Abstract：
:Buffalopox virus, a zoonotic Indian vaccinia-like virus, is responsible for contagious disease affecting mainly buffaloes, cattle and humans. H3L gene, encoding for an immunodominant major envelope protein of intracellular mature virion of orthopoxviruses, is highly conserved and found to elicit neutralizing antibodies. Therefore in the present study, the immunogenicity and protective efficacy of the recombinant H3L protein of buffalopox virus in laboratory animal models has been evaluated. A partial H3L gene encoding for the C-terminal truncated ectodomain of H3L protein (1M to I280) of BPXV-Vij/96 strain was cloned, over-expressed and purified as histidine-tagged fusion protein (50 kDa) from Escherichia coli using Ni-NTA affinity chromatography. The purified rH3L protein was further used for active immunization of guinea pig (250 μg/dose) and adult mice (10 μg and 50 μg/dose) with or without adjuvants (alum, Freund's Complete Adjuvant and CpG). Subsequently, a gradual increase in antigen specific serum IgG as well as neutralizing antibody titres measured by using indirect-ELISA and serum neutralization test respectively, was noted in both guinea pigs and mouse models. Suckling mice immunized passively with anti-H3L serum showed 80% pre-exposure prophylaxis upon challenge with virulent buffalopox virus strain. An indirect-ELISA based on rH3L protein showed no cross-reactivity with hyperimmune sera against sheeppox virus (SPPV), goatpox virus (GTPV), orf virus (ORFV), foot- and- mouth disease virus (FMDV), peste des petits ruminants virus (PPRV) and bluetongue virus (BTV) during the course of study. The study highlights the potential utility of rH3L protein as a safer prophylactic and diagnostic reagent for buffalopox.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Kumar A,Yogisharadhya R,Venkatesan G,Bhanuprakash V,Shivachandra SBdoi
10.1016/j.antiviral.2015.12.004subject
Has Abstractpub_date
2016-02-01 00:00:00pages
108-16eissn
0166-3542issn
1872-9096pii
S0166-3542(15)30043-7journal_volume
126pub_type
杂志文章abstract：:Lentiviral vectors have been shown to be good candidates for gene transfer protocols; however, prevention of insertional mutagenesis remains problematic. Here we report on the design of a conditionally replicating integrase (IN)-defective lentiviral-hybrid episomal vector in which the insertion of the SV40 promoter/or...
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更新日期：1996-01-01 00:00:00
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更新日期：2014-05-01 00:00:00
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更新日期：2002-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0166-3542(98)00006-0
更新日期：1998-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,meta分析
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更新日期：2011-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.08.006
更新日期：2007-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：1991-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.antiviral.2009.09.010
更新日期：2009-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.09.021
更新日期：2013-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0166-3542(93)90091-v
更新日期：1993-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.antiviral.2007.06.001
更新日期：2007-10-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.11.014
更新日期：2014-02-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2004.05.005
更新日期：2004-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.07.009
更新日期：2018-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0166-3542(97)00039-9
更新日期：1997-09-01 00:00:00
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pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.antiviral.2009.11.004
更新日期：2010-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.antiviral.2011.01.003
更新日期：2011-03-01 00:00:00
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doi：10.1016/j.antiviral.2006.12.004
更新日期：2007-04-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2016-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.08.024
更新日期：2013-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：1999-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
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更新日期：2012-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.antiviral.2008.05.012
更新日期：2008-11-01 00:00:00
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journal_title：Antiviral research
pub_type： 临床试验,杂志文章
doi：10.1016/s0166-3542(00)00110-8
更新日期：2000-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0166-3542(94)90099-x
更新日期：1994-10-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2014.09.008
更新日期：2014-11-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2013.06.011
更新日期：2013-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 临床试验,杂志文章
doi：10.1016/j.antiviral.2014.06.020
更新日期：2014-09-01 00:00:00
abstract：:To identify monoclonal antibodies (mAbs) with high potency and novel recognition sites, more than 25,000 of mouse hybridomas were screened and 4 novel anti-human CCR5 mAbs ROAb12, ROAb13, ROAb14, and ROAb18 showing potent activity in cell-cell fusion (CCF) assay were identified. These mAbs demonstrated potent antivira...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.11.003
更新日期：2007-05-01 00:00:00
