Abstract：
BACKGROUND:Proteins involved in the aberrant regulation of signaling pathways and their downstream effectors are promising targets for cancer therapy. Survivin is an anti-apoptotic and cell cycle-promoting protein, which is consistently overexpressed in cancer cells. In normal cells, its expression is tightly controlled by signaling pathways and their associated transcriptional activators and repressors. In cancer cells, its expression is enhanced as a consequence of oncogenic signaling. We investigated the potential of a novel, peptide-based survivin inhibitor in breast cancer (SK-BR-3, MDA-MB-468) and glioblastoma (Tu9648) cells. These cells express high levels of survivin. MATERIALS AND METHODS:We downregulated survivin expression in tumor cells with a lentiviral gene transfer vector encoding a specific shRNA and a recombinant fusion protein, rSip, comprising the FTH1-derived survivin interaction domain, the human thioredoxin and a protein transduction domain. RESULTS:Downregulation of survivin expression decreased the growth and viability of tumor cells in culture and reduced growth of the cancer cells upon transplantation into immunodeficient mice. rSip selectively targets the anti-apoptotic function of survivin and causes tumor cell death. Non-transformed NIH/3T3 and MCF10A cells remain unaffected. CONCLUSIONS:rSip provides a lead structure for the development of drugs targeting the tumor cell "addiction protein" survivin.
journal_name
J Cancer Res Clin Oncoljournal_title
Journal of cancer research and clinical oncologyauthors
Weiss A,Brill B,Borghouts C,Delis N,Mack L,Groner Bdoi
10.1007/s00432-012-1195-1subject
Has Abstractpub_date
2012-07-01 00:00:00pages
1205-20issue
7eissn
0171-5216issn
1432-1335journal_volume
138pub_type
杂志文章abstract：PURPOSE:Metastasizing epithelioid sarcoma (ES) is an extremely aggressive tumor, because conventional chemotherapy and irradiation are largely ineffective. Here, we analyzed the impact of the CD95-mediated drug-induced apoptosis in ES cell lines. METHODS:The effects of paclitaxel (Taxol) and 5-FU were determined by MT...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-007-0340-8
更新日期：2008-06-01 00:00:00
abstract：PURPOSE:Triple (ER-, PR-, HER2-) negative breast carcinoma lack targeted therapies, making this group of tumors difficult to treat. By definition, the lack of HER2 expression means a case scoring 0 or 1+ after immunophenotypical analysis and makes the patients avoiding therapeutical chances with anti-HER2 inhibitors. W...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-013-1479-0
更新日期：2013-09-01 00:00:00
abstract：PURPOSE:A disintegrin and metalloprotease with motif 5(ADAMTS5) has been involved in colorectal cancer (CRC) with hypermethylation in the promoter. However, its role in CRC remains unclear. The aim of this study was to explore the clinical significance and biological effect of ADAMTS5 on colorectal carcinogenesis. Thro...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-017-2545-9
更新日期：2018-02-01 00:00:00
abstract：PURPOSE:Human papillomavirus (HPV) type 16 and 18 are the most prevalent genotypes in cervical cancers. The viral oncoproteins E6 and E7 are considered to be tumor-specific targets for immunotherapy. HPV E7 antigen-loaded dendritic cells (DC) were evaluated as cellular tumor vaccine. METHODS:Autologous monocyte-derive...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-003-0462-6
更新日期：2003-09-01 00:00:00
abstract：PURPOSE:To study the effects of surgery and timing of radiotherapy on patient survival in grade 2 gliomas. METHODS:One hundred and eighty-nine patients with diffuse astrocytomas, oligoastrocytomas, and oligodendrogliomas, World Health Organization grade 2, treated between 1982 and 2000 were identified. The impact of t...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-003-0423-0
更新日期：2003-03-01 00:00:00
abstract：:Antiproliferative effects of free retinoic acid (RA) and liposome-encapsulated RA (RAlp) were compared in a squamous carcinoma system using both monolayer cells and multicellular tumor spheroids (MTS), an in-vivo-like model with three-dimensional histological structure. Initial studies examined the effect of lipid com...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01225262
更新日期：1992-01-01 00:00:00
abstract：PURPOSE:The aim of this study is to investigate whether GDF3 is related to the progression of human breast cancer and the effects of GDF3 on breast cancer cells. METHODS:The expression of GDF3 in 24 breast cancer specimens paired with corresponding neighboring nontumorous tissue was studied by Western blot. Breast can...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-012-1213-3
更新日期：2012-06-01 00:00:00
abstract：PURPOSE:Cancer incidence in the elderly population has been continuously rising, and their treatment is an increasing concern among oncologists. This study aimed to evaluate the incidence and risk factors for morbidity and mortality after major oncological surgery in elderly patients with head and neck squamous cell ca...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-016-2141-4
更新日期：2016-06-01 00:00:00
abstract：INTRODUCTION:Non-adherence to anti-hormonal therapy is a major problem in gynecologic oncology. Reasons reported are side effects and lack of support. The aim of our study was an analysis of influence of experiences of patients with endocrine therapy and communication and information on this topic and their influence o...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-014-1779-z
更新日期：2015-01-01 00:00:00
abstract：:This article summarizes the various current uses of cytokines, and bone marrow and peripheral blood stem cell transplantation for cancer treatment. Interferons, interleukin-2 and hematopoietic growth factors are extensively used for the treatment of hematopoietic as well as nonhematopoietic malignancies, either as the...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,评审
doi：10.1007/BF01213315
更新日期：1995-01-01 00:00:00
abstract：PURPOSE:Our goal was to prospectively evaluate self-reported quality-of-life (QoL) during second-line therapy in 51 consecutive patients with cytokine-refractory kidney cancer treated with sorafenib or sunitinib. METHODS:QoL was assessed by the EORTC QoL questionnaire QLQ-C30 at baseline and at weeks 4, 6, 10, 12 and ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-008-0438-7
更新日期：2009-01-01 00:00:00
abstract：PURPOSE:The TNM classification is used to assess cancers of the oral cavity, and advancements in imaging techniques have revealed clear variations in tumor volume at presentation. This study therefore aimed to clarify whether preoperative imaging, with exact measurements of the tumor, could affect post-surgery survival...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-014-1881-2
更新日期：2015-06-01 00:00:00
abstract：:Recently a new proto-oncogene, the murine double-minute 2 (MDM2), has been described. MDM2 becomes oncogenic due to amplification and overexpression. Among other proto-oncogenes MDM2 becomes interesting since MDM2 protein can associate with both mutant and wild type p53 tumor suppressor gene products and thus inhibit ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01372566
更新日期：1994-01-01 00:00:00
abstract：PURPOSE:Metastasis is a primary cause of colorectal cancer (CRC)-related death, and cancer stem cells (CSCs) are thought to be majorly responsible for initiating metastatic behaviors. Doublecortin-like kinase 1 (DCLK1) was recently discovered to be a marker for gastrointestinal CSCs. Here, we aimed to explore whether D...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-016-2218-0
更新日期：2016-10-01 00:00:00
abstract：:A series of 202 breast cancer biopsy specimens were analysed immunohistochemically for collagen IV to demonstrate basement membrane (BM) structures and blood vessels within tumour tissue. Integrity of the BM was graded into four categories and the number of vascular channels per square millimetre of tumour tissue were...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01245375
更新日期：1994-01-01 00:00:00
abstract：:In the supernatant of melanoma cell culture SK mel 25 three indolic compounds - 5-hydroxy-6-methoxyindole, 5-hydroxy-6-methoxyindolyl-2-carboxylic, and 6-hydroxy-5-methoxyindolyl-2-carboxylic acids - have been identified. The supernatants were extracted with ethyl acetate, derivatized, and analyzed by gas chromatograp...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00395758
更新日期：1983-01-01 00:00:00
abstract：PURPOSE:Matrix metalloproteinases MMP-2 and MMP-9 are implicated in invasion and metastasis of malignant tumors. We investigated the expression and activation of MMP-2 and MMP-9 in lung cancer compared with normal lung parenchyma, and looked for a potential marker of malignancy. METHODS:Thirty-six pulmonary carcinomas...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-001-0320-3
更新日期：2002-04-01 00:00:00
abstract：BACKGROUND:In the phase 3 BFORE trial (NCT02130557), treatment with bosutinib resulted in a significantly higher major molecular response rate at 12 months versus imatinib in the modified intent-to-treat (mITT) population of patients with newly diagnosed chronic phase chronic myeloid leukemia (CP CML). Assessment of pa...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00432-019-02894-3
更新日期：2019-06-01 00:00:00
abstract：PURPOSE:We have demonstrated that overexpression of osteopontin (OPN) could contribute to metastasis in hepatocellular carcinoma (HCC), and that OPN-positive cancer cells are often localized in the periphery of cancer nodules adjacent to stromal cells. This study was to identify the difference of intratumor genomic abe...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-009-0695-0
更新日期：2010-04-01 00:00:00
abstract：:The chemosensitizing potency of dexniguldipine hydrochloride (B8509-035) on epidoxorubicin was assessed in a multidrug-resistant (MDR) tumour model, the intrinsic MDR rat colon carcinoma CC531. In vitro in the sulphorhodamine B cell-viability assay the cytotoxicity of epidoxorubicin was increased approximately 15-fold...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01212879
更新日期：1996-01-01 00:00:00
abstract：:Chemically induced (autochthonous) tumors in the rodent are thought to be the best models at hand to obtain results transferable to the clinical situation. Four different autochthonous tumor models: 1,2-dimethylhydrazine, N-methylnitrosourea (MNU), N-methyl-N-nitro-nitrosoguanidine (MNNG), and N-nitroso-acetoxymethyl-...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00400756
更新日期：1986-01-01 00:00:00
abstract：OBJECTIVE:How could we transfer knowledge about healing plants from other cultures? We analyzed the results of literature search in two exemplary fields-oral mucositis and polyneuropathy. MATERIALS AND METHODS:In both systematic searches of existing literature, we found 57 studies investigating 68 plants from differen...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,评审
doi：10.1007/s00432-020-03415-3
更新日期：2021-02-01 00:00:00
abstract：OBJECTIVE:Renal cell carcinoma (RCC) is the most common malignancy of the urinary system, and it is a serious threat to human health. HOXA transcript at the distal tip (HOTTIP), located at the 5' end of the HOXA locus, is a long non-coding RNA that has been newly discovered in recent years. It has been reported to prom...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-018-2808-0
更新日期：2019-03-01 00:00:00
abstract：:The role of different cytogenetic changes has been extensively evaluated in patients with acute myeloid leukemia (AML), and cytogenetic analysis of AML blasts is essential to form prognostic subgroups in order to stratify for the extent of therapy. Nevertheless, 40-45% of AML patients lack such cytogenetic markers, i....
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,评审
doi：10.1007/s00432-008-0524-x
更新日期：2009-04-01 00:00:00
abstract：PURPOSE:In this study, we applied laser capture microdissection and a proteomic approach to identify novel nasopharyngeal carcinoma (NPC) biomarkers. METHODS:Proteins from pooled microdissected NPC and normal nasopharyngeal epithelial tissues (NNET) were separated by two-dimensional gel electrophoresis, and differenti...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-009-0623-3
更新日期：2009-12-01 00:00:00
abstract：:We performed intraoperative peritoneal cytology in 171 gastric cancer patients undergoing curative surgery. Intraperitoneal free cancer cells were demonstrated in almost all patients in whom the area of serosal cancer invasion exceeded 15-20 cm2. In patients with both serosal cancer invasion and free cancer cells the ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00402474
更新日期：1984-01-01 00:00:00
abstract：OBJECTIVE:To examine the associations between modifiable health-related factors, such as smoking, low physical activity and higher body mass index (BMI), and annual health care visits and expenditures among adult cancer survivors in the United States. METHODS:Using data from the 2010-2014 Medical Expenditures Panel Su...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-017-2494-3
更新日期：2017-12-01 00:00:00
abstract：PURPOSE:This study was to investigate whether the topoisomerase (Top) I inhibitor topotecan and the Top II inhibitor etoposide could modulate the hypoxia-induced HIF-1alpha expression in non-small cell lung cancer (NSCLC) cell lines. METHODS:Hypoxic conditions were maintained in a humidified airtight anaerobic incubat...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-009-0543-2
更新日期：2009-08-01 00:00:00
abstract：PURPOSE:To investigate the effects of Akt/ARK5 pathways on the metastatic potential of human breast cancer cells. MATERIALS AND METHODS:The human ARK5 gene was transfected into MDA-MB-231 cells. Effects of ARK5 on MDA-MB-231 cells were investigated in vitro. The tumorigenicity and spontaneously metastatic capability r...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-011-1102-1
更新日期：2012-02-01 00:00:00
abstract：:This study was undertaken in order to compare the usefulness of three indices of tumour proliferation in detecting primary hepatocellular carcinoma (HCC) and in differentiating this neoplasm from liver cirrhosis. In 10 patients with HCC and in 63 with liver cirrhosis serum alpha-fetoprotein (AFP), tissue polypeptide a...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00391702
更新日期：1989-01-01 00:00:00
