Abstract:
:The effect of Cordyceps militaris culture broth (CMB) on melanogenesis in B16F0 melanoma cells was evaluated by measurement of the melanin concentration after 3 days of incubation. The B16F0 melanoma cells were treated with various concentrations of CMB 10-100 μg/mL and arbutin of 200 μM. Phenolic content and antioxidant activity of CMB were also measured. Phenolic content of CMB was 3.28 mg/g. The DPPH radical scavenging and ferric ion donating activities were 79.64% and 0.16, respectively. The melanin concentration and cell viability of melanoma cells by arbutin treatment decreased to 43% and 91% of the control, respectively. The CMB treatment showed a significant inhibitory effect of melanin production by 29%, 50%, and 56% at 50, 80, and 100 μg/mL concentration treatment, respectively, while over 90% of cells were viable. The CMB treatment at 50, 80, and 100 μg/mL concentrations in cultivation decreased extracellular melanin release induced by 3-isobutyl-1-methylxanthine (IBMX) treatment by 19%, 38%, and 48%, respectively. The CMB showed inhibitory activity against intracellular tyrosinase extracted from melanoma cells, while it had no inhibition on the activity of mushroom tyrosinase. The cellular glutathione contents were enhanced by CMB treatment in a concentration-dependent manner. These results suggested that CMB suppressed cellular tyrosinase activity and total melanin content in cultured B16F0 melanoma cells without any significant effects on cell proliferation and it might be candidate anti-melanogenic agent.
journal_name
Immunopharmacol Immunotoxicoljournal_title
Immunopharmacology and immunotoxicologyauthors
Cha JY,Yang HJ,Park MY,Choi ST,Moon HI,Cho YSdoi
10.3109/08923973.2011.611810subject
Has Abstractpub_date
2011-10-13 00:00:00eissn
0892-3973issn
1532-2513pub_type
杂志文章abstract::The immune effects of acebutolol, a beta-blocker, are investigated in various strains of mice. In 6-12 weeks old C57Bl/6 female mice, an increase in the number of immunoglobulin secreting cells is observed only at the age of 9 weeks. At that age, such an effect is seen neither in C57Bl/6 male mice nor in female mice f...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979009019668
更新日期:1990-01-01 00:00:00
abstract:CONTEXT:Neutrophils are the primary effector cells in the pathogenesis of transfusion-related acute lung injury or multiple organ failure after blood transfusion. OBJECTIVE:We aimed to investigate the effect of fresh (1 day preparation) and aged (42 day preparation) PRBC-derived plasma on neutrophil morphology, migrat...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2012.677046
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Tacrolimus causes post-transplant diabetes mellitus, however the pathogenetic mechanisms remain controversial. In this study we probed into the mechanisms of tacrolimus-induced diabetes mellitus in rats. METHODS:Glucose levels were determined on whole blood samples using a glucose oxidase method. Levels of ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923970903032747
更新日期:2009-01-01 00:00:00
abstract:AIM:To determine the steady state concentration and the pharmacokinetics of Cyclosporine A (CsA) in heart transplant recipients. METHODS:A single dose of 100 mg CsA capsules twice daily was given to five heart transplant recipients of steady state. The concentrations of CsA in plasma were determined by streptavidin pe...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 临床试验,杂志文章
doi:10.3109/08923973.2011.613400
更新日期:2012-06-01 00:00:00
abstract::The rate of collagen deposition in implanted polytetrafluoroethylene (PTFE) tubing in non-diabetic and streptozotocin-induced (STZ) diabetic mice was measured during 14 days post-wounding. At the time of implantation, test groups received injections of either Quadrol [N,N,N',N'-tetrakis(2-hydroxypropyl)ethylenediamine...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923978809006452
更新日期:1988-01-01 00:00:00
abstract::The anti-tumour antibiotic Bleomycin, the experimental immunostimulatory tripeptide FK 565, and the immunosuppressive agent Cyclosporin A were examined for their in vitro effects on the release of the haemopoietic cytokine Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) from LPS stimulated BALB/c adherent pe...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979509016381
更新日期:1995-08-01 00:00:00
abstract::Immunomodulatory activity of Nelumbo nucifera rhizome was evaluated for its standardized extract (NNRE) with respect to betulinic acid. Various key parameters including erythrocyte membrane stabilization, inhibition of histamine release, reduction in nitric oxide production and depletion of expression of costimulatory...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923970903514830
更新日期:2010-09-01 00:00:00
abstract::Objectives: Several studies demonstrated the antioxidant and anti-inflammatory role of melatonin and celecoxib. This study is designed to explore the underlying mechanism of hepatoprotective effects of melatonin and celecoxib against ethanol-induced hepatotoxicity by morphological, and biochemical approaches.Materials...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923973.2020.1746802
更新日期:2020-06-01 00:00:00
abstract::Immunostimulants known to initiate cytokine production were found to inhibit processes of microsomal drug oxidation but to activate arylamine N-acetylation. The present study investigated the effects of immunstimulating doses of rat interferon-gamma (IFN gamma, 670,000 units ip) and streptolysin O (SLO, 100 HU/kg iv f...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979609052754
更新日期:1996-11-01 00:00:00
abstract::We conducted a comparative study of NK1.1+ cells in spleen and bone marrow and the effects of administration of M-CSF on them. Administration of M-CSF to mice increased the number of NK1.1+ cells in spleen but not in bone marrow. The NK1.1+ cells in spleen (Spl-NK1.1) and bone marrow (BM-NK1.1) were purified by magnet...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120016035
更新日期:2002-11-01 00:00:00
abstract::We examined the effect of sulfapyridine on mast cell-mediated immediate-type allergic reactions. Sulfapyridine (1 and 10 microg/kg) significantly inhibited systemic allergic reaction induced by compound 48/80 in rats. Sulfapyridine (1 and 10 microg/kg) also inhibited significantly local mast cell-mediated immediate-ty...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923970009016419
更新日期:2000-05-01 00:00:00
abstract::The induction of T-cell responses involves the recognition of extrinsic antigens in association with antigens of the major histocompatibility complex (MHC). The present results demonstrate that the lateralized control exerted by the brain neocortex on T-cell activities extends to the expression of MHC antigens, yet di...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923978809014334
更新日期:1988-01-01 00:00:00
abstract::The effects of cimetidine upon suppressor cell effector function in a well-studied murine model of contact hypersensitivity were examined. Intravenous inoculation of BALB/c mice with DNP-coupled syngeneic spleen cells induced the production of DNP-specific suppressor cells which was demonstrated by a reduction in ear ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923978809041424
更新日期:1988-01-01 00:00:00
abstract::The protective effects of baicalin (BA), a major flavone from Scutellaria radix, on acetaminophen (AP)-induced hepatotoxicity and the possible mechanism(s) of its protective action were investigated in mice. Treatment with BA (300 mg/kg, p.o.) 0.5 h after AP administration significantly prevented an increase in plasma...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120026443
更新日期:2003-11-01 00:00:00
abstract::Taraxasterol, a pentacyclic-triterpene, was isolated from the Chinese medicinal herb Taraxacum officinale. In the present study, we investigated the protective effect of taraxasterol on murine model of endotoxic shock and the mechanism of its action. Mice were treated with 2.5, 5 and 10 mg/kg of taraxasterol prior to ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2013.861482
更新日期:2014-02-01 00:00:00
abstract::Chronic administration of oxytetracycline (OXT) (incorporated at a level of 0.05 g per kg of feed for 50 days) to pigeons, significantly decreased total leukocyte and lymphocyte counts, increased heterophil:lymphocyte ratio and lysosomal enzyme activity, and decreased reticuloendothelial system function compared with ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970500242327
更新日期:2005-01-01 00:00:00
abstract::Spleen and thymus weight, delayed-type hypersensitivity (DTH) to sheep red blood cells (SRBC), contact hypersensitivity to picryl chloride, anti-sheep red blood cell hemagglutinin titers and plaque-forming cells (PFC), resistance against experimental toxoplasmosis as well as serum total and intestinal specific IgA wer...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923978909005388
更新日期:1989-01-01 00:00:00
abstract::It has been shown that either cocaine or aging alone can alter the immune system. Our objective was to study if the immune system of aging mice was more susceptible to the effect of cocaine than the immune system of young mice. We used a short term (20 days) cocaine daily administration protocol. Cocaine only decrease...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979909007140
更新日期:1999-11-01 00:00:00
abstract::We have previously shown that a mitogenic fraction (PH-I) separated from Pseudostellaria heterophylla (P. heterophylla) could act as a priming agent for the release of tumor necrosis factor-alpha (TNF-alpha) in mice. In the present study, PH-I was further purified by gel filtration chromatography and the resulting thr...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979409007098
更新日期:1994-08-01 00:00:00
abstract::Myricetin, a common dietary flavonoid, is widely distributed in fruits and vegetables. It is known to be a food supplement contributing to human health because of its immune modulatory function, and its antioxidation, antitumor, and anti-inflammatory properties. In the present study, myricetin was shown to directly in...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2015.1096942
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND AND AIM:To probe into the effects of different doses of FK506 on endocrine function of pancreatic islets in Wistar rats. METHODS:Wistar rats were randomly divide into control group A (0mg/kg/d) and experimental group B (1mg/kg/d), C (3mg/kg/d), D (10mg/kg/d) treated with FK506. In an intraperitoneal glucose...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923970903358191
更新日期:2010-06-01 00:00:00
abstract::Phenolic composition and antioxidant activities of the aqueous extract of Arisaema cum Bile, which is widely used as a folk medicine in Korea, were determined. Phenolic composition profile revealed that the aqueous extract is rich in sinapic acid (13.14 mg/100 g extract), catechin (9.88 mg/100 g extract), neohesperidi...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2011.649289
更新日期:2012-08-01 00:00:00
abstract::Contribution of cytotoxic T lymphocytes (CTL) to experimental autoimmune thyroiditis (EAT) was well defined (Speidel et al., Eur. J. Immunol. 1997, 27, 2391-2399, Ref. 7). The native porcine thyroglobulin (pTg) showed high sensitivity to endo-o-N-acetylglucosaminidase F (Endo F) and its molecular weights, correspondin...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章,收录出版
doi:10.1081/iph-200026872
更新日期:2004-08-01 00:00:00
abstract::Two dideoxynucleosides, 2',3'-dideoxy-beta-L-cytidine and 2',3'-dideoxy-beta-L-5-flurocytidine, containing unnatural L-configuration in their sugar moieties, were synthesized and assayed for antiviral activities. Both compounds were shown to possess potent anti-human immunodeficiency virus type 1 and antihepatitis B v...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979509052717
更新日期:1995-02-01 00:00:00
abstract::Although investigations to date have demonstrated the ability of the monocyte/macrophage to synthesize complement components, only a limited number of studies on complement synthesis by nonhepatic tissue cells have been reported. To begin to fill this gap in our knowledge we have recently evaluated the ability of lung...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979409019738
更新日期:1994-11-01 00:00:00
abstract::Atractylenolide I (ATL-I) is a bioactive component of Rhizoma Atractylodis macrocephalae. Although increasing evidence shows that ATL-I has an anti-inflammatory effect, the anti-inflammatory molecular mechanism of ATL-I is still unknown. In this study, we investigated the effect of ATL-I on cell viability by 3-(4, 5-D...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2014.968256
更新日期:2014-12-01 00:00:00
abstract::The purpose of our study was to compare the effectiveness of immunosuppressive drugs on the prevention of allograft rejection in a murine model of low-risk and high-risk keratoplasty. The therapy included FK 506 (tacrolimus; 0.2 mg/kg), mycophenolate mofetil (30 mg/kg), aminoguanidine (0.1 g/kg), and combination of FK...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970600809447
更新日期:2006-01-01 00:00:00
abstract::A human hepatoma cell line, Hep G2 cells are reliable for the study of alcohol-induced hepatotoxicity. In this study, we investigated the effect of an aqueous extract of Polygala tenuifolia WILLDENOW (Polygalaceae) roots (PTAE) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. PTAE (0.01-1 microg/ml) dose-depend...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923970009026010
更新日期:2000-08-01 00:00:00
abstract::We have previously found that NK cell activity is present in rat lung lavage and inhibited by lidocaine. The purpose of this report is to characterize further the inhibition by lidocaine on NK activity. Peripheral blood lymphocytes from normal human volunteers were assayed in a four-hour 51chromium release assay in th...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979009006471
更新日期:1990-01-01 00:00:00
abstract::Several abnormalities of the immune system have been reported in association with clinical and experimental iron overload. To dissect further such abnormalities, changes in lymphocyte subsets were evaluated in iron-loaded male Sprague-Dawley rats. The iron-loading protocol consisted of a total dose of irondextran (1.5...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979709038534
更新日期:1997-02-01 00:00:00