Abstract：
:Finger 3 of transcription factor IIIA of Xenopus laevis was synthesized and constituted with Zn(2+) or Cd(2+). The C-block element of the internal control region of the promoter of the 5S rRNA gene binds to the Zn-F3 and Cd-F3 with dissociation constants of 2.6 x 10(-5) and 1.5 x 10(-4) M, respectively. According to NMR spectroscopy, Zn-F3, as well as Cd-F3, exists as a conformational equilibrium that is not susceptible to structural analysis by NMR methods. To restrict the observed conformational flexibility, a mutant F3 (mF3), which differs from F3 in the number and type of amino acids between the cysteine and the histidine ligands, was synthesized. The affinity of Zn-mF3 for the C-block DNA was greatly reduced relative to Zn-F3. Nevertheless, the metal ion dissociation constants of the Zn- and Cd-mF3 complexes remain similar to those of the native structures at 4.5 x 10(-9) and 3.2 x 10(-8) M, respectively. Zn-mF3 is more thermally stable than Cd-mF3, but both adopt similar conformations according to two-dimensional (1)H NMR spectroscopy. Each peptide displays a betabetaalpha fold for its backbone that is typical of this class of zinc finger domains. The(113)Cd ion in (113)Cd-mF3 is coupled to the protons of two cysteine and two histidine residues and characterized by a chemical shift of 567 ppm.
journal_name
Chem Res Toxicoljournal_title
Chemical research in toxicologyauthors
Krepkiy D,Försterling FH,Petering DHdoi
10.1021/tx030057+subject
Has Abstractpub_date
2004-07-01 00:00:00pages
863-70issue
7eissn
0893-228Xissn
1520-5010journal_volume
17pub_type
杂志文章abstract：:The environmental contaminant benzo[c]phenanthrene (B[c]Ph) has weak carcinogenic activity in rodent bioassays; however, the fjord region diol epoxides of B[c]Ph, B[c]Ph-3,4-diol 1,2-epoxides (B[c]PhDE), are potent carcinogens. To determine the role of cytochrome P450 isozymes in the activation of B[c]Ph in MCF-7 cell...
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更新日期：1992-03-01 00:00:00
