Functional changes in THP-1 human monocytic cells after stimulation with lipopolysaccharide of oral microorganisms and granulocyte macrophage colony stimulating factor.

Abstract:

:A human THP-1 monocyte cell line culture system has been utilized to observe the effect of granulocyte macrophage colony stimulating factor (GM-CSF) supplementation with lipopolysaccharide (LPS) of oral microorganisms to stimulate monocyte/macrophage functional activity. LPS of oral microorganisms, Fusobacterium nucleatum and Porphyromonas gingivalis was produced by phenol-water extraction and characterized. The phagocytosis assay was performed using F1TC labeled Saccharomyces yeast particles. Phagocytic functional activity was observed in 10-11% of resting THP-1 cells. Treatment of THP-1 cells with LPS of F. nucleatum or P. gingivalis increased the phagocytic activity of THP-1 cells 2-3 fold. GM-CSF significantly increased phagocytosis either alone or when supplemented with LPS of F. nucleatum or P. gingivalis. A chemotaxis assay was performed using a 48 well chemotaxis chamber. Chemotactic functional activity of THP-1 cells was increased 2-fold after 4 days of treatment with GM-CSF. Stimulation of THP-1 cells with LPS of F. nucleatum or P. gingivalis significantly reduced the chemotactic activity indicating the maturation towards a fixed macrophage. There were functional variations (chemotaxis and phagocytosis) in THP-1 cells in response to LPS of oral microorganisms following stimulation with GM-CSF.

authors

Baqui AA,Meiller TF,Turng BF,Kelley JI,Falkler WA Jr

doi

10.3109/08923979809031512

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

493-518

issue

4

eissn

0892-3973

issn

1532-2513

journal_volume

20

pub_type

杂志文章
  • Effect of short-term cocaine administration on the immune system of young and old C57BL/6 female mice.

    abstract::It has been shown that either cocaine or aging alone can alter the immune system. Our objective was to study if the immune system of aging mice was more susceptible to the effect of cocaine than the immune system of young mice. We used a short term (20 days) cocaine daily administration protocol. Cocaine only decrease...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923979909007140

    authors: Colombo LL,López MC,Chen GJ,Watson RR

    更新日期:1999-11-01 00:00:00

  • Chinese herbal medicinal ingredients affect secretion of NO, IL-10, ICAM-1 and IL-2 by endothelial cells.

    abstract::The aim of this study was to investigate the anti-endotoxin effects of sinomenine, fangchinoline, stachydrine, chuanxionggzine, oxymartrine and evodiamine alkaloids commonly found in Chinese herbal medicines. Porcine endothelial cells were challenged with 1 μg LPS/ml for 3 h and then treated with one of the six alkalo...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923973.2015.1046991

    authors: Hu Y,He K,Zhu H

    更新日期:2015-06-01 00:00:00

  • Atractylenolide I inhibits lipopolysaccharide-induced inflammatory responses via mitogen-activated protein kinase pathways in RAW264.7 cells.

    abstract::Atractylenolide I (ATL-I) is a bioactive component of Rhizoma Atractylodis macrocephalae. Although increasing evidence shows that ATL-I has an anti-inflammatory effect, the anti-inflammatory molecular mechanism of ATL-I is still unknown. In this study, we investigated the effect of ATL-I on cell viability by 3-(4, 5-D...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923973.2014.968256

    authors: Ji G,Chen R,Zheng J

    更新日期:2014-12-01 00:00:00

  • Oral administration of probiotic bacteria, Lactobacillus casei and Bifidobacterium breve, does not exacerbate neurological symptoms in experimental autoimmune encephalomyelitis.

    abstract::To evaluate the safety of two probiotic bacterial strains, Lactobacillus casei strain Shirota (LcS) and Bifidobacterium breve strain Yakult (BbY), these probiotics were orally administered to Lewis rats with experimental autoimmune encephalomyelitis (EAE), the experimental model of human multiple sclerosis. We examine...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923970903200716

    authors: Kobayashi T,Kato I,Nanno M,Shida K,Shibuya K,Matsuoka Y,Onoue M

    更新日期:2010-03-01 00:00:00

  • Lupus nephritis improvement after anti-tumor necrosis factor alpha monoclonal antibody (infliximab) treatment for Crohn's disease: a case report.

    abstract::Association between Crohn's disease (CD) and lupus nephritis is very rare and, to the best of our knowledge, it has been described only once. We report here a clinical case of CD occurred in a young woman 8 years after a diagnosis of lupus nephritis according to clinical, laboratory and histological criteria. CD was u...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.1081/iph-120037721

    authors: Principi M,Di Leo A,Ingrosso M,Pisani A,Marangi S,Amoruso A,Panella C,Francavilla A,Ierardi E

    更新日期:2004-05-01 00:00:00

  • Anti-angiogenic activity of Ipomoea obscura extract and Ipobscurine-A.

    abstract:OBJECTIVE:Ipomoea obscura (L.) Ker-Gawl, is a medicinal herb with indole alkaloids as an active constituent. In this study, we investigated the anti-angiogenic activity of I. obscura extract and one of its major compounds Ipobscurine-A (IPO-A). METHODS:In vivo angiogenesis was induced by injecting B16F10 melanoma cell...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923973.2010.531277

    authors: Hamsa TP,Kuttan G

    更新日期:2011-09-01 00:00:00

  • Immunomodulatory and antitumor activity of Aerva lanata ethanolic extract.

    abstract::Cancer is responsible for millions of deaths each year worldwide. Pharmacological intervention with plant-derived products alone or in combination to reverse, suppress, or prevent the cancer progression plays a key role in the fight against this terrible disease. Aerva lanata is an important medicinal plant widely use...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923973.2010.526614

    authors: Siveen KS,Kuttan G

    更新日期:2011-09-01 00:00:00

  • IgA directly inhibits antigen-dependent B cell activation following distinctive distribution of the antigen in mice.

    abstract:CONTEXT:Serum IgA suppresses immune responses when exposed to antigens recognized by the antibody; however, the underlying mechanism remains unclear. OBJECTIVE:We herein clarified the relationships between changes in antigen distribution and antigen-dependent B cell activation in the presence or absence of IgA against...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923973.2016.1142559

    authors: Yamaki K,Yoshino S

    更新日期:2016-01-01 00:00:00

  • Mechanisms of diabetes mellitus induced with FK506 in SD rats models.

    abstract:BACKGROUND:Tacrolimus causes post-transplant diabetes mellitus, however the pathogenetic mechanisms remain controversial. In this study we probed into the mechanisms of tacrolimus-induced diabetes mellitus in rats. METHODS:Glucose levels were determined on whole blood samples using a glucose oxidase method. Levels of ...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923970903032747

    authors: Li QY,Li F,Sun JH,Chi YY,Yin HS,Liu SQ,Mei YH

    更新日期:2009-01-01 00:00:00

  • Immunomodulatory effects of the pentapeptide YGSRS on human peripheral-blood mononuclear cells.

    abstract:CONTEXT:The pentapeptide YGSRS is originated from coffee bean, while its pharmacological features have little been examined. OBJECTIVES:We investigated the effects of YGSRS on proliferation, cytokine production and CD4+ CD25+ Foxp3+ regulatory T (Treg) cell frequency of human peripheral blood mononuclear cells (PBMCs)...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 临床试验,杂志文章

    doi:10.3109/08923973.2015.1038752

    authors: Nakamura Y,Hayakawa A,Ishizawa H,Kamei Y,Tanaka S,Onda K,Sugiyama K,Hirano T

    更新日期:2015-06-01 00:00:00

  • Enhancement of lymphocyte proliferation and IL-2 receptor expression by a processed form (GM-1/P) of monosialoganglioside GM-1.

    abstract::In this study we investigated the ability of GM-1/P, a calcium mediated processed form of monosialoganglioside GM-1, of in vivo augmenting mouse T and B-lymphocyte blastogenesis induced by mitogens. We have also determined its effect on IL-2 responsiveness by analyzing the induction of the expression of IL-2 receptor ...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923979009019677

    authors: Giuffrida A,Morrone S,Gismondi A,Santoni G,Punturieri A,Velotti F,Cavallo G,Tubaro E,Piccoli M,Frati L

    更新日期:1990-01-01 00:00:00

  • Modulating effect of Withania somnifera on TCA cycle enzymes and electron transport chain in azoxymethane-induced colon cancer in mice.

    abstract::The aim of the present investigation was to evaluate the efficacy of Withania somnifera on tricarboxylic acid (TCA) cycle enzymes and electron transport chain in azoxymethane-induced experimental colon cancer in mice. Azoxymethane at the dose of 15 mg/kg body weight was induced intraperitoneally once in a week for 28 ...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923970903581540

    authors: Muralikrishnan G,Amanullah S,Basha MI,Dinda AK,Shakeel F

    更新日期:2010-09-01 00:00:00

  • Neutrophil peripheral count and human leukocyte elastase during chronic lithium carbonate therapy.

    abstract::Plasma levels of human polymorphonuclear elastase (PMN-E) are considered a marker of granulocyte activation and can potentially complement the peripheral neutrophil count in laboratory and pathophysiological settings. Neutrophilic leukocytosis is a well known effect of lithium therapy, but there is no information abou...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923970009016431

    authors: Capodicasa E,Russano AM,Ciurnella E,De Bellis F,Rossi R,Scuteri A,Biondi R

    更新日期:2000-11-01 00:00:00

  • Apigenin inhibits release of inflammatory mediators by blocking the NF-κB activation pathways in the HMC-1 cells.

    abstract::Apigenin is a plant flavonoid and a pharmacologically active agent that has been isolated from several plant species. However, the molecular mechanism of apigenin-mediated immune modulation has not been fully understood. One of the possible mechanisms of its protective effects is the down-regulation of inflammatory re...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923973.2010.538851

    authors: Kang OH,Lee JH,Kwon DY

    更新日期:2011-09-01 00:00:00

  • Granulocyte macrophage colony-stimulating factor is required for cytokine induction by a highly 6-branched 1,3-β-D-glucan from Aureobasidium pullulans in mouse-derived splenocytes.

    abstract::We have previously obtained and elucidated the precise structure of a highly branched 1,3-β-D-glucan (with 6-monoglucopyranosyl side chains), Aureobasidium pullulans-fermented β-D-glucan (AP-FBG), from the fungus A. pullulans. However, the mechanism(s) of the effects of AP-FBG on in vitro mouse primary cells have not ...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923973.2010.503707

    authors: Tada R,Yoshikawa M,Kuge T,Tanioka A,Ishibashi K,Adachi Y,Tsubaki K,Ohno N

    更新日期:2011-06-01 00:00:00

  • Continuous veno-venous hemodiafiltration alleviates multiple organ dysfunction syndrome in dogs.

    abstract:CONTEXT:Multiple organ dysfunction syndrome (MODS) is a major cause of death in critically patients. It has been hypothesized that inactivation or removal of pro-inflammatory molecules may prevent or reverse MODS. OBJECTIVE:The purpose of this paper was to investigate the efficacy of continuous veno-venous hemodiafilt...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923973.2012.685630

    authors: Chen J,Liu J,Yu C,Wen H

    更新日期:2012-12-01 00:00:00

  • Growth-inhibitory effects of a Bulnesia sarmienti aqueous extract on A549 cells in vitro and S180 cells in vivo.

    abstract::The effects of Bulnesia sarmienti (BS) aqueous extract on the cell growth of A549 cell lines were investigated. BS has strong cytotoxic activity on the A549 cell lines (IC(50); less than 100 microg/mL) in MTT assay. HPLC confirmed that BS contains catechins as major compound. Cell cycle analysis by flow cytometry indi...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.1080/08923970902810432

    authors: Mollah ML,Kim JO,Lee GD,Park CH,Hong JH,Kim HY,Kim KS

    更新日期:2009-01-01 00:00:00

  • Differential effects of ponesimod, a selective S1P1 receptor modulator, on blood-circulating human T cell subpopulations.

    abstract::Ponesimod, a novel selective sphingosine-1-phosphate 1 receptor modulator in the development for the treatment of autoimmune diseases, dose-dependently reduced lymphocyte counts in peripheral blood of healthy subjects. It rapidly and transiently reduced the number of circulating T and B cells, but not natural killer c...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章,随机对照试验

    doi:10.3109/08923973.2014.993084

    authors: D'Ambrosio D,Steinmann J,Brossard P,Dingemanse J

    更新日期:2015-02-01 00:00:00

  • In-line affinity chromatographic removal of specific antibody from rabbits with experimental myasthenia gravis as a prelude to immunotherapy.

    abstract::Myasthenia gravis is an autoimmune disease in which antibodies are produced against one's acetylcholine receptors, resulting in complement-mediated membrane destruction and internalization of antibody-receptor complexes. Symptoms range from weakening of extraocular muscles to severe impairment of movement and breathin...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923979809038542

    authors: Hinman CL,Stevens-Truss R

    更新日期:1998-05-01 00:00:00

  • Anti-allergic effects of Sanguisorba officinalis on animal models of allergic reactions.

    abstract::We investigated the effect of aqueous extract of Sanguisorba officinalis L.(Rosaceae) root (SOAE) on the immediate-type allergic reactions in vivo and in vitro. SOAE (0.01 to 1 g/kg) inhibited systemic allergic reaction induced by compound 48/80. When SOAE was employed in a systemic allergic reaction test, the plasma ...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.1081/iph-120014729

    authors: Shin TY,Lee KB,Kim SH

    更新日期:2002-08-01 00:00:00

  • CD69 and regulation of the immune function.

    abstract::CD69, also known as activation inducer molecule, very early activation antigen, MLR-3 and Leu-23, is a member of the natural killer (NK) cell gene complex family of signal transducing receptors. CD69 is as a type II transmembrane glycoprotein with a C-type lectin binding domain in the extracellular portion of the mole...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章,评审

    doi:10.3109/08923979909007126

    authors: Marzio R,Mauël J,Betz-Corradin S

    更新日期:1999-08-01 00:00:00

  • Feasibility study of personalized peptide vaccination for recurrent ovarian cancer patients.

    abstract:CONTEXT:To develop a personalized peptide vaccine (PPV) for recurrent ovarian cancer patients and evaluate its efficacy from the point of view of overall survival (OS), Phase II study of PPV was performed. PATIENTS AND METHODS:Forty-two patients, 17 with platinum-sensitive and 25 with platinum-resistant recurrent ovar...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923973.2014.913617

    authors: Kawano K,Tsuda N,Matsueda S,Sasada T,Watanabe N,Ushijima K,Yamaguchi T,Yokomine M,Itoh K,Yamada A,Kamura T

    更新日期:2014-06-01 00:00:00

  • Immunohistochemical analysis of protective effects of maternal fingolimod on the placenta and fetal lung and brain in chorioamnionitis-induced preterm birth rat model.

    abstract:OBJECTIVES:Fingolimod (FIN) is used for multiple sclerosis treatment and has potential antiapoptotic and anti-inflammatory effects. We aimed at expanding our knowledge on various immunohistochemical markers for elucidating the possible mechanisms of action of fingolimod in the placenta and fetal lung and brain. METHOD...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.1080/08923973.2020.1818771

    authors: Yavuz A,Sezik M,Eris Yalcin S,Asci H,Ozmen O

    更新日期:2020-12-01 00:00:00

  • Immunomodulatory role of recombinant human erythropoietin in acute kidney injury induced by crush syndrome via inhibition of the TLR4/NF-κB signaling pathway in macrophages.

    abstract::Objective: The present study aimed to investigate whether recombinant human erythropoietin (rHuEPO) plays an immunomodulatory function by regulating the TLR4/NF-κB signaling pathway.Materials and methods: C57BL/6 mice were intraperitoneally injected with rHuEPO and, half an hour later, with 50% glycerol at the dose of...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.1080/08923973.2019.1706555

    authors: Zhou J,Bai Y,Jiang Y,Tarun P,Feng Y,Huang R,Fu P

    更新日期:2020-02-01 00:00:00

  • Influence of dietary aluminum on cytokine production by mitogen-stimulated spleen cells from Swiss Webster mice.

    abstract::Swiss Webster mice were exposed to excess dietary aluminum (Al) (1000 micrograms Al/g diet, Al as Al lactate) from conception to 6 months of age. Splenic lymphocytes (10(6) per culture) were incubated for 24 hrs with concanavalin A (5 micrograms/ml). Concentrations of interleukin-2, interferon-gamma and tumor necrosis...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923979309019733

    authors: Golub MS,Takeuchi PT,Gershwin ME,Yoshida SH

    更新日期:1993-11-01 00:00:00

  • Effect of anesthesia on a whole blood lymphocyte proliferation assay in the rat.

    abstract::Lymphocyte proliferation assays are commonly used to quantify the effects of immunosuppressive drugs in animal models, but the influence of anesthetic agents on those assays is not well understood. We used a whole blood proliferation assay to compare lymphocyte proliferation in blood drawn from normal male Lewis rats ...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923979909052762

    authors: Gummert JF,Otto G,Barten MJ,Morris RE

    更新日期:1999-05-01 00:00:00

  • The effects of cadmium exposure in the induction of inflammation.

    abstract::Inflammation is a physiological process essential for maintaining homeostatic mechanisms in human, but however, exaggerated inflammatory responses are closely related to many chronic diseases. Cadmium (Cd) is a heavy metal with high toxicity when present in food, water and air has the potential of eliciting inflammato...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章,评审

    doi:10.1080/08923973.2019.1697284

    authors: Hossein-Khannazer N,Azizi G,Eslami S,Alhassan Mohammed H,Fayyaz F,Hosseinzadeh R,Usman AB,Kamali AN,Mohammadi H,Jadidi-Niaragh F,Dehghanifard E,Noorisepehr M

    更新日期:2020-02-01 00:00:00

  • Tanshinone IIA protects against immune-mediated liver injury through activation of T-cell subsets and regulation of cytokines.

    abstract:BACKGROUND AND AIM:Tanshione IIA (TSN) is the major active component of Salvia miltiorrhiza, a traditional Chinese Medicine. TSN protects against antioxidant-induced liver injury, although the exact mechanism is not well understood. MATERIALS AND METHODS:In this study, the protective effects of TSN was examined by enz...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.1080/08923970903120997

    authors: Qin XY,Li T,Yan L,Liu QS,Tian Y

    更新日期:2010-03-01 00:00:00

  • Insertion/deletion polymorphism of the angiotensin-converting enzyme gene in lupus nephritis among Mexicans.

    abstract:UNLABELLED:The angiotensin (Ang)-converting enzyme (ACE) insertion/deletion (I/D) polymorphism determines Ang II levels, but its relationship with lupus nephritis (LN) in different populations is controversial. OBJECTIVE:To describe the allelic and genotypic distribution of the I/D polymorphism in Mexican mestizos wit...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923973.2012.739175

    authors: Topete-Reyes JF,Soto-Vargas J,Morán-Moguel MC,Dávalos-Rodríguez IP,Chávez-González EL,García-de la Torre I,Parra-Michel R,Medina-Pérez M,Jalomo-Martínez B,Salazar-Páramo M

    更新日期:2013-02-01 00:00:00

  • Beta-adrenoceptor responsiveness of splenic macrophages in normotensive and hypertensive rats.

    abstract::The aim of the present study was to investigate putative mechanisms implicated in the impaired phagocytic response of spontaneously hypertensive rats (SHR)1. The effect of in vitro treatment with isoproterenol (ISO), a beta-adrenergic drug, on phagocytosis and respiratory burst by splenic macrophages (SpMø) from normo...

    journal_title:Immunopharmacology and immunotoxicology

    pub_type: 杂志文章

    doi:10.3109/08923979609052735

    authors: Serio M,Potenza MA,Montagnani M,Mansi G,Mitolo-Chieppa D,Jirillo E

    更新日期:1996-05-01 00:00:00