Abstract：
OBJECTIVE:MicroRNAs (miRNAs) are recognised as important regulators of a variety of fundamental biologic processes. Our study was undertaken to examine the role of MicroRNA-203 (miR-203) in modulating nitric oxide (NO) expression in female Sprague-Dawley rat mandibular condylar chondrocytes (MCCs) via targeting transient receptor potential vanilloid 4 (TRPV4) and to demonstrate the possible mechanism of NO inhibition by chondroprotective factor 17β-oestradiol (E2). METHODS:The expression of TRPV4 in mandibular condylar cartilage tissue and MCCs was detected by immunohistochemistry, immunofluorescence (IF), RT-PCR and Western blot, respectively. Primary SD rat MCCs were exposed to lipopolysaccharide (LPS), plus Ruthenium Red, 4α-phorbol 12,13-didecanoate (4αPDD), over-expressed miR-203 or E2 (10(-9) to 10(-6)M), the cellular supernatants were used for NO assay, miR-203 levels were measured by quantitative RT-PCR while TRPV4 expression changes were analysed by Western blot. The dual luciferase activity assay was performed to identify the target gene of miR-203. RESULTS:TRPV4 and miR-203 were stably expressed in MCCs. The MCCs' expression of NO evoked by LPS could be enhanced or depressed by Ruthenium Red or 4αPDD. The dual luciferase assay suggested that TRPV4 was the direct target gene of miR-203. Over-expression of miR-203 inhibited the expression of TRPV4 and increased NO expression in MCCs. E2 inhibited NO expression by inhibition of miR-203, which was concurrent with the up-regulation of TRPV4 expression level in MCCs. CONCLUSION:Our findings first suggested that miR-203 could up-regulate NO expression in female rat MCCs via targeting TRPV4. Moreover, the inhibition of NO by E2 might be at least in part through this mechanism.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Hu F,Zhu W,Wang Ldoi
10.1016/j.archoralbio.2012.08.013subject
Has Abstractpub_date
2013-02-01 00:00:00pages
192-9issue
2eissn
0003-9969issn
1879-1506pii
S0003-9969(12)00291-9journal_volume
58pub_type
杂志文章abstract：:The adherence of streptococci to hydroxyapatite spheroids (HAS) depended upon the strain used, the fermentable carbon source available during bacterial growth, the number of times a clinical isolate had been subcultured on laboratory media and the pre-treatment of the HAS. Sucrose greatly stimulated the secondary colo...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90107-3
更新日期：1983-01-01 00:00:00
abstract：:The bioconcentration of lipophilic organochlorines in ovine dentine in comparison to adipose tissue was examined. Sheep were given a single dose (0.2-1.4 mg/kg body wt) of individual polychlorobiphenyl (PCB) congeners (tetrachlorobiphenyls IUPAC Nos. 54 and 80, and hexachlorobiphenyls IUPAC Nos. 155 and 169) and organ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00079-6
更新日期：2001-12-01 00:00:00
abstract：OBJECTIVE:The present study was designed to investigate (1) whether the non-human primate would be an appropriate animal model for the study of spontaneous periodontitis and its association with metabolic syndrome (MetS), and (2) whether microRNAs (miRNAs) play roles in the co-development of metabolic disorders and per...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.01.004
更新日期：2014-04-01 00:00:00
abstract：:Periodontal mechanosensitive (PM) primary afferent neurones were recorded from the rat trigeminal ganglion and their response to mechanical tooth stimulation studied. The majority (95%) of PM afferents were single-tooth units and most were sensitive to mechanical stimulation of the incisor. They had a sustained respon...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90167-8
更新日期：1994-05-01 00:00:00
abstract：:The cartilage of the mandibular condyle of growing mice was used to monitor changes in the content of protein, DNA, hydroxyproline, hydroxylysine, uronic acid, calcium and phosphate. Protein and DNA increased in a stepwise fashion with one spurt seen during the first 2 weeks of post-natal life a second immediately aft...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90036-5
更新日期：1983-01-01 00:00:00
abstract：BACKGROUND:External apical root resorption is a pathologic consequence of orthodontic tooth movement. Cementum and dentin are removed from the root surface while active force is present. OBJECTIVE:The aim of this study was to identify and quantify extracellular matrix proteins, dentin matrix protein 1 (DMP1), dentin p...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.08.018
更新日期：2007-03-01 00:00:00
abstract：:The association of wheat germ agglutinin (WGA) and peanut agglutinin (PNA) with oral tissues after eating raw wheat-germ or raw peanuts, respectively, was determined. An indirect enzyme-linked immunoassay (ELIA) was used to detect WGA and PNA on epithelial cells. Buccal and tongue epithelial cells, and preparations of...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90002-x
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVE:Interleukin (IL)-12 is an important cytokine for innate and adaptive immunity. We previously reported that IL-12 inhibits tumour necrosis factor (TNF)-α-mediated osteoclast formation by inducing apoptosis. We also reported that TNF-α plays an important role in mechanical loading-induced osteoclast formation a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.07.006
更新日期：2012-01-01 00:00:00
abstract：:Gingival crevicular fluid (GCF) was collected into capillary tubes from healthy gingiva and sites of advanced periodontitis. Following digestion with Pronase E, the glycosaminoglycans were isolated by successive precipitation into 5% cetylpyridinium chloride and 95% ethanol. Unsaturated disaccharide isomers of chondro...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00038-q
更新日期：1995-08-01 00:00:00
abstract：:Actinomyces species are predominant early colonizers of the oral cavity and prime mediators of inter-bacterial adhesion and coaggregation. Previous workers have evaluated the adhesion of Actinomyces spp. by quantitative assessment of sessile, as opposed to planktonic cells attached to substrates, but did not quantify ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.04.003
更新日期：2004-09-01 00:00:00
abstract：:Dehydration by alcohols and concentrated solutions of highly soluble salts caused refractive changes in enamel which were enhanced in the cervical regions. Electron microscopy showed crystals in cervical enamel to vary from normal orientation, size and habit to marked disorientation and reduced size. Consequent increa...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(81)90106-0
更新日期：1981-01-01 00:00:00
abstract：:Normal keratinocytes from epidermis and from buccal mucosa underwent dissimilar stages of differentiation in the same culture medium and responded differently to changes in the composition of the medium. Manifestations of these variations were examined in terms of the expression at the mRNA level (as measured by rever...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00046-r
更新日期：1995-09-01 00:00:00
abstract：:The zinc and strontium content of enamel and dentine from humans aged 9-64 yr was analysed, after systematic sampling, by X-ray fluorescence. Relatively high levels of zinc were found in both hard tissues but there was no significant increase with age. Zinc content decreased significantly from outer to inner enamel an...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90012-5
更新日期：1989-01-01 00:00:00
abstract：:Evidence was sought for urea-induced pH gradients in dental plaque microcosm biofilms cultured from the mixed salivary bacteria in a multi plaque 'artificial mouth'. Application of 500 mmol/l urea for short periods (6 min) to 5-8 mm maximum-thickness plaques induced intraplaque pH gradients of up to 0.7 pH units with ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90147-3
更新日期：1994-06-01 00:00:00
abstract：:The EMG patterns of temporalis, masseter and digastric muscles of twenty Wistar white rats were studied as they ate large and small standard food pellets, bread and pudding. Bipolar EMG electrodes were placed in the muscles and led subcutaneously to a connector pedestal on the rat's head. Integrated records of the EMG...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90090-0
更新日期：1983-01-01 00:00:00
abstract：BACKGROUND:Spondias mombin has been used in folk medicine to treat inflammation in the oral cavity. This study aimed to evaluate the antioxidant and anti-inflammatory activity of S. mombin extract in an oral mucositis experimental model. METHODS:Male hamsters were orally pre-treated with hydroethanolic extract of S. m...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104664
更新日期：2020-03-01 00:00:00
abstract：:These mechanoreceptors were studied with particular regard to their type and the distribution. In both species they were concentrated in the crests of transverse palatine rugae. In the mouse, Meissner corpuscles, glomerular corpuscles, and Merkel cell nerve endings were seen in every palatine ruga, though the first an...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90014-2
更新日期：1990-01-01 00:00:00
abstract：:The expression and distribution of inducible nitric oxide synthase (iNOS) was examined in 12 samples of human temporomandibular joint (TMJ) with internal derangement (ID) and four control specimens. In the diseased joints, strong or definite iNOS reactivity was expressed in synovial lining and endothelial cells; weake...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(00)00086-8
更新日期：2001-01-01 00:00:00
abstract：:A variable capacitance displacement transducer, previously employed for continuously monitoring movements of the rabbit mandibular incisor, was modified for use with the ferret mandibular canine. Comparing this tooth of limited growth with the tooth of continuous growth, similarities were seen in the reactions to extr...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90177-2
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVES:A resin infiltrant was employed for the treatment of active white spot lesions due to its ability to penetrate into the enamel pores and prevent the progression of the lesion. However, limited information is available about its mechanical effect on different artificial enamel lesions as well as on its resist...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.07.011
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVE:To investigate the association between passive smoking, as determined by salivary cotinine level, and caries, salivary pH, buffering capacity, flow rate and microbiological counts in young children. DESIGN:Ninety passive smokers (PS) with a mean age of 5.02 years and 90 healthy age-matched controls were incl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.05.007
更新日期：2008-10-01 00:00:00
abstract：:Calcium and protein kinase C may be directly involved in exocytosis. However, in the rat submandibular gland, cAMP-mediated events appear to be required for mucin secretion. Calcium may be involved, but a direct signal-transduction role for calcium and protein kinase C in regulating such secretion has yet to be establ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90075-7
更新日期：1989-01-01 00:00:00
abstract：OBJECTIVE:The biofilm of Streptococcus mutans is associated with induction of dental caries. Also, they produce glucan as an extracellular polysaccharide through glucosyltransferases and help the formation of cariogenic biofilm. β-caryophyllene has been used for therapeutic agent in traditional medicine and has antimic...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.01.009
更新日期：2018-04-01 00:00:00
abstract：OBJECTIVES:to investigate p16(INK4a) and p14(ARF) tumor suppressor gene methylation status, determine telomere length and assess the importance of these epigenetic and genetic parameters in the development of pleomorphic adenoma and carcinoma ex pleomorphic adenoma of the parotid salivary glands. MATERIALS AND METHODS...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.08.011
更新日期：2015-11-01 00:00:00
abstract：:Although most cases of cleft lip and palate are free of other developmental defects, children with isolated cleft lip/palate are at enhanced risk of delayed growth and reduced final size. Three variables were assessed in the permanent dentition away from the cleft site: congenital absence by tooth type (which ranged f...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90210-2
更新日期：1990-01-01 00:00:00
abstract：:The secretions (HPS) contained an arylamidase-like enzyme discovered by chromatography on Sephadex G-100 Superfine columns using N-L-alanyl-2-naphthylamine (2NA) as substrate. The enzyme was fractionated in the void volume, suggesting that its molecular weight was 150,000 or higher. It hydrolysed, with decreasing rate...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90050-0
更新日期：1985-01-01 00:00:00
abstract：:With scanning electron microscopy, globules were seen in inter-cellular spaces between ameloblasts with ruffled borders and papillary cells at early maturation stage. Numerous globules were located on longitudinal folds between parallel ridges on the sides of ameloblasts, but only a few in the papillary layer. In prev...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90121-3
更新日期：1984-01-01 00:00:00
abstract：:The localization of cathepsin L and rat salivary cystatin-3 (RSC-3) in rat osteoclasts (rat femoral and alveolar bones) treated with or without E-64 (control) was examined immunocytochemically. In osteoclasts pretreated with E-64, immunoreactivity for cathepsin L was very weak extracellularly compared to that in the c...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00003-4
更新日期：1997-04-01 00:00:00
abstract：:The acquired enamel pellicle formed by selective adsorption of proteins in whole saliva is a protective integument on the tooth surface. The purpose of the present study was to investigate the formation of human acquired enamel pellicle using an in vitro hydroxyapatite (HA) model and 3H-histatin 5 to allow accurate me...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.06.003
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVE:To explore the gene mutation in an incontinentia pigmenti (IP) patient with syndromic tooth agenesis. METHODS:Long-range polymerase chain reaction (PCR) and Sanger sequencing were used to detect inhibitor of nuclear factor kappa-B kinase subunit gamma (IKBKG) mutation in the IP patient. We used the nuclear f...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.03.013
更新日期：2019-05-01 00:00:00
