Neonatal thymulin gene therapy in nude mice: Effects on the morphology of the pituitary corticotrope population.

Abstract:

:The integrity of the thymus during early life is necessary for a proper maturation of the neuroendocrine system, including the adrenal axis. The thymic metallopeptide thymulin seems to be a central physiologic mediator of thymus-pituitary communication. Furthermore, neonatal thymulin gene therapy has been shown to prevent the typical alterations of gonadotrophic cell number and morphology and serum gonadotropin levels in nude female mice. In the present study we assessed the impact of athymia and the effect of neonatal thymulin gene therapy on the corticotropic cell population in nude mice. The effect of thymulin administration to adult nudes on their hypothalamic content of corticotropin-releasing hormone (CRH) and the adrenal content of corticosterone was also determined. We used an adenoviral vector expressing a synthetic gene for the thymic peptide thymulin (metFTS) termed RAd-FTS. On postnatal day 1 or 2, heterozygous (nu/+) and homozygous (nu/nu) pups of both sexes received a single bilateral i.m. injection of RAd-FTS or RAd-GFP, a control vector. On postnatal day 71, mice were bled and sacrificed, and their pituitaries were immediately dissected, fixed and immunostained for corticotropin. Morphometry was performed by means of an image-analysis system. The following parameters were calculated: volume density (VD: Σ cell area/reference area), cell density (CD: number of cells/reference area), and cell surface (CS: expressed in μm²). Serum thymulin levels were measured by a bioassay, and CRH as well as corticosterone were determined by IRMA and RIA, respectively. Neonatal thymulin gene therapy in the athymic mice restored their serum thymulin levels and increased corticotrope CD, VD and CS in both control and athymic mice. Athymic mice showed only a marginal reduction in corticotrope CD, VD and CS. In these mutants hypothalamic CRH content was slightly increased, whereas adrenal corticosterone tended to be lower. Thymulin administration to adult mice tended to reverse these changes. Our results suggest a possible modulating effect of thymulin on the corticotrope population and the adrenal gland, confirming the existence of a bidirectional thymus-pituitary-adrenal axis.

journal_name

Histol Histopathol

authors

Martines E,Reggiani PC,Schwerdt JI,Goya RG,Cónsole G

doi

10.14670/HH-26.471

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

471-9

issue

4

eissn

0213-3911

issn

1699-5848

journal_volume

26

pub_type

杂志文章
  • The relative effects of severe burn injury and pre- and post-natal protein deprivation on mandibular condyle morphology.

    abstract::The mandible has a mixed embryological origin, and its growth is associated with the secondary cartilage of the condyle process (CP). In this area, growth depends on an array of intrinsic and extrinsic factors that influence protein metabolism. In the present study, we used an adolescent rat model to evaluate the grow...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-25.45

    authors: de Oliveira BC,de Oliveira F,Martini DT,Prisco CR,da Silva Riguetti MM,Liberti EA,de Campos Boldrini S

    更新日期:2010-01-01 00:00:00

  • Vacuolar-type proton pump ATPases: roles of subunit isoforms in physiology and pathology.

    abstract::The vacuolar-type H(+)-ATPases (V-ATPases) are a family of multi-subunit ATP-dependent proton pumps involved in diverse cellular processes, including acid/base homeostasis, receptor-mediated endocytosis, processing of proteins and signaling molecules, targeting of lysosomal enzymes, and activation of various degradati...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-25.1611

    authors: Sun-Wada GH,Wada Y

    更新日期:2010-12-01 00:00:00

  • Overexpression of YES1 is associated with favorable prognosis and increased platinum-sensitivity in patients with epithelial ovarian cancer.

    abstract:AIMS:The prognostic application of YES1 in epithelial ovarian cancer (EOC) is currently unclear. We aimed to investigate the expression of YES1 and its correlation with survival outcome in patients with EOC. METHODS:A retrospective study of patients diagnosed with EOC at the Cancer Center, Sun Yat-Sen University, Guan...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-18-203

    authors: Zhou Y,Chen P,Huang Q,Wan T,Jiang Y,Jiang S,Yan S,Zheng M

    更新日期:2020-07-01 00:00:00

  • Histology and ultrastructure of the pineal organ in the domestic goose.

    abstract::The pineal organs of 14-week-old domestic geese were investigated with light and electron microscopy. The pineals consisted of a wide distal part and a narrow middle-proximal one. The glands were attached to the intercommissural region via the choroid plexus. The pineal parenchyma was formed by round or elongated foll...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-21.1075

    authors: Prusik M,Lewczuk B,Nowicki M,Przybylska-Gornowicz B

    更新日期:2006-10-01 00:00:00

  • Expression of cyclooxygenase-2 in invasive breast carcinomas and its prognostic impact.

    abstract::Representative tumour sections from 468 patients with invasive breast cancer were immunostained for cyclooxygenase-2 (COX-2) and evaluated. The relationships between COX-2 expression, clinical outcome and various clinicopathological variables, including tumour vascularity and disseminated tumour cells (DTC) in the bon...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-27.1315

    authors: Dhakal HP,Naume B,Synnestvedt M,Borgen E,Kaaresen R,Schlichting E,Wiedswang G,Bassarova A,Holm R,Giercksky KE,Nesland JM

    更新日期:2012-10-01 00:00:00

  • Fine structure of the retinal photoreceptors of the barred owl (Strix varia).

    abstract::The photoreceptors of the barred owl (Strix varia) consist of rods, single cones and unequal double cones present in a ratio of about 35:1:3. In the light-adapted condition the rods are of uniform diameter along their entire length and are therefore not felt to undergo photomechanical changes. The rod outer segment co...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Braekevelt CR,Smith SA,Smith BJ

    更新日期:1996-01-01 00:00:00

  • Comparison of the dysadherin and E-cadherin expression in primary lung cancer and metastatic sites.

    abstract::Dysadherin, a cancer associated cell membrane glycoprotein, has been reported to downregulate E-cadherin. Aberrant expression of E-cadherin has been associated with the development of metastases in patients with cancer. Even though the expression of dysadherin and E-cadherin has been studied in primary non-small cell ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-25.1257

    authors: Mitselou A,Batistatou A,Nakanishi Y,Hirohashi S,Vougiouklakis T,Charalabopoulos K

    更新日期:2010-10-01 00:00:00

  • Effects of pneumadin (PNM) on the adrenal glands. 6. Further studies on the inhibitory effect of PNM on dexamethasone-induced atrophy of the rat adrenal cortex.

    abstract::Pneumadin (PNM) is a biologically active decapeptide, which has previously been found to enhanced rat adrenal growth; the mechanism is indirect and probably involves the stimulation of both arginine-vasopressin (AVP) and ACTH release. The effects of 2- and 6-day PNM administration on the atrophic adrenal cortices of r...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Markowska A,Andreis PG,Miskowiak B,Nussdorfer GG,Malendowicz LK

    更新日期:1997-07-01 00:00:00

  • Analysis of wt1a reporter line expression levels during proepicardium formation in the zebrafish.

    abstract::The epicardium is the outer mesothelial layer of the heart. It covers the myocardium and plays important roles in both heart development and regeneration. It is derived from the proepicardium (PE), groups of cells that emerges at early developmental stages from the dorsal pericardial layer (DP) close to the atrio-vent...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-18-238

    authors: Andrés-Delgado L,Galardi-Castilla M,Mercader N,Santamaría L

    更新日期:2020-09-01 00:00:00

  • Retinal afferents on Golgi-identified vertical neurons in the superior colliculus of the rabbit. A Golgi-EM, degenerative and autoradiographic study.

    abstract::The characteristics and distribution pattern of retinal afferent terminals making synaptic contacts on narrow field vertical neurons in the stratum griseum superficiale of the rabbit superior colliculus were studied using the Golgi-gold substitution technique in combination with either autoradiographic or degenerative...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Ortega F,Doñate-Oliver F,Grandes P

    更新日期:1993-01-01 00:00:00

  • An ultrastructural stereologic study of aldosterone secreting adrenal adenomas and of adjacent zona glomerulosa.

    abstract::The ultrastructure of four aldosterone secreting adenomas and of the adjacent zona glomerulosa has been described by the use of stereological techniques. Adenomatous cells (about 2800 microns 3 in volume) invariably displayed a striking abundance of lipid droplets, which occupied about 30% of the cytoplasm. Mitochondr...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Belloni AS,Mantero F,Rocco S,Gottardo G,Mazzocchi G,Nussdorfer GG

    更新日期:1988-07-01 00:00:00

  • MgSO4 treatment preserves the ischemia-induced reduction in S-100 protein without modification of the expression of endothelial tight junction molecules.

    abstract:UNLABELLED:The aim of this work was to evaluate the effect of magnesium sulphate (MgSO4) administration on blood-brain barrier (BBB) permeabilization after cerebral hypoxia-ischemia (HI) induced by partial occlusion of the umbilical cord of premature fetal lambs. We also characterized BBB dysfunction in terms of the le...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-24.1129

    authors: Goñi-de-Cerio F,Alvarez A,Alvarez FJ,Rey-Santano MC,Alonso-Alconada D,Mielgo VE,Gastiasoro E,Hilario E

    更新日期:2009-09-01 00:00:00

  • Pathological changes of human unmyelinated nerve fibers: a review.

    abstract::In the cutaneous nerves, unmyelinated nerve fibers outnumber the myelinated ones but are scarcely analyzed, especially at autopsy. This indifference toward the pathology of unmyelinated nerve fibers may be due to the necessity of electron microscopic analyses and, more importantly, the obscurity of pathological altera...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-15.313

    authors: Kanda T

    更新日期:2000-01-01 00:00:00

  • Histochemical aspects of the yolk-sac and digestive tract of larvae of the Senegal sole, Solea senegalensis (Kaup, 1858).

    abstract::Histochemical distribution of glycoproteins, carbohydrates and proteins rich in different amino acids were studied using histological and histochemical procedures, in Senegal sole, Solea senegalensis (Kaup, 1858) larvae from hatching until day 15. Glycogen, proteins and glycoproteins were detected in the yolk-sac of t...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Sarasquete C,González de Canales ML,Arellano JM,Muñoz-Cueto JA,Ribeiro L,Dinis MT

    更新日期:1996-10-01 00:00:00

  • Unexpected presence of the neurotrophins NGF and BDNF and the neurotrophin receptor p75 in the tendon cells of the human Achilles tendon.

    abstract::Neurotrophins are substances that have been shown to be important in growth and remodelling phases in different types of tissue. There is no information concerning the possible occurrences of neurotrophins and their receptors in tendons. In this study, sections of both chronic painful (tendinosis) and pain-free (non-t...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-24.839

    authors: Bagge J,Lorentzon R,Alfredson H,Forsgren S

    更新日期:2009-07-01 00:00:00

  • Apocrine secretory mechanism: recent findings and unresolved problems.

    abstract::Cell secretion is an important physiological process that ensures smooth metabolic activities, tissue repair and growth and immunological functions in the body. It occurs when the intracellular secretory materials are released to the exterior; these may be in the form of lipids, protein or mucous and may travel throug...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-18.597

    authors: Gesase AP,Satoh Y

    更新日期:2003-04-01 00:00:00

  • Age-related quantitative changes in the organelles of rat neocerebellar Purkinje cells.

    abstract::A quantitative study regarding the age-related changes occurring in the somatic organelles of the neocerebellar Purkinje cell is carried out, using female rats aged 2 to 24 months. Standard manual morphometric techniques are used to calculate the following parameters: somatic volume, volumetric fractions and absolute ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Monteiro RA

    更新日期:1991-01-01 00:00:00

  • Differential severity of LPS-induced lung injury in CD26/DPP4 positive and deficient F344 rats.

    abstract:BACKGROUND:Lipopolysaccharide (LPS) induced inflammation often leads to lung injury, in which pulmonary recruitment of neutrophils plays a pivotal role. Inflammatory processes are influenced by CD26/DPP4, highly expressed in lungs. Asthma induced CD26/DPP4 deficient (CD26/DPP4⁻) Fischer (F) 344 rats suffering from a tr...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-18-117

    authors: Zientara A,Stephan M,von Hörsten S,Schmiedl A

    更新日期:2019-10-01 00:00:00

  • Morphology, differentiation and matrix production of liver cells in organoid cultures (high density cultures) of fetal rat livers.

    abstract::The aim of this study was to demonstrate the morphology and matrix synthesis of embryonic rat liver cells (day 18 of gestation) in organoid cultures (high density cultures) with electron microscopic and immunomorphological techniques. For this purpose the cells of embryonic rat livers were isolated enzymatically and g...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Elkasaby A,Xu D,Schröter-Kermani C,Merker HJ

    更新日期:1991-04-01 00:00:00

  • Domestic microwave processing for rapid immunohistochemical diagnosis of bovine rabies.

    abstract::The present study describes the use of a microwave processing protocol for the rapid histopathological and immunohistochemical diagnosis of bovine rabies. Immunohistochemistry has been used for rabies diagnosis in formalin-fixed tissue with satisfactory results, although the time to diagnosis is considerably longer th...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-27.1227

    authors: Abreu CC,Nakayama PA,Nogueira CI,Mesquita LP,Lopes PF,Varaschin MS,Seixas Jdo N,Ferreira E,Bezerra PS Jr

    更新日期:2012-09-01 00:00:00

  • An up-date on newly discovered immunohistochemical biomarkers for the diagnosis of human testicular germ cell tumors.

    abstract::Although testicular germ cell tumors (TGCTs) are relatively uncommon, they are particularly important, as they tend to affect children and young men, the most common tumor being in males aged from 20 to 40 years, and the incidence has been increasing in the last years. TGCTs comprise two major histologic groups: semin...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-29.999

    authors: Chieffi P,Chieffi S

    更新日期:2014-08-01 00:00:00

  • Structure and function of melanocytes: microscopic morphology and cell biology of mouse melanocytes in the epidermis and hair follicle.

    abstract::Melanocytes characterized by their tyrosinase activity, melanosomes and dendrites locate in the basal layer of epidermis and hair bulb in the skin of mice. Melanocytes differentiate from undifferentiated melanoblasts derived from embryonic neural crest. Melanocyte-stimulating hormone plays an important role in the reg...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:

    authors: Hirobe T

    更新日期:1995-01-01 00:00:00

  • Control of the cell cycle by neurotrophins: lessons from the p75 neurotrophin receptor.

    abstract::Although traditionally little attention has been paid to the interplay between neurotrophins and the cell cycle, a number of recent findings suggest an important role for these growth factors in the regulation of this aspect of the cellular physiology. In this article, we review the evidence from a number of studies t...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-17.1227

    authors: López-Sánchez N,Frade JM

    更新日期:2002-10-01 00:00:00

  • Expression of mitotic kinases phospho-aurora A and aurora B correlates with clinical and pathological parameters in bladder neoplasms.

    abstract::Aurora A and Aurora B are serine-threonine kinase proteins which have both been implicated in human carcinogenesis through development of aneuploidy and chromosomal instability. The aim of the study is to assess the correlation of both markers with clinical and pathological parameters in patients with bladder cancer o...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-25.1371

    authors: Bufo P,Sanguedolce F,Tortorella S,Cormio L,Carrieri G,Pannone G

    更新日期:2010-11-01 00:00:00

  • Elastin variations implicating in vascular smooth muscle cells phenotype in human tortuous arteries.

    abstract::The aim of the present work was to study the morphological implications between the elastin and the phenotypic expression of the vascular smooth muscle cells. For this purpose, sixty human tortuous arteries from different territories have been studied. We have measured the morphometric indexes Intimal Thickening Index...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-15.95

    authors: Ortiz PP,Sarrat R,Daret D,Whyte J,Torres A,Lamazière JM

    更新日期:2000-01-01 00:00:00

  • Liver growth factor antifibrotic activity in vivo is associated with a decrease in activation of hepatic stellate cells.

    abstract::The antifibrotic activity of Liver Growth Factor (LGF), a liver mitogen, was previously demonstrated in several models of rat liver fibrosis and even in extrahepatic sites, such as carotid artery in hypertensive rats and rat CdCl2-induced lung fibrosis. In the present study, we have attempted to examine in depth its m...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:10.14670/HH-24.473

    authors: Díaz-Gil JJ,García-Monzón C,Rúa C,Martín-Sanz P,Cereceda RM,Miquilena-Colina ME,Machín C,Fernández-Martínez A,García-Cañero R

    更新日期:2009-04-01 00:00:00

  • Toxicity of non-steroidal anti-inflammatory drugs: a review of melatonin and diclofenac sodium association.

    abstract::Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used for the purpose of anti-inflammation, antipyretic, and analgesia. For this aim, they are used for the alleviation of pain, fever, and inflammation associated with rheumatoid arthritis, sports injuries, and temporary pain. However, treatment with NSAIDs may ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-27.417

    authors: Aygün D,Kaplan S,Odaci E,Onger ME,Altunkaynak ME

    更新日期:2012-04-01 00:00:00

  • Paracrine role for TGF-β-induced CTGF and VEGF in mesangial matrix expansion in progressive glomerular disease.

    abstract::Transforming growth factor-β (TGF-β) is a key regulator of extracellular matrix (ECM), and may mediate the development of glomerulosclerosis with accumulation of mesangial matrix. Mesangial cells secrete TGF-β in response to common in vitro fibrogenic stimuli. Yet mesangial immunostaining for active TGF-β1 is frequent...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审

    doi:10.14670/HH-27.1131

    authors: Lee HS

    更新日期:2012-09-01 00:00:00

  • Ultrastructural studies on myofibrillogenesis and neogenesis of skeletal muscles after prolonged traction in rabbits.

    abstract::Little is known about the morphological response of muscle after long term traction. The purpose of this study was to investigate the morphological changes of skeletal muscle during limb lengthening. After application of mini-extraskeletal fixator, the hindlimb of New Zealand white rabbit was osteotomized and then slo...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Sun JS,Hou SM,Hang YS,Liu TK,Lu KS

    更新日期:1996-04-01 00:00:00

  • Localization of serotonin, cholecystokinin and somatostatin immunoreactivity in the lower respiratory tract of embryonic, foetal and postnatal sheep.

    abstract::The lower respiratory tract of the sheep was studied by light-microscopical immunocytochemistry for serotonin, cholecystokinin, somatostatin, bombesin and calcitonin during different periods of lung development; embryonic, foetal and postnatal. At embryonic period only intraepithelial serotonin-containing cells as sol...

    journal_title:Histology and histopathology

    pub_type: 杂志文章

    doi:

    authors: Balaguer L,Romano J,Ruiz-Pesini P

    更新日期:1992-10-01 00:00:00