology, metabolism, mitochondrial biology, neurobiology, pharmacology, pigmentation and stem cell biology. Despite thi...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/exd.12836
更新日期:2015-12-01 00:00:00
abstract::Primary hyperhidrosis is characterized by excessive sweating in palmar, plantar and axillary body regions. Gland hypertrophy and the existence of a third type of sweat gland, the apoeccrine gland, with high fluid transporting capabilities have been suggested as possible causes. This study investigated whether sweat gl...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/j.1600-0625.2011.01361.x
更新日期:2011-12-01 00:00:00
abstract::Type VII collagen is the major component of anchoring fibrils in the epidermal basement membrane. Its expression has been analyzed by immunostaining or Northern blotting, but rarely at the protein level. In this study, we have quantitatively examined the effects of ascorbic acid and various cytokines/growth factors on...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2006.00514.x
更新日期:2007-02-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding RNA molecules, which function in RNA silencing and post-transcriptional regulation of gene expression. Psoriasis is an inflammatory skin disease characterized by the dysfunction of keratinocytes, with the immune dysregulation. We reviewed the recent studies on the roles of miRNA...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/exd.13249
更新日期:2017-04-01 00:00:00
abstract::Allergen-induced emigration and maturation of dendritic cells (DC) are pivotal steps in sparking off allergic contact dermatitis. In vitro models, reflecting these steps, may provide tools for assessment of sensitizing capacities of putative contact allergens. Here, we evaluated the applicability of such models for a ...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1034/j.1600-0625.2003.00077.x
更新日期:2003-10-01 00:00:00
abstract::Reverse transcription polymerase chain reaction (qRT-PCR) has become a mainstay in many areas of skin research. To enable quantitative analysis, it is necessary to analyse expression of reference genes (RGs) for normalization of target gene expression. The selection of reliable RGs therefore has an important impact on...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12759
更新日期:2015-10-01 00:00:00
abstract::The high-frequency electrical conductance of tape-stripped human skin in vivo can be used to evaluate the hydration profile of Stratum corneum (SC). Tape-stripping provides access to the underlying SC layers, and the conductance of these layers (as measured by the Skicon instrument) correlates well with their water co...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2009.00868.x
更新日期:2009-10-01 00:00:00
abstract::The skin forms an effective barrier between the organism and the environment preventing invasion of pathogens and fending off chemical and physical assaults, as well as the unregulated loss of water and solutes. In this review we provide an overview of several components of the physical barrier, explaining how barrier...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0625.2008.00786.x
更新日期:2008-12-01 00:00:00
abstract::Second-harmonic-generation (SHG) microscopy is a powerful tool for in vivo visualisation of collagen fibres in human skin because of its specific collagen selectivity without the need for staining, non-invasiveness and high-resolution three-dimensional imaging. Although texture analysis of SHG images is a promising me...
journal_title:Experimental dermatology
pub_type: 临床试验,杂志文章
doi:10.1111/exd.13560
更新日期:2019-08-01 00:00:00
abstract::In the last decade, significant progress has been made in understanding skin cancer cell death resistance mechanisms, and a number of new treatment strategies have been developed. Systematic approach genomic studies of various cancer types have opened new possibilities for the development of anticancer therapies. Howe...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13309
更新日期:2017-10-01 00:00:00
abstract::Flavonoids belong to a large group of polyphenolic compounds that are widely present in plants. Certain flavonoids, including naringenin, have cytoprotective properties. Although the antioxidant effect has long been thought to be a crucial factor accounting for the cellular effects of flavonoids, mitochondrial channel...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13903
更新日期:2019-05-01 00:00:00
abstract::Mycosis fungoides and its leukaemic counterpart Sézary syndrome are the most frequent cutaneous T-cell lymphomas (CTCL), and there is no cure for these diseases. We evaluated the effect of clinically approved antihistamines on the growth of CTCL cell lines. CTCL cell lines as well as blood lymphocytes from patients wi...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12086
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Methylenetetrahydrofolate reductase (MTHFR) is involved in the formation of methyl donors, which contribute to DNA methylation. DNA methylation is an essential epigenetic feature playing a critical role in gene regulation and cellular differentiation. In addition, MTHFR activity affects plasma homocysteine l...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2008.00713.x
更新日期:2008-09-01 00:00:00
abstract::Chronic spontaneous urticaria (CSU) is a frequent disorder with recurrent itchy wheals and/or angioedema, and nearly 35% patients respond poorly to non-sedating H1 antihistamine treatment. CRP gene encodes the C-reactive protein, which is involved in the pathogenesis of CSU. To investigate the impacts of CRP polymorph...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13874
更新日期:2019-03-01 00:00:00
abstract::Polychlorinated biphenyls (PCBs) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are major environmental pollutants, and their effects on the human body critically depend on the aryl hydrocarbon receptor (AhR). The aim of this study was to evaluate the significance of the AhR and its ligands in chronic inflammatory ski...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/exd.12350
更新日期:2014-04-01 00:00:00
abstract::Skin irritation is one of the most common adverse reactions in hydroquinone (HQ) and retinoic acid (RA). Although melanocytes have rarely been considered to be involved in skin irritation, RA and particularly HQ could induce melanocyte toxicity, resulting in depigmentation. We chose S100B as a candidate gene for melan...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12332
更新日期:2014-03-01 00:00:00
abstract::The adoptive transfer of engineered T cells represents an important approach in immunotherapy of melanoma. However, relapse of the tumor can occur due to immune-escape mechanisms developed by the tumor cells, for example antigen loss, downregulation of the major histocompatibility complex presentation machinery and de...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13095
更新日期:2016-11-01 00:00:00
abstract::4-(4-Hydroxyphenyl)-2-butanol (rhododendrol, RD), a skin-whitening agent, was reported to cause skin depigmentation in some users, which is attributed to its cytotoxicity to melanocyte. It was reported that cytotoxicity to melanocyte is possibly mediated by oxidative stress in a tyrosinase activity-dependent manner. W...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13555
更新日期:2018-07-01 00:00:00
abstract::Cowden syndrome (CS) is an autosomal dominant inherited disorder characterized by hamartomas in a variety of tissues including the skin, thyroid, breast, endometrium, and the brain. Individuals with CS are predisposed to development of malignancy in these organs, especially the breast and the thyroid. We describe 3 un...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1034/j.1600-0625.2000.009002152.x
更新日期:2000-04-01 00:00:00
abstract::Ultraviolet B (UVB) radiation induces inflammation in the skin specifically at the site of exposure. We unexpectedly found that UVB-induced inflammation was not induced in gp91phox-depleted mice. To test whether gp91phox is directly involved in UVB-induced inflammation, neutrophil- and hyaluronic acid-depleted mice we...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12032
更新日期:2012-12-01 00:00:00
abstract::Exposure of the skin to ultraviolet (UV) irradiation induces photoageing through the up-regulation of matrix metalloproteinases (MMPs) and subsequent breakdown of extracellular matrices. Reactive oxygen species (ROS) and epidermal growth factor receptor (EGFR) activation play central roles in UV-induced MMP expression...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12138
更新日期:2013-05-01 00:00:00
abstract::There is increasing awareness that psoriasis is more than 'skin deep'. Several recent reviews focussed on biomarkers indicating the systemic dimension of psoriasis and the aspect of comorbidity psoriasis shares with other chronic inflammatory diseases, such as Crohn's disease and rheumatoid arthritis. Of emerging sign...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2011.01261.x
更新日期:2011-04-01 00:00:00
abstract::We investigated the effects of a single dose of mouse interleukin-31 (IL-31) on scratching behaviour in comparison with spontaneous skin-lesion- or serotonin (5-HT)- induced scratching behaviour in NC/Nga and BALB/c mice. Intradermal (i.d.) injection of IL-31 caused a gradual increase in long-lasting scratching (LLS, ...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12222
更新日期:2013-10-01 00:00:00
abstract::In the area of dermatological research, the knowledge of rate and extent of substance penetration into the human skin is essential not only for evaluation of therapeutics, but also for risk assessment of chemicals and cosmetic ingredients. Recently, confocal Raman microscopy emerged as a novel analytical technique for...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/exd.12542
更新日期:2014-12-01 00:00:00
abstract::Both CCL27 and CCL28 are ligands for CCR10 and attract CCR10(+) lymphocytes. We previously demonstrated that CCL27 and CCL28 were strongly expressed in sera and lesional keratinocytes of patients with atopic dermatitis and psoriasis vulgaris. However, the regulation of CCL27 and CCL28 production in keratinocytes has n...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2005.00390.x
更新日期:2006-02-01 00:00:00
abstract::The 'matrikine' concept claims that processing of the precursors for collagen results in the formation of peptides such as KTTKS which in turn augments extracellular matrix (ECM) production. In the present study, we show the development of an anti-ageing active from an in silico approach by molecular design resulting ...
journal_title:Experimental dermatology
pub_type: 信件,随机对照试验
doi:10.1111/j.1600-0625.2011.01307.x
更新日期:2011-07-01 00:00:00
abstract::In recent years, hyaluronan (HA) has become an increasingly attractive substance as a non-immunogenic filler and scaffolding material in cosmetic dermatology. Despite its wide use for skin augmentation and rejuvenation, relatively little is known about the molecular structures and interacting proteins of HA in normal ...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/exd.12370
更新日期:2014-05-01 00:00:00
abstract::Recently, decreased interferon-gamma (IFN-gamma) and increased interleukin (IL)-4 production have been reported in measurements of the content of the cytokines in culture supernatants of peripheral blood mononuclear cells (PBMC) obtained from patients with atopic dermatitis (AD). These data suggest deviation of PBMC i...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.1998.tb00310.x
更新日期:1998-04-01 00:00:00
abstract::Solar ultraviolet (UV) radiation has hazardous effects on human health that are, in part, associated with its immunosuppressive effects via the induction of interleukin (IL)-10 production. Although IL-10 is produced by both T helper type 2 (Th2) cells and T-regulatory type 1 (Tr1) cells, the relative contribution of e...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2006.00452.x
更新日期:2006-08-01 00:00:00