Abstract:
:Prothrombin activation can proceed through the intermediates meizothrombin or prethrombin-2. To assess the contributions that these 2 intermediates make to prothrombin activation in tissue factor (Tf)-activated blood, immunoassays were developed that measure the meizothrombin antithrombin (mTAT) and α-thrombin antithrombin (αTAT) complexes. We determined that Tf-activated blood produced both αTAT and mTAT. The presence of mTAT suggested that nonplatelet surfaces were contributing to approximately 35% of prothrombin activation. Corn trypsin inhibitor-treated blood was fractionated to yield red blood cells (RBCs), platelet-rich plasma (PRP), platelet-poor plasma (PPP), and buffy coat. Compared with blood, PRP reconstituted with PPP to a physiologic platelet concentration showed a 2-fold prolongation in the initiation phase and a marked decrease in the rate and extent of αTAT formation. Only the addition of RBCs to PRP was capable of normalizing αTAT generation. FACS on glycophorin A-positive cells showed that approximately 0.6% of the RBC population expresses phosphatidylserine and binds prothrombinase (FITC Xa·factor Va). These data indicate that RBCs participate in thrombin generation in Tf-activated blood, producing a membrane that supports prothrombin activation through the meizothrombin pathway.
journal_name
Bloodjournal_title
Bloodauthors
Whelihan MF,Zachary V,Orfeo T,Mann KGdoi
10.1182/blood-2012-05-427856subject
Has Abstractpub_date
2012-11-01 00:00:00pages
3837-45issue
18eissn
0006-4971issn
1528-0020pii
blood-2012-05-427856journal_volume
120pub_type
杂志文章相关文献
BLOOD文献大全abstract::Purified T lymphocytes (E rosetting cells) isolated from the involved lymphoid organs (lymph nodes and spleen) of five patients with Hodgkin's disease (HD) were cloned under culture conditions (phytohemagglutinin plus interleukin-2) that allow clonal expansion of most T lymphocytes. A total number of 104 CD4+ T cell c...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::The importance of the second messengers calcium (Ca(2+)) and diacylglycerol (DAG) in platelet signal transduction was established more than 30 years ago. Whereas protein kinase C (PKC) family members were discovered as the targets of DAG, little is known about the molecular identity of the main Ca(2+) sensor(s). We he...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-04-218768
更新日期:2009-09-17 00:00:00
abstract::Human herpesvirus 6 (HHV-6) is detected in the plasma of approximately 40% of patients undergoing hematopoietic cell transplantation (HCT) and sporadically causes encephalitis in this population. The effect of HHV-6 reactivation on central nervous system function has not been fully characterized. This prospective stud...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2010-10-316083
更新日期:2011-05-12 00:00:00
abstract::Allogeneic stem cell transplantation (SCT) is curative for hemophagocytic lymphohistiocytosis (HLH). However, patients frequently have significant morbidity before transplantation and there is high transplant-related mortality (TRM). Because first-degree HLH is caused by immune dysregulation, a reduced-intensity condi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-05-1819
更新日期:2006-02-01 00:00:00
abstract::Specific monoclonal and polyclonal antibody reagents and a double antigen indirect immunofluorescence microscopy technique were used to visualize coagulation factor V in human bone marrow. Marrow aspirates were smeared directly on glass slides, or washed and cytospun onto glass slides, or processed and plated into a p...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
abstract::The rates of endocytosis, intracellular degradation, and cell-surface shedding of 125I-labeled monoclonal antibodies (MoAbs) HD-37 (anti-CD19), B1 (anti-CD20), MB-1 (anti-CD37), BC8 (anti-CD45), and DA4-4 (anti-mu) by B-lymphoma cells were compared by cellular radioimmunoassay, ultrastructural autoradiography, sodium ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::IgE and IgE receptors (FcεRI) are well-known inducers of allergy. We recently found in mice that active systemic anaphylaxis depends on IgG and IgG receptors (FcγRIIIA and FcγRIV) expressed by neutrophils, rather than on IgE and FcεRI expressed by mast cells and basophils. In humans, neutrophils, mast cells, basophils...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-07-367334
更新日期:2012-03-15 00:00:00
abstract::Recently, we have identified scavenger receptor class A member I (SR-AI) as a receptor for coagulation factor X (FX), mediating the formation of an FX reservoir at the macrophage surface. Here, we demonstrate that the FX/SR-AI-complex comprises a third protein, pentraxin-2 (PTX2). The presence of PTX2 is essential to ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-06-724351
更新日期:2017-04-27 00:00:00
abstract::Reversible interactions of glycoconjugates on leukocytes with P- and E-selectin on endothelial cells mediate tethering and rolling of leukocytes in inflamed vascular beds, the first step in their recruitment to sites of injury. Although selectin ligands on hematopoietic precursors have been identified, here we review ...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2011-07-343566
更新日期:2011-12-22 00:00:00
abstract::Ionophore A23187-induced 14C-serotonin secretion and thromboxane B2(TxB2) formation were found to be absent in citrated platelet-rich plasma (PRP) from thrombasthenic subjects and in normal PRP treated with glycoprotein (GP) IIb-IIIa complex-specific monoclonal antibodies. Both responses were restored to normal levels...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::The reported outcome of hematopoietic stem cell transplantation (HSCT) from HLA-mismatched family members has been inconsistent. The object of this study was to evaluate the true impact of HLA-mismatch by using recent data from a homogenous population, excluding HSCT procedures that used graft manipulations, and by co...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-02-0430
更新日期:2003-08-15 00:00:00
abstract::Seventy-five patients with resistant acute leukemia or lymphoma received high-dose cyclophosphamide and etoposide to explore the activity of this combination in resistant hematologic malignancies, and to determine the maximum doses of these drugs that can be combined without bone marrow transplantation. Etoposide was ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::Custom-made monoclonal anti-idiotype antibodies (anti-Id MoAbs) have been tested as a treatment modality in 34 non-Hodgkin's lymphoma (NHL) patients. Partial or complete tumor remissions have been induced with this treatment in 68% of these patients. One mechanism by which anti-idiotype antibodies may have induced the...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-02-15 00:00:00
abstract::Human T-cell leukemia virus type-I (HTLV-I), the etiologic agent of adult T-cell leukemia (ATL) transforms human T cells both in vivo and in vitro. However, the long latency period between infection and development of ATL, as well as the small fraction of the infected population that actually develops this disease, su...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-12-15 00:00:00
abstract::Viral infections are common and are potentially life-threatening in patients with moderate to severe primary immunodeficiency disorders. Because T-cell immunity contributes to the control of many viral pathogens, adoptive immunotherapy with virus-specific T cells (VSTs) has been a logical and effective way of combatin...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood.2019000924
更新日期:2020-02-27 00:00:00
abstract::Hypomethylating agents (HMAs) improve survival in patients with higher-risk myelodysplastic syndromes (MDS) but are less well-studied in lower-risk disease. We compared the safety and efficacy of low-dose decitabine vs low-dose azacitidine in this group of patients. Adults with low- or intermediate 1-risk MDS or MDS/m...
journal_title:Blood
pub_type: 杂志文章,随机对照试验
doi:10.1182/blood-2017-06-788497
更新日期:2017-09-28 00:00:00
abstract::Bcr-Abl tyrosine kinase activity initiates a number of intracellular signaling cascades that result in leukemogenesis. Imatinib mesylate, a specific Bcr-Abl tyrosine kinase inhibitor, has been highly successful in the treatment of chronic myelogenous leukemia (CML). However, the emergence of imatinib resistance and th...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-10-3864
更新日期:2005-04-15 00:00:00
abstract::Although the mechanisms by which all-trans-retinoic acid (RA) regulates gene transcription are well understood, very little is known on the signaling events regulating RA-dependent initiation of mRNA translation. We examined whether the mammalian target of rapamycin (mTOR)/p70 S6 kinase pathway is activated by RA. RA ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-06-2078
更新日期:2005-02-15 00:00:00
abstract::Immune-mediated platelet destruction is most frequently caused by allo- or autoantibodies via Fcγ receptor-dependent phagocytosis. Disease severity can be predicted neither by antibody isotype nor by titer, indicating that other factors play a role. Here we show that the acute phase protein C-reactive protein (CRP), a...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-05-579110
更新日期:2015-03-12 00:00:00
abstract::Acute myeloid leukemia (AML) is driven by niche-derived and cell-autonomous stimuli. Although many cell-autonomous disease drivers are known, niche-dependent signaling in the context of the genetic disease heterogeneity has been difficult to investigate. Here, we analyzed the role of Bruton tyrosine kinase (BTK) in AM...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-06-585216
更新日期:2015-03-19 00:00:00
abstract::Recombinant human interleukin-2 (IL-2), administered to cancer patients by continuous intravenous (IV) infusion (3 x 10(6) U/m2/d), was found to induce the in vivo production of colony-stimulating factors (CSF). Plasma obtained from patients during IL-2 treatment stimulated in vitro colony formation of normal human bo...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-10-15 00:00:00
abstract::The present study investigates the relationship between mitochondrial activity and the expression of the BCL-2 gene in a panel of six human and murine leukemia/lymphoma cell lines. The cell lines all contained normal glucocorticoid receptors but differed widely in sensitivity to dexamethasone, ranging from very sensit...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::T-cell receptor (TCR) beta-chain proteins appear early (approximately 15th week of gestation) during human thymic ontogenesis. These beta-chain proteins, which appear before terminal deoxynucleotidyl transferase (TdT), could be an expression of a fully rearranged (V-D-J), incompletely rearranged (D-J), or germline TCR...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-03-15 00:00:00
abstract::We have shown that human B-cell non-Hodgkin lymphomas (B-NHLs) express heat shock protein (HSP)H1/105 in function of their aggressiveness. Here, we now clarify its role as a functional B-NHL target by testing the hypothesis that it promotes the stabilization of key lymphoma oncoproteins. HSPH1 silencing in 4 models of...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-07-590034
更新日期:2015-03-12 00:00:00
abstract::Hoyeraal Hreidarsson syndrome (HHS) is a form of dyskeratosis congenita (DC) characterized by bone marrow failure, intrauterine growth retardation, developmental delay, microcephaly, cerebellar hypoplasia, immunodeficiency, and extremely short telomeres. As with DC, mutations in genes encoding factors required for tel...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-08-447755
更新日期:2013-05-02 00:00:00
abstract::Hand mirror cells are a morphological configuration that are seen in immunologically stimulated lymphocytes and can be induced by antigen--antibody complexes. Therefore, the bone marrow and peripheral blood plasma of two patients with acute lymphoblastic leukemia--hand mirror variant were evaluated for the presence of...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-10-01 00:00:00
abstract::The platelet release reaction plays a critical role in thrombosis and contributes to the events that follow hemostasis. Previous studies have shown that platelet secretion is mediated by Soluble NSF Attachment Protein Receptor (SNARE) proteins from granule and plasma membranes. The SNAREs form transmembrane complexes ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-05-430603
更新日期:2012-09-20 00:00:00
abstract::HIV-1-associated thrombocytopenia (HIV-1-ITP) is a common complication of HIV-1 infection, frequently caused by increased peripheral platelet destruction mediated by antiplatelet antibodies (Abs) and/or platelet-bound immune complexes. Little is known about the specificity of the antiplatelet Abs at a molecular level....
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-01-0386
更新日期:2004-12-15 00:00:00
abstract::Integrin-β7 (ITGB7) mRNA is detected in multiple myeloma (MM) cells and its presence is correlated with MAF gene activation. Although the involvement of several integrin family members in MM-stoma cell interaction is well documented, the specific biologic functions regulated by integrin-β7 in MM are largely unknown. C...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-06-292243
更新日期:2011-06-09 00:00:00
abstract::Peripheral blood mononuclear cells (PBMC) from 17 patients receiving HLA-identical sibling bone marrow grafts were stimulated with host pretransplant PBMC. Cytotoxic T-cell lines (TCL) with specificity for host pretransplant PBMC were obtained from 9 of these patients, all presenting with severe graft-versus-host dise...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-04-15 00:00:00