Abstract:
BACKGROUND:Telomerase is commonly recognized as an effective anticancer target. The human telomerase reverse transcriptase (hTERT), the rate-limiting component of telomerase, is expressed in most malignant tumors, but it is not found in most normal somatic cells. Here, we report a real-time and noninvasive method to monitor tumor response to a lentivirus-based hTERT-conditional suicidal gene therapy. METHODS:In this study, we constructed a lentivirus system in which an optimized hTERT promoter was used to drive the expression of the cytosine deaminase (CD) gene, one of the suicide genes, and a green fluorescent protein (GFP) reporter gene (pLenti-CD/GFP). The lentivirus was used to infect telomerase-positive or telomerase-negative cell lines. In vitro and in vivo experiments were conducted to analyze the dynamic processes of exogenous gene expression noninvasively in cell culture and living animals in real time via optical imaging. RESULTS:The lentivirus was able to express the CD gene and GFP in telomerase-positive tumor cells and significantly decrease cell proliferation after the use of prodrug 5-flucytosine. However, it could not express GFP and CD in telomerase-negative cell lines, nor could it induce any suicidal effect in those cells. The in vivo study showed that telomerase-positive tumors can be visualized after intratumor injection of the lentivirus in tumor-bearing nude mice via an optical imaging system. Significant tumor growth suppression was observed in telomerase-positive tumors. CONCLUSIONS:Collectively, this technology provides a valuable, noninvasive method to evaluate the real-time therapeutic response of tumors in vivo.
journal_name
Cancerjournal_title
Cancerauthors
Yu ST,Li C,Lü MH,Liang GP,Li N,Tang XD,Wu YY,Shi CM,Chen L,Li CZ,Cao YL,Fang DC,Yang SMdoi
10.1002/cncr.26476subject
Has Abstractpub_date
2012-04-01 00:00:00pages
1884-93issue
7eissn
0008-543Xissn
1097-0142journal_volume
118pub_type
杂志文章相关文献
CANCER文献大全abstract:BACKGROUND:Low frequency epithelial cells in bone marrow from colorectal carcinoma patients are associated with an increased risk of recurrence and decreased survival. Current immunohistochemical approaches to detect epithelial cells in bone marrow aspirates rely on antibodies against cytokeratin 18 (CK18). The predict...
journal_title:Cancer
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND:Quality of life (QOL) issues in patients with head and neck carcinoma are of importance beyond the incidence of these tumors because of the impact of the disease and its treatment on external appearance and function of the upper aerodigestive tract. Nasopharyngeal carcinoma (NPC) patients comprise a unique s...
journal_title:Cancer
pub_type: 杂志文章
doi:
更新日期:2002-02-15 00:00:00
abstract:BACKGROUND:Vascular endothelial growth factor receptor-1 (VEGFR-1) is one of three receptor tyrosine kinases for VEGF, a key regulator of angiogenesis in cancer. Although VEGFRs initially were believed to be expressed exclusively on endothelial cells (ECs), recent studies have demonstrated the presence of VEGFR-1 on no...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.21145
更新日期:2005-07-15 00:00:00
abstract::Flow cytometry (FC) analysis including DNA index (ploidy status) and cell kinetics (%S and %S + G2/M) was done on frozen tissue of the primary lesions of 101 women with node negative (N-) breast cancer who were studied prospectively. Currently, 19% (19/101) of the patients have recurred. No significant relations have ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19891101)64:9<1894::aid-cncr2820
更新日期:1989-11-01 00:00:00
abstract::From April 1985 through March 1988, 24 lung cancer patients with carcinomatous pleuritis were treated with a new combined treatment modality consisting of pulmonary resection and postoperative intrathoracic chemo-thermotherapy (PICT). They consisted of 15 male patients and nine female patients. A majority of the patie...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19891001)64:7<1422::aid-cncr2820
更新日期:1989-10-01 00:00:00
abstract::A specimen from the small intestine with multiple (three) classic carcinoid tumors and one appendiceal carcinoid tumor displaying argentaffinity, argyrophilia (Grimelius stain), and serotonin and neuron specific enolase immunoreactivity was examined by light microscopy with regard to the tumor cell histopathogenesis. ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19870715)60:2<201::aid-cncr28206
更新日期:1987-07-15 00:00:00
abstract::Cancer of the penis is infrequent in the United States because of good genital hygiene and circumcision of male babies at birth. In other countries, it represents 11-12% of all cancer in males. Tumors of the penis consist mainly of squamous cell carcinoma. Mesenchymal tumors are rare. The treatment is based on the sur...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.1980.45.s7.1973
更新日期:1980-04-01 00:00:00
abstract::The authors describe the results of a hospital-based incident case-control study of lung cancer conducted in a high-risk region of southern Louisiana from January 1979 through April 1982. Dietary intake of carotene, retinol, and vitamin C was estimated from food frequency questionnaires administered to 1253 cases and ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19881115)62:10<2267::aid-cncr282
更新日期:1988-11-15 00:00:00
abstract:BACKGROUND:Interleukin-18 (IL-18) is a novel immunoregulatory cytokine that was known previously as interferon-gamma-inducing factor. IL-18 levels can be used as a serum indicator for monitoring the clinical course of patients with hematologic malignancies and gastric carcinoma. Nitric oxide (NO) is a pleiotropic molec...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.10705
更新日期:2002-08-01 00:00:00
abstract::The descriptive epidemiologic features of bladder cancer and lung cancer in Los Angeles County were studied for the period 1972-1976, using data from the Los Angeles County/University of Southern California Cancer Surveillance Program. The incidence pattern for bladder cancer was found to differ markedly from the patt...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19830215)51:4<675::aid-cncr28205
更新日期:1983-02-15 00:00:00
abstract:BACKGROUND:The mortality rate associated with fever accompanied by pulmonary infiltrates after chemotherapy for hematologic malignancies remains higher than the corresponding rate associated with febrile neutropenia without pulmonary infiltrates. Nonetheless, few studies have focused on the factors that predict outcome...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.20406
更新日期:2004-08-01 00:00:00
abstract::In recent years a major focus in the delivery of cancer prevention services has been on the actions of physicians in ambulatory care practice. Whereas delivery of these services has increased markedly, it is likely that physician actions overall are not producing expected measurable benefits to whole populations. The ...
journal_title:Cancer
pub_type: 杂志文章,评审
doi:10.1002/1097-0142(19940815)74:4+<1418::aid-cncr282
更新日期:1994-08-15 00:00:00
abstract:BACKGROUND:Previous data from the National Cancer Data Base have examined time trends in stage of disease, treatment patterns, and survival for selected cancers. The most current (1993) data for breast carcinoma are described here. METHODS:Five Calls for Data have yielded a total of 508,724 breast cancer cases diagnos...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0142(19961015)78:8<1838::aid-cn
更新日期:1996-10-15 00:00:00
abstract::Both image and flow DNA cytometry were performed in isolated nuclei from paraffin-embedded tumor tissue of patients with small cell carcinoma of the lung (SCCL). In 14 patients tissue was obtained by surgery from the primary tumor. From 14 patients tissue was taken by autopsy. From two patients tissue obtained by both...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19890915)64:6<1304::aid-cncr2820
更新日期:1989-09-15 00:00:00
abstract:BACKGROUND:Controlled-release (CR) morphine tablets have become routine therapy in the management of cancer pain. Compared with immediate-release (IR) morphine, this formulation provides the benefit of dosing every 12 hours. METHODS:This study reviewed the 10 published, well controlled, repeated-dose, comparative stud...
journal_title:Cancer
pub_type: 杂志文章,评审
doi:10.1002/(sici)1097-0142(19980615)82:12<2299::aid-c
更新日期:1998-06-15 00:00:00
abstract:BACKGROUND:The possible association between ovarian carcinoma and ovulation-inducing drugs has led to renewed interest in the potential carcinogenic risks of these drugs. In vitro fertilization (IVF) has been linked to multiple pregnancies and possibly congenital malformations. However, to the authors' knowledge the as...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(20000615)88:12<2845::aid-cncr26>
更新日期:2000-06-15 00:00:00
abstract::A case of basosquamous or so-called transitional cloacogenic carcinoma of the sigmoid colon, which arose above the pelvic brim at the peritoneal reflection, is reported. We were not able to find a report of this histologic type of tumor arising this far from the pectinate line of the anus, which is the most common pri...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(197709)40:3<1234::aid-cncr282040
更新日期:1977-09-01 00:00:00
abstract:BACKGROUND:The purpose of the current study was to describe a population of young patients with breast carcinoma, their characteristics at the time of diagnosis, and the association of these characteristics with disease recurrence and survival. METHODS:Four hundred fifty-two women age < or = 35 years with breast carci...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.21070
更新日期:2005-06-15 00:00:00
abstract::One hundred twenty two children with non-Hodgkin's lymphoma were studied from January 1966 to December 1975. The first group (1966-1972) did not receive an uniform treatment. The second group (1973-1975) entered in a G.A.T.L.A. protocol consisting of: vincristine-prednisone plus surgery and/or radiotherapy as inductio...
journal_title:Cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/1097-0142(197806)41:6<2372::aid-cncr282041
更新日期:1978-06-01 00:00:00
abstract:BACKGROUND:As androgen deprivation therapy (ADT) becomes a standard of treatment for men with recurrent or metastatic prostate cancer, evaluation of adverse effects associated with this treatment is needed. In this study, the authors evaluated the effect of ADT administered as monotherapy and in combination with local ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.26064
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Smoking and alcoholic beverage drinking habits as well as a family history of cancer are well known risk factors for the multifocal occurrence of squamous cell carcinoma of the esophagus and the head and neck region. However, the role of these risk factors in multiple gastric carcinoma remains to be clarifie...
journal_title:Cancer
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::Compliance in oncology clinic attendance was prospectively evaluated over a 12-month period at Harlem Hospital Center, an inner city municipal facility serving a predominantly black population. One hundred patients were followed (97 blacks, 2 Hispanics, 1 white) with an average age of 60 years. There were 41 men and 5...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19860801)58:3<793::aid-cncr28205
更新日期:1986-08-01 00:00:00
abstract::Blood vessel invasion and axillary lymph node involvement were examined in 175 breast cancer patients. The incidence of blood vessel invasion was 35%. The presence of blood vessel invasion was highly associated with early disease recurrence. The association of poor prognosis with blood vessel invasion was independent ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19820901)50:5<962::aid-cncr28205
更新日期:1982-09-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to determine risk factors that affect locoregional control of nasopharyngeal carcinoma (NPC) after radiotherapy. Computed tomography (CT) is utilized for radiotherapy planning and for identifying high risk anatomic areas. METHODS:Between April 1990 and December 1993, 40 consecu...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0142(19980115)82:2<261::aid-cnc
更新日期:1998-01-15 00:00:00
abstract::Management of testis cancer in children differs from that in adults due to the histological types and varying tolerances to chemotherapy agents. Embryonal adrenocarcinoma (endodermal sinus tumor, Teilum's tumor) is a tumor of infancy and young children and less aggressive than other testis cancers. Most cases require ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.1980.45.s7.1803
更新日期:1980-04-01 00:00:00
abstract:BACKGROUND:Previous Commission on Cancer studies have examined time trends in stage of disease, treatment patterns, and survival for special populations. Reported herein are the most current National Cancer Data Base data for Hispanics. METHODS:Two Calls for Data have yielded a total of 23,650 cancer reports for Hispa...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19941015)74:8<2386::aid-cncr2820
更新日期:1994-10-15 00:00:00
abstract:BACKGROUND:The objective of this study was to determine whether a very low presenting prostate-specific antigen (PSA) level was associated with greater prostate cancer-specific mortality (PCSM) among men with a Gleason score (GS) of 8 to 10. METHODS:The Surveillance, Epidemiology, and End Results program was used to i...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.29691
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Nonsteroidal anti-inflammatory drugs (NSAIDs) may prevent the development of cancer by inhibiting cyclooxygenase (COX) enzymes, which are involved in carcinogenesis. Therefore, the authors of this report examined the association between NSAID use and the risk of squamous cell carcinoma (SCC), basal cell carc...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.27406
更新日期:2012-10-01 00:00:00
abstract::International comparisons have provided striking correlations between fat consumption and risk of breast cancer, but these comparisons do not often consider variations in life style. Case-control studies carried out in several countries showed no real association between fat intake and breast cancer. There is some evi...
journal_title:Cancer
pub_type: 杂志文章,评审
doi:10.1002/1097-0142(19900915)66:14+<1321::aid-cncr28
更新日期:1990-09-15 00:00:00
abstract::Twelve Schwann cell tumors (two neurilemomas, six neurofibromas, and four malignant schwannomas), arising in the nasal cavity, paranasal sinuses or nasopharynx, are described. Schwann cell neoplasms only rarely develop in this area. Clinically, these tumors lead to nonspecific symptoms including nasal obstruction epis...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19821115)50:10<2193::aid-cncr282
更新日期:1982-11-15 00:00:00