Abstract:
:Recent reports by Galeazzi and co-workers demonstrated the susceptibility of Abeta(1-42) to undergo dityrosine formation via peroxidase-catalyzed tyrosine cross-linking. We have formed dityrosine cross-links in Abeta(1-40) using these enzymatic conditions as well as a copper-H(2)O(2) method. The efficiency of dityrosine cross-link formation is strongly influenced by the aggregation state of Abeta; more dityrosine is formed when copper-H(2)O(2) or horseradish peroxidase-catalyzed oxidation is applied to fibrillar Abeta vs soluble Abeta. Once formed, dityrosine cross-links are susceptible to further oxidative processes and it appears that cross-links formed in soluble Abeta react through these pathways more readily than those formed in fibrillar Abeta. Because preorganization of fibrils affects the efficiency of dityrosine formation, we examined the effect of dityrosine formation upon local peptide conformation by assessing the solution structure of a small dityrosine dimer derived from Abeta(8-14). Two-dimensional (1)H NMR studies of the short dityrosine dimer offer no evidence of structure. Thus, the fibrillar structure of Abeta enhances formation of dityrosine cross-links, but dityrosine cross-links do not seem to enhance local secondary structure.
journal_name
Chem Res Toxicoljournal_title
Chemical research in toxicologyauthors
Yoburn JC,Tian W,Brower JO,Nowick JS,Glabe CG,Van Vranken DLdoi
10.1021/tx025666gsubject
Has Abstractpub_date
2003-04-01 00:00:00pages
531-5issue
4eissn
0893-228Xissn
1520-5010journal_volume
16pub_type
杂志文章abstract::Chemical and structural alterations to lysozyme (LYSO), glucose 6-phosphate dehydrogenase (G6PD), and bovine eye lens proteins (BLP) promoted by peroxyl radicals generated by the thermal decomposition of 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH) under aerobic conditions were investigated. SDS-PAGE analysis of...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
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journal_title:Chemical research in toxicology
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx700404a
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doi:10.1021/tx950168b
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journal_title:Chemical research in toxicology
pub_type: 临床试验,杂志文章
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更新日期:1998-03-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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journal_title:Chemical research in toxicology
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更新日期:2013-03-18 00:00:00
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更新日期:2000-02-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx00023a006
更新日期:1991-09-01 00:00:00
abstract::While xenobiotic carboxylic acids (XCAs) have been studied extensively with respect to their enzymatic conversion to potentially reactive acyl glucuronides with implications to drug induced hepatotoxicity, the formation of xenobiotic-S-acyl-CoA thioesters (xenobiotic-CoAs) have been much less studied in spite of data ...
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更新日期:2013-08-19 00:00:00
abstract::Solutions of synthetic fecapentaene 12 (FP-12) intended for carcinogenicity studies were found to decompose extremely rapidly during customary dosage procedures. Apparent half-lives as short as 15 min were observed. While rates and even the qualitative course of decomposition were surprisingly variable in replicate ex...
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pub_type: 杂志文章
doi:10.1021/tx00009a007
更新日期:1989-05-01 00:00:00
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doi:10.1021/tx400255f
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journal_title:Chemical research in toxicology
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doi:10.1021/acs.chemrestox.5b00398
更新日期:2016-06-20 00:00:00
abstract::Deoxyribose oxidation in DNA represents a biologically important facet of oxidative DNA damage that gives rise to protein-DNA cross-links and base adducts. Toward the goal of quantifying deoxyribose oxidation chemistry in cells, we report a method for the quantification of 3'-phosphoglycolaldehyde (PGA) residues, whic...
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doi:10.1021/tx0341198
更新日期:2003-12-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx00025a023
更新日期:1992-01-01 00:00:00
abstract::The reactions of calf thymus DNA with four 1,3-dialkyl-3-acyltriazenes were studied alone or in the presence of pig liver esterase in pH 7.4 phosphate buffer for varying lengths of time. The best alkylating agent in the absence of esterase was determined to be 1,3-dimethyl-3-carbethoxytriazene (DMC), followed in order...
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pub_type: 杂志文章
doi:10.1021/tx00028a013
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journal_title:Chemical research in toxicology
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更新日期:1996-01-01 00:00:00
abstract::Arsenic is a human carcinogen, causing skin, bladder, and lung cancers. Although arsenic in drinking water affects millions of people worldwide, the mechanism(s) of action by which arsenic causes cancers is not known. Arsenic probably exerts some toxic effects by binding with proteins. However, few experimental data a...
journal_title:Chemical research in toxicology
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更新日期:2003-07-01 00:00:00
abstract::Iron in iron overload disease is present as non-transferrin-bound iron, consisting of iron, citrate, and albumin. We investigated the redox properties of iron citrate by electrochemistry, by the kinetics of its reaction with ascorbate, by ESR, and by analyzing the products of reactions of ascorbate with iron citrate c...
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