Abstract：
:As shown previously doxorubicin (1 μM) plus sulindac (50 μM) reduced the expression of ABCB1 (ATP-binding cassette, sub-family B (MDR/TAP), member 1) mRNA in HeLa cells and this effect was accompanied by increased apoptosis. The aim of this study was to define if the decrease of ABCB1 expression or blocking of P-glycoprotein (P-gp) can affect the expression of the apoptotic genes determined with use of quantitative real time polymerase chain reaction (qRT-PCR). Western blot was used for visualization of chosen pro- and antiapoptotic proteins. Doxorubicin was the main compound which affected the apoptotic genes. The effectiveness of the drugs in reducing of P-gp function has been shown as not being related to the regulation of apoptotic gene transcription. In this experimental scheme, regulation of apoptotic gene transcription depended on the kind of P-gp modulator.
journal_name
Cancer Genomics Proteomicsjournal_title
Cancer genomics & proteomicsauthors
Gruber BM,Krzysztoń-Russjan J,Bubko I,Koronkiewicz M,Anuszewska ELsubject
Has Abstractpub_date
2012-05-01 00:00:00pages
153-61issue
3eissn
1109-6535issn
1790-6245pii
9/3/153journal_volume
9pub_type
杂志文章abstract：BACKGROUND:The transcription factor SOX2 controls gene expression during development and has roles in both neurogenesis and gliogenesis. MATERIALS AND METHODS:The current study utilized immunohistochemistry, immunofluorescence, molecular genetics (gene copy number analysis) and Western blotting to examine SOX2 express...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2011-05-01 00:00:00
abstract：:Human telomerase reverse transcriptase (hTERT) is overexpressed in prostate cancer. Estrogen plays a central role in the development of prostate cancer. hTERT activity has been shown to be increased after estrogen treatment. Although significant efforts have been made to understand the role of estrogen, the telomerase...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2009-11-01 00:00:00
abstract：:Previous studies have suggested that galectin-3 expression was markedly elevated in papillary thyroid carcinoma compared to other thyroid diseases. In order to better understand this protein, galectin-3 from papillary thyroid carcinoma was partially purified by affinity chromatography on lactose-agarose. Proteins elut...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：BACKGROUND:Cowden syndrome (CS) is a rare autosomal-dominant inherited disorder characterized by multiple hamartomas. While the hamartomas are benign, patients with CS have increased risk of osteosarcoma and of breast, thyroid, endometrial, soft-tissue and colonic neoplasms. Germline mutations of phosphatase and tensin...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20069
更新日期：2018-03-01 00:00:00
abstract：:Backgound/Aim: Malignant mesothelioma (MM) is an aggressive and fatal pleural cancer. The cell secretome offers information allowing insight into the pathogenesis of MM while offering the possibility to identify potential therapeutic targets and biomarkers. In the present study the secretome protein profile of MM cell...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20023
更新日期：2017-03-01 00:00:00
abstract：BACKGROUND:Few studies have analyzed the association between human telomerase reverse transcriptase (hTERT) protein expression (nuclear and cytoplasmic localization), hTERT methylation status, and human papillomavirus (HPV) genotype infection in cervical cancer. PATIENTS AND METHODS:One hundred seventy-three patients ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20218
更新日期：2020-09-01 00:00:00
abstract：:After the completion of sequencing for dozens of genomes, as well as the draft of human genome, a major challenge is to characterize genome-wide transcriptional regulation networks. Identification of regulatory functions for transcription factor binding sites in eukaryotes greatly enhances our understanding of the net...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:Oncogenic infection by HPV, eventually leads to cervical carcinogenesis, associated by deregulation of specific pathways and protein expression at the intracellular and secretome level. Thus, secretome analysis can elucidate the biological mechanisms contributing to cervical cancer. In the present study we s...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：10.21873/cgp.20060
更新日期：2017-11-01 00:00:00
abstract：BACKGROUND:Trastuzumab is a monoclonal antibody against HER2-positive breast cancer. Despite improving the natural history of the disease, there is a number of patients who are resistant to it, whereas all patients will eventually develop resistance and disease will progress. Inconsistent preclinical data show that the...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20105
更新日期：2018-11-01 00:00:00
abstract：UNLABELLED:A growing body of evidence suggests that early growth response-1 (Egr-1), a transcription factor, may function as a tumor suppressor. The aim of this study was to gain more evidence to support the role of Egr-1 in the suppression of cancer cell growth and to examine the potential correlation between Egr-1 an...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2007-11-01 00:00:00
abstract：BACKGROUND/AIM:The outcome of patients with advanced hepatocellular carcinoma (HCC) remains poor and therapeutic options, including sorafenib, the first anti-cancer drug proved to prolong survival in patients with advanced HCC, are limited. However, no clinically useful predictive biomarker for sorafenib has been repor...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20159
更新日期：2019-11-01 00:00:00
abstract：AIM:To identify and study targets of microRNA biomarkers of glioblastoma survival across events (death and recurrence) and phases (life expectancy or post-diagnostic) using functional and network analyses. MATERIALS AND METHODS:microRNAs associated with glioblastoma survival within and across race, gender, recurrence,...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
abstract：:The concept of individualized cancer chemotherapy emerged three decades ago from the observation that a small fraction of cells in primary tumors can form colonies in soft agar similar to stem cells of the hematopoietic system. In a series of retrospective and prospective clinical studies, clonogenic tumor growth and ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2007-05-01 00:00:00
abstract：:Testicular germ cell tumors (GCT) are the most frequent malignancy in young adults and arise from intratubular germ cell neoplasia undetermined (IGCNU, also referred to as carcinoma in situ, CIS). To determine the transcriptional programs involved in the transition from normal germ cells to GCT, and to further elucida...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：BACKGROUND/AIM:The altered cellular repair capacity plays a critical role in genomic instability and carcinogenesis. We aimed at evaluating the contribution of the polymorphic variant in apurinic/apyriminidinic endonuclease (APEX1) gene to its mRNA and protein levels and the risk of endometriosis. PATIENTS AND METHODS...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2014-11-01 00:00:00
abstract：:The expression profiles of the erythropoietin producing hepatocellular carcinoma (Eph) receptor family of tyrosine kinases have been previously shown to provide molecular signatures of normal breast cells, breast tumor cells and invasive breast carcinoma cells. In particular, the expression of EphB6 receptor is lost i...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：BACKGROUND:Fusion genes driving tumourigenesis have drawn the attention of researchers and oncologists. Despite the importance of such molecular alterations, there are no comprehensive reproducible methods for detecting fusion genes. MATERIALS AND METHODS:Nineteen paediatric brain tumours of five types, namely pilocyt...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20093
更新日期：2018-07-01 00:00:00
abstract：:We recently reported that functional loss of p53 altered the responses of human HCT116 colon cancer cells to apoptosis triggers. To examine the molecular basis underlying the differential responses to drug treatment in the cancer cells, we performed a proteomic analysis in order to compare the protein expressions betw...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:Patients with pancreatic ductal adenocarcinoma have a dismall prognosis because at the time of diagnosis, in the vast majority of patients the tumor has already disseminated to distant organs and the therapeutic benefit of approved agents such as gemcitabine is limited. Therefore, the identification and preclinical an...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：10.21873/cgp.20149
更新日期：2019-11-01 00:00:00
abstract：:Although the statistical probability of therapeutic success is known for larger groups of cancer patients, the clinical response to chemotherapy of the individual patient remains uncertain. It would be of great value to know whether or not an individual tumor responds to the proposed therapy. The concept of sensitivit...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2004-03-01 00:00:00
abstract：:Although several microscopic techniques are available for separation of small groups of cells from surrounding tissue, the high energy concentrated into a small area, the easy control of beam position and the lack of direct contact with the material to be dissected make lasers the best option for a large scale microdi...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2006-05-01 00:00:00
abstract：BACKGROUND:Pancreatic cancer, has a very high mortality rate and requires novel molecular targets for diagnosis and therapy. Genetic association studies over databases offer an attractive starting point for gene discovery. MATERIALS AND METHODS:The National Center for Biotechnology Information (NCBI) Phenome Genome In...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：AIM:This pilot study assessed the association of BIM deletion polymorphism and BIM RNA isoform in patients with EGFR-positive non-small cell lung cancer (NSCLC). PATIENTS AND METHODS:The study included 33 patients with EGFR-positive NSCLC treated with gefitinib. BIM deletion polymorphism and BIM RNA isoform (EL/L/S/γ)...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20010
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:Salivary gland cancer is a common type of head and neck cancer characterized by occasional deep invasion and lung metastasis. The precise role of sex steroid hormones in salivary gland cancer is unclear. To address this issue, we investigated whether the estrogen axis modulates salivary adenocarcinoma (SAC) ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：BACKGROUND:Identification and characterization of genes that are relevant to pancreatic cancer remains a priority for developing detection and diagnostic tests and identifying targets for treatment. MATERIALS AND METHODS:In order to discover relevant genes, we developed a microarray composed of 5763 pancreas and pancr...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
abstract：:Lung cancer is the leading cause of cancer-related to death in both men and women. Protein biomarkers for lung cancer were investigated using the expression of proteins from lung cancer cell line (A549) and compared with those of normal lung fibroblast cell line (MRC-5). Two-dimensional gel electrophoresis of A549 and...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2009-07-01 00:00:00
abstract：BACKGROUND:Patients with metastazing malignant melanoma have a poor outcome and determination of thickness of the primary tumor remains as the most important prognostic predictor. The aim of this study was to use an antibody-based proteomics strategy to search for new molecular markers associated with melanoma progress...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2008-11-01 00:00:00
abstract：BACKGROUND/AIM:Medulloblastoma (MBL), an archetypal primitive neuroectodermal tumor of the cerebellum, is the most common pediatric central nervous system malignancy representing approximately 20% of all childhood brain tumors. Herein, we report on a new xenotransplantable tumor cell line, derived from a 6-year-old fem...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20037
更新日期：2017-07-01 00:00:00
abstract：:TACC2 is a member of the transforming acidic coiled-coil-containing protein family and is associated with the centrosome-spindle apparatus during cell cycling. The TACC2 gene is expressed in various splice forms predominantly in postmitotic tissues, including heart, muscle, kidney, and brain. Recent work has shown tha...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND/AIM:Epithelioid osteoblastoma is a rare benign tumor of the bone. Its pathogenesis is unknown and little is known regarding its genetic features. MATERIALS AND METHODS:Cytogenetic, RNA sequencing, reverse transcription polymerase chain reaction (RT-PCR), genomic PCR, and Sanger sequencing analyses were perf...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20141
更新日期：2019-09-01 00:00:00
