Abstract:
:Sulfamethoxazole (SMX) is an important antibiotic in the management of patients with cystic fibrosis, but allergic reactions may develop thus restricting therapy. The aim of this study was to utilize drug (metabolite) antigens to diagnose SMX-mediated allergic reactions in patients with cystic fibrosis. Lymphocytes from 2/12 allergic patients were stimulated to proliferate strongly with the SMX metabolite nitroso SMX (SMX-NO). In contrast, responses to SMX were weak. The introduction of an antigen-driven T-cell enrichment step prior to the analysis of proliferation increased the sensitivity of the assay. SMX-NO responses were detected with lymphocytes from all patients with cutaneous signs.
journal_name
Chem Res Toxicoljournal_title
Chemical research in toxicologyauthors
Lavergne SN,Whitaker P,Peckham D,Conway S,Park BK,Naisbitt DJdoi
10.1021/tx100151vsubject
Has Abstractpub_date
2010-06-21 00:00:00pages
1009-11issue
6eissn
0893-228Xissn
1520-5010journal_volume
23pub_type
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