Knockdown of synaptic scaffolding protein Homer 1b/c attenuates secondary hyperalgesia induced by complete Freund's adjuvant in rats.


BACKGROUND:Previous studies have demonstrated that Homer 1b/c, a postsynaptic molecular scaffolding protein that binds and clusters metabotropic glutamate receptors at neuronal synapses, has an important role in the metabotropic glutamate receptor signaling process. In the current study, we investigated the possible involvement of Homer 1b/c in secondary hyperalgesia induced by complete Freund's adjuvant (CFA). METHODS:Chronic inflammation was induced by injecting CFA into the left hind ankle of Wistar rats. Homer 1b/c antisense or missense oligonucleotides were intrathecally administrated (antisense, 10 μg/10 μL, 5 μg/10 μL, or 2.5 μg/10 μL, once a day; missense, 10 μg/10 μL) from 5 to 8 days after the onset of inflammation. The withdrawal threshold and withdrawal latency to mechanical or thermal stimuli were determined before and after the intrathecal administration. The expression and distribution of Homer 1b/c were examined in the spinal cord using immunological techniques. RESULTS:Mechanical allodynia and thermal hyperalgesia were induced within 24 hours and maintained for >2 weeks after the CFA injection. The expression of Homer 1b/c reached the highest level 7 days after inflammation and returned to baseline at day 28. Intrathecal administration of Homer 1b/c antisense oligonucleotides markedly reduced the expression of Homer 1b/c protein in the spinal cord. Additionally, administration of Homer 1b/c antisense oligonucleotides attenuated secondary mechanical hypersensitization on days 2 to 5 and reduced thermal hypersensitization on days 3 to 4. There were no effects of missense oligonucleotides on hypersensitization and the expression of Homer 1b/c. In the naïve rats, Homer 1b/c antisense oligonucleotides did not affect the mechanical and thermal responses or locomotor activity. CONCLUSIONS:These novel results demonstrate that Homer 1b/c in the spinal cord contributes to the maintenance of secondary hyperalgesia induced by CFA and suggest that Homer 1b/c may be a novel target for pain therapy.


Anesth Analg


Anesthesia and analgesia


Yao YX,Jiang Z,Zhao ZQ




Has Abstract


2011-12-01 00:00:00














  • Sequential use of midazolam and propofol for long-term sedation in postoperative mechanically ventilated patients.

    abstract:UNLABELLED:Acute withdrawal syndromes, including agitation and a long weaning time, are common adverse effects after long-term sedation with midazolam. We performed this study to determine whether the sequential use of midazolam and propofol could reduce adverse effects as compared with midazolam alone. We studied 26 p...

    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Saito M,Terao Y,Fukusaki M,Makita T,Shibata O,Sumikawa K

    更新日期:2003-03-01 00:00:00

  • Effects of diazepam and midazolam on baroreflex control of heart rate and on sympathetic activity in humans.

    abstract::The effects of induction of anesthesia with diazepam and midazolam on baroreflex control of heart rate and on plasma levels of catecholamines were investigated in this study. Group 1 subjects (n = 10) received diazepam, 0.4 mg/kg. Group 2 subjects (n = 10) received midazolam, 0.3 mg/kg. Baroreflex function was assesse...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Marty J,Gauzit R,Lefevre P,Couderc E,Farinotti R,Henzel C,Desmonts JM

    更新日期:1986-02-01 00:00:00

  • Neostigmine Versus Sugammadex for Reversal of Neuromuscular Blockade and Effects on Reintubation for Respiratory Failure or Newly Initiated Noninvasive Ventilation: An Interrupted Time Series Design.

    abstract:BACKGROUND:Pulmonary complications related to residual neuromuscular blockade lead to morbidity and mortality. Using an interrupted time series design, we tested whether proportions of reintubation for respiratory failure or new noninvasive ventilation were changed after a system-wide transition of the standard reversa...

    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章


    authors: Krause M,McWilliams SK,Bullard KJ,Mayes LM,Jameson LC,Mikulich-Gilbertson SK,Fernandez-Bustamante A,Bartels K

    更新日期:2020-07-01 00:00:00

  • A method for testing for epinephrine-induced arrhythmias in rats.

    abstract::Evaluation of a new inhaled anesthetic requires determination of the anesthetic's potential to predispose to ventricular (and other) arrhythmias in an animal model. This need poses logistic and economic burdens if the effects of large numbers of new anesthetics are to be surveyed. We have devised a technique for deter...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Laster MJ,Johnson BH,Eger EI 2nd,Taheri S

    更新日期:1990-06-01 00:00:00

  • The effects of sevoflurane and hyperventilation on electrocorticogram spike activity in patients with refractory epilepsy.

    abstract:UNLABELLED:We investigated the effects of sevoflurane and hyperventilation on intraoperative electrocorticogram (ECoG) spike activity in 13 patients with intractable epilepsy. Grid electrodes were placed on the brain surface and ECoG was recorded under the following conditions: 1) 0.5 minimal alveolar anesthetic concen...

    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章


    authors: Kurita N,Kawaguchi M,Hoshida T,Nakase H,Sakaki T,Furuya H

    更新日期:2005-08-01 00:00:00

  • The pain visual analog scale: is it linear or nonlinear?

    abstract:UNLABELLED:The visual analog scale (VAS) is a tool widely used to measure pain, yet controversy surrounds whether the VAS score is ratio or ordinal data. We studied 52 postoperative patients and measured their pain intensity using the VAS. We then asked them to consider different amounts of pain (conceptually twice as ...

    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章


    authors: Myles PS,Troedel S,Boquest M,Reeves M

    更新日期:1999-12-01 00:00:00

  • Patient preferences for early discharge after laparoscopic cholecystectomy.

    abstract:UNLABELLED:Patients may have concerns about their ability to manage postoperative symptoms at home after ambulatory surgery. We assessed patients' attitudes toward postoperative care at home or in the hospital after laparoscopic cholecystectomy. Thirty-eight patients undergoing elective laparoscopic cholecystectomy wer...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Lehmann HP,Fleisher LA,Lam J,Frink BA,Bass EB

    更新日期:1999-06-01 00:00:00

  • Comparison of pH-adjusted bupivacaine 0.75% and a mixture of bupivacaine 0.75% and lidocaine 2%, both with hyaluronidase, in day-case cataract surgery under regional anesthesia.

    abstract::Alkalinized bupivacaine 0.75% (pH 6.8) and a mixture (1:1) of bupivacaine 0.75% and lidocaine 2%, both with hyaluronidase, were compared in regional ophthalmic anesthesia for day-case cataract surgery. Eighty-two patients were randomized into two groups (n = 39 and 43) to receive one of the two solutions in a double-b...

    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Sarvela PJ,Paloheimo MP,Nikki PH

    更新日期:1994-07-01 00:00:00

  • Nitric oxide synthase inhibitor blocks spinal sensitization induced by formalin injection into the rat paw.

    abstract::This study evaluated the role of nitric oxide (NO) in spinal cord nociceptive transmission during peripheral inflammation evoked by formalin injection into the rat paw, using N omega-nitro-L-arginine (N-Arg), an NO synthase inhibitor. Male rats were prepared with intrathecal (IT) catheters. To quantify the formalin re...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Yamamoto T,Shimoyama N,Mizuguchi T

    更新日期:1993-11-01 00:00:00

  • A comparison of peripheral skin blood flow and temperature during endoscopic thoracic sympathotomy.

    abstract::The assessment of sympathetic denervation to the upper extremities during surgery for hyperhidrosis is essential in predicting postoperative outcome, particularly for endoscopic thoracic chain sympathotomy, a recently described, minimally destructive technique that minimizes postoperative compensatory hyperhidrosis. T...

    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章


    authors: Eisenach JH,Pike TL,Wick DE,Dietz NM,Fealey RD,Atkinson JL,Charkoudian N

    更新日期:2005-01-01 00:00:00

  • Erythrocyte fragility screening is not a tool for diagnosis of human malignant hyperthermia.

    abstract::The fragility of erythrocytes from 21 patients undergoing in vitro skeletal muscle contracture testing for malignant hyperthermia (MH) susceptibility was examined. Osmotic fragility was determined by the degree of hemolysis in buffered saline solutions of decreasing osmotic strength. Halothane-induced fragility was de...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Kistler P,Fletcher JE,Rosenberg H

    更新日期:1987-10-01 00:00:00

  • Time required for oral ranitidine to decrease gastric fluid acidity.

    abstract::This study documented gastric fluid acidity in ambulatory surgical patients 30-120 min after they had taken a 300-mg tablet of ranitidine, as changes specific to this relatively brief dose-to-sampling interval previously had not been delineated. At 20-120 min before outpatient surgery, ranitidine was given with 15 mL ...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Jacobs BR,Swift CA,Dubow HD,Kosarussavadi B,McGoldrick K,O'Connor TZ,Silverman DG

    更新日期:1991-12-01 00:00:00

  • Brief report: The sensitivity of motor responses for detecting catheter-nerve contact during ultrasound-guided femoral nerve blocks with stimulating catheters.

    abstract:BACKGROUND:We determined the sensitivity of motor responses evoked by stimulating catheters in determining catheter-nerve contact using ultrasonography as reference. METHODS:Femoral nerves were contacted using stimulating catheters under ultrasonography scanning in 25 patients. The output current was increased from it...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Altermatt FR,Corvetto MA,Venegas C,Echevarría G,Bravo P,De la Cuadra JC,Irribarra LA

    更新日期:2011-11-01 00:00:00

  • A Comparison of Measurements of Change in Respiratory Status in Spontaneously Breathing Volunteers by the ExSpiron Noninvasive Respiratory Volume Monitor Versus the Capnostream Capnometer.

    abstract:BACKGROUND:Current respiratory monitoring technologies such as pulse oximetry and capnography have been insufficient to identify early signs of respiratory compromise in nonintubated patients. Pulse oximetry, when used appropriately, will alert the caregiver to an episode of dangerous hypoxemia. However, desaturation l...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Williams GW 2nd,George CA,Harvey BC,Freeman JE

    更新日期:2017-01-01 00:00:00

  • Oxygen metabolism during liver transplantation: the effect of dichloroacetate.

    abstract:UNLABELLED:Dichloroacetate (DCA) stimulates pyruvate dehydrogenase (PDH), accelerating recovery of the postischemic heart. Because DCA also stimulates hepatic PDH, it may facilitate graft recovery during liver transplantation (OLT). Hepatic removal and replacement during OLT produce major changes in O2 consumption (VO2...

    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Shangraw RE,Robinson ST

    更新日期:1997-10-01 00:00:00

  • Anesthetic considerations for magnetic seizure therapy: a novel therapy for severe depression.

    abstract::Electroconvulsive therapy (ECT) is a highly effective treatment for severe depression. However, its use is associated with significant posttreatment cognitive impairment. Magnetic seizure therapy (MST) was developed as an alternative therapy that could reduce postseizure side effects through the induction of more "foc...

    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章


    authors: White PF,Amos Q,Zhang Y,Stool L,Husain MM,Thornton L,Downing M,McClintock S,Lisanby SH

    更新日期:2006-07-01 00:00:00

  • Dexmedetomidine, an alpha 2-adrenergic agonist, decreases cerebral blood flow in the isoflurane-anesthetized dog.

    abstract::The purpose of this study was to examine the effects of dexmedetomidine, an alpha 2-adrenergic agonist, on cerebral blood flow and metabolic rate in dogs anesthetized with 0.64% isoflurane. After intubation and institution of mechanical ventilation, arterial, venous, pulmonary artery, and sagittal sinus catheters were...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Zornow MH,Fleischer JE,Scheller MS,Nakakimura K,Drummond JC

    更新日期:1990-06-01 00:00:00

  • Differential changes of alveolar gas concentrations during anesthetic induction of a patient with an absent right pulmonary artery.

    abstract::A 38-yr-old man with congenital right pulmonary artery agenesis, whose right lung was perfused with collateral systemic arterial blood, presented for right pneumonectomy. Because of a likely difference in gas exchange between the two lungs, we sampled end-tidal gases from each lung individually, as well as from the co...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Jiang Y,Wain JC,Chang AW,Zapol WM,Xie Z

    更新日期:2006-08-01 00:00:00

  • Isoflurane and halothane produce similar alterations in aortic distensibility and characteristic aortic impedance.

    abstract::The effects of volatile anesthetics on arterial mechanical properties in vivo are incompletely understood. The present investigation tested the hypothesis that isoflurane and halothane cause differential actions on the mechanical characteristics of the aorta in barbiturate-anesthetized dogs instrumented for measuremen...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Hettrick DA,Pagel PS,Warltier DC

    更新日期:1996-12-01 00:00:00

  • Hypotensive effects of adenosine and adenosine triphosphate compared with sodium nitroprusside.

    abstract::Hypotensive effects of the intravenous injection of adenine compounds [adenosine triphosphate (ATP), adenosine] were compared with those of sodium nitroprusside (SNP) in rabbits during light, stable halothane anesthesia. ATP and adenosine were almost equipotent in their effects on blood pressure and heart rate. The hy...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Fukunaga AF,Flacke WE,Bloor BC

    更新日期:1982-03-01 00:00:00

  • Effect of dopamine on hypoxic-hypercapnic interaction in humans.

    abstract::To investigate the effect of intravenous dopamine on the chemical regulation of ventilation, we studied the ventilatory responses to hypercapnic hypoxia during dopamine infusion. Intravenous dopamine (3 micrograms X kg-1 X min-1) was administered to six healthy human subjects. Two hypoxic challenges (PETO2 = 52.5 +/- ...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Sabol SJ,Ward DS

    更新日期:1987-07-01 00:00:00

  • A comparison of lighted stylet (Surch-Lite) and direct laryngoscopic intubation in patients with high Mallampati scores.

    abstract:BACKGROUND:A lighted stylet is an effective alternative to a direct laryngoscope and has been reported to be particularly useful in patients with difficult airways. A high Mallampati class indicates poor visibility of the oropharyngeal structures. Because a lighted stylet does not require direct oropharyngeal visualiza...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章,随机对照试验


    authors: Rhee KY,Lee JR,Kim J,Park S,Kwon WK,Han S

    更新日期:2009-04-01 00:00:00

  • The effects of preoperative inflammation on the analgesic efficacy of intraarticular piroxicam for outpatient knee arthroscopy.

    abstract:UNLABELLED:We conducted a double-blinded study in 90 patients undergoing elective arthroscopic knee surgery to determine whether there is a role of inflammation in the analgesic efficacy of intraarticular piroxicam. Standardized general anesthetic techniques were used for all patients. At the end of the operation, afte...

    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Izdes S,Orhun S,Turanli S,Erkilic E,Kanbak O

    更新日期:2003-10-01 00:00:00

  • Parameters derived from the pulmonary pressure volume curve, but not the pressure time curve, indicate recruitment in experimental lung injury.

    abstract:BACKGROUND:In acute lung injury, ventilation avoiding tidal hyperinflation and tidal recruitment has been proposed to prevent ventilator-associated lung injury. Information about dynamic recruitment may be obtained from the characteristics of pressure-volume (PV) curves or the profile of pressure-time (Paw-t) curves. ...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Henzler D,Hochhausen N,Dembinski R,Orfao S,Rossaint R,Kuhlen R

    更新日期:2007-10-01 00:00:00

  • Excessive variations in the plethysmographic waveform during spontaneous ventilation: an important sign of upper airway obstruction.

    abstract::The respiratory variations in the plethysmographic (PLET) waveform of the pulse oximeter during mechanical ventilation can be automatically quantified as the PLET variation index (PVI(®)). Like other dynamic variables, the PVI may provide useful information about fluid responsiveness but only when the patient is recei...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Perel A

    更新日期:2014-12-01 00:00:00

  • The modified nasal trumpet maneuver.

    abstract:UNLABELLED:The last decade has witnessed a proliferation of devices or methods that facilitate intubation in difficult circumstances, maintain ventilation, or which do both. These all require properly functioning and specially designed apparatus, the use of which requires variable degrees of expertise. This technical c...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Beattie C

    更新日期:2002-02-01 00:00:00

  • Subcellular Energetics and Metabolism: Potential Therapeutic Applications.

    abstract::Part I of this review discussed the similarities between embryogenesis, mammalian adaptions to hypoxia (primarily driven by hypoxia-inducible factor-1 [HIF-1]), ischemia-reperfusion injury (and its relationship with reactive oxygen species), hibernation, diving animals, cancer, and sepsis, and it focused on the common...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章,评审


    authors: Thiele RH

    更新日期:2017-06-01 00:00:00

  • The effect of sevoflurane and isoflurane anesthesia on interictal spike activity among patients with refractory epilepsy.

    abstract:UNLABELLED:The electrophysiologic effects of sevoflurane are not well characterized in humans. Among patients with refractory epilepsy, this study compared 1) electroencephalographic (EEG) interictal spike activity during wakefulness and sevoflurane anesthesia, and 2) electrocorticographically (ECoG) recorded intericta...

    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章


    authors: Watts AD,Herrick IA,McLachlan RS,Craen RA,Gelb AW

    更新日期:1999-11-01 00:00:00

  • Effect of cardiopulmonary bypass on plasma levels of nifedipine.

    abstract::Blood levels of many medications are acutely lowered by cardiopulmonary bypass (CPB). Because nifedipine is often used to provide protection from coronary ischemia, a determination of the effect of CPB on plasma nifedipine levels might help to determine the potential clinical benefit of nifedipine during and after byp...

    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章


    authors: Katz RI,Kanchuger MS,Patton KF,Eide TR

    更新日期:1990-10-01 00:00:00

  • Prevention of hypotension after spinal anesthesia for cesarean section: six percent hetastarch versus lactated Ringer's solution.

    abstract::This study was designed to determine whether preoperative administration of 6% hetastarch decreases the incidence and severity of hypotension after spinal anesthesia for cesarean section. Forty nonlaboring ASA class I and II women having nonurgent cesarean sections were randomized to receive either 500 mL of 6% hetast...

    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Riley ET,Cohen SE,Rubenstein AJ,Flanagan B

    更新日期:1995-10-01 00:00:00