Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells.


:Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to prevent transmission, vaccine-induced antibodies may need to block viral infection of epithelial cells of the oral or genital mucosa. We found that gH/gL/UL128-131 is necessary for efficient viral entry into epithelial cells derived from oral and genital mucosa, that short peptides from UL130 and UL131 elicit high titer neutralizing antibodies in rabbits, and that such antibodies neutralize viral entry into epithelial cells derived from these relevant tissues. These results suggest that single subunits or peptides may be sufficient to elicit potent epithelial entry neutralizing responses and that secretory antibodies to such neutralizing epitopes have the potential to provide sterilizing immunity by blocking initial mucosal infection.






Saccoccio FM,Sauer AL,Cui X,Armstrong AE,Habib el-SE,Johnson DC,Ryckman BJ,Klingelhutz AJ,Adler SP,McVoy MA




Has Abstract


2011-03-24 00:00:00
















  • Noninvasive vaccination: from the perspective of sonoporation.

    abstract::Previous noninvasive vaccine submission approaches including respiratory tract-based methods are limited to specific pathogens. Recent developments on ultrasound biology suggest that ultrasound-mediated molecule submission (sonoporation) could be an effective noninvasive approach for gene therapy as well as drug submi...


    pub_type: 信件


    authors: Yuan TF

    更新日期:2008-08-05 00:00:00

  • The outer membrane protein X from Escherichia coli exhibits immune properties.

    abstract::Outer membrane proteins (OMP) are expressed in Gram-negative bacterial cell wall. OmpA from Klebsiella pneumoniae (KpOmpA) has been shown to bind and to activate selectively antigen presenting cells (APCs), eliciting protective CTL responses. In this study, we investigated whether OmpX, another member of the OMP famil...


    pub_type: 杂志文章


    authors: Maisnier-Patin K,Malissard M,Jeannin P,Haeuw JF,Corbière JC,Hoeffel G,Gauchat JF,Nguyen T,Saez JM,Delneste Y

    更新日期:2003-09-08 00:00:00

  • Catalase immunization from Pseudomonas aeruginosa enhances bacterial clearance in the rat lung.

    abstract::Pseudomonas aeruginosa is a common cause of infection in immunocompromised patients and is the major contributor to morbidity in individuals with cystic fibrosis (CF). The antibiotic resistance shown by this pathogen and morbidity in patients with chronic infection has encouraged investigations into the development of...


    pub_type: 杂志文章


    authors: Thomas LD,Dunkley ML,Moore R,Reynolds S,Bastin DA,Kyd JM,Cripps AW

    更新日期:2000-09-15 00:00:00

  • Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice.

    abstract::Intramuscular immunization of mice with DNA cocktail vaccines, comprising potential protective antigens P36, P46, NrdF, and P97or P97R1 of Mycoplasma hyopneumoniae, induced strong Th1-polarized immune responses against each antigen, with only P46 eliciting a serum IgG response. Subcutaneous immunization with protein c...


    pub_type: 杂志文章


    authors: Chen AY,Fry SR,Daggard GE,Mukkur TK

    更新日期:2008-08-12 00:00:00

  • Effect of 10-valent pneumococcal conjugate vaccine on nasopharyngeal carriage of Streptococcus pneumoniae and Haemophilus influenzae among children in São Paulo, Brazil.

    abstract::In March 2010, Brazil introduced the 10-valent pneumococcal conjugate vaccine (PCV10) in the routine infant immunization program using a 4-dose schedule and catch-up for children <23months. We investigated PCV10 effect on nasopharyngeal carriage with vaccine-type Streptococcus pneumoniae (Spn) and non-typeable Haemoph...


    pub_type: 杂志文章


    authors: Brandileone MC,Zanella RC,Almeida SCG,Brandao AP,Ribeiro AF,Carvalhanas TMP,Sato H,Andrade AL,Verani JR,Pneumococcal Carriage Study Group.

    更新日期:2016-11-04 00:00:00

  • Singaporean men's knowledge of cervical cancer and human papillomavirus (HPV) and their attitudes towards HPV vaccination.

    abstract::Little is known of men's knowledge of cervical cancer and its links with human papillomavirus (HPV), or of their attitudes and beliefs about HPV vaccination. This is despite men's sexual behaviour contributing to HPV transmission and their potential role in deciding whether their children are vaccinated against HPV. T...


    pub_type: 杂志文章


    authors: Pitts M,Smith A,Croy S,Lyons A,Ryall R,Garland S,Wong ML,Hseon TE

    更新日期:2009-05-14 00:00:00

  • Human schistosomiasis: potential consequences of vaccination.

    abstract::The potential outcomes of a Phase II schistosomiasis vaccine trial are explored using mathematical models which assume some natural immunity and a vaccine providing partial protection for a limited time. Analyses suggest that vaccination may have only a limited impact on life-long cumulative worm burdens and may lead ...


    pub_type: 杂志文章


    authors: Woolhouse ME

    更新日期:1995-08-01 00:00:00

  • A phase I evaluation of inactivated influenza A/H5N1 vaccine administered by the intradermal or the intramuscular route.

    abstract::In a phase I clinical trial, one hundred healthy young adults were randomized to receive two doses 28 days apart of an inactivated, subvirion vaccine containing 15 or 45microg of influenza A/H5N1 hemagglutinin (HA) by the intramuscular (IM) route, or 3 or 9microg of H5 HA by the intradermal(ID) route. Seventy-seven su...


    pub_type: 杂志文章,随机对照试验


    authors: Patel SM,Atmar RL,El Sahly HM,Cate TR,Keitel WA

    更新日期:2010-04-09 00:00:00

  • Neuraminidase inhibiting antibody responses in pigs differ between influenza A virus N2 lineages and by vaccine type.

    abstract::The neuraminidase (NA) protein of influenza A viruses (IAV) has important functional roles in the viral replication cycle. Antibodies specific to NA can reduce viral replication and limit disease severity, but are not routinely measured. We analyzed NA inhibiting (NI) antibody titers in serum and respiratory specimens...


    pub_type: 杂志文章


    authors: Sandbulte MR,Gauger PC,Kitikoon P,Chen H,Perez DR,Roth JA,Vincent AL

    更新日期:2016-07-19 00:00:00

  • Vaccine effectiveness against severe laboratory-confirmed influenza in children: results of two consecutive seasons in Italy.

    abstract:OBJECTIVE:To evaluate the effectiveness of seasonal influenza vaccine in preventing Emergency Department (ED) visits and hospitalisations for influenza like illness (ILI) in children. METHODS:We conducted a test negative case-control study during the 2011-2012 and 2012-2013 influenza seasons. Eleven paediatric hospita...


    pub_type: 杂志文章


    authors: Menniti-Ippolito F,Da Cas R,Traversa G,Santuccio C,Felicetti P,Tartaglia L,Trotta F,Di Pietro P,Barabino P,Renna S,Riceputi L,Tovo PA,Gabiano C,Urbino A,Baroero L,Le Serre D,Virano S,Perilongo G,Daverio M,Gnoato E,

    更新日期:2014-07-31 00:00:00

  • The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation.

    abstract::We investigated the influence of the site of administration of DNA vaccine on the induced immune response. DNA vaccines were administered by electroporation at three different sites: tibial cranial muscle, abdominal skin and ear pinna. Aiming to draw general conclusions about DNA vaccine delivery, we successively used...


    pub_type: 杂志文章


    authors: Vandermeulen G,Vanvarenberg K,De Beuckelaer A,De Koker S,Lambricht L,Uyttenhove C,Reschner A,Vanderplasschen A,Grooten J,Préat V

    更新日期:2015-06-22 00:00:00

  • Oral polio vaccination and low case fatality at the paediatric ward in Bissau, Guinea-Bissau.

    abstract::Oral polio vaccine (OPV) and diphtheria-tetanus-pertussis (DTP) vaccines are given simultaneously in routine immunisation programmes in developing countries. It is therefore difficult to determine the separate effects of these vaccines on survival. We used the shortage of DTP vaccine in Bissau to examine the impact of...


    pub_type: 杂志文章


    authors: Aaby P,Rodrigues A,Biai S,Martins C,Veirum JE,Benn CS,Jensen H

    更新日期:2004-08-13 00:00:00

  • Identifying barriers and drivers to vaccination: A qualitative interview study with health workers in the Federation of Bosnia and Herzegovina.

    abstract:BACKGROUND:Vaccination coverage in Bosnia and Herzegovina has been declining over recent years. A World Health Organization Tailoring Immunization Programmes (TIP) project is underway to gain insights into the underlying reasons for this, to develop tailored interventions. As part of TIP, this study aimed to investigat...


    pub_type: 杂志文章


    authors: Musa S,Skrijelj V,Kulo A,Habersaat KB,Smjecanin M,Primorac E,Becirovic D,Jackson C

    更新日期:2020-02-18 00:00:00

  • Four year immunogenicity of the RTS,S/AS02(A) malaria vaccine in Mozambican children during a phase IIb trial.

    abstract::Previous studies with the malaria vaccine RTS,S/AS02(A) in young children in a malaria endemic area of Mozambique have shown it to have a promising safety profile and to reduce the risk of Plasmodium falciparum infection and disease. In this study, we assessed the antibody responses to the P. falciparum and hepatitis ...


    pub_type: 杂志文章,随机对照试验


    authors: Aide P,Dobaño C,Sacarlal J,Aponte JJ,Mandomando I,Guinovart C,Bassat Q,Renom M,Puyol L,Macete E,Herreros E,Leach A,Dubois MC,Demoitie MA,Lievens M,Vekemans J,Loucq C,Ballou WR,Cohen J,Alonso PL

    更新日期:2011-08-11 00:00:00

  • Improved cell mediated immune responses after successful re-vaccination of non-responders to the hepatitis B virus surface antigen (HBsAg) vaccine using the combined hepatitis A and B vaccine.

    abstract::We successfully re-vaccinated hepatitis B virus (HBV) vaccine non-responders using a double dose of the combined hepatitis A virus (HAV) and HBV vaccine. The hope was to improve priming of hepatitis B surface antigen (HBsAg)-specific cell mediated immune response (CMI) by an increased antigen dose and a theoretical ad...


    pub_type: 杂志文章


    authors: Nyström J,Cardell K,Björnsdottir TB,Fryden A,Hultgren C,Sällberg M

    更新日期:2008-11-05 00:00:00

  • Review of prescribing information for influenza vaccines for pregnant and lactating women.

    abstract::Information provided by most influenza vaccine manufacturers do not reflect the recommendations of WHO and/or national public health advisory groups with regard to the use of influenza vaccines in pregnant or lactating women. The majority of vaccines contain precautionary language which could discourage use in pregnan...


    pub_type: 杂志文章,评审


    authors: Proveaux T,Lambach P,Ortiz JR,Hombach J,Halsey NA

    更新日期:2016-10-26 00:00:00

  • HBV vaccination in hyperendemic remote tribal areas in India.

    abstract::Randomly selected 200 subjects were tested for quantitative hepatitis B surface antibody (HBsAb) estimation in a remote tribal area of Arunachal Pradesh, India to measure the protective immunity after vaccination in the backdrop of hyperendemic HBV infection. About 64.6 and 40.0% had HBsAb above 10 mIU/ml in the vacci...


    pub_type: 信件


    authors: Borkakoty B,Mahanta J,Biswas D

    更新日期:2007-12-05 00:00:00

  • Immunological cross-reactivity against a drug mutated HIV-1 protease epitope after DNA multi-CTL epitope construct immunization.

    abstract::Epitopes in HIV polymerase were analyzed by peptide binding to human leukocyte antigen (HLA) A0201 molecules, the most frequent HLA class in the Caucasian population. We found that HIV-1 protease peptides representing both the wild type and anticipated drug resistance variants of the sequence bound well to HLA-A0201. ...


    pub_type: 杂志文章


    authors: Boberg A,Sjöstrand D,Rollman E,Hinkula J,Zuber B,Wahren B

    更新日期:2006-05-22 00:00:00

  • Evaluation of 12 stabilizers in a developed attenuated Salmonella Enteritidis vaccine.

    abstract::The development of a stable live attenuated Salmonella Enteritidis (SE) vaccine, resisting heat stress during transportation and storage in unequipped tropical and subtropical zones of the world, is highly recommended. Twelve stabilizers were individually supplemented into a 9 ml volume of sterile distilled water resu...


    pub_type: 杂志文章


    authors: Barbour EK,Abdelnour A,Jirjis F,Faroon O,Farran MT

    更新日期:2002-05-22 00:00:00

  • New strategies for closing the gap of measles susceptibility in infants: towards vaccines compatible with current vaccination schedules.

    abstract::Peptides representing epitopes of the measles virus glycoproteins have been designed to induce neutralizing and protective antibodies. Those that escape recognition by passively acquired anti-whole virus antibodies could potentially be used as components of a 'pre-vaccine' that could be given during early childhood ir...


    pub_type: 杂志文章,评审


    authors: El Kasmi KC,Muller CP

    更新日期:2001-03-21 00:00:00

  • Evaluation of protection in a mouse model after vaccination with Mycobacterium avium subsp. paratuberculois protein cocktails.

    abstract::Whole-cell vaccines successfully reduce signs of clinical disease and fecal shedding of Mycobacterium avium subsp. paratuberculosis (MAP), however, these vaccines have some limitations. The present study was conducted to identify MAP proteins that might be candidates for the development of an improved vaccine. MAP pro...


    pub_type: 杂志文章


    authors: Stabel JR,Barnhill A,Bannantine JP,Chang YF,Osman MA

    更新日期:2012-12-17 00:00:00

  • Understanding the reactogenicity of 4CMenB vaccine: Comparison of a novel and conventional method of assessing post-immunisation fever and correlation with pre-release in vitro pyrogen testing.

    abstract:BACKGROUND:Better understanding of vaccine reactogenicity is crucial given its potential impact upon vaccine safety and acceptance. Here we report a comparison between conventional and novel (continuous) methods of monitoring temperature and evaluate any association between reactogenicity and the monocyte activation te...


    pub_type: 杂志文章


    authors: Valente Pinto M,Davis K,Andrews N,Goldblatt D,Borrow R,Southern J,Nordgren IK,Vipond C,Plested E,Miller E,Snape MD

    更新日期:2020-11-17 00:00:00

  • Vaccination of sheep with Quil-A® adjuvant expands the antibody repertoire to the Fasciola MF6p/FhHDM-1 antigen and administered together impair the growth and antigen release of flukes.

    abstract::Fasciolosis continues to be a major cause of economic losses in the livestock industry and a growing threat to humans. The limited spectrum of effective anthelmintics and the appearance of resistances urge the need for developing an effective vaccine. Most studies have been focused on the use of TH1-polarizing adjuvan...


    pub_type: 杂志文章


    authors: Orbegozo-Medina RA,Martínez-Sernández V,González-Warleta M,Castro-Hermida JA,Mezo M,Ubeira FM

    更新日期:2018-04-05 00:00:00

  • Effect of emergency FMD vaccine antigen payload on protection, sub-clinical infection and persistence following direct contact challenge of cattle.

    abstract::Previous work, in sheep vaccinated with emergency foot-and-mouth disease (FMD) vaccine, indicated the benefit of increasing the antigen payload in inhibiting local virus replication and consequently persistence following an indirect aerosol challenge with a virus homologous to the vaccine strain. The work presented he...


    pub_type: 杂志文章


    authors: Cox SJ,Voyce C,Parida S,Reid SM,Hamblin PA,Hutchings G,Paton DJ,Barnett PV

    更新日期:2006-04-12 00:00:00

  • Nasal vaccination induces the ability to eliminate Candida colonization without influencing the pre-existing antigen-specific IgE Abs: a possibility for the control of Candida-related atopic dermatitis.

    abstract::In some cases of atopic dermatitis (AD), a possible pathological contribution to disease development by Candida albicans (C. albicans) has been suggested. AD patients with severe symptoms showing positive capsulated hydrolic carrier polymer radioallergosorbent test (CAP-RAST) against C. albicans demonstrated significa...


    pub_type: 杂志文章


    authors: Suenobu N,Kweon MN,Kiyono H

    更新日期:2002-07-26 00:00:00

  • Critical issues in cancer vaccine trial design.

    abstract::As the clinical experience with cancer vaccines and cancer immunotherapy increases, there are important lessons that can be learned from the successes and failures of past trials. Many lessons affect the design and conduct of clinical trials themselves. Appropriate patient selection, clinical trial design, immunologic...


    pub_type: 杂志文章,评审


    authors: Clifton GT,Kohrt HE,Peoples GE

    更新日期:2015-12-16 00:00:00

  • SHIV virus-like particles bind and activate human dendritic cells.

    abstract::We previously demonstrated that mucosal immunization with SHIV virus-like particles (VLPs) was able to induce strong humoral and cellular immune responses against HIV envelope protein (Env). To understand the mechanism for such enhanced immune responses, we studied the interaction between VLPs and dendritic cells (DCs...


    pub_type: 杂志文章


    authors: Zhang R,Li M,Chen C,Yao Q

    更新日期:2004-11-25 00:00:00

  • The Health Belief Model in predicting healthcare workers' intention for influenza vaccine uptake in Jordan.

    abstract:BACKGROUND:Theories of health behavior change are being inadequately adopted to understand the reasons behind low influenza vaccination rates among healthcare workers (HCWs). The Theory of Planned Behavior (TPB) is being used to predict intention-behavior relationship while the Health Belief Model (HBM) is being employ...


    pub_type: 杂志文章


    authors: Alhalaseh L,Fayoumi H,Khalil B

    更新日期:2020-10-27 00:00:00

  • Vaccination recommendations for patients with neuromuscular disease.

    abstract::Neuromuscular diseases (NMDs) encompass a broad spectrum of conditions. Because infections may be relevant to the final prognosis of most NMDs, vaccination appears to be the simplest and most effective solution for protecting NMD patients from vaccine-preventable infections. However, very few studies have evaluated th...


    pub_type: 杂志文章


    authors: Esposito S,Bruno C,Berardinelli A,Filosto M,Mongini T,Morandi L,Musumeci O,Pegoraro E,Siciliano G,Tonin P,Marrosu G,Minetti C,Servida M,Fiorillo C,Conforti G,Scapolan S,Ansaldi F,Vianello A,Castaldi S,Principi N,T

    更新日期:2014-10-14 00:00:00

  • Induction of a cellular immune response to a defined T-cell epitope as an insert in the flagellin of a live vaccine strain of Salmonella.

    abstract::Attenuated strains of Salmonella have been used as vaccines to deliver heterologous antigens mainly to generate a humoral immune response. However, little is known about their ability to induce a cell-mediated immune response to the T-cell epitopes of another infectious agent or how optimally to deliver these epitopes...


    pub_type: 杂志文章


    authors: Verma NK,Ziegler HK,Stocker BA,Schoolnik GK

    更新日期:1995-02-01 00:00:00