Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells.


:Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to prevent transmission, vaccine-induced antibodies may need to block viral infection of epithelial cells of the oral or genital mucosa. We found that gH/gL/UL128-131 is necessary for efficient viral entry into epithelial cells derived from oral and genital mucosa, that short peptides from UL130 and UL131 elicit high titer neutralizing antibodies in rabbits, and that such antibodies neutralize viral entry into epithelial cells derived from these relevant tissues. These results suggest that single subunits or peptides may be sufficient to elicit potent epithelial entry neutralizing responses and that secretory antibodies to such neutralizing epitopes have the potential to provide sterilizing immunity by blocking initial mucosal infection.






Saccoccio FM,Sauer AL,Cui X,Armstrong AE,Habib el-SE,Johnson DC,Ryckman BJ,Klingelhutz AJ,Adler SP,McVoy MA




Has Abstract


2011-03-24 00:00:00
















  • Impact of universal vaccination programmes on the epidemiology of hepatitis B: 10 years of experience in Italy.

    abstract::Ten years have elapsed since routine vaccination of infants and of 12-year-old adolescent was implemented in Italy. In this period, evidence has accumulated on the epidemiological impact of universal immunisation. Coverage is on average >90% and is >or=95% in many areas of the country. Incidence of acute hepatitis B, ...


    pub_type: 杂志文章


    authors: Bonanni P,Pesavento G,Bechini A,Tiscione E,Mannelli F,Benucci C,Nostro AL

    更新日期:2003-01-30 00:00:00

  • Molecular characterisation of pneumococcal serotype 16F: Established predominant carriage and otitis media serotype in the 7vPCV era.

    abstract::Young Australian Aboriginal children in remote communities experience very high rates of pneumococcal carriage and otitis media. Prior to introduction of the 7-valent pneumococcal conjugate vaccine (7vPCV, Prevenar), serotype 16F was an important type found in nasal and ear discharge swabs. Since commencement of pneum...


    pub_type: 杂志文章


    authors: Marsh RL,Smith-Vaughan H,Beissbarth J,Hare K,Kennedy M,Wigger C,Mellon G,Stubbs E,Gadil JR,Pettit A,Mackenzie G,Tipakalippa P,Morris PS,Leach AJ

    更新日期:2007-03-22 00:00:00

  • Intranasal inoculate of influenza virus vaccine against lethal virus challenge.

    abstract::Vaccine adjuvants are essential for enhancing immune responses during vaccination. However, only a limited number of safe and effective adjuvants, especially mucosal adjuvants, are available for use in vaccines. The development of a practically applicable mucosal adjuvant is therefore urgently needed. Here, we showed ...


    pub_type: 杂志文章


    authors: Fan X,Su Q,Qiu F,Yi Y,Shen L,Jia Z,Liang P,Zou Y,Bi S

    更新日期:2018-07-05 00:00:00

  • A discrete computer model of the immune system reveals competitive interactions between the humoral and cellular branch and between cross-reacting memory and naïve responses.

    abstract::In an agent-based computer model, we simulate the formation and recall of anti-virus immunological memory. Specifically we try to predict what will happen, both to the response and to memory, when the second infecting virus is partly different from the first one, and when the cross-reactivity of the two branches of th...


    pub_type: 杂志文章


    authors: Cheng Y,Ghersi D,Calcagno C,Selin LK,Puzone R,Celada F

    更新日期:2009-02-05 00:00:00

  • Factors influencing frontline health service providers' likelihood to recommend a future, preventive HIV vaccine to key populations in Karnataka, south India.

    abstract::The HIV epidemic in the south Indian state of Karnataka disproportionately burdens key populations of men who have sex with men and female sex workers. Despite having successfully reduced HIV incidence among certain key populations through the use of targeted intervention, India's HIV epidemic remains one of its great...


    pub_type: 杂志文章


    authors: McClarty LM,Lorway RR,Ramanaik S,Wylie J,Becker ML

    更新日期:2015-01-29 00:00:00

  • Estimating the vaccine-preventable burden of hospitalized pneumonia among young Mozambican children.

    abstract::Polysaccharide-protein conjugate vaccines against Haemophilus influenzae type b (Hib) and Streptococcus pneumoniae have proven efficacy against radiologically confirmed pneumonia. Measurement of pneumonia incidence provides a platform to estimate of the vaccine-preventable burden. Over 24 months, we conducted surveill...


    pub_type: 杂志文章


    authors: Roca A,Sigaúque B,Quintó L,Morais L,Berenguera A,Corachan M,Ribó JL,Naniche D,Bassat Q,Sacoor Ch,Nhalungo D,Macete E,Schuchat A,Soriano-Gabarró M,Flannery B,Alonso PL

    更新日期:2010-07-05 00:00:00

  • Adverse events following immunization in patients with primary immunodeficiencies.

    abstract:BACKGROUND:Adverse events following immunization (AEFI) requires special consideration in patients with primary immunodeficiency diseases (PID) because they may represent a "red flag" for the initial diagnosis and may cause disease complications. Therefore, the definition of appropriate vaccination schemes is a major i...


    pub_type: 杂志文章


    authors: Sarmiento JD,Villada F,Orrego JC,Franco JL,Trujillo-Vargas CM

    更新日期:2016-03-18 00:00:00

  • fH-dependent complement evasion by disease-causing meningococcal strains with absent fHbp genes or frameshift mutations.

    abstract::Meningococci bind human fH to down-regulate complement, which enhances survival of the bacteria in serum. A major fH ligand is the vaccine candidate, factor H-binding protein (fHbp). Although fHbp has been considered an essential meningococcal virulence factor, rarely, invasive isolates with absent fHbp genes or frame...


    pub_type: 杂志文章


    authors: Giuntini S,Vu DM,Granoff DM

    更新日期:2013-08-28 00:00:00

  • The comparative evaluation of expanded national immunization policies in Korea using an analytic hierarchy process.

    abstract::The purpose of this paper is to propose new evaluation criteria and an analytic hierarchy process (AHP) model to assess the expanded national immunization programs (ENIPs) and to evaluate two alternative health care policies. One of the alternative policies is that private clinics and hospitals would offer free vaccin...


    pub_type: 杂志文章,评审


    authors: Shin T,Kim CB,Ahn YH,Kim HY,Cha BH,Uh Y,Lee JH,Hyun SJ,Lee DH,Go UY

    更新日期:2009-01-29 00:00:00

  • Evaluation of avian influenza virus isolated from ducks as a potential live vaccine candidate against novel H7N9 viruses.

    abstract::Recent outbreaks of a novel H7N9 avian influenza virus in humans in China raise pandemic concerns and underscore an urgent need to develop effective vaccines. Theoretically, live influenza vaccines are of multiple advantages over traditional inactivated influenza vaccines to be used in a pandemic, because they can be ...


    pub_type: 杂志文章


    authors: Jiang WM,Wang SC,Liu HL,Yu JM,Du X,Hou GY,Li JP,Liu S,Wang KC,Zhuang QY,Liu XM,Chen JM

    更新日期:2014-11-12 00:00:00

  • Recombinant H7 hemagglutinin forms subviral particles that protect mice and ferrets from challenge with H7N9 influenza virus.

    abstract::A novel avian-origin influenza A H7N9 virus emerged in China in 2013 and continues to cause sporadic human infections with mortality rates approaching 35%. Currently there are no approved human vaccines for H7N9 virus. Recombinant approaches including hemagglutinin (HA) and virus-like particles (VLPs) have resulted in...


    pub_type: 杂志文章


    authors: Pushko P,Pujanauski LM,Sun X,Pearce M,Hidajat R,Kort T,Schwartzman LM,Tretyakova I,Chunqing L,Taubenberger JK,Tumpey TM

    更新日期:2015-09-11 00:00:00

  • Intranasal immunization with recombinant gD2 reduces disease severity and mortality following genital challenge with herpes simplex virus type 2 in guinea pigs.

    abstract::The ability of a genetically detoxified mutant of heat labile enterotoxin (LTK63) to act as a mucosal adjuvant following intranasal immunization with recombinant gD2 has previously been reported in mice [Ugozzoli M, O'Hagan DT, Ott GS. Intranasal immunization of mice with herpes simplex virus type 2 recombinant gD2: t...


    pub_type: 杂志文章


    authors: O'Hagan D,Goldbeck C,Ugozzoli M,Ott G,Burke RL

    更新日期:1999-05-04 00:00:00

  • A fish specific expression vector containing the interferon regulatory factor 1A (IRF1A) promoter for genetic immunization of fish.

    abstract::DNA plasmid vectors were constructed with inducible rainbow trout promoters to take the place of the cytomegalovirus immediate early promoter in DNA vaccines for fish. DNA fragments containing the promoter regions upstream of the rainbow trout Mx1 and interferon regulatory factor 1A (IRF1A) genes were obtained and the...


    pub_type: 杂志文章


    authors: Alonso M,Johnson M,Simon B,Leong JA

    更新日期:2003-04-02 00:00:00

  • Hyper-attenuated MTBVAC erp mutant protects against tuberculosis in mice.

    abstract::Safety of individuals at risk of immune suppression is an important concern for live vaccines. The new-generation tuberculosis vaccine candidate MTBVAC, a genetically engineered doubly attenuated Mycobacterium tuberculosis mutant with deletions in phoP and fadD26 virulence genes has demonstrated comparable safety in d...


    pub_type: 杂志文章


    authors: Solans L,Uranga S,Aguilo N,Arnal C,Gomez AB,Monzon M,Badiola JJ,Gicquel B,Martin C

    更新日期:2014-09-08 00:00:00

  • Influences on immunization rates: Vaccination coverage of mumps, measles, rubella and varicella before and after the STIKO intervention 2011 - A retrospective study.

    abstract:BACKGROUND:In September 2011, the German Standing Committee on Vaccinations (STIKO) changed their recommendation regarding the mumps-measles-rubella-varicella vaccination (MMRV). We compared the immunization rates against MMRV in Germany before and after the STIKO intervention. METHODS:We recorded the immunization sta...


    pub_type: 杂志文章


    authors: Sanftenberg L,Schrörs HJ,Schelling J

    更新日期:2016-07-25 00:00:00

  • Impact of MenBvac, an outer membrane vesicle (OMV) vaccine, on the meningococcal carriage.

    abstract::The aim of the study was to analyze the impact of MenBvac, an outer membrane vesicle (OMV) vaccine against P1.7,16 strains, on meningococcal carriage. During a B:14:P1.7,16/ST-32 outbreak in Normandy (France), children aged 1-7 years were randomly selected to participate in the study. Among the 1082 volunteers, there ...


    pub_type: 杂志文章


    authors: Delbos V,Lemée L,Bénichou J,Berthelot G,Deghmane AE,Leroy JP,Houivet E,Hong E,Taha MK,Caron F,B14 STOP study group.

    更新日期:2013-09-13 00:00:00

  • Adjuvant effect of Cliptox on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice.

    abstract::Foot and Mouth Disease (FMD) is an acute disease caused by Foot and Mouth Disease Virus (FMDV) which causes important economy losses, this is why it is necessary to obtain a vaccine that stimulates a rapid and long-lasting protective immune response. Cliptox is a mineral microparticle that in earlier studies has shown...


    pub_type: 杂志文章


    authors: Batista A,Quattrocchi V,Olivera V,Langellotti C,Pappalardo JS,Di Giacomo S,Mongini C,Portuondo D,Zamorano P

    更新日期:2010-08-31 00:00:00

  • Enhanced immune sera and vaccine: safe approach to treat scorpion envenoming.

    abstract::Irradiation of Androctonus australis hector venom using a dose of 2 kGy has successfully abolished toxicity without reducing its antigenic or immunogenic properties. Toxicity of irradiated antigen was abolished until 20 times of LD(50) of native venom. Analysis of physiopathological effects induced by native and irrad...


    pub_type: 杂志文章


    authors: Lila BA,Laraba-Djebari F

    更新日期:2011-11-08 00:00:00

  • Generation of recombinant pandemic H1N1 influenza virus with the HA cleavable by bromelain and identification of the residues influencing HA bromelain cleavage.

    abstract::The proteolytic enzyme bromelain has been traditionally used to cleave the hemagglutinin (HA) protein at the C-terminus of the HA2 region to release the HA proteins from influenza virions. The bromelain cleaved HA (BHA) has been routinely used as an antigen to generate antiserum that is essential for influenza vaccine...


    pub_type: 杂志文章


    authors: Wang W,Suguitan AL Jr,Zengel J,Chen Z,Jin H

    更新日期:2012-01-20 00:00:00

  • Burden and trends of type-specific human papillomavirus infections and related diseases in the Asia Pacific region.

    abstract::We present the burden of human papillomavirus (HPV)-related cancers (cancers of the cervix, anogenital areas, and oral cavity and pharynx) in terms of incidence and mortality, for the countries of the Asia Pacific region. The region contains more than half of the world population, and manifests a wide geographic diver...


    pub_type: 杂志文章


    authors: Parkin DM,Louie KS,Clifford G

    更新日期:2008-08-19 00:00:00

  • Modelling the epidemiological impact of rotavirus vaccination in Germany--a Bayesian approach.

    abstract:BACKGROUND:Rotavirus (RV) infection is the primary cause of severe gastroenteritis in children aged <5 years in Germany and worldwide. In 2013 the German Standing Committee on Vaccination (STIKO) developed a national recommendation for routine RV-immunization of infants. To support informed decision-making we predicted...


    pub_type: 杂志文章


    authors: Weidemann F,Dehnert M,Koch J,Wichmann O,Höhle M

    更新日期:2014-09-08 00:00:00

  • Identification and evaluation of an infertility-associated ZP3 epitope from the marsupial brushtail possum (Trichosurus vulpecula).

    abstract::Immunologically based fertility control vaccines against zona pellucida (ZP) proteins are being developed in New Zealand for biocontrol of the brushtail possum (Trichosurus vulpecula), an introduced Australian marsupial pest. We have shown that immunization of female possums with recombinant possum ZP3 protein (rZP3) ...


    pub_type: 杂志文章


    authors: Cui X,Duckworth JA,Molinia FC,Cowan PE

    更新日期:2010-02-10 00:00:00

  • Is varicella vaccination associated with pediatric arterial ischemic stroke? A population-based cohort study.

    abstract:BACKGROUND AND PURPOSE:Varicella disease is a risk factor for pediatric Arterial Ischemic Stroke (AIS). Isolated case reports have emerged suggesting that varicella vaccination may also pose a risk for AIS. METHODS:This retrospective population-based cohort study assessed the risk of AIS in children who received a var...


    pub_type: 杂志文章


    authors: MacDonald SE,Dover DC,Hill MD,Kirton A,Simmonds KA,Svenson LW

    更新日期:2018-05-11 00:00:00

  • Infectious bursal disease virus rescued efficiently with 3' authentic RNA sequence induces humoral immunity without bursal atrophy.

    abstract::A reverse genetics infectious bursal disease virus (RG-IBDV) that contained authentic 3' RNA sequence was characterized both in vitro and in vivo. LP1-IBDV, a cell line-adapted IBDV strain variant E (VE) was used as the parent virus for constructing viral cDNA clones. Authentic 3' RNA sequence was generated by using c...


    pub_type: 杂志文章


    authors: Mosley YY,Wu CC,Lin TL

    更新日期:2013-01-11 00:00:00

  • The changing landscape of respiratory syncytial virus.

    abstract::Recognition of the acute and chronic burden of respiratory syncytial virus (RSV) lower respiratory tract infections (LRTI) sparked a wave of initiatives to develop preventive and therapeutic products against the pathogen in recent years. RSV is a leading cause of hospitalization in infants in industrialized and develo...


    pub_type: 杂志文章,评审


    authors: Polack FP

    更新日期:2015-11-25 00:00:00

  • Use of predictive markers of HIV disease progression in vaccine trials.

    abstract::Generating broadly neutralizing antibodies with candidate vaccines has remained an elusive goal. Consequently, vaccine candidates developed have aimed at eliciting cell-mediated immune effector activities (CMI) that could delay disease progression, and maybe also limit secondary transmission, by controlling virus repl...


    pub_type: 杂志文章,评审


    authors: Gurunathan S,Habib RE,Baglyos L,Meric C,Plotkin S,Dodet B,Corey L,Tartaglia J

    更新日期:2009-03-23 00:00:00

  • Gene gun DNA immunization of cattle induces humoral and CD4 T-cell-mediated immune responses against the Theileria parva polymorphic immunodominant molecule.

    abstract::Theileria parva kills over one million cattle annually in sub-Saharan Africa. Parasite genetic complexity, cellular response immunodominance, and bovine MHC diversity have precluded traditional vaccine development. One potential solution is gene gun (GG) immunization, which enables simultaneous administration of one o...


    pub_type: 杂志文章


    authors: Fry LM,Bastos RG,Stone BC,Williams LB,Knowles DP,Murphy SC

    更新日期:2019-03-14 00:00:00

  • Immunogenicity and protective efficacy of a novel foot-and-mouth disease virus empty-capsid-like particle with improved acid stability.

    abstract::Foot-and-mouth disease virus (FMDV) is the etiological agent of a highly contagious disease that affects cloven-hoofed animal species. The FMDV capsid is highly acid labile and viral particles lose their immunogenicity when they disassemble at mildly acidic pHs. The viral capsid of FMDV serotype O is more sensitive th...


    pub_type: 杂志文章


    authors: Xie Y,Li H,Qi X,Ma Y,Yang B,Zhang S,Chang H,Yin X,Li Z

    更新日期:2019-03-28 00:00:00

  • Beta-propiolactone treatment impairs the biological activity of residual DNA from BHK-21 cells infected with rabies virus.

    abstract::The effects of beta-propiolactone (BPL), an alkylating and virus inactivating agent, on the structural and in vitro biological properties of different DNA preparations from BHK-21 cells were investigated. Both uninfected and rabies virus-infected cells were used. Purified cellular DNA (celDNA) was used as the referenc...


    pub_type: 杂志文章


    authors: Morgeaux S,Tordo N,Gontier C,Perrin P

    更新日期:1993-01-01 00:00:00

  • Detoxification of endotoxin by aluminum hydroxide adjuvant.

    abstract::Langmuir adsorption isotherms of endotoxin and aluminum-containing adjuvants at pH 7.4 and 25 degrees C revealed that aluminum hydroxide adjuvant has a greater adsorption capacity (283 microg/mg Al) and adsorption coefficient (1.3x10(4) ml/miccrog) than aluminum phosphate adjuvant (3.0 microg/mg Al, 0.20 ml/microg). T...


    pub_type: 杂志文章


    authors: Shi Y,HogenEsch H,Regnier FE,Hem SL

    更新日期:2001-02-08 00:00:00