Abstract：
:Nanoparticles can reach the blood and cause inflammation, suggesting that nanoparticles-endothelial cells interactions may be pathogenically relevant. We evaluated the effect of titanium dioxide nanoparticles (TiO₂) on proliferation, death, and responses related with inflammatory processes such as monocytic adhesion and expression of adhesion molecules (E- and P-selectins, ICAM-1, VCAM-1, and PECAM-1) and with inflammatory molecules (tissue factor, angiotensin-II, VEGF, and oxidized LDL receptor-1) on human umbilical vein endothelial cells (HUVEC). We also evaluated the production of reactive oxygen species, nitric oxide production, and NF-κB pathway activation. Aggregates of TiO₂ of 300 nm or smaller and individual nanoparticles internalized into HUVEC inhibited proliferation strongly and induced apoptotic and necrotic death starting at 5 μg/cm². Besides, TiO₂ induced activation of HUVEC through an increase in adhesion and in expression of adhesion molecules and other molecules involved with the inflammatory process. These effects were associated with oxidative stress and NF-κB pathway activation. In conclusion, TiO₂ induced HUVEC activation, inhibition of cell proliferation with increased cell death, and oxidative stress.
journal_name
Chem Res Toxicoljournal_title
Chemical research in toxicologyauthors
Montiel-Dávalos A,Ventura-Gallegos JL,Alfaro-Moreno E,Soria-Castro E,García-Latorre E,Cabañas-Moreno JG,del Pilar Ramos-Godinez M,López-Marure Rdoi
10.1021/tx200551usubject
Has Abstractpub_date
2012-04-16 00:00:00pages
920-30issue
4eissn
0893-228Xissn
1520-5010journal_volume
25pub_type
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