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High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells.

Abstract：

:Murine embryonic stem cell (mESC)-derived cardiomyocytes represent a promising source of cells for use in the development of models for studying early cardiac development as well as cell-based therapies in postnatal pathologies. Here, we report a highly efficient cardiac differentiation system in which high density embryoid body (EB) cultures leads to a marked increase of cardiomyocytes production from multiple mESC lines without the addition of any cardiogenic growth factors. Our results show that high density EB cultures significantly increase the yield of functional cardiomyocytes, which express typical cardiac markers, exhibit normal rhythmic Ca(2+) transients, and respond to both β-adrenergic and electric stimulations. During the differentiation period, the inhibition of bone morphogenetic protein (BMP) signaling significantly attenuates the increase of cardiac differentiation as well as the increased expression of cardiac-specific genes, NK2 transcription factor related 5 (Nkx2.5) and myosin light chain 2v (Mlc2v) by high density EB cultures. Therefore, we believe that we offer a novel and efficient means of cardiomyocyte production for practical use of mESCs in cardiac regenerative medicine.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Lee MY,Cagavi Bozkulak E,Schliffke S,Amos PJ,Ren Y,Ge X,Ehrlich BE,Qyang Y

doi

10.1016/j.bbrc.2011.10.140

subject

Has Abstract

pub_date

2011-12-09 00:00:00

pages

51-7

issue

1-2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(11)01974-7

journal_volume

416

pub_type

杂志文章

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