Noninvasive and real-time monitoring of the therapeutic response of tumors in vivo with an optimized hTERT promoter.


BACKGROUND:Telomerase is commonly recognized as an effective anticancer target. The human telomerase reverse transcriptase (hTERT), the rate-limiting component of telomerase, is expressed in most malignant tumors, but it is not found in most normal somatic cells. Here, we report a real-time and noninvasive method to monitor tumor response to a lentivirus-based hTERT-conditional suicidal gene therapy. METHODS:In this study, we constructed a lentivirus system in which an optimized hTERT promoter was used to drive the expression of the cytosine deaminase (CD) gene, one of the suicide genes, and a green fluorescent protein (GFP) reporter gene (pLenti-CD/GFP). The lentivirus was used to infect telomerase-positive or telomerase-negative cell lines. In vitro and in vivo experiments were conducted to analyze the dynamic processes of exogenous gene expression noninvasively in cell culture and living animals in real time via optical imaging. RESULTS:The lentivirus was able to express the CD gene and GFP in telomerase-positive tumor cells and significantly decrease cell proliferation after the use of prodrug 5-flucytosine. However, it could not express GFP and CD in telomerase-negative cell lines, nor could it induce any suicidal effect in those cells. The in vivo study showed that telomerase-positive tumors can be visualized after intratumor injection of the lentivirus in tumor-bearing nude mice via an optical imaging system. Significant tumor growth suppression was observed in telomerase-positive tumors. CONCLUSIONS:Collectively, this technology provides a valuable, noninvasive method to evaluate the real-time therapeutic response of tumors in vivo.






Yu ST,Li C,Lü MH,Liang GP,Li N,Tang XD,Wu YY,Shi CM,Chen L,Li CZ,Cao YL,Fang DC,Yang SM




Has Abstract


2012-04-01 00:00:00














  • Detection of recurrent or persistent nasopharyngeal carcinomas after radiotherapy with technetium-99m methoxyisobutylisonitrile single photon emission computed tomography and computed tomography: comparison with 18-fluoro-2-deoxyglucose positron emission

    abstract:BACKGROUND:The diagnostic accuracy of technetium-99m methoxyisobutylisonitrile (Tc-MIBI) single photon emission computed tomography (SPECT) and computed tomography (CT) of the head and neck for differentiating recurrent or residual nasopharyngeal carcinomas (NPC) from benign lesions after radiotherapy was compared with...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Kao CH,Shiau YC,Shen YY,Yen RF

    更新日期:2002-04-01 00:00:00

  • Cell proliferative activity in adenomatous hyperplasia of the liver and small hepatocellular carcinoma. An immunohistochemical study demonstrating proliferating cell nuclear antigen.

    abstract:BACKGROUND:Recently, adenomatous hyperplasia (AH) of the liver, a sizable parenchymal nodule in the cirrhotic liver, has been considered a preneoplastic lesion in human hepatocarcinogenesis. METHODS:The authors evaluated cell proliferative activity by immunostaining for proliferating cell nuclear antigen (PCNA) in AH ...


    pub_type: 杂志文章


    authors: Terada T,Nakanuma Y

    更新日期:1992-08-01 00:00:00

  • Squamous cell carcinoma in situ of the endometrium and fallopian tube as superficial extension of invasive cervical carcinoma.

    abstract::Five cases of squamous cell carcinoma of the cervix associated with widespread squamous cell carcinoma in situ of the endometrial surface are reported. In one case, carcinoma in situ was also found in one fallopian tube in continuity with the cervicoendometrial lesion. A survey of the literature reveals only 20 cases ...


    pub_type: 杂志文章,评审


    authors: Kanbour AI,Stock RJ

    更新日期:1978-08-01 00:00:00

  • Induction of apoptosis and suppression of clonogenicity of ovarian carcinoma cells with estrogen mustard.

    abstract:BACKGROUND:This study was conducted to evaluate whether estramustine (estrogen mustard [EM]) is a promising alternative in the treatment of patients with epithelial ovarian carcinoma (OVCA). EM is a microtubule-associated protein (MAP) specific antimicrotubule agent with low toxicity. METHODS:The authors investigated ...


    pub_type: 杂志文章


    authors: Chen Z,Fadiel A,Jia JF,Sakamoto H,Carbone R,Naftolin F

    更新日期:1999-06-15 00:00:00

  • Anaerobic liver abscess and intrahepatic metastases: a case report and review of the literature.

    abstract::A patient is described in whom the first recurrence of a cloacogenic carcinoma of the rectum was an intrahepatic metastasis associated with an hepatic abscess caused by the anaerobic bacterium Peptococcus prevotii. Three previously reported cases of infection associated with hepatic tumor nodules have been found in wh...


    pub_type: 杂志文章,评审


    authors: Trump DL,Fahnestock R,Cloutier CT,Dickman MD

    更新日期:1978-02-01 00:00:00

  • Carcinoma of the esophagus with adenoid cystic differentiation.

    abstract::Adenoid cystic carcinoma of the esophagus is a relatively rare lesion which characteristically exhibits a clinically aggressive behavior. In spite of this aggressive nature, it is most often referred to as a counterpart of the more common adenoid cystic carcinoma of salivary gland origin, a comparatively indolent tumo...


    pub_type: 杂志文章,评审


    authors: Epstein JI,Sears DL,Tucker RS,Eagan JW Jr

    更新日期:1984-03-01 00:00:00

  • The role of lymph node dissection in the treatment of gallbladder carcinoma.

    abstract:BACKGROUND:Lymph node involvement is an important prognostic factor in gallbladder carcinoma (GBC). The lymph node involvement pattern, extent, and indications for systematic lymph node dissection for patients with advanced GBC were investigated. METHODS:Forty-one patients with GBC who underwent radical resection with...


    pub_type: 杂志文章


    authors: Shimada H,Endo I,Togo S,Nakano A,Izumi T,Nakagawara G

    更新日期:1997-03-01 00:00:00

  • Breast conservation therapy in the United States following the 1990 National Institutes of Health Consensus Development Conference on the treatment of patients with early stage invasive breast carcinoma.

    abstract:BACKGROUND:A National Institutes of Health (NIH) Consensus Development Conference on the treatment of patients with early stage invasive breast carcinoma, held in June 1990, recommended breast conservation therapy for the majority of women with Stage I or II breast carcinoma. The authors evaluated the national use of b...


    pub_type: 杂志文章


    authors: Lazovich D,Solomon CC,Thomas DB,Moe RE,White E

    更新日期:1999-08-15 00:00:00

  • Early changes in regenerating hamster pancreas following a single dose of N-nitrosobis (2-oxopropyl)amine (NBOP) administered at the peak of DNA synthesis.

    abstract::A single dose of N-nitrosobis(2-oxopropyl)amine, a carcinogen for hamster pancreas, was administered to hamsters with regenerating pancreas 60 hours after initiation of regeneration when the maximum number of acinar cells are in S phase of the cell cycle. This led to nucleolar segregation and mitotic abnormalities fro...


    pub_type: 杂志文章


    authors: Scarpelli DG,Rao MS

    更新日期:1981-03-15 00:00:00

  • Real-time tumor-tracking radiation therapy for lung carcinoma by the aid of insertion of a gold marker using bronchofiberscopy.

    abstract:BACKGROUND:The authors developed fluoroscopic real-time tumor-tracking radiation therapy (RTRT) by insertion of a gold marker using bronchofiberscopy to reduce uncertainties in organ motion and set-up error in external radiotherapy for moving tumors. The purpose of the current study was to evaluate RTRT's feasibility i...


    pub_type: 临床试验,杂志文章


    authors: Harada T,Shirato H,Ogura S,Oizumi S,Yamazaki K,Shimizu S,Onimaru R,Miyasaka K,Nishimura M,Dosaka-Akita H

    更新日期:2002-10-15 00:00:00

  • Clinical experience with leucovorin and 5-fluorouracil.

    abstract::Two trials of leucovorin (LV) and 5-fluorouracil (5-FU) in patients with metastatic colorectal cancer were done, both using a 3-day loading dose and then weekly doses to minimize toxicity. The first trial used LV administered by intravenous infusion with a constant dose of 5-FU 400 mg/m2, and the second trail used ora...


    pub_type: 杂志文章


    authors: Laufman LR,Brenckman WD Jr,Stydnicki KA,Morgan ED,Collier M,Knick VB,Duch DS,Mullin R,Ferone R

    更新日期:1989-03-15 00:00:00

  • Clear cell sarcoma of tendons and aponeuroses in pediatric patients: a report from the Italian and German Soft Tissue Sarcoma Cooperative Group.

    abstract:BACKGROUND:Clear cell sarcoma (CCS) of tendons and aponeuroses is extremely rare in childhood and little information is available on its clinical management. Originally believed to be a type of melanoma of soft tissue origin, CCS is now considered a distinct clinicopathologic entity that behaves like a high-grade soft ...


    pub_type: 杂志文章


    authors: Ferrari A,Casanova M,Bisogno G,Mattke A,Meazza C,Gandola L,Sotti G,Cecchetto G,Harms D,Koscielniak E,Treuner J,Carli M

    更新日期:2002-06-15 00:00:00

  • Colorectal carcinoma cell production of transforming growth factor beta decreases expression of endothelial cell vascular endothelial growth factor receptor 2.

    abstract:BACKGROUND:Vascular endothelial growth factor (VEGF) signaling is a target for antiangiogenic cancer therapy. The authors have previously observed that up to 40% of vessels in colorectal carcinoma (CRC) tumors are negative for VEGF receptor 2 (VEGFR2) expression. Differential activity of transforming growth factor beta...


    pub_type: 杂志文章


    authors: Kuczynski EA,Viloria-Petit AM,Coomber BL

    更新日期:2011-12-15 00:00:00

  • Local relapse and contralateral tumor rates in patients with breast cancer treated with conservative surgery and radiotherapy (Institut Gustave Roussy 1970-1982). IGR Breast Cancer Group.

    abstract:BACKGROUND:Breast conservation is now established treatment for patients with small breast cancers. The authors reviewed a large series of patients with long term follow-up who underwent conservative treatment. Clinical and pathologic factors were analyzed to identify patients at an increased risk of relapse in the bre...


    pub_type: 杂志文章


    authors: Dewar JA,Arriagada R,Benhamou S,Benhamou E,Bretel JJ,Pellae-Cosset B,Marin JL,Petit JY,Contesso G,Sarrazin D

    更新日期:1995-12-01 00:00:00

  • Phase 1 study of an oxaliplatin and etoposide regimen in pediatric patients with recurrent solid tumors.

    abstract:BACKGROUND:The combination of a platinating agent and etoposide has induced responses in various pediatric tumors. The study estimated the maximum tolerated dose (MTD) of an oxaliplatin and etoposide regimen in children with recurrent solid tumors. METHODS:Oxaliplatin was administered on Day 1 and etoposide on Days 1 ...


    pub_type: 杂志文章


    authors: McGregor LM,Spunt SL,Santana VM,Stewart CF,Ward DA,Watkins A,Laningham FH,Ivy P,Furman WL,Fouladi M

    更新日期:2009-02-01 00:00:00

  • Preoperative chemotherapy for osteogenic sarcoma: selection of postoperative adjuvant chemotherapy based on the response of the primary tumor to preoperative chemotherapy.

    abstract::Since June 1978, 57 patients with primary osteogenic sarcoma of an extremity were treated with high-dose methotrexate (HDMTX) and citrovorum factor rescue (CFR), Adriamycin, and the combination of bleomycin, cyclophosphamide and dactinomycin (BCD) given for 4-16 weeks prior to definitive surgery. Histologic examinatio...


    pub_type: 杂志文章


    authors: Rosen G,Caparros B,Huvos AG,Kosloff C,Nirenberg A,Cacavio A,Marcove RC,Lane JM,Mehta B,Urban C

    更新日期:1982-03-15 00:00:00

  • Unexpectedly frequent hepatitis B reactivation by chemoradiation in postgastrectomy patients.

    abstract:BACKGROUND:Postgastrectomy patients undergoing chemoradiation risk chemoradiation-induced liver disease (CRILD). The objectives of this study were to investigate dosimetric implications and assess biologic susceptibility to CRILD in these patients. METHODS:Sixty-two patients with Stage IB-IV gastric/gastroesophageal a...


    pub_type: 杂志文章


    authors: Cheng JC,Liu MC,Tsai SY,Fang WT,Jer-Min Jian J,Sung JL

    更新日期:2004-11-01 00:00:00

  • A comparison of screening mammography results from programs for women of different socioeconomic status.

    abstract:BACKGROUND:The ability of screening mammography programs serving women of different socioeconomic status (SES) to diagnose early stage breast carcinoma in a comparably effective fashion has been questioned. METHODS:Results of screening 50,653 women of lower SES were compared with those of screening 45,923 more socioec...


    pub_type: 杂志文章


    authors: Dershaw DD,Loring L,True SJ,Momrow DC,Liberman L,Morris EA

    更新日期:1998-05-01 00:00:00

  • The emerging role of mediator complex subunit 12 in tumorigenesis and response to chemotherapeutics.

    abstract::Transcriptional dysregulation induced by disease-defining genetic alterations of proteins in transcriptional machinery is a key feature of cancers. Mediator complex subunit 12 (MED12) is the central architectural subunit in the kinase module of Mediator, a large transcriptional regulatory complex that controls essenti...


    pub_type: 杂志文章,评审


    authors: Zhang S,O'Regan R,Xu W

    更新日期:2020-03-01 00:00:00

  • Adjuvant radiotherapy versus combined sequential chemotherapy followed by radiotherapy in the treatment of resected nonsmall cell lung carcinoma. A randomized trial of 267 patients. GETCB (Groupe d'Etude et de Traitement des Cancers Bronchiques).

    abstract:BACKGROUND:The effect of adjuvant chemotherapy after resection of nonsmall cell lung cancer (NSCLC) remains an unresolved question. METHODS:From October, 1982, to November, 1986, 267 patients with resected NSCLC were included in a randomized trial. The adjuvant allocated treatments were either postoperative radiothera...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Dautzenberg B,Chastang C,Arriagada R,Le Chevalier T,Belpomme D,Hurdebourcq M,Lebeau B,Fabre C,Charvolin P,Guérin RA

    更新日期:1995-09-01 00:00:00

  • Typhlitis in childhood cancer.

    abstract:BACKGROUND:Typhlitis is increasingly recognized in children undergoing chemotherapy but is poorly characterized. The authors investigated the demographic, clinical, and imaging (ultrasonography and computed tomography [CT] scans) variables related to the diagnosis, risk, and outcome of typhlitis. METHODS:The authors r...


    pub_type: 杂志文章


    authors: McCarville MB,Adelman CS,Li C,Xiong X,Furman WL,Razzouk BI,Pui CH,Sandlund JT

    更新日期:2005-07-15 00:00:00

  • Adenosarcoma of uterus and ovary: a clinicopathologic study of two cases.

    abstract::We report the clinical and pathologic features of two cases of adenosarcoma. Our first case occurred in the ovary of a 51-year-old woman with extensive pelvic involvement. She was treated vigorously with radiation and chemotherapy and at the present time almost 9 years later the patient is free of disease. This repres...


    pub_type: 杂志文章


    authors: Valdez VA,Planas AT,Lopez VF,Goldberg M,Herrera NE

    更新日期:1979-04-01 00:00:00

  • Therapy of meningeal melanocytomas.

    abstract:BACKGROUND:Meningeal melanocytomas are rare, benign central nervous system lesions with a high probability of recurrence. To the authors' knowledge, approximately 100 cases have been reported since 1972, when the entity first was described. In the current study, four therapies were compared with regard to local control...


    pub_type: 杂志文章


    authors: Rades D,Schild SE,Tatagiba M,Molina HA,Alberti W

    更新日期:2004-06-01 00:00:00

  • Changes in treatment patterns for patients with locally advanced rectal cancer in the United States over the past decade: An analysis from the National Cancer Data Base.

    abstract:BACKGROUND:In the United States, neoadjuvant chemoradiotherapy (NACRT) is widely accepted as the standard of care in the treatment of patients with locally advanced rectal cancer. In the current study, the authors attempted to examine patterns of treatment in the United States over the past decade. METHODS:Using the N...


    pub_type: 杂志文章


    authors: Sineshaw HM,Jemal A,Thomas CR Jr,Mitin T

    更新日期:2016-07-01 00:00:00

  • Increase in primary surgical treatment of T1 and T2 oropharyngeal squamous cell carcinoma and rates of adverse pathologic features: National Cancer Data Base.

    abstract:BACKGROUND:There has been increasing interest in the primary surgical treatment of patients with early T classification (T1-T2) oropharyngeal squamous cell carcinoma (OPSCC), with the stated goal of de-escalating or avoiding adjuvant treatment. Herein, the authors sought to determine the degree to which this interest h...


    pub_type: 杂志文章


    authors: Cracchiolo JR,Baxi SS,Morris LG,Ganly I,Patel SG,Cohen MA,Roman BR

    更新日期:2016-05-15 00:00:00

  • Influence of provider factors and race on uptake of breast cancer gene expression profiling.

    abstract:BACKGROUND:Gene expression profiling (GEP) has been rapidly adopted for early breast cancer and can aid in chemotherapy decision making. Study results regarding racial disparities in testing are conflicting, and may reflect different care settings. To the authors' knowledge, data regarding the influence of provider fac...


    pub_type: 杂志文章


    authors: Reeder-Hayes KE,Wheeler SB,Baggett CD,Zhou X,Meng K,Roberts MC,Carey LA,Meyer AM

    更新日期:2018-04-15 00:00:00

  • Comparative analysis of the sensitivity of endometrial cancer cells to epidermal growth factor and steroid hormones.

    abstract:BACKGROUND:Epidermal growth factor (EGF) and EGF-regulated processes play an important role in steroid signal transduction. Comparative analysis of EGF and steroid receptor expression and the sensitivity of early stages of proliferation induction, such as activation of phospholipid turnover to EGF and steroids, may pro...


    pub_type: 杂志文章


    authors: Gershtein ES,Shatskaya VA,Kostyleva OI,Ermilova VD,Kushlinsky NE,Krasil'nikov MA

    更新日期:1995-12-15 00:00:00

  • Phantom breast sensations following mastectomy.

    abstract::Eighty-nine patients were studied for the incidence and nature of phantom breast sensations (PBS) after a modified mastectomy. Twenty-nine (33%) reported experiencing pain or itching in the breast or the more vague sensation that the breast was still present. Phantom breast sensations appeared generally within the fir...


    pub_type: 杂志文章


    authors: Staps T,Hoogenhout J,Wobbes T

    更新日期:1985-12-15 00:00:00

  • Attrition rates, reasons, and predictive factors in supportive care and palliative oncology clinical trials.

    abstract:BACKGROUND:Attrition is common among supportive care/palliative oncology clinical trials. However, to the authors' knowledge, few studies to date have documented the reasons and predictors for dropout. In the current study, the authors' objective was to determine the rate, reasons, and factors associated with attrition...


    pub_type: 杂志文章


    authors: Hui D,Glitza I,Chisholm G,Yennu S,Bruera E

    更新日期:2013-03-01 00:00:00

  • Treatment of advanced Hodgkin's disease with B-CAVE following MOPP failure.

    abstract::Between March 1973, and December 1976, 22 patients who developed disease progression during or after MOPP therapy were treated with a new combination, B-CAVe (Bleomycin 5 mg/m2 iv days 1, 28, 35; CCNU 100 mg/m2 po day 1; adriamycin 60 mg/m2 iv day 1; and vinblastine 5 mg/m2 iv day 1). Objective responses were achieved...


    pub_type: 杂志文章


    authors: Porzig KJ,Portlock CS,Robertson A,Rosenberg SA

    更新日期:1978-05-01 00:00:00