当前位置： SCI文献检索 > CHEMICAL RESEARCH IN TOXICOLOGY期刊下所有文献 > Macrophage-mediated endothelial inflammatory responses to airborne particulates: impact of particulate physicochemical properties.

Macrophage-mediated endothelial inflammatory responses to airborne particulates: impact of particulate physicochemical properties.

Abstract：

:Epidemiological studies have implicated a role for airborne particulates of <2.5 microm diameter in the development/exacerbation of chronic cardiopulmonary disease; however, specific pathogenic mechanisms and the etiological significance of particle physicochemical properties remain unresolved. Using a microporous aluminosilicate zeolite Y as a manifold, we have synthesized 1 microm particulates of pure carbon (C), carbon-iron (C/Fe), and carbon-iron/fluoro-aluminum silicate (C-Fe/F-Al-Si). We have used these particulates, as well as coal fly ash (CFA) and diesel exhaust particulates (DEP), to test the hypotheses that human macrophages treated with particulates elaborate proinflammatory cytokines in quantities sufficient to induce endothelial adhesion molecule expression and that macrophage responses to particulate exposure vary as a function of particulate physicochemical properties. Human monocyte-derived macrophages (Mø) were exposed for 24 h to sublethal concentrations of particulates, at which time phagocytosis was evident from optical microscopy. Human arterial, microvascular, or venous endothelial cells (EC) were treated with clarified supernatants recovered from Mø cultures, stained with fluorescein-conjugated mononclonal antibodies specific for endothelial adhesion molecules intercellular adhesion molecule-1, vascular cell adhesion molecule-1, or E-selectin, and assayed by fluorescence flow cytometry. Data generated by these experiments demonstrate that while supernatants of Mø exposed to CFA and C particulates are relatively ineffective, supernatants from DEP, C/Fe, or C-Fe/F-Al-Si strongly induced adhesion molecule expression on EC, responses which were completely attenuated by antibody with blocking specificity for tumor necrosis factor alpha. Because the only difference between C and C/Fe particulates is the presence of surface iron on C/Fe, these findings suggest particulate-induced oxidative stress as a contributing factor in Mø activation and implicate redox active iron as a major determinant of particulate bioreactivity.

journal_name

Chem Res Toxicol

journal_title

Chemical research in toxicology

authors

Kristovich R,Knight DA,Long JF,Williams MV,Dutta PK,Waldman WJ

doi

10.1021/tx049893p

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

1303-12

issue

eissn

0893-228X

issn

1520-5010

journal_volume

pub_type

杂志文章

在线工具

Macrophage-mediated endothelial inflammatory responses to airborne particulates: impact of particulate physicochemical properties.