Fast and simultaneous determination of urinary 8-hydroxy-2'-deoxyguanosine and ten monohydroxylated polycyclic aromatic hydrocarbons by liquid chromatography/tandem mass spectrometry.

abstract：:Oxidative stress is associated with the pathophysiology of many degenerative human diseases, including Alzheimer's disease, atherosclerosis, Parkinson's disease, and cancers. We discovered in our previous study that thioproline (SPro), a proline analogue, is generated in oxidant-exposed cells. With the prior observati...

journal_title：Chemical research in toxicology

authors： Ham YH,Jason Chan KK,Chan W

更新日期：2020-07-20 00:00:00

abstract：:The 2'-deoxyguanosine adduct of the dietary mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) has been synthesized and incorporated into DNA using solid state synthesis technology. The key step to obtaining the C8-dG adduct is a palladium (Xantphos-chelated)-catalyzed N-arylation (Buchwald-Hartwig reactio...

journal_title：Chemical research in toxicology

authors： Bonala R,Torres MC,Iden CR,Johnson F

更新日期：2006-06-01 00:00:00

abstract：:Cr(VI) genotoxicity is caused by products of its reductive metabolism inside the cells. Reactive oxygen species (ROS) and Cr(V,IV) intermediates are potential sources of oxidative damage by Cr(VI). Here, we investigated seven fluorescent probes for the detection of ROS and non-ROS oxidants in Cr(VI) reactions with its...

journal_title：Chemical research in toxicology

authors： DeLoughery Z,Luczak MW,Zhitkovich A

更新日期：2014-05-19 00:00:00

abstract：:The trichothecene mycotoxin T-2 toxin, which is produced by fungi of the Fusarium species, is a worldwide occurring contaminant of cereal based food and feed. The cytotoxic properties of T-2 toxin are already well described with apoptosis being a major mechanism of action in various cell lines as well as in primary ce...

journal_title：Chemical research in toxicology

authors： Weidner M,Lenczyk M,Schwerdt G,Gekle M,Humpf HU

更新日期：2013-03-18 00:00:00

abstract：:The regulation of cellular stress responses to electrophiles and oxidants is mediated by the transcription factor NF-E2-related factor 2 (Nrf2), which, in turn, is regulated by CUL-E3 (CUL3) ligase-mediated ubiquitylation. The Kelch-like ECH-associated protein 1 (Keap1) serves as an adapter between CUL3 and Nrf2. We u...

journal_title：Chemical research in toxicology

authors： Rachakonda G,Xiong Y,Sekhar KR,Stamer SL,Liebler DC,Freeman ML

更新日期：2008-03-01 00:00:00

abstract：:DNA nucleobases are the prime targets for chemical modifications by endogenous and exogenous electrophiles. Alkylation of the N7 position of guanine and adenine in DNA triggers base-catalyzed imidazole ring opening and the formation of N5-substituted formamidopyrimidine (N5-R-FAPy) lesions. Me-FAPy-dG adducts induced ...

journal_title：Chemical research in toxicology

authors： Pujari SS,Tretyakova N

更新日期：2017-01-17 00:00:00

abstract：:Reactive oxygen species can induce the formation of tandem DNA lesions. We recently showed that the treatment of calf thymus DNA with Cu2+/H2O2/ascorbate could result in the efficient formation of a tandem lesion where a 5,6-dihydroxy-5,6-dihydrothymidine (or thymidine glycol) is situated on the 5' side of an 8-oxo-7,...

journal_title：Chemical research in toxicology

authors： Jiang Y,Wang Y,Wang Y

更新日期：2009-03-16 00:00:00

abstract：:Vinyl chloride and acrolein are important industrial chemicals. Both form DNA adducts, vinyl chloride after enzymatic oxidation to chlorooxirane and acrolein by direct reaction. Reaction at the N(2) position of guanine is a major pathway. The resulting 2-oxoethyl and 3-oxopropyl adducts cyclize spontaneously to hydrox...

journal_title：Chemical research in toxicology

authors： Nechev LV,Harris CM,Harris TM

更新日期：2000-05-01 00:00:00

abstract：:Lapatinib (LAP), an oral tyrosine kinase inhibitor for the treatment of metastatic breast cancer, has been associated with idiosyncractic hepatotoxicity. Recent investigations have implicated the importance of P450 3A4/5 enzymes in the formation of an electrophilic quinone imine (LAPQI) metabolite generated through fu...

journal_title：Chemical research in toxicology

authors： Eno MR,El-Gendy Bel-D,Cameron MD

更新日期：2016-05-16 00:00:00

abstract：:Acrolein is a highly toxic environmental pollutant that readily alkylates the epsilon-amino group of lysine residues in proteins. In model systems, such chemistry involves sequential addition of two acrolein molecules to a given nitrogen, forming bis-Michael-adducted species that undergo aldol condensation and dehydra...

journal_title：Chemical research in toxicology

authors： Kaminskas LM,Pyke SM,Burcham PC

更新日期：2005-11-01 00:00:00

abstract：:The cytotoxicity of Al(2)O(3) nanoparticles (NP) at very low exposure levels (1 μg/mL and less) to a dominant bacterial isolate from freshwater (lake water), Bacillus licheniformis, was examined. Sterile lake water was directly used as a test medium or matrix to simulate the freshwater environment. Exposure to 1 μg/mL...

journal_title：Chemical research in toxicology

authors： Pakrashi S,Dalai S,Sabat D,Singh S,Chandrasekaran N,Mukherjee A

更新日期：2011-11-21 00:00:00

abstract：:Small particles of crystalline silicon dioxide (crystallites) are exceptionally toxic. Inhalation of quartz crystallites causes silicosis, a devastating lung disease afflicting miners, particularly coal and stone workers. Poly(vinylpyridine-N-oxide)s (PVPNOs) have been applied in the prevention and treatment of silico...

journal_title：Chemical research in toxicology

authors： Goldstein S,Czapski G,Heller A

更新日期：2006-01-01 00:00:00

abstract：:Glyoxal is generated endogenously from the degradation of glucose and the oxidation of carbohydrates, lipids, and the 2-deoxyribose moieties of DNA. Glyoxal is also widely used in industry and is present in cigarette smoke and food. Glyoxal can conjugate with nucleobases and proteins to give advanced glycation end pro...

journal_title：Chemical research in toxicology

authors： Wang H,Cao H,Wang Y

更新日期：2010-01-01 00:00:00

abstract：:Platinum group metals (PGMs) is a group of metals that include platinum, palladium, rhodium, ruthenium, iridium, and osmium. Occupational respiratory exposure to platinum has been reported since 1945, but studies investigating occupational exposure to palladium, rhodium, ruthenium, iridium, and osmium are scarce. This...

journal_title：Chemical research in toxicology

authors： Linde SJL,Franken A,du Plessis JL

更新日期：2017-10-16 00:00:00

abstract：:Drug-induced kidney injury (DIKI) is a common toxicity observed in pharmaceutical development. We demonstrated the use of label-free liquid chromatography-mass spectrometry (LC-MS) and multiplex liquid chromatography-single reaction monitoring (LC-SRM) as practical extensions of standard immunoassay based safety bioma...

journal_title：Chemical research in toxicology

authors： Chen L,Smith J,Mikl J,Fryer R,Pack F,Williams BJ,Phillips JA,Papov VV Jr

更新日期：2017-10-16 00:00:00

abstract：:The bifunctional alkylating agent, melphalan, forms adducts on DNA that are recognized by two previously described monoclonal antibodies, MP5/73 and Amp4/42. Immunoreactivity to MP5/73 was lost when alkylated DNA was exposed to alkaline pH, while Amp4/42 only recognized the structures formed after the alkali treatment...

journal_title：Chemical research in toxicology

authors： McCartney H,Martin AM,Middleton PG,Tilby MJ

更新日期：2001-01-01 00:00:00

abstract：:Previous work has shown that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes porphyria, enhanced by iron, in C57BL/6J mice with marked accumulation in the liver of uroporphyrin I and III isomers and heptacarboxylic acid III and is one model of human porphyria cutanea tarda. Preliminary examination by HPLC also indic...

journal_title：Chemical research in toxicology

authors： Lim CK,Danton M,Clothier B,Smith AG

更新日期：2006-12-01 00:00:00

abstract：:Exposures to sulfur mustard (HD; bis(2-chloroethyll) sulfide) are well-known to result in the formation of adducts with free aspartate and glutamate residues of plasma proteins (Lawrence, R. J., Smith, J. R., and Capacio, B. R. 2008 32, (1), 31-36). A modified version of the analytical method reported previously for t...

journal_title：Chemical research in toxicology

authors： Young SA,Capacio BR

更新日期：2020-07-20 00:00:00

abstract：:A series of alpha-(X-substituted-methyl)-gamma,gamma-dimethyl-gamma-butyrolactones (series 1), a series of alpha-(2-X-substituted-ethyl)-gamma,gamma-dimethyl-gamma-butyrolactones (series 2), where X is a leaving group, and the compound alpha-(3-bromopropyl)-gamma,gamma-butyrolactone (3) were synthesized. Their reactio...

journal_title：Chemical research in toxicology

authors： Franot C,Roberts DW,Smith RG,Basketter DA,Benezra C,Lepoittevin JP

更新日期：1994-05-01 00:00:00

abstract：:The rat esophagus is strikingly sensitive to tumor induction by nitrosamines, and it has been hypothesized that this tissue contains cytochrome P450 enzymes (P450s) which catalyze the metabolic activation of these carcinogens. The metabolic capacity of the esophagus is not well characterized. In the study described he...

journal_title：Chemical research in toxicology

authors： von Weymarn LB,Murphy SE

更新日期：2001-10-01 00:00:00

abstract：:Implementation of the Clinical Data Interchange Standards Consortium (CDISC)'s Standard for Exchange of Nonclinical Data (SEND) by the United States Food and Drug Administration Center for Drug Evaluation and Research (US FDA CDER) has created large quantities of SEND data sets and a tremendous opportunity to apply la...

journal_title：Chemical research in toxicology

authors： Carfagna MA,Anderson J,Eley C,Fukushima T,Horvath J,Houser W,Larsen B,Page T,Russo D,Sloan C,Snyder K,Thompson R,Ullmann G,Whittaker M

更新日期：2020-12-16 00:00:00

abstract：:Epidemiological studies have implicated a role for airborne particulates of <2.5 microm diameter in the development/exacerbation of chronic cardiopulmonary disease; however, specific pathogenic mechanisms and the etiological significance of particle physicochemical properties remain unresolved. Using a microporous alu...

journal_title：Chemical research in toxicology

authors： Kristovich R,Knight DA,Long JF,Williams MV,Dutta PK,Waldman WJ

更新日期：2004-10-01 00:00:00

abstract：:Thioureas are oxygenated by flavin-containing monooxygenases (FMOs), forming reactive sulfenic and/or sulfinic acids. Sulfenic acids can reversibly react with GSH and drive oxidative stress through a redox cycle. For this reason, thiourea S-oxygenation is an example of FMO-dependent bioactivation of a xenobiotic. Func...

journal_title：Chemical research in toxicology

authors： Henderson MC,Krueger SK,Stevens JF,Williams DE

更新日期：2004-05-01 00:00:00

abstract：:Autophagy is a "self-eating" destructive process that eliminates damaged organelles to maintain cellular homeostasis. Polychlorinated biphenyls (PCBs) are one of the most infamous industrial pollutants, which are ubiquitous in nature. In the present study, we found that an active, quinone-type PCB metabolite (PCB29-pQ...

journal_title：Chemical research in toxicology

authors： Shi Q,Song X,Liu Z,Wang Y,Wang Y,Fu J,Su C,Xia X,Song E,Song Y

更新日期：2016-07-18 00:00:00

abstract：:Prodrugs of 1,2-bis(methylsulfonyl)-1-(2-chloroethyl)hydrazine (90CE) are promising anticancer agents. The 90CE moiety is a readily latentiated, short-lived (t1/2 ∼ 30 s) chloroethylating agent that can generate high yields of oxophilic electrophiles responsible for the chloroethylation of the O-6 position of guanine ...

journal_title：Chemical research in toxicology

authors： Penketh PG,Patridge E,Shyam K,Baumann RP,Zhu R,Ishiguro K,Sartorelli AC

更新日期：2014-08-18 00:00:00

abstract：:Marine toxins such as brevetoxins and ciguatoxins are produced by dinoflagellates and can accumulate in seafood. These toxins affect humans through seafood consumption. Intoxication is mainly characterized by gastrointestinal and neurological disorders and, in most severe cases, by cardiovascular problems. To prevent ...

journal_title：Chemical research in toxicology

authors： Louzao MC,Vieytes MR,Yasumoto T,Botana LM

更新日期：2004-04-01 00:00:00

abstract：:The mechanism of DNA adduct formation by acroleins substituted with good leaving groups in the beta-position was investigated using [3-2H]-3-(p-nitrophenoxy)acrolein (1). Reaction of 1 with guanosine at acidic pH produced 9-beta-D-ribofuranosylpyrimido[1,2-alpha]purin-10(3H)-one containing equal amounts of deuterium a...

journal_title：Chemical research in toxicology

authors： Reddy GR,Marnett LJ

更新日期：1996-01-01 00:00:00

abstract：:The major plant growth regulator ethephon degrades to ethylene and phosphate in aqueous solutions and plants and is spontaneously activated to a butyrylcholinesterase (BChE) inhibitor in alkaline solutions and animal tissues. In the present (31)P NMR kinetic study of the reactions of ethephon in pH 7.4 carbonate buffe...

journal_title：Chemical research in toxicology

authors： Lantz SR,Casida JE

更新日期：2013-09-16 00:00:00

abstract：:Peroxynitrite (ONOO-), the reaction product of superoxide (O2.-) and nitric oxide (.NO), nitrates tyrosine and other phenolics. We report herein that tryptophan is also nitrated by peroxynitrite in the absence of transition metals to one predominant isomer of nitrotryptophan, as determined from spectral characteristic...

journal_title：Chemical research in toxicology

authors： Alvarez B,Rubbo H,Kirk M,Barnes S,Freeman BA,Radi R

更新日期：1996-03-01 00:00:00

abstract：:The reaction of the antitumor agent leinamycin with thiols converts this natural product into an episulfonium ion that alkylates the N7-position of guanine residues in double-stranded DNA. It is reported here that depurination of this adduct is unusually facile, occurring with a half-life of about 3.5 h at pH 7 and 37...

journal_title：Chemical research in toxicology

authors： Nooner T,Dutta S,Gates KS

更新日期：2004-07-01 00:00:00