Abstract:
:Oligodendrocyte precursor cells (OPCs) of the optic nerve are generated in the preoptic area, from where they migrate to colonize it entirely. Sonic hedgehog (Shh) induces the proliferation of these cells as well as influencing their migration, acting through its canonical receptor (Ptc-1). However, the multiligand receptor megalin (or LRP-2) is also involved in Shh-induced OPC proliferation and migration, and thus, we have evaluated the relevance of this interaction. During the stages at which Shh influences OPC development, we found megalin to be selectively expressed by optic nerve astrocytes, whereas Ptc-1 and Gli1 were found in OPCs. Indeed, this pattern of expression paralleled the rostral-caudal expression of the three Shh-related molecules during the time course of plp-dm20(+) -OPC colonization. The blockage of megalin partially abolished OPC chemoattraction and fully impaired Shh-induced proliferation. Using in vitro co-cultures of dissociated optic nerve cells, we demonstrated that Shh was internalized by astrocytes via megalin, and sufficient Shh was subsequently released to produce the biological effects on OPCs observed in the nerve. Together, these data indicate that at least part of the influence of Shh on OPCs is mediated by megalin during optic nerve development, and that astrocytes expressing megalin transiently capture Shh to present it to OPCs and/or to control the gradient of this molecule during development.
journal_name
Gliajournal_title
Gliaauthors
Ortega MC,Cases O,Merchán P,Kozyraki R,Clemente D,de Castro Fdoi
10.1002/glia.22316subject
Has Abstractpub_date
2012-05-01 00:00:00pages
851-66issue
6eissn
0894-1491issn
1098-1136journal_volume
60pub_type
杂志文章相关文献
GLIA文献大全abstract::Reorganization of the adult dentate gyrus following unilateral entorhinal cortex lesion (ECL) is a well-established model for studying mechanisms of trauma-induced neuronal plasticity. The lesion induces deafferentiation of the outer molecular layer, which is accompanied by a strong astroglial reaction. This glial res...
journal_title:Glia
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::This article reviews how the uptake of neurotransmitter by glial amino acid transporters limits the spatial spread of transmitter to preserve the independent operation of nearby synapses, temporally shapes postsynaptic currents, and regulates the effects of tonic transmitter release. We demonstrate the importance of a...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/glia.20027
更新日期:2004-08-15 00:00:00
abstract::Pigment epithelium-derived factor (PEDF) is a potent and broadly acting neurotrophic factor that protects neurons in various types of cultured neurons against glutamate excitotoxicity and induced-apoptosis. Some of the effects of PEDF reflect specific changes in gene expression, mediated via activation of the transcri...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20171
更新日期:2005-05-01 00:00:00
abstract::In the present study the distribution of astrocytes in the nerve fiber layer (NFL) has been studied in the sparsely vascularized retinae of the guinea pig and horse and in the richly vascularized retina of the Old World monkey (Cercopithecus aethiops) using immunocytochemical methods. In the guinea pig retina glial fi...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440010109
更新日期:1988-01-01 00:00:00
abstract::Oligodendrocyte precursor cells (OPCs) differentiate and mature into oligodendrocytes, which produce myelin in the central nervous system. Prior studies have shown that the mechanistic target of rapamycin (mTOR) is necessary for proper myelination of the mouse spinal cord and that bone morphogenetic protein (BMP) sign...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23776
更新日期:2020-06-01 00:00:00
abstract::Kainate (KA), quisqualate (QA), and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) stimulated gamma-aminobutyric acid [3H]gamma-aminobutyric acid (GABA) release from cultured cerebellar type 2 astrocytes and from their bipotential precursors. The evoked release was prevented by the antagonist 6-cyano-...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440040302
更新日期:1991-01-01 00:00:00
abstract::Knowledge of the precise timing of myelination is critical to the success of zebrafish-based in vivo screening strategies for potential remyelination therapies. This study provides a systematic review of the timing of myelination in the zebrafish spinal cord and a critique of techniques by which it may be accurately a...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20964
更新日期:2010-05-01 00:00:00
abstract::Astrocytes play a critical role in the development of the CNS and its response to injury and disease. A key indicator of astrocyte activation is the increased accumulation of intermediate filaments composed of glial fibrillary acidic protein (GFAP). Treatment of astrocytes in vitro with transforming growth factor-beta...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(sici)1098-1136(199802)22:2<202::aid-glia1
更新日期:1998-02-01 00:00:00
abstract::Integrins are transmembrane receptors that mediate cell-extracellular matrix and cell-cell interactions. The β1-integrin subunit is highly expressed by embryonic neural stem cells (NSCs) and is critical for NSC maintenance in the developing nervous system, but its role in the adult hippocampal niche remains unexplored...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22996
更新日期:2016-07-01 00:00:00
abstract::Pax-3 is a paired domain transcription factor that plays many roles during vertebrate development. In the Schwann cell lineage, Pax-3 is expressed at an early stage in Schwann cells precursors of the embryonic nerve, is maintained in the nonmyelinating cells of the adult nerve, and is upregulated in Schwann cells afte...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22346
更新日期:2012-09-01 00:00:00
abstract::Remyelination is in the center of new therapies for the treatment of multiple sclerosis to resolve and improve disease symptoms and protect axons from further damage. Although remyelination is considered beneficial in the long term, it is not known, whether this is also the case early in lesion formation. Additionally...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23167
更新日期:2017-08-01 00:00:00
abstract::Microglial cells have a double life as the immune cells of the brain in times of stress but have also specific physiological functions in homeostatic conditions. In pathological contexts, microglia undergo a phenotypic switch called "reaction" that promotes the initiation and the propagation of neuro-inflammation. Rea...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23295
更新日期:2018-05-01 00:00:00
abstract::The synthesis, transport, and insertion of jimpy proteolipid protein and DM20 were studied in normal (158N) and jimpy (158JP) immortalized oligodendrocyte lines. Four different expression vectors encoding fusion proteins composed of native PLP and DM20 or jimpy PLP or DM20 were linked to enhanced green fluorescent pro...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.10122
更新日期:2002-12-01 00:00:00
abstract::Prominin-1 (CD133) is a cholesterol-interacting pentaspan membrane glycoprotein specifically associated with plasma membrane protrusions. Prominin-1 is expressed by various stem and progenitor cells, notably neuroepithelial progenitors found in the developing embryonic brain. Here, we further investigated its expressi...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20812
更新日期:2009-06-01 00:00:00
abstract::Toll-like receptors 7 (TLR7) and 9 (TLR9) are important mediators of innate immune responses. Both receptors are located in endosomal compartments, recognize nucleic acids, and signal via Myeloid differentiation factor 88 (MyD88). In the current study, we analyzed TLR7 and TLR9 induced activation of astrocytes and mic...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20952
更新日期:2010-04-15 00:00:00
abstract::Zinc finger transcription factors of the Krüppel-class figure prominently in cell fate specification and differentiation in the nervous system. One of the Krüppel-type genes that was originally cloned from an oligodendrocyte library by virtue of its homology with the prototypic Krüppel motif is the rat rKr2 gene (Pott...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.1046
更新日期:2001-04-15 00:00:00
abstract::We have compared the effects of platelet-derived (PDGF) and basic fibroblast (bFGF) growth factors on the shape, migration, and differentiation of oligodendrocyte progenitor cells, the precursors of myelin-forming cells in the CNS. In the presence of bFGF, oligodendrocyte progenitors purified from rat neonatal brain c...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440070308
更新日期:1993-03-01 00:00:00
abstract::Minocycline, a derivative of the antibiotic tetracycline, displays neuroprotective properties in various models of neurodegenerative diseases and is now used in clinical trials, because of its relative safety and tolerability. Minocycline passes the blood-brain barrier and is presumed to inhibit microglial activation....
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20268
更新日期:2006-02-01 00:00:00
abstract::We first classified 12 malignant glioma cell lines into three different groups (types 1-3) with respect to major histocompatibility complex (MHC) class II expression and analyzed each group based on the different expression status of the class II transactivator (CIITA) gene. Glioma type 1 (2 of 12) showed constitutive...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.10343
更新日期:2004-03-01 00:00:00
abstract::Herpes simplex virus Type I (HSV-1) is a neurotropic virus that is capable of infecting not only neurons, but also microglia and astrocytes and can establish latent infection in the central nervous system (CNS). We investigated whether IFN lambda (IFN-λ), a newly identified member of IFN family, has the ability to inh...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.21076
更新日期:2011-01-01 00:00:00
abstract::Gliomas are a heterogenous group of malignant primary brain tumors that arise from glia cells or their progenitors and rely on accurate diagnosis for prognosis and treatment strategies. Although recent developments in the molecular biology of glioma have improved diagnosis, classical histological methods and biomarker...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23594
更新日期:2019-08-01 00:00:00
abstract::Manganese in excess is neurotoxic and causes CNS injury resembling that of Parkinson's disease. In brain, astrocytes predominantly take up and accumulate manganese and are thus vulnerable to its toxicity. Manganese was shown to induce cell swelling in cultured astrocytes, and oxidative/nitrosative stress (ONS) mediate...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.21023
更新日期:2010-09-01 00:00:00
abstract::We have attempted to reconstruct in vitro the events that may occur in vivo during reperfusion injury after ischemia in the central nervous system. The phenomenon is induced by previous exposure to low calcium solutions ("calcium paradox") before the reperfusion episode. Intracellular calcium alterations during reperf...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440050109
更新日期:1992-01-01 00:00:00
abstract::Serum is used widely for culturing neurons and glial cells, and is thought to provide essential, albeit undefined, factors such as hormones, growth factors, and trace elements that promote the growth of cells in vitro. Moreover, serum can have profound effects on cell proliferation, differentiation, and cell morpholog...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(sici)1098-1136(199803)22:3<237::aid-glia3
更新日期:1998-03-01 00:00:00
abstract::Poly(ADP-ribose) polymerase-1 (PARP-1) is a ubiquitous nuclear enzyme involved in genomic stability. Excessive oxidative DNA strand breaks lead to PARP-1-induced depletion of cellular NAD(+), glycolytic rate, ATP levels, and eventual cell death. Glutamate neurotransmission is tightly controlled by ATP-dependent astroc...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20936
更新日期:2010-03-01 00:00:00
abstract::We have developed a novel method of isolating and culturing murine colonic mucosal glial cells. Two morphologies are appreciated, a small flat bi or tri polar cell and a larger multipolar cell. The glial cultures have been freed of contaminating fibroblasts and epithelial cells and have been passaged by trypsinization...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440120204
更新日期:1994-10-01 00:00:00
abstract::Apoptosis of dentate granular cells in the hippocampal formation during bacterial meningitis may be mediated by glutamate toxicity. For this reason, we studied the relationship between glutamine synthetase activity and regional neuronal apoptosis in rabbits with experimental pneumococcal meningitis. The duration of me...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(sici)1098-1136(200003)30:1<11::aid-glia2>
更新日期:2000-03-01 00:00:00
abstract::Oxidative stress is the main cause of neuronal damage in traumatic brain injury, hypoxia/reperfusion injury, and neurodegenerative disorders. Although extracellular nucleosides, especially adenosine, are well known to protect against neuronal damage in such pathological conditions, the effects of these nucleosides or ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20118
更新日期:2005-01-15 00:00:00
abstract::Microglial erythrophagocytosis is crucial in injury response to hemorrhagic stroke. We hypothesized that regulation of microglial erythrophagocytosis via HO-1/CO depends on a pathway involving reactive oxygen species (ROS) and CD36 surface-expression. The microglial BV-2 cell line and primary microglia (PMG) were incu...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23864
更新日期:2020-11-01 00:00:00
abstract::Microglia are CNS resident immune cells and a rich source of neuroactive mediators, but their contribution to physiological brain processes such as synaptic plasticity, learning, and memory is not fully understood. In this study, we used mice with partial depletion of IκB kinase β, the main activating kinase in the in...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22756
更新日期:2015-04-01 00:00:00