Abstract:
:The effect of Cordyceps militaris culture broth (CMB) on melanogenesis in B16F0 melanoma cells was evaluated by measurement of the melanin concentration after 3 days of incubation. The B16F0 melanoma cells were treated with various concentrations of CMB 10-100 μg/mL and arbutin of 200 μM. Phenolic content and antioxidant activity of CMB were also measured. Phenolic content of CMB was 3.28 mg/g. The DPPH radical scavenging and ferric ion donating activities were 79.64% and 0.16, respectively. The melanin concentration and cell viability of melanoma cells by arbutin treatment decreased to 43% and 91% of the control, respectively. The CMB treatment showed a significant inhibitory effect of melanin production by 29%, 50%, and 56% at 50, 80, and 100 μg/mL concentration treatment, respectively, while over 90% of cells were viable. The CMB treatment at 50, 80, and 100 μg/mL concentrations in cultivation decreased extracellular melanin release induced by 3-isobutyl-1-methylxanthine (IBMX) treatment by 19%, 38%, and 48%, respectively. The CMB showed inhibitory activity against intracellular tyrosinase extracted from melanoma cells, while it had no inhibition on the activity of mushroom tyrosinase. The cellular glutathione contents were enhanced by CMB treatment in a concentration-dependent manner. These results suggested that CMB suppressed cellular tyrosinase activity and total melanin content in cultured B16F0 melanoma cells without any significant effects on cell proliferation and it might be candidate anti-melanogenic agent.
journal_name
Immunopharmacol Immunotoxicoljournal_title
Immunopharmacology and immunotoxicologyauthors
Cha JY,Yang HJ,Park MY,Choi ST,Moon HI,Cho YSdoi
10.3109/08923973.2011.611810subject
Has Abstractpub_date
2011-10-13 00:00:00eissn
0892-3973issn
1532-2513pub_type
杂志文章abstract::Plant medications have been used as treatment in various kinds of systemic inflammatory disorder such as Behçet's disease (BD). We investigated the roles of On-Chung-Eum (OCE), a traditional plant medicine, in cytokine regulation of BD. The effects of OCE on cytokine production from phytohaemagglutinin (PHA)-stimulate...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120018284
更新日期:2003-02-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) is a chronic skin inflammatory disease characterized by disequilibrium between Th1/Th2 lymphocytes. Helicobacter pylori neutrophil-activating protein (HP-NAP) has been reported that it has the potential immunomodulatory effect able to regulate the Th1/Th2 balance. OBJECTIVE:This study...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923973.2020.1806869
更新日期:2020-10-01 00:00:00
abstract::Rosa davurica Pall. has been traditionally used to treat inflammatory diseases and tumors. Its dried leaves were extracted with absolute methanol, and the methanol extract was successively fractionated into n-hexane, chloroform, ethyl acetate, n-butanol, and aqueous fractions. Anti-angiogenic and anti-nociceptive acti...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2010.491516
更新日期:2011-03-01 00:00:00
abstract::A human hepatoma cell line, Hep G2 cells are reliable for the study of alcohol-induced hepatotoxicity. In this study, we investigated the effect of an aqueous extract of Polygala tenuifolia WILLDENOW (Polygalaceae) roots (PTAE) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. PTAE (0.01-1 microg/ml) dose-depend...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923970009026010
更新日期:2000-08-01 00:00:00
abstract::We investigated immunomodulatory activity of autologous tumor cell vaccine from oral cancer patients ex vivo by lymphoproliferation assay and two color flow cytometry. Vaccine treatment lead to 10-fold higher proliferation of lymphocytes compared with the untreated controls. A significant increase in CD69(+) and HLA-D...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 临床试验,杂志文章
doi:10.1080/08923970701282742
更新日期:2007-01-01 00:00:00
abstract::Recently, a number of studies have focused on the secondary effects of rifampicin. In the present study, we assessed whether rifampicin influences the rapamycin-induced autophagy of RAW 264.7 cells. Here, we demonstrate that the rapamycin-induced autophagy is dependent on protein phosphatase (PP) 2A activity and rifam...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970802135732
更新日期:2008-01-01 00:00:00
abstract::Luminol-enhanced chemiluminescence assay was used to detect the surface expression and the consequent activation of receptors (FcRI and FcRII) of murine macrophages (M phi s). When murine IgG2a was used for the specific detection of FcRI and IgG2b for FcRII, a newly established procedure enabled us to detect the activ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923978909005371
更新日期:1989-01-01 00:00:00
abstract::We studied the effect of pyridoxine deficiency state on the responses of human lymphocytes to certain mitogenic factors. Deoxypyridoxine (DB6), a potent pyridoxine antagonist, considerably inhibited Bromodeoxyuridine (BrdU) incorporation into the newly synthesized DNA of lymphocytes cultured with Phytohemagglutinin (P...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923978909005393
更新日期:1989-01-01 00:00:00
abstract:CONTEXT:Foeniculum vulgare (F. vulgare) is traditionally used to treat inflammatory diseases. Recently, anti-inflammatory and antioxidant activities of methanol extract of the fruits of F. vulgare were reported. To identify biologically active compounds responsible for the anti-inflammatory activity, we isolated four c...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2015.1038751
更新日期:2015-06-01 00:00:00
abstract::Present inventory evaluates the anti-atherogenic potential of C. glandulosum.Coleb leaf extract (CG) using in vivo and in vitro experimental models. Serum markers of low density lipoprotein (LDL-C) oxidation, cholesterol, triglycerides, lipoproteins, auto-antibody titer, ex vivo LDL-C oxidation, LDL-C aggregation, aor...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2011.618136
更新日期:2012-06-01 00:00:00
abstract::Plants have been shown to possess a number of beneficial anticancer and immunomodulatory properties. In this study the possible in vitro antitumor activity and immunomodulatory effects of five species of Euphorbia, an important genus of Euphorbiaceae, including E. petiolata, E. hebecarpa, E. osyridea, E. microciadia, ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923971003699018
更新日期:2011-03-01 00:00:00
abstract::We investigated the effect of aqueous extract of Sanguisorba officinalis L.(Rosaceae) root (SOAE) on the immediate-type allergic reactions in vivo and in vitro. SOAE (0.01 to 1 g/kg) inhibited systemic allergic reaction induced by compound 48/80. When SOAE was employed in a systemic allergic reaction test, the plasma ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120014729
更新日期:2002-08-01 00:00:00
abstract::Chronic administration of oxytetracycline (OXT) (incorporated at a level of 0.05 g per kg of feed for 50 days) to pigeons, significantly decreased total leukocyte and lymphocyte counts, increased heterophil:lymphocyte ratio and lysosomal enzyme activity, and decreased reticuloendothelial system function compared with ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970500242327
更新日期:2005-01-01 00:00:00
abstract::The current research was designed to determine the effect of artemether in treatment of experimental rheumatoid arthritis. Collagen-induced arthritis was induced in Lewis rats. The intramusculary administration of artemether (ART) and intraperitoneally injection of methotrexate (MTX) were started on day 25 postimmuniz...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970500418786
更新日期:2005-01-01 00:00:00
abstract::We have developed an in vitro system to model the interactions of drugs used to treat transplant rejection. This system consists of stimulation of human lymphocytes with a primary mitogen (anti-T-cell receptor complex antibodies (OKT3 or wt31)) and treatment with a primary immunosuppressive drug (ISD) (Cyclosporine A ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979409007089
更新日期:1994-05-01 00:00:00
abstract::Spleen and thymus weight, delayed-type hypersensitivity (DTH) to sheep red blood cells (SRBC), contact hypersensitivity to picryl chloride, anti-sheep red blood cell hemagglutinin titers and plaque-forming cells (PFC), resistance against experimental toxoplasmosis as well as serum total and intestinal specific IgA wer...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923978909005388
更新日期:1989-01-01 00:00:00
abstract::Association between Crohn's disease (CD) and lupus nephritis is very rare and, to the best of our knowledge, it has been described only once. We report here a clinical case of CD occurred in a young woman 8 years after a diagnosis of lupus nephritis according to clinical, laboratory and histological criteria. CD was u...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120037721
更新日期:2004-05-01 00:00:00
abstract::Serum tumor necrosis factor (TNF) levels in 33 patients with inflammatory bowel disease (IBD) were measured by using a sensitive enzyme immunoassay. Four of five Crohn's diseases (CD) and nine of twenty eight ulcerative colitis (UC) had elevated levels of serum TNF. In active CD or UC, a greater fraction of patients s...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979209005404
更新日期:1992-01-01 00:00:00
abstract::Traditionally venoms are used from thousands of years to treat pain, inflammation, and arthritis. In Ayurveda "Suchika Voron" and "Shodhona" were practiced against pain. In the present study, venom composition of the Indian honeybee Apis florea (AF), Apis dorsata (AD), and Apis cerana indica (AC) were analyzed using e...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2015.1009996
更新日期:2015-04-01 00:00:00
abstract::Myasthenia gravis is an autoimmune disease in which antibodies are produced against one's acetylcholine receptors, resulting in complement-mediated membrane destruction and internalization of antibody-receptor complexes. Symptoms range from weakening of extraocular muscles to severe impairment of movement and breathin...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979809038542
更新日期:1998-05-01 00:00:00
abstract:CONTEXT:To develop a personalized peptide vaccine (PPV) for recurrent ovarian cancer patients and evaluate its efficacy from the point of view of overall survival (OS), Phase II study of PPV was performed. PATIENTS AND METHODS:Forty-two patients, 17 with platinum-sensitive and 25 with platinum-resistant recurrent ovar...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2014.913617
更新日期:2014-06-01 00:00:00
abstract::Aberrant expression of apoptosis-related genes, including the "cell death suppressor gene" bcl-2, may play an important pathogenetic role in cancer and autoimmune diseases, In vivo upregulation of bcl-2 mRNA in synovial lining cells of patients with rheumatoid arthritis but not in patients with osteoarthritis has been...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979809034809
更新日期:1998-02-01 00:00:00
abstract:INTRODUCTION:Parsley (Petroselinum crispum) has been traditionally used for the treatment of allergy, autoimmune and chronic inflammatory disorders. The present study aims to investigate the suppressive effects of parsley essential oil on mouse splenocytes and macrophages cells. METHODS AND MATERIALS:Parsley essential...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2011.603338
更新日期:2012-04-01 00:00:00
abstract:OBJECTIVES:Fingolimod (FIN) is used for multiple sclerosis treatment and has potential antiapoptotic and anti-inflammatory effects. We aimed at expanding our knowledge on various immunohistochemical markers for elucidating the possible mechanisms of action of fingolimod in the placenta and fetal lung and brain. METHOD...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923973.2020.1818771
更新日期:2020-12-01 00:00:00
abstract::Contribution of cytotoxic T lymphocytes (CTL) to experimental autoimmune thyroiditis (EAT) was well defined (Speidel et al., Eur. J. Immunol. 1997, 27, 2391-2399, Ref. 7). The native porcine thyroglobulin (pTg) showed high sensitivity to endo-o-N-acetylglucosaminidase F (Endo F) and its molecular weights, correspondin...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章,收录出版
doi:10.1081/iph-200026872
更新日期:2004-08-01 00:00:00
abstract::N-nitrozodimethyloamine (NDMA) is a carcinogenic compound that can be formed in vivo. NDMA is synthesized from precursors-amines and nitrosating agents. Nitrosating agents are formed through the reaction of oxide, reactive oxygen species and nitric oxide (NO). Human neutrophils (PMN) are an important source of the mos...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970600625892
更新日期:2006-01-01 00:00:00
abstract::Phenolic composition and antioxidant activities of the aqueous extract of Arisaema cum Bile, which is widely used as a folk medicine in Korea, were determined. Phenolic composition profile revealed that the aqueous extract is rich in sinapic acid (13.14 mg/100 g extract), catechin (9.88 mg/100 g extract), neohesperidi...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2011.649289
更新日期:2012-08-01 00:00:00
abstract::During the last few years, several observations outline that the impaired T lymphocyte proliferative capacity in the elderly is due to a reduced interleukin 2 (IL-2) release. To further investigate the activation process during lectin stimulation, aged peripheral blood mononuclear cells (PBMC) were stimulated with phy...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979109019693
更新日期:1991-01-01 00:00:00
abstract:UNLABELLED:The angiotensin (Ang)-converting enzyme (ACE) insertion/deletion (I/D) polymorphism determines Ang II levels, but its relationship with lupus nephritis (LN) in different populations is controversial. OBJECTIVE:To describe the allelic and genotypic distribution of the I/D polymorphism in Mexican mestizos wit...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2012.739175
更新日期:2013-02-01 00:00:00
abstract::The purpose of our study was to compare the effectiveness of immunosuppressive drugs on the prevention of allograft rejection in a murine model of low-risk and high-risk keratoplasty. The therapy included FK 506 (tacrolimus; 0.2 mg/kg), mycophenolate mofetil (30 mg/kg), aminoguanidine (0.1 g/kg), and combination of FK...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970600809447
更新日期:2006-01-01 00:00:00