Abstract:
:Luminol-enhanced chemiluminescence assay was used to detect the surface expression and the consequent activation of receptors (FcRI and FcRII) of murine macrophages (M phi s). When murine IgG2a was used for the specific detection of FcRI and IgG2b for FcRII, a newly established procedure enabled us to detect the activation of each receptor with as few as 3 X 10(5) M phi s. Briefly, TNP-SRBC coated with monoclonal IgG2a or IgG2b antibodies directed to TNP (sensitized SRBC) were used as reagent, in the presence of 1 X 10(-5) M luminol, and the emission was measured with a liquid scintillation counter. When results obtained by chemiluminescence counting were compared to the results obtained by the rosette formation by adding the same SRBC reagent to peritoneal M phi s obtained after ip injection of Listeria, fortified chemiluminescence counting allowed us to obtain a more definite answer about the activation of each receptor. Under the conditions established, the specific activation of FcRI was obtained by the addition of rIFN alpha A/D to the resident M phi s in vitro and the specific activation of spleen M phi FcRII by iv injection of IAP (Immunosuppressive acidic protein) into mice. These two results supported the independence of the two receptors detected by the assay.
journal_name
Immunopharmacol Immunotoxicoljournal_title
Immunopharmacology and immunotoxicologyauthors
Majima T,Itoh K,Yatsu J,Yoshie O,Ishida Ndoi
10.3109/08923978909005371subject
Has Abstractpub_date
1989-01-01 00:00:00pages
289-307issue
2-3eissn
0892-3973issn
1532-2513journal_volume
11pub_type
杂志文章abstract::Objective: Acne is a chronic skin disease that involves four key pathogenic factors: excess sebum production, ductal epidermal hyperproliferation, Propionibacterium acnes (P. acnes) colonization, and skin inflammation. Mangostins are well-known for their anti-bacterial and anti-inflammatory effects, suggesting that ma...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923973.2018.1519831
更新日期:2018-10-01 00:00:00
abstract::The purpose of our study was to compare the effectiveness of immunosuppressive drugs on the prevention of allograft rejection in a murine model of low-risk and high-risk keratoplasty. The therapy included FK 506 (tacrolimus; 0.2 mg/kg), mycophenolate mofetil (30 mg/kg), aminoguanidine (0.1 g/kg), and combination of FK...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970600809447
更新日期:2006-01-01 00:00:00
abstract::The etiology of inflammatory bowel diseases which include ulcerative colitis (UC) and Crohn disease has not yet been clarified. Several hypotheses suggest a change in composition of gut microflora along with an impaired mucosal barrier that lead to excessive mucosal immunologic responses. Increased production of nitri...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2013.790413
更新日期:2013-06-01 00:00:00
abstract::The current research was designed to determine the effect of artemether in treatment of experimental rheumatoid arthritis. Collagen-induced arthritis was induced in Lewis rats. The intramusculary administration of artemether (ART) and intraperitoneally injection of methotrexate (MTX) were started on day 25 postimmuniz...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970500418786
更新日期:2005-01-01 00:00:00
abstract::We investigated the effect of Z-100, a polysaccharide-rich extract from Mycobacterium tuberculosis strain Aoyama B, on the protection of immunocompromised mice against infection with Pseudomonas aeruginosa. Mice were given cyclophosphamide and then treated with varying doses of Z-100 for 5 days or left untreated. One ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979009006467
更新日期:1990-01-01 00:00:00
abstract::Serum tumor necrosis factor (TNF) levels in 33 patients with inflammatory bowel disease (IBD) were measured by using a sensitive enzyme immunoassay. Four of five Crohn's diseases (CD) and nine of twenty eight ulcerative colitis (UC) had elevated levels of serum TNF. In active CD or UC, a greater fraction of patients s...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979209005404
更新日期:1992-01-01 00:00:00
abstract::Several abnormalities of the immune system have been reported in association with clinical and experimental iron overload. To dissect further such abnormalities, changes in lymphocyte subsets were evaluated in iron-loaded male Sprague-Dawley rats. The iron-loading protocol consisted of a total dose of irondextran (1.5...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979709038534
更新日期:1997-02-01 00:00:00
abstract::Myricetin, a common dietary flavonoid, is widely distributed in fruits and vegetables. It is known to be a food supplement contributing to human health because of its immune modulatory function, and its antioxidation, antitumor, and anti-inflammatory properties. In the present study, myricetin was shown to directly in...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2015.1096942
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Capsaicin is a chili pepper extract with multiple therapeutic properties including anti-liver fibrosis. However, the paucity of its underlying mechanisms limited its widely clinical application. METHODS:In the present study, carbon tetrachloride (CCl4) was used to induce liver fibrosis in mice, and transfor...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923973.2020.1811308
更新日期:2020-12-01 00:00:00
abstract::Plant medications have been used as treatment in various kinds of systemic inflammatory disorder such as Behçet's disease (BD). We investigated the roles of On-Chung-Eum (OCE), a traditional plant medicine, in cytokine regulation of BD. The effects of OCE on cytokine production from phytohaemagglutinin (PHA)-stimulate...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120018284
更新日期:2003-02-01 00:00:00
abstract::During the last few years, several observations outline that the impaired T lymphocyte proliferative capacity in the elderly is due to a reduced interleukin 2 (IL-2) release. To further investigate the activation process during lectin stimulation, aged peripheral blood mononuclear cells (PBMC) were stimulated with phy...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979109019693
更新日期:1991-01-01 00:00:00
abstract:AIM:To determine the steady state concentration and the pharmacokinetics of Cyclosporine A (CsA) in heart transplant recipients. METHODS:A single dose of 100 mg CsA capsules twice daily was given to five heart transplant recipients of steady state. The concentrations of CsA in plasma were determined by streptavidin pe...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 临床试验,杂志文章
doi:10.3109/08923973.2011.613400
更新日期:2012-06-01 00:00:00
abstract::In 15 elderly individuals lyophilized Bifidobacterium bifidum (BB) and Lactobacillus acidophilus (LA) (Infloran) were administered in capsules (two capsules 4 times per day) for 28 days, while in 10 elderly controls placebo were given the same posology and for an equal period of time. The effects of this treatment on ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 临床试验,杂志文章
doi:10.3109/08923979209009228
更新日期:1992-01-01 00:00:00
abstract::The recruitment of eosinophils from the circulation into the airway is a prominent feature of allergic asthma. Persistent inflammatory responses may arise from inefficient mechanisms for resolution of inflammation, including delayed apoptosis. Several studies suggest that eosinophil apoptosis is delayed in asthma. Sia...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章,评审
doi:10.3109/08923973.2011.589453
更新日期:2012-10-01 00:00:00
abstract::A human THP-1 monocyte cell line culture system has been utilized to observe the effect of granulocyte macrophage colony stimulating factor (GM-CSF) supplementation with lipopolysaccharide (LPS) of oral microorganisms to stimulate monocyte/macrophage functional activity. LPS of oral microorganisms, Fusobacterium nucle...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979809031512
更新日期:1998-11-01 00:00:00
abstract:UNLABELLED:The angiotensin (Ang)-converting enzyme (ACE) insertion/deletion (I/D) polymorphism determines Ang II levels, but its relationship with lupus nephritis (LN) in different populations is controversial. OBJECTIVE:To describe the allelic and genotypic distribution of the I/D polymorphism in Mexican mestizos wit...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2012.739175
更新日期:2013-02-01 00:00:00
abstract::The effect of rapamycin (RAPA) on kidney function and histopathology was assessed in two strains of mice. Male C3H/HeJ mice were treated with RAPA for 4 days at doses of 25, 50, 75, and 100 mg/kg, ip and male C3H/HeJ or female Balb/cJ mice were both treated for 7 days at doses of 75, 150 and 200 mg/kg. Cyclosporine (C...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979309066932
更新日期:1993-01-01 00:00:00
abstract::The protective effects of baicalin (BA), a major flavone from Scutellaria radix, on acetaminophen (AP)-induced hepatotoxicity and the possible mechanism(s) of its protective action were investigated in mice. Treatment with BA (300 mg/kg, p.o.) 0.5 h after AP administration significantly prevented an increase in plasma...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120026443
更新日期:2003-11-01 00:00:00
abstract::Modulation of immune response to alleviate diseases has long since been of interest. Plant extracts have been widely investigated for their possible immunomodulatory properties. We have evaluated the immunomodulatory activity of aqueous extract of Rhodiola rhizome in human peripheral blood mononuclear cells (PBMCs) an...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970600815139
更新日期:2006-01-01 00:00:00
abstract::The anti-cancer activity of saponins and phenolic compounds present in green tea was previously reported. However, the immunomodulatory and adjuvanticity activity of tea saponin has never been studied. In this study, we investigated the immunomodulatory effect of tea saponin in T-lymphocytes and EL4 cells via regulati...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2014.909849
更新日期:2014-06-01 00:00:00
abstract::A competitive enzyme-linked immunosorbent assay (ELISA) with generated mouse anti-consensus interferon (CIFN) antibody was developed for quantitative determination of pegylated consensus interferon (PEG-CIFN) in rhesus monkey serum. The operational concentrations of the original assay were determined using the chessbo...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923970902814111
更新日期:2009-01-01 00:00:00
abstract::Contribution of cytotoxic T lymphocytes (CTL) to experimental autoimmune thyroiditis (EAT) was well defined (Speidel et al., Eur. J. Immunol. 1997, 27, 2391-2399, Ref. 7). The native porcine thyroglobulin (pTg) showed high sensitivity to endo-o-N-acetylglucosaminidase F (Endo F) and its molecular weights, correspondin...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章,收录出版
doi:10.1081/iph-200026872
更新日期:2004-08-01 00:00:00
abstract::We have developed an in vitro system to model the interactions of drugs used to treat transplant rejection. This system consists of stimulation of human lymphocytes with a primary mitogen (anti-T-cell receptor complex antibodies (OKT3 or wt31)) and treatment with a primary immunosuppressive drug (ISD) (Cyclosporine A ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979409007089
更新日期:1994-05-01 00:00:00
abstract::Phenolic composition and antioxidant activities of the aqueous extract of Arisaema cum Bile, which is widely used as a folk medicine in Korea, were determined. Phenolic composition profile revealed that the aqueous extract is rich in sinapic acid (13.14 mg/100 g extract), catechin (9.88 mg/100 g extract), neohesperidi...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2011.649289
更新日期:2012-08-01 00:00:00
abstract::The immunological effects of interferon (IFN)-alpha administration were evaluated in 15 patients with cHCV infection. Individuals were treated with 6 MU of lymphoblastoid IFN-alpha three times a week for 6 months and with 3 MU three times a week for an additional 6 months. Patients were divided into responders (12 sub...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979609052741
更新日期:1996-08-01 00:00:00
abstract::We conducted a comparative study of NK1.1+ cells in spleen and bone marrow and the effects of administration of M-CSF on them. Administration of M-CSF to mice increased the number of NK1.1+ cells in spleen but not in bone marrow. The NK1.1+ cells in spleen (Spl-NK1.1) and bone marrow (BM-NK1.1) were purified by magnet...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120016035
更新日期:2002-11-01 00:00:00
abstract:AIM:To assess the immunogenicity of Peginterferon alpha-2 b(Pegberon) and its effect on the efficacy and safety in phase III clinical trial, by comparing it with the control drug Pegasys. METHODS:770 patients were recruited in total. 509 were treated with Pegberon plus ribavirin and 261 were treated with Pegasys plus ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1080/08923973.2017.1392565
更新日期:2018-02-01 00:00:00
abstract:BACKGROUND AND AIM:In the rat prostate, beta-adrenoreceptor (beta-AR) stimulation does not alter basal prostatic tone but may inhibit alpha1-AR-mediated, field stimulation-induced or receptor-independent contractile responses. The present study was designed to assess the alteration of basic fibroblast growth factor (bF...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970903164326
更新日期:2010-03-01 00:00:00
abstract::Arecoline, a major alkaloid of arecanut was screened to explore its modulatory influence on cell-mediated immune response in a murine model system. The in vivo and in vitro effects were evaluated at subtoxic concentrations of arecoline. Delayed type hypersensitivity (DTH) reactions to sheep red blood cells (SRBC) were...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979109019706
更新日期:1991-01-01 00:00:00
abstract::Superoxide and its derived active oxygen species are responsible for the polymorphonuclear leukocyte (PMN)-mediated tumoricidal activity which is typically shown in the intraperitoneal administration of OK-432, a biological response modifier, for cancer ascites. We examined the effects of intraperitoneal administratio...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979509019750
更新日期:1995-05-01 00:00:00