Abstract：
PURPOSE:Aging is believed to affect epigenetic marking of brain DNA with 5-methylcytosine (5mC) and possibly via the 5mC to 5-hydroxymethylcytosine (5hmC) conversion by TET (ten-eleven translocation) enzymes. We investigated the impact of aging on hippocampal DNA 5-hydroxymethylation including in the sequence of aging-susceptible 5-lipoxygenase (5-LOX) gene. METHODS:Hippocampal samples were obtained from C57BL6 mice. Cellular 5hmC localization was determined by immunofluorescence. The global 5mC and 5hmC contents were measured with the corresponding ELISA. The 5-LOX 5hmC content was measured using a glucosyltransferase/enzymatic restriction digest assay. TET mRNA was measured using qRT-PCR. RESULTS:Global hippocampal 5hmC content increased during aging as did the 5hmC content in the 5-LOX gene. This occurred without alterations of TET1-3 mRNAs and without changes in the content of 8-hydroxy-2-deoxy-guanosine, a marker of non-enzymatic DNA oxidation. CONCLUSIONS:The aging-associated increase of hippocampal 5hmC content (global and 5-LOX) appears to be unrelated to oxidative stress. It may be driven by an altered activity but not by the increased expression of the three TET enzymes. Global 5hmC content was increased during aging in the absence of 5mC decrease, suggesting that 5hmC could act as an epigenetic marker and not only as an intermediary in DNA demethylation. Further research is needed to elucidate the functional implications of the impact of aging on hippocampal cytosine hydroxymethylation.
journal_name
Restor Neurol Neuroscijournal_title
Restorative neurology and neuroscienceauthors
Chen H,Dzitoyeva S,Manev Hdoi
10.3233/RNN-2012-110223subject
Has Abstractpub_date
2012-01-01 00:00:00pages
237-45issue
3eissn
0922-6028issn
1878-3627pii
G5670V2407151W0Ljournal_volume
30pub_type
杂志文章abstract：:Basic science studies demonstrated mechanisms of plasticity and metaplasticity. More recent human studies identified some of these mechanisms as operating in multiple areas of human cognition, such as learning and memory, and in functional recovery from lesions in the CNS, as in stroke. One of the goals of neurorehabi...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章,评审
doi：
更新日期：2004-01-01 00:00:00
abstract：:Wound-derived trophic factors released by the injured brain are thought to reach a peak 1-2 weeks after injury. It has been proposed that such factors can promote the survival, growth and functional capacity of embryonic tissue grafts. To test the generality of this hypothesis, control rats and rats with aspirative fi...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-1989-1109
更新日期：1989-01-01 00:00:00
abstract：PURPOSE:Current models of intracerebral hematoma are difficult to use for neurotransplantation studies because of high mortality and important variations of morphology, size and location of blood deposits. We propose a modification of the autologous blood infusion technique in rats to reduce these limitations. METHODS...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:In 2009 the German BMBF (German Ministry of Education and Research) established an Integrated Center for Research and Treatment (IFB(LMU)) of Vertigo, Balance and Ocular Motor Disorders in Munich. After the 10-year period of funding by the BMBF, it is envisioned that the (IFB(LMU)) will continue over the long term wit...
journal_title：Restorative neurology and neuroscience
pub_type： 社论
doi：10.3233/RNN-2010-0510
更新日期：2010-01-01 00:00:00
abstract：PURPOSE:There is considerable variability in the extent and nature of the glial response to injury and neurodegeneration. Transplantation of fetal cortical tissue onto the brain of neonatal host rats or mice results in region-specific changes dependent on where the fetal tissue is placed. These changes include chronic ...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-150591
更新日期：2016-01-01 00:00:00
abstract：:While the regenerating capacity of peripheral nerves has been the subject of intensive study, little is known about the regenerative capacity of the autonomic nervous system. Using an animal model, where the pupil diameter of the eye in the rat serves as a parameter of autonomic function, we studied whether systemic t...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-1994-7106
更新日期：1994-01-01 00:00:00
abstract：PURPOSE:Glutamate transporters are responsible for removing glutamate from the extracellular space and have the potential to protect neurons from excitotoxicity. In the present study, the effects of ceftriaxone and (2R, 4R)-APDC (APDC) on the protein expression of GLAST and GLT-1, the rate of glutamate uptake, and neur...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-2011-603
更新日期：2011-01-01 00:00:00
abstract：:Axonotmesis of the left sciatic nerve was performed on adult male Wistar rats. T1 values of the crushed nerve samples were determined with proton magnetic resonance spectroscopy. Denervation changes of the dependent hind plantar flexor muscles were assessed with proton magnetic resonance imaging of their cross-section...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-1992-4601
更新日期：1992-01-01 00:00:00
abstract：PURPOSE:Ischemic stroke is associated with a variety pathophysiological changes affecting both glial and neuronal brain tissue. These changes are mirrored in the release of specific proteins into peripheral blood. Neurone-specific enolase (NSE), protein S100B and glial fibrillary acidic protein (GFAP) are those protein...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
abstract：BACKGROUND:Robotic rehabilitation is a highly promising approach to recover lost functions after stroke or other neurological disorders. Unfortunately, robotic rehabilitation currently suffers from "motor slacking", a phenomenon in which the human motor system reduces muscle activation levels and movement excursions, o...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-180830
更新日期：2018-01-01 00:00:00
abstract：:Over the past few decades astrocytes have emerged from being considered simple packing tissue in the brain to become major players in the development, survival and functioning of central nervous system (CNS) neurons. As the influence that astrocytes (and the various molecules they produce) have on the development of C...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
abstract：PURPOSE:To evaluate the efficacy of an expanded form of Constraint-Induced Movement Therapy (eCIMT) that renders CIMT, originally designed for treating mild-to-moderate upper-extremity hemiparesis, suitable for treating severe hemiparesis. METHODS:Twenty-one adults ≥1 year after stroke with severe upper-extremity hemi...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章,随机对照试验
doi：10.3233/RNN-170792
更新日期：2018-01-01 00:00:00
abstract：PURPOSE:Previous neuroimaging studies of oddball tasks and other paradigms measuring attention processes support right hemisphere dominance for attentional processes. Using an auditory selective attention task, we studied the functional asymmetry of the human brain in response to attended or unattended deviant tones. S...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:Injury of the nervous system initiates a cascade of signal transduction including a rise in extracellular glutamate which leads to subsequent secondary neuronal loss. Excitotoxicity is known to play a crucial role in cell death spinal cord trauma. Gangliosides, particularly monosialogangliosides (GM1), are protective ...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：INTRODUCTION:Traumatic brain injuries (TBI) are one of the most common consequences of traffic accidents. Patients with mild, moderate or severe brain injuries suffer from physical, cognitive, behavioral, emotional and social problems. Most of these problems have been a long standing focus amongst practitioners and res...
journal_title：Restorative neurology and neuroscience
pub_type： 共识发展会议,杂志文章,评审
doi：
更新日期：2002-01-01 00:00:00
abstract：:The lamination of dentate gyrus afferents established during development is maintained following lesion-induced reactive growth in the adult. After partial deafferentation sprouts from undamaged afferents restore most synapses, while respecting the laminae relative boundaries. No evidence of trans-laminar sprouting ha...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
abstract：:Retinal and cortical lesions are completely different events that trigger visual cortical plasticity. We therefore compared the cortical effects of homonymous lesions of the central retina with effects of cortical lesions. All in vivo experiments were performed in anaesthetized, adult cats. Retinal lesions were made w...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:Robot-assisted gait training (RAGT) is widely used in children with cerebral palsy (CP), but information about optimal intervention frequency and duration is still lacking and the current evidence about effect of RAGT on motor endurance and fitness is very preliminary. OBJECTIVE:To investigate the effect of...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-180897
更新日期：2019-01-01 00:00:00
abstract：:The mechanisms underlying recovery of function following damage to the CNS, although suspected, are virtually unknown. After damage to the adult cat spinal cord, recovery of motor behavior depends on which systems have been interrupted and which remain intact. For example, following hemisection, overground (voluntary)...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-1991-245623
更新日期：1991-01-01 00:00:00
abstract：:Many toxic factors are generated during stroke that contribute directly to the death of neurons. Several recent studies suggest that a suicide-like phenomena similar to apoptosis or programmed cell death also contributes to the loss of neurons in stroke. The evidence implicating apoptosis in stroke can be divided into...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-1996-9404
更新日期：1996-01-01 00:00:00
abstract：:Pyramidal neurons inside transplants of embryonic nervous tissue are capable of generating axonal extrinsic hippocampal fiber connections over considerable distances to appropriate target areas in the mature brain. The establishment of long-distance graft efferents to the lateral septum and to the entorhinal cortex wa...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-1991-3103
更新日期：1991-01-01 00:00:00
abstract：:The olfactory nerve differs from cranial nerves III-XII in that it contains a specialised type of glial cell, called 'olfactory ensheathing cell' (OEC), rather than Schwann cells. In addition, functional neurogenesis persists postnatally in the olfactory system, i.e. the primary olfactory pathway continuously rebuilds...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章,评审
doi：
更新日期：2008-01-01 00:00:00
abstract：PURPOSE:The present study was designed to investigate the neural correlates of German Sign Language (Deutsche Gebärdensprache; DGS) processing. In particular, was expected the impact of the visuo-spatial mode in sign language on underlying neural networks compared to the impact of the interpretation of linguistic infor...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：PURPOSE:Repetitive mild traumatic brain injury (TBI) is a major military and sports health concern. The purpose of this study was to determine if a diet rich in omega-3 fatty acids would reduce cognitive deficits and neuronal cell death in a novel fluid percussion rat model of repetitive mild TBIs. METHODS:Thirty-two ...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-130316
更新日期：2013-01-01 00:00:00
abstract：PURPOSE:The aim of the study was to examine if improvements of stimulus detection performance in visual field tests after intensive visual training of the visual field border zone in patients with visual field defects are associated with changes in self-reported vision- and health-related quality of life (QoL). METHOD...
journal_title：Restorative neurology and neuroscience
pub_type： 临床试验,杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：PURPOSE:The quality of presurgical evaluation in focal extratemporal epilepsy surgery is highly dependent on precise structural and functional identification of the epileptic focus. Navigated transcranial magnetic stimulation (nTMS) is a tool that combines the spatial information of high-resolution magnetic resonance i...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-2010-0540
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVE:To report the safety and efficacy of a novel therapeutic trial with umbilical cord blood (UCB) and concomitant recombinant human erythropoietin (rhEPO), which was tried for three cases of severe traumatic brain injury (TBI) in rehabilitation. DESIGN:Case series. SETTING:University hospital setting. PARTICI...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-120289
更新日期：2013-01-01 00:00:00
abstract：PURPOSE:To determine how age at the time of left middle cerebral artery stroke affects language lateralization in a combined sample of subjects with perinatal, childhood, and adult stroke. METHODS:19 participants who had perinatal stroke (<1 month of age), 32 with later stroke, and 51 sex-/age-matched healthy controls...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-140402
更新日期：2014-01-01 00:00:00
abstract：:In the present study, we have developed an animal model for central nervous system (CNS) damage using the graded crush injury of the adult rat optic nerve. This injury model has the following characteristics: (a) the injuries are reproducible; (b) the injuries can be created with controlled severity; and (c) variation...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-1990-2104
更新日期：1990-01-01 00:00:00
abstract：PURPOSE:Repairing the spinal cord with peripheral nerve grafts (PNG) and adjuvant acidic fibroblast growth factor (FGF1) has previously resulted in partial functional recovery. To aid microsurgical placement of PNGs, a graft holder device was previously developed by our group. In hope for a translational development we...
journal_title：Restorative neurology and neuroscience
pub_type： 杂志文章
doi：10.3233/RNN-2011-0623
更新日期：2012-01-01 00:00:00
