Enhanced bioavailability of polyaromatic hydrocarbons in the form of mucin complexes.


:Increasing exposure of biological systems to large amounts of polycyclic aromatic hydrocarbons is of great public concern. Organisms have an array of biological defense mechanisms, and it is believed that mucosal gel (which covers the respiratory system, the gastrointestinal tract, etc.) provides an effective chemical shield against a range of toxic materials. However, in this work, we demonstrate, for the first time, that, upon complexation of polyaromatic hydrocarbons with mucins, enhanced bioavailability and, therefore, toxicity are obtained. This work was aimed to demonstrate how complexation of various highly hydrophobic polycyclic aromatic hydrocarbons with representative mucin glycoprotein could lead to the formation of previously undescribed materials, which exhibit increased toxicity versus pristine polycyclic aromatic hydrocarbons. In the present work, we show that a representative mucin glycoprotein, bovine submaxillary mucin, has impressive and unprecedented capabilities of binding and solubilizing water-insoluble materials in physiological solution. The complexes formed between the mucin and a series of polycyclic aromatic hydrocarbons were comprehensively characterized, and their toxicity was evaluated by both in vivo and in vitro assays. In addition, the bioavailability and membrane-penetration capabilities were tested using an internalization assay. Our results provide, for the first time, evidence of an unknown route by which hydrophobic materials may achieve higher bioavailability, penetrating some of the biological defense systems, in the form of water-soluble complexes with mucosal proteins.


Chem Res Toxicol


Drug E,Landesman-Milo D,Belgorodsky B,Ermakov N,Frenkel-Pinter M,Fadeev L,Peer D,Gozin M




Has Abstract


2011-03-21 00:00:00












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    abstract::Quantitation of DNA adducts could provide critical information on the relationship between exposure to tobacco smoke and cancer risk in smokers. In this study, we developed a robust and sensitive liquid chromatography-tandem mass spectrometry method for the analysis of 4-hydroxy-1-(3-pyridyl)-1-butanone (HPB)-releasin...

    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Stepanov I,Muzic J,Le CT,Sebero E,Villalta P,Ma B,Jensen J,Hatsukami D,Hecht SS

    更新日期:2013-01-18 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Herring JL,Rogstad DK,Sowers LC

    更新日期:2009-06-01 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Gill G,Richter-Rusli AA,Ghosh M,Burrows CJ,Rokita SE

    更新日期:1997-03-01 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Lu K,Cable PH,Abo RP,Ru H,Graffam ME,Schlieper KA,Parry NM,Levine S,Bodnar WM,Wishnok JS,Styblo M,Swenberg JA,Fox JG,Tannenbaum SR

    更新日期:2013-12-16 00:00:00

  • Development of a Fragment-Based in Silico Profiler for Michael Addition Thiol Reactivity.

    abstract::The Adverse Outcome Pathway (AOP) paradigm details the existing knowledge that links the initial interaction between a chemical and a biological system, termed the molecular initiating event (MIE), through a series of intermediate events, to an adverse effect. An important example of a well-defined MIE is the formatio...

    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Ebbrell DJ,Madden JC,Cronin MT,Schultz TW,Enoch SJ

    更新日期:2016-06-20 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Bechtold WE,Strunk MR,Thornton-Manning JR,Henderson RF

    更新日期:1995-03-01 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Lavergne SN,Whitaker P,Peckham D,Conway S,Park BK,Naisbitt DJ

    更新日期:2010-06-21 00:00:00

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    journal_title:Chemical research in toxicology

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    authors: Gibson EG,King MM,Mercer SL,Deweese JE

    更新日期:2016-09-19 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Platt KL,Degenhardt C,Grupe S,Frank H,Seidel A

    更新日期:2002-03-01 00:00:00

  • 16α-Hydroxyestrone: Mass Spectrometry-Based Methodologies for the Identification of Covalent Adducts Formed with Blood Proteins.

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Charneira C,Nunes J,Antunes AMM

    更新日期:2020-08-17 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Balbo S,Villalta PW,Hecht SS

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Ng W,Uetrecht J

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    journal_title:Chemical research in toxicology

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    authors: Dai J,Park G,Wright MW,Adams M,Akman SA,Manderville RA

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    journal_title:Chemical research in toxicology

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    authors: Iyer P,Srinivasan A,Singh SK,Mascara GP,Zayitova S,Sidone B,Fouquerel E,Svilar D,Sobol RW,Bobola MS,Silber JR,Gold B

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Dasaradhi L,Shibutani S

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    pub_type: 杂志文章


    authors: Roy D,Hingerty BE,Shapiro R,Broyde S

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  • Glutathione Conjugation and Protein Adduction by Environmental Pollutant 2,4-Dichlorophenol In Vitro and In Vivo.

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    pub_type: 杂志文章


    authors: Li Q,Li W,Zhao J,Guo X,Zou Q,Yang Z,Tian R,Peng Y,Zheng J

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    journal_title:Chemical research in toxicology

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    authors: Young SA,Capacio BR

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    journal_title:Chemical research in toxicology

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    authors: Wu S,Fisher J,Naciff J,Laufersweiler M,Lester C,Daston G,Blackburn K

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    authors: Greer AK,Madadi NR,Bratton SM,Eddy SD,Mazerska Z,Hendrickson HP,Crooks PA,Radominska-Pandya A

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Zhu M,Spink DC,Yan B,Bank S,DeCaprio AP

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Ivanov SM,Lagunin AA,Pogodin PV,Filimonov DA,Poroikov VV

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Jacob DA,Gibson EG,Mercer SL,Deweese JE

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    pub_type: 杂志文章


    authors: Zhang F,Chen Y,Pisha E,Shen L,Xiong Y,van Breemen RB,Bolton JL

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Streeter AJ,Donovan PJ,Anjo T,Ohannesian L,Sheffels PR,Wu PP,Keefer LK,Andrews AW,Bradford WW 3rd,Reist EJ

    更新日期:1989-05-01 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Weyand EH,Chen YC,Wu Y,Koganti A,Dunsford HA,Rodriguez LV

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Krishnamachari V,Levine LH,Zhou C,Paré PW

    更新日期:2004-06-01 00:00:00

  • Problematic detoxification of estrogen quinones by NAD(P)H-dependent quinone oxidoreductase and glutathione-S-transferase.

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Chandrasena RE,Edirisinghe PD,Bolton JL,Thatcher GR

    更新日期:2008-07-01 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Yang T,Huang Y,Cho BP

    更新日期:2006-02-01 00:00:00

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    journal_title:Chemical research in toxicology

    pub_type: 杂志文章


    authors: Hawkins CL,Davies MJ

    更新日期:2002-01-01 00:00:00