Protein kinase B is involved in Nogo-66 inhibiting neurite outgrowth in PC12 cells.

Abstract:

:Nogo-A, a member of the reticulon family, is one of the most important myelin-associated inhibitors for axonal growth, regeneration, and plasticity in the central nervous system. RhoA has been targeted pharmacologically to promote neurite outgrowth and functional recovery in the brain and spinal cord. However, the underlying mechanism of the inhibition of neurite outgrowth by Nogo-A has not yet been fully defined. Protein kinase B (PKB, also known as Akt) is a protein serine/threonine kinase that plays a key role in intracellular signaling and cellular homeostasis. This study reports the role of PKB signaling on Nogo-A-treated PC12 neuronal cells. An inhibitory fragment of Nogo-A (Nogo-66) activated RhoA and reduced the phosphorylation of PKB at Ser473 in a time-dependent manner. In contrast, pretreatment with Y27632, a specific inhibitor of Rho-A, resulted in an increase of the phosphorylation of PKB. Nogo-66 also inhibited the neurite outgrowth of PC12 cells, whereas pretreatment with LY294002, a specific inhibitor of PKB, ameliorated the neurite outgrowth. These data suggest that PKB is involved in the inhibition of neurite outgrowth by Nogo-A in PC12 cells.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Wang H,Shen J,Xiong N,Zhao H,Chen Y

doi

10.1097/WNR.0b013e32834a58e8

subject

Has Abstract

pub_date

2011-10-26 00:00:00

pages

733-8

issue

15

eissn

0959-4965

issn

1473-558X

journal_volume

22

pub_type

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