Abstract:
:β-Lapachone (β-lap) is a promising antitumoral agent. DNA base oxidation and alkylation are among the expected damages by β-lap. Herein, we have explored the role that the homologous recombination pathway (HR), a critical DNA repair process in Saccharomyces cerevisiae, has in the cytotoxic profile of β-lap. We have further compared β-lap to the closely related compound menadione and the well-known alkylating agent methyl methanesulfonate (MMS). Surprisingly, we found that β-lap does not trigger HR, as seen for (i) the mutant sensitivity profiles, (ii) concentration-dependent arrest profiles, (iii) absence of nuclear DNA repair factories, and (iv) frequency of recombination between direct repeats.
journal_name
Chem Res Toxicoljournal_title
Chemical research in toxicologyauthors
Quevedo O,García-Luis J,Lorenzo-Castrillejo I,Machín Fdoi
10.1021/tx2004618subject
Has Abstractpub_date
2011-12-19 00:00:00pages
2106-8issue
12eissn
0893-228Xissn
1520-5010journal_volume
24pub_type
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