Abstract：
:Rotavirus (RV) and norovirus (NoV) are the two most important causes of viral gastroenteritis. While vaccine remains an effective prophylactic strategy, development of other approaches, such as passive immunization to control and treat clinical infection and illness of these two pathogens, is necessary. Previously we demonstrated that high titers of NoV-specific IgY were readily developed by immunization of chickens with the NoV P particles. In this study, we developed a dual IgY against both RV and NoV through immunization of chickens with a divalent vaccine comprising neutralizing antigens of both RV and NoV. This divalent vaccine, named P-VP8(∗) particle, is made of the NoV P particle as a carrier with the RV spike protein VP8(∗) as a surface insertion. Approximately 45mg of IgY were readily obtained from each yolk with high titers of anti-P particle and anti-VP8(∗) antibodies detected by ELISA, Western blot, HBGA blocking (NoV and RV) and neutralization (RV) assays. Reductions of RV replication were observed with viruses treated with the IgY before and after inoculation into cells, suggesting an application of the IgY as both prophylactic and therapeutic treatment. Collectively, our data suggested that the P-VP8(∗) based IgY could serve as a practical approach against both NoV and RV.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Dai YC,Zhang XF,Tan M,Huang P,Lei W,Fang H,Zhong W,Jiang Xdoi
10.1016/j.antiviral.2012.12.011subject
Has Abstractpub_date
2013-03-01 00:00:00pages
293-300issue
3eissn
0166-3542issn
1872-9096pii
S0166-3542(12)00291-4journal_volume
97pub_type
杂志文章abstract：:The 21st International Conference on Antiviral Research provided novel insights and approaches to drug discovery across a wide array of virologic fields. Topics ranged from the chemical synthesis of new compounds against the human immunodeficiency virus (HIV) to the long-term use of established drugs against influenza...
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abstract：:Yellow fever virus (YFV), a member of the genus Flavivirus, is a mosquito-borne virus found in tropical regions of Africa and South America that causes severe hepatic disease and death in humans. Despite the availability of effective vaccines, YFV is responsible for an estimated 200,000 cases and 30,000 deaths annuall...
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journal_title：Antiviral research
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更新日期：2013-12-01 00:00:00
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