Abstract:
:We evaluated spleen function in 193 children with sickle cell anemia 8 to 18 months of age by (99m)Tc sulfur-colloid liver-spleen scan and correlated results with clinical and laboratory parameters, including 2 splenic biomarkers: pitted cell counts (PIT) and quantitative Howell-Jolly bodies (HJB) enumerated by flow cytometry. Loss of splenic function began before 12 months of age in 86% of infants in association with lower total or fetal hemoglobin and higher white blood cell or reticulocyte counts, reinforcing the need for early diagnosis and diligent preventive care. PIT and HJB correlated well with each other and liver-spleen scan results. Previously described biomarker threshold values did define patients with abnormal splenic function, but our data suggest that normal spleen function is better predicted by PIT of ≤1.2% or HJB ≤55/10(6) red blood cells and absent function by PIT ≥4.5% or HJB ≥665/10(6). HJB is methodologically advantageous compared with PIT, but both are valid biomarkers of splenic function. This trial was registered at www.clinicaltrials.gov as #NCT00006400.
journal_name
Bloodjournal_title
Bloodauthors
Rogers ZR,Wang WC,Luo Z,Iyer RV,Shalaby-Rana E,Dertinger SD,Shulkin BL,Miller JH,Files B,Lane PA,Thompson BW,Miller ST,Ware RE,BABY HUG.doi
10.1182/blood-2010-04-278747subject
Has Abstractpub_date
2011-03-03 00:00:00pages
2614-7issue
9eissn
0006-4971issn
1528-0020pii
blood-2010-04-278747journal_volume
117pub_type
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