Melanogenesis effect of Cordyceps militaris culture broth on the melanin formation of B16F0 melanoma cells.


:The effect of Cordyceps militaris culture broth (CMB) on melanogenesis in B16F0 melanoma cells was evaluated by measurement of the melanin concentration after 3 days of incubation. The B16F0 melanoma cells were treated with various concentrations of CMB 10-100 μg/mL and arbutin of 200 μM. Phenolic content and antioxidant activity of CMB were also measured. Phenolic content of CMB was 3.28 mg/g. The DPPH radical scavenging and ferric ion donating activities were 79.64% and 0.16, respectively. The melanin concentration and cell viability of melanoma cells by arbutin treatment decreased to 43% and 91% of the control, respectively. The CMB treatment showed a significant inhibitory effect of melanin production by 29%, 50%, and 56% at 50, 80, and 100 μg/mL concentration treatment, respectively, while over 90% of cells were viable. The CMB treatment at 50, 80, and 100 μg/mL concentrations in cultivation decreased extracellular melanin release induced by 3-isobutyl-1-methylxanthine (IBMX) treatment by 19%, 38%, and 48%, respectively. The CMB showed inhibitory activity against intracellular tyrosinase extracted from melanoma cells, while it had no inhibition on the activity of mushroom tyrosinase. The cellular glutathione contents were enhanced by CMB treatment in a concentration-dependent manner. These results suggested that CMB suppressed cellular tyrosinase activity and total melanin content in cultured B16F0 melanoma cells without any significant effects on cell proliferation and it might be candidate anti-melanogenic agent.


Cha JY,Yang HJ,Park MY,Choi ST,Moon HI,Cho YS




Has Abstract


2011-10-13 00:00:00






  • A recombinant polypeptide from velvet antler of Cervus nippon Temminck exhibits similar immunomodulatory effects as its natural counterpart.

    abstract:CONTEXT:Velvet antler (VA) is recognized as one of the most important Chinese traditional medical herbs. To date, the immunoactivity of the single component of VA is rarely studied though its compound extracts have been well analyzed. OBJECTIVE:The current study was designed to evaluate the immunomodulatory effects of...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Zha E,Dandan L,Bai X,Zhou T,Li Y,Shenyang G,Yue X

    更新日期:2016-12-01 00:00:00

  • Pulmonary TCR γδ T cells induce the early inflammation of granuloma formation by a glycolipid trehalose 6,6'-dimycolate (TDM) isolated from Mycobacterium tuberculosis.

    abstract::We previously showed that formation of pulmonary granulomas in mice in response to a mycobacterial glycolipid, trehalose 6,6'-dimycolate (TDM) is due to the action of TNF-α and not of IFN-γ. However, the mechanisms of formation and maintenance of pulmonary granulomas are not yet clear. The purpose of the present study...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Saitoh T,Yano I,Kumazawa Y,Takimoto H

    更新日期:2012-10-01 00:00:00

  • Ex vivo triggering of T-cell-mediated immune responses by autologous tumor cell vaccine in oral cancer patients.

    abstract::We investigated immunomodulatory activity of autologous tumor cell vaccine from oral cancer patients ex vivo by lymphoproliferation assay and two color flow cytometry. Vaccine treatment lead to 10-fold higher proliferation of lymphocytes compared with the untreated controls. A significant increase in CD69(+) and HLA-D...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 临床试验,杂志文章


    authors: Agarwal A,Mohanti BK,Das SN

    更新日期:2007-01-01 00:00:00

  • Tubeimoside-1 inhibits the proliferation and activation of mouse T lymphocytes through signal transduction pathways.

    abstract::Tubeimoside-1 (TBMS1) is one of the important components in Bolbostemma paniculatum (Maxim.) Franque. In the study, its immunosuppressive effects on murine T lymphocyte responses were evaluated in vitro and in vivo. The data showed that TBMS1 inhibited ConA-induced T lymphocyte proliferation, decreased the ratio of CD...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Huang Y,Ren M,Xiong Y,Wang T,Chen H,Deng X,Lu J

    更新日期:2015-01-01 00:00:00

  • A possible role to nitric oxide in the anti-inflammatory effects of amitriptyline.

    abstract::Antidepressants are reported to display anti-inflammatory effects. Nitric oxide (NO), in turn, has a key role in inflammation. The objective of the present study was to evaluate the effect of amitriptyline co-administered with L-NAME (a NO synthase inhibitor) on certain parameters of acute inflammatory response in rat...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Vismari L,Alves GJ,Muscará MN,Palermo-Neto J

    更新日期:2012-08-01 00:00:00

  • Sorafenib in hepatocellular carcinoma - a post marketing evaluation.

    abstract:BACKGROUND:Sorafenib is an orally active multikinase inhibitor licensed for the treatment of patients with unresectable hepatocellular carcinoma (HCC). PATIENTS AND METHODS:The web-based registry, used for appraisal on new drugs, allows developing the observational prospective analysis of innovative drug therapies. To...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Trojniak MP,Palozzo AC,Mazurek M,Jirillo A

    更新日期:2012-06-01 00:00:00

  • Effect of short-term cocaine administration on the immune system of young and old C57BL/6 female mice.

    abstract::It has been shown that either cocaine or aging alone can alter the immune system. Our objective was to study if the immune system of aging mice was more susceptible to the effect of cocaine than the immune system of young mice. We used a short term (20 days) cocaine daily administration protocol. Cocaine only decrease...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Colombo LL,López MC,Chen GJ,Watson RR

    更新日期:1999-11-01 00:00:00

  • Immunomodulating effects of black seed and oxytetracycline in pigeons.

    abstract::Chronic administration of oxytetracycline (OXT) (incorporated at a level of 0.05 g per kg of feed for 50 days) to pigeons, significantly decreased total leukocyte and lymphocyte counts, increased heterophil:lymphocyte ratio and lysosomal enzyme activity, and decreased reticuloendothelial system function compared with ...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Al-Ankari AS

    更新日期:2005-01-01 00:00:00

  • Tolerance test in patients with multiple drug allergy syndrome.

    abstract::Multiple Drug Allergy Syndrome (MDAS) is a frequent clinical condition characterized by reactions to more than one different class of antibiotics. Even if some studies have previously reported an increased rate of allergic reactions to drugs in patients with a history of antimicrobials and NSAIDs allergy, risk factors...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Nettis E,Colanardi MC,Paola RD,Ferrannini A,Tursi A

    更新日期:2001-11-01 00:00:00

  • The effects of cadmium exposure in the induction of inflammation.

    abstract::Inflammation is a physiological process essential for maintaining homeostatic mechanisms in human, but however, exaggerated inflammatory responses are closely related to many chronic diseases. Cadmium (Cd) is a heavy metal with high toxicity when present in food, water and air has the potential of eliciting inflammato...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章,评审


    authors: Hossein-Khannazer N,Azizi G,Eslami S,Alhassan Mohammed H,Fayyaz F,Hosseinzadeh R,Usman AB,Kamali AN,Mohammadi H,Jadidi-Niaragh F,Dehghanifard E,Noorisepehr M

    更新日期:2020-02-01 00:00:00

  • Evaluation of anti-inflammatory, anti-nociceptive, and anti-arthritic activities of Indian Apis dorsata bee venom in experimental animals: biochemical, histological, and radiological assessment.

    abstract::Traditionally venoms are used from thousands of years to treat pain, inflammation, and arthritis. In Ayurveda "Suchika Voron" and "Shodhona" were practiced against pain. In the present study, venom composition of the Indian honeybee Apis florea (AF), Apis dorsata (AD), and Apis cerana indica (AC) were analyzed using e...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Nipate SS,Hurali PB,Ghaisas MM

    更新日期:2015-04-01 00:00:00

  • Scrophularia buergeriana regulates cytokine production in vitro.

    abstract::The Scrophularia buergeriana (SB) has long been used to treat various diseases an account of its antimicrobial and anti-virus activity. However, it is unclear how SB regulates the immune responses. This study investigated the effect of SB on the production of cytokines in a human T-cell line, MOLT-4 cells, and mouse p...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Kim SJ,Park JS,Myung NY,Moon PD,Choi IY,An HJ,Kim NH,Na HJ,Kim DH,Kim MC,An NH,Kim IK,Lee JY,Jeong HJ,Um JY,Kim HM,Hong SH

    更新日期:2009-06-01 00:00:00

  • A copper chelate selectively triggers apoptosis in myeloid-derived suppressor cells in a drug-resistant tumor model and enhances antitumor immune response.

    abstract::Myeloid-derived suppressor cells (MDSCs), one of the major orchestrators of immunosuppressive network are present in the tumor microenvironment suppress antitumor immunity by subverting Th1 response in tumor site and considered as a great obstacle for advancement of different cancer immunotherapeutic protocols. Till d...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Chakraborty P,Das S,Banerjee K,Sinha A,Roy S,Chatterjee M,Choudhuri SK

    更新日期:2014-04-01 00:00:00

  • Inhibition of tumor cells growth and stimulation of lymphocytes by Euphorbia species.

    abstract::Plants have been shown to possess a number of beneficial anticancer and immunomodulatory properties. In this study the possible in vitro antitumor activity and immunomodulatory effects of five species of Euphorbia, an important genus of Euphorbiaceae, including E. petiolata, E. hebecarpa, E. osyridea, E. microciadia, ...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Amirghofran Z,Malek-hosseini S,Gholmoghaddam H,Kalalinia F

    更新日期:2011-03-01 00:00:00

  • Novel effects of On-Chung-Eum, the traditional plant medicine, on cytokine production in human mononuclear cells from Behçet's.

    abstract::Plant medications have been used as treatment in various kinds of systemic inflammatory disorder such as Behçet's disease (BD). We investigated the roles of On-Chung-Eum (OCE), a traditional plant medicine, in cytokine regulation of BD. The effects of OCE on cytokine production from phytohaemagglutinin (PHA)-stimulate...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Kim MS,Kim SO,Lim WK,Lee JM,Hwang CY,Cho KH,Kim HM

    更新日期:2003-02-01 00:00:00

  • HP-NAP ameliorates OXA-induced atopic dermatitis symptoms in mice.

    abstract:BACKGROUND:Atopic dermatitis (AD) is a chronic skin inflammatory disease characterized by disequilibrium between Th1/Th2 lymphocytes. Helicobacter pylori neutrophil-activating protein (HP-NAP) has been reported that it has the potential immunomodulatory effect able to regulate the Th1/Th2 balance. OBJECTIVE:This study...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Guo X,Ding C,Lu J,Zhou T,Liang T,Ji Z,Xie P,Liu X,Kang Q

    更新日期:2020-10-01 00:00:00

  • Preclinical assessment of β-d-mannuronic acid (M2000) as a non-steroidal anti-inflammatory drug.

    abstract:CONTEXT:β-d-Mannuronic acid (M2000) has shown its therapeutic effects with the greatest tolerability and efficacy in various experimental models such as experimental autoimmune encephalomyelitis (EAE), adjuvant induced arthritis (AIA), nephrotic syndrome, and acute glomerulonephritis. Despite pharmacological effects of...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Fattahi MJ,Abdollahi M,Agha Mohammadi A,Rastkari N,Khorasani R,Ahmadi H,Tofighi Zavareh F,Sedaghat R,Tabrizian N,Mirshafiey A

    更新日期:2015-01-01 00:00:00

  • Siglec-8 and Siglec-F, the new therapeutic targets in asthma.

    abstract::The recruitment of eosinophils from the circulation into the airway is a prominent feature of allergic asthma. Persistent inflammatory responses may arise from inefficient mechanisms for resolution of inflammation, including delayed apoptosis. Several studies suggest that eosinophil apoptosis is delayed in asthma. Sia...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章,评审


    authors: Farid SSh,Mirshafiey A,Razavi A

    更新日期:2012-10-01 00:00:00

  • Induction of a TC1-mediated experimental autoimmune thyroiditis by deglycosylated porcine thyroglobulin.

    abstract::Contribution of cytotoxic T lymphocytes (CTL) to experimental autoimmune thyroiditis (EAT) was well defined (Speidel et al., Eur. J. Immunol. 1997, 27, 2391-2399, Ref. 7). The native porcine thyroglobulin (pTg) showed high sensitivity to endo-o-N-acetylglucosaminidase F (Endo F) and its molecular weights, correspondin...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章,收录出版


    authors: Kang BS,Chung TW,Moon JY,Lim JK,Shon YH,Nam KS,Kim DS,Jeon BH,Kim CH

    更新日期:2004-08-01 00:00:00

  • Comparative study and effects of macrophage colony-stimulating factor (M-CSF) administration on NK1.1+ cells in mouse spleen and bone marrow.

    abstract::We conducted a comparative study of NK1.1+ cells in spleen and bone marrow and the effects of administration of M-CSF on them. Administration of M-CSF to mice increased the number of NK1.1+ cells in spleen but not in bone marrow. The NK1.1+ cells in spleen (Spl-NK1.1) and bone marrow (BM-NK1.1) were purified by magnet...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Sakurai T,Misawa E,Yamada M,Hayasawa H,Motoyoshi K

    更新日期:2002-11-01 00:00:00

  • 17beta-Estradiol increases the number of effector memory CD8+ lymphocytes in mice with contact hypersensitivity and among cultured splenocytes.

    abstract::To determine how estrogen exacerbates allergies, the effects of 17beta-estradiol (E2) on lymphocyte proliferation were investigated. BALB/c mice were ovariectomized, administered 3.2 microg E2, and sensitized with 50 microL 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one. After 7 days, their spleens were excised and flow ...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Sakazaki F,Ueno H,Nakamuro K

    更新日期:2010-06-01 00:00:00

  • Quantitative determination of pegylated consensus interferon in rhesus monkey serum using a competitive enzyme-linked immunosorbent assay.

    abstract::A competitive enzyme-linked immunosorbent assay (ELISA) with generated mouse anti-consensus interferon (CIFN) antibody was developed for quantitative determination of pegylated consensus interferon (PEG-CIFN) in rhesus monkey serum. The operational concentrations of the original assay were determined using the chessbo...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Cao J,Du Y,Tian H,Gao XD,Yao WB

    更新日期:2009-01-01 00:00:00

  • Immunological effects following administration of interferon-alpha in patients with chronic hepatitis C virus (cHCV) infection.

    abstract::The immunological effects of interferon (IFN)-alpha administration were evaluated in 15 patients with cHCV infection. Individuals were treated with 6 MU of lymphoblastoid IFN-alpha three times a week for 6 months and with 3 MU three times a week for an additional 6 months. Patients were divided into responders (12 sub...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Jirillo E,Greco B,Caradonna L,Satalino R,Amati L,Cozzolongo R,Cuppone R,Manghisi OG

    更新日期:1996-08-01 00:00:00

  • Immunomodulatory activity of a novel nucleoside, 7-thia-8-oxoguanosine: I. Activation of natural killer cells in mice.

    abstract::We reported recently that a novel immunomodulator, 7-thia-8-oxoguanosine (7T8OG)2 inhibited formation of pulmonary melanoma metastases (1), prevented against viral infection in mice (2) and potentiated the efficacy of a weakly immunogenic leukemia vaccine (3). Since certain tumor metastases and virus infected cells ar...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Sharma BS,Mhaskar S,Balazs L,Siaw M

    更新日期:1992-01-01 00:00:00

  • Polyclonal stimulation of lymphocytes induced by beta blockers in the mouse: influences of age, sex and strain.

    abstract::The immune effects of acebutolol, a beta-blocker, are investigated in various strains of mice. In 6-12 weeks old C57Bl/6 female mice, an increase in the number of immunoglobulin secreting cells is observed only at the age of 9 weeks. At that age, such an effect is seen neither in C57Bl/6 male mice nor in female mice f...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: In S,Lulé J,Fournié GJ

    更新日期:1990-01-01 00:00:00

  • Functional changes in THP-1 human monocytic cells after stimulation with lipopolysaccharide of oral microorganisms and granulocyte macrophage colony stimulating factor.

    abstract::A human THP-1 monocyte cell line culture system has been utilized to observe the effect of granulocyte macrophage colony stimulating factor (GM-CSF) supplementation with lipopolysaccharide (LPS) of oral microorganisms to stimulate monocyte/macrophage functional activity. LPS of oral microorganisms, Fusobacterium nucle...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Baqui AA,Meiller TF,Turng BF,Kelley JI,Falkler WA Jr

    更新日期:1998-11-01 00:00:00

  • Immunomodulatory effect of the homoeopathic drug Engystol-N on some activities of isolated human leukocytes and in whole blood.

    abstract::Engystol-N at the doses of 10(-4) and 10(-8) in isolated human leukocytes stimulates the superoxide anion generation by neutrophils and the cytokine(s) production by T lymphocytes. In whole blood the same concentrations of the drug produce the decrease of the superoxide anion generation of neutrophils, this inhibiting...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Fimiani V,Cavallaro A,Ainis O,Bottari C

    更新日期:2000-02-01 00:00:00

  • Artesunate enhances γδ T-cell-mediated antitumor activity through augmenting γδ T-cell function and reversing immune escape of HepG2 cells.

    abstract:OBJECTIVE:To explore the effect and mechanism of artesunate on γδ T cell-mediated antitumor immune responses against hepatoma carcinoma cells (HepG2) in vitro. METHODS:Human γδ T cells or HepG2 were respectively treated with artesunate, subjected to co-culture as appropriate, and the following assays were subsequently...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Qian P,Zhang YW,Zhou ZH,Liu JQ,Yue SY,Guo XL,Sun LQ,Lv XT,Chen JQ

    更新日期:2018-04-01 00:00:00

  • Chlorphentermine suppresses the phosphatidylinositol pathway in concanavalin A-activated mouse splenic lymphocytes.

    abstract::We have previously demonstrated that the chlorphentermine (CP)1-induced impairment in lymphocyte blastogenesis involves drug-induced inhibition of an event which occurs very early during lymphocyte activation. An early event, which is associated with mitogen-induced lymphocyte activation, involves the hydrolysis of ph...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Sauers LJ,Wierda D,Reasor MJ

    更新日期:1988-01-01 00:00:00

  • Immunomodulatory and antiviral activities of 2',3'-dideoxy-beta-L-cytidine and 2',3'-dideoxy-beta-L-5-fluorocytidine.

    abstract::Two dideoxynucleosides, 2',3'-dideoxy-beta-L-cytidine and 2',3'-dideoxy-beta-L-5-flurocytidine, containing unnatural L-configuration in their sugar moieties, were synthesized and assayed for antiviral activities. Both compounds were shown to possess potent anti-human immunodeficiency virus type 1 and antihepatitis B v...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章


    authors: Gagnon L,Nordstrom PA,Duchaine J,Jutras D,Hamel M,Barbeau D,Hooker E,Ashman C,Cammack N,Tse A

    更新日期:1995-02-01 00:00:00