Human platelet cytoskeletons: specific content of glycolipids and phospholipids.

Abstract:

:The lipid composition of platelet cytoskeletons was analyzed. Triton X-100 (0.5%) was used to prepare cytoskeletons from thrombin-treated platelets. The lipid/protein ratio of platelet cytoskeletons was 0.260 and the phospholipid/protein ratio was 0.177, which were comparable to the ratios present in platelets. However, there was a selective enrichment of platelet lipids in platelet cytoskeletons. Only 2 of the 5 major platelet phospholipids were detected. About 14% platelet sphingomyelin and 2% platelet phosphatidylcholine were present in platelet cytoskeletons. Only 1 of the 4 platelet neutral glycolipids, trihexosyl ceramide, was detected and was about 7% of that in intact platelets. Two percent of platelet hematoside, the predominant ganglioside in platelets, was found in cytoskeletons. Six percent of platelet cholesterol was present in platelet cytoskeletons, while no other neutral lipid could be detected. The study demonstrates that the lipid/protein ratio of platelet cytoskeletons is similar to that in platelets, but the composition of cytoskeleton lipids is specific and distinctly different from that in platelets. The selective glycolipid and phospholipid composition of cytoskeletons may be important for cytoskeleton and platelet function.

journal_name

Blood

journal_title

Blood

authors

Schick PK,Tuszynski GP,Vander Voort PW

subject

Has Abstract

pub_date

1983-01-01 00:00:00

pages

163-6

issue

1

eissn

0006-4971

issn

1528-0020

journal_volume

61

pub_type

杂志文章

相关文献

BLOOD文献大全
  • Activation of the contact system in insect-sting anaphylaxis: association with the development of angioedema and shock.

    abstract::A postulated role of the contact system in anaphylactic reactions to insect stings was investigated. During prospective, in-hospital sting challenge, we collected serial blood samples from five normal volunteers and 16 patients with a history of insect-sting anaphylaxis. Activation of the contact system was assessed b...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: van der Linden PW,Hack CE,Eerenberg AJ,Struyvenberg A,van der Zwan JK

    更新日期:1993-09-15 00:00:00

  • Randomized study of reduced-intensity chemotherapy combined with imatinib in adults with Ph-positive acute lymphoblastic leukemia.

    abstract::In this study, we randomly compared high doses of the tyrosine kinase inhibitor imatinib combined with reduced-intensity chemotherapy (arm A) to standard imatinib/hyperCVAD (cyclophosphamide/vincristine/doxorubicin/dexamethasone) therapy (arm B) in 268 adults (median age, 47 years) with Philadelphia chromosome-positiv...

    journal_title:Blood

    pub_type: 杂志文章,随机对照试验

    doi:10.1182/blood-2015-02-627935

    authors: Chalandon Y,Thomas X,Hayette S,Cayuela JM,Abbal C,Huguet F,Raffoux E,Leguay T,Rousselot P,Lepretre S,Escoffre-Barbe M,Maury S,Berthon C,Tavernier E,Lambert JF,Lafage-Pochitaloff M,Lhéritier V,Chevret S,Ifrah N,Dombr

    更新日期:2015-06-11 00:00:00

  • Epinephrine-induced activation of LW-mediated sickle cell adhesion and vaso-occlusion in vivo.

    abstract::Sickle red cell (SS RBC) adhesion is believed to contribute to the process of vaso-occlusion in sickle cell disease (SCD). We previously found that the LW RBC adhesion receptor can be activated by epinephrine to mediate SS RBC adhesion to endothelial alphavbeta3 integrin. To determine the contribution of LW activation...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2006-11-056101

    authors: Zennadi R,Moeller BJ,Whalen EJ,Batchvarova M,Xu K,Shan S,Delahunty M,Dewhirst MW,Telen MJ

    更新日期:2007-10-01 00:00:00

  • Inhibition of HIV-1 infection by a CCR5-binding cyclophilin from Toxoplasma gondii.

    abstract::The activation of murine dendritic cells by Toxoplasma gondii has recently been shown to depend on a parasite protein that signals through the chemokine receptor CCR5. Here we demonstrate that this molecule, cyclophilin-18 (C-18), is an inhibitor of HIV-1 cell fusion and infection with cell-free virus. T gondii C-18 e...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2003-04-1096

    authors: Golding H,Aliberti J,King LR,Manischewitz J,Andersen J,Valenzuela J,Landau NR,Sher A

    更新日期:2003-11-01 00:00:00

  • ICA-17043, a novel Gardos channel blocker, prevents sickled red blood cell dehydration in vitro and in vivo in SAD mice.

    abstract::A prominent feature of sickle cell anemia is the presence of dehydrated red blood cells (RBCs) in circulation. Loss of potassium (K(+)), chloride (Cl(-)), and water from RBCs is thought to contribute to the production of these dehydrated cells. One main route of K(+) loss in the RBC is the Gardos channel, a calcium (C...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2002-05-1433

    authors: Stocker JW,De Franceschi L,McNaughton-Smith GA,Corrocher R,Beuzard Y,Brugnara C

    更新日期:2003-03-15 00:00:00

  • ADA-deficient SCID is associated with a specific microenvironment and bone phenotype characterized by RANKL/OPG imbalance and osteoblast insufficiency.

    abstract::Adenosine deaminase (ADA) deficiency is a disorder of the purine metabolism leading to combined immunodeficiency and systemic alterations, including skeletal abnormalities. We report that ADA deficiency in mice causes a specific bone phenotype characterized by alterations of structural properties and impaired mechanic...

    journal_title:Blood

    pub_type: 杂志文章,多中心研究

    doi:10.1182/blood-2009-03-209221

    authors: Sauer AV,Mrak E,Hernandez RJ,Zacchi E,Cavani F,Casiraghi M,Grunebaum E,Roifman CM,Cervi MC,Ambrosi A,Carlucci F,Roncarolo MG,Villa A,Rubinacci A,Aiuti A

    更新日期:2009-10-08 00:00:00

  • Second cancers and late toxicities after treatment of aggressive non-Hodgkin lymphoma with the ACVBP regimen: a GELA cohort study on 2837 patients.

    abstract::The survival of patients with aggressive non-Hodgkin lymphoma (NHL) is increasing, but the incidence of secondary cancer and late toxicity is poorly defined for those treated with cyclophosphamide-hydroxydaunomycin/doxorubicin-Oncovin-prednisone (CHOP)-like chemotherapy. From February 1984 to January 1998, 2837 patien...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2003-04-1124

    authors: André M,Mounier N,Leleu X,Sonet A,Brice P,Henry-Amar M,Tilly H,Coiffier B,Bosly A,Morel P,Haioun C,Gaulard P,Reyes F,Gisselbrecht C,Groupe D'Etude Des Lymphomes De L'Adulte.

    更新日期:2004-02-15 00:00:00

  • A common polymorphism in the annexin V Kozak sequence (-1C>T) increases translation efficiency and plasma levels of annexin V, and decreases the risk of myocardial infarction in young patients.

    abstract::Annexin V has phospholipid-binding capacity and plays a potent antithrombotic role. Recently, a C to T transition has been described in the Kozak region of this gene, affecting the nucleotide preceding the initiation ATG codon. We have developed a simple method to detect this genetic change, showing by analysis of 580...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: González-Conejero R,Corral J,Roldán V,Martínez C,Marín F,Rivera J,Iniesta JA,Lozano ML,Marco P,Vicente V

    更新日期:2002-09-15 00:00:00

  • FLT3 activation cooperates with MLL-AF4 fusion protein to abrogate the hematopoietic specification of human ESCs.

    abstract::Mixed-lineage leukemia (MLL)-AF4 fusion arises prenatally in high-risk infant acute pro-B-lymphoblastic leukemia (pro-B-ALL). In human embryonic stem cells (hESCs), MLL-AF4 skewed hematoendothelial specification but was insufficient for transformation, suggesting that additional oncogenic insults seem required for MLL...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2012-11-470146

    authors: Bueno C,Ayllón V,Montes R,Navarro-Montero O,Ramos-Mejia V,Real PJ,Romero-Moya D,Araúzo-Bravo MJ,Menendez P

    更新日期:2013-05-09 00:00:00

  • Platelet adhesion involves a novel interaction between vimentin and von Willebrand factor under high shear stress.

    abstract::The interaction between platelet receptor glycoprotein Ibα and the A1 domain of von Willebrand factor (VWF) mediates tethering/translocation of platelets to sites of vascular injury. Unexpectedly, we observed platelets translocating over A1A2A3 domains protein slower than on A1 domain at high shear stress. This observ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2013-10-530428

    authors: Da Q,Behymer M,Correa JI,Vijayan KV,Cruz MA

    更新日期:2014-04-24 00:00:00

  • Relationship of the human erythrocyte Wrb antigen to an interaction between glycophorin A and band 3.

    abstract::The Wrb antigen is a high-frequency human erythrocyte antigen invariably absent from En (a-) erythrocytes, which lack glycophorin A. However, glycophorin A from En (a+) Wr (a+b-) red cells has an amino acid sequence identical to that of glycophorin A from Wr (b+) erythrocytes. Evidence has suggested that the Wrb antig...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Telen MJ,Chasis JA

    更新日期:1990-08-15 00:00:00

  • Erythrocyte vitamin E is oxidized at a lower peroxide concentration in neonates than in adults.

    abstract::Erythrocytes of neonates and adults were incubated with increasing concentrations of H2O2 in the presence of a catalase inhibitor and in the absence of glucose; the pattern of oxidation of vitamin E was analyzed in relationship to that of glutathione, hemoglobin, and polyunsaturated fatty acids (PUFA), and in relation...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Vanderpas J,Vertongen F

    更新日期:1985-12-01 00:00:00

  • New insights into the mechanisms of hematopoietic cell transformation by activated receptor tyrosine kinases.

    abstract::A large number of alterations in genes encoding receptor tyrosine kinase (RTK), namely FLT3, c-KIT, platelet-derived growth factor (PDGF) receptors, fibroblast growth factor (FGF) receptors, and the anaplastic large cell lymphoma kinase (ALK), have been found in hematopoietic malignancies. They have drawn much attenti...

    journal_title:Blood

    pub_type: 杂志文章,评审

    doi:10.1182/blood-2010-04-279752

    authors: Toffalini F,Demoulin JB

    更新日期:2010-10-07 00:00:00

  • Myeloma cells upregulate interleukin-6 secretion in osteoblastic cells through cell-to-cell contact but downregulate osteocalcin.

    abstract::Previous studies have shown that bone marrow, especially the bone microenvironment, may play an important role in the pathogenesis of multiple myeloma (MM). To elucidate the relationship between myeloma cells and bone cells, mainly osteoblasts, we have established a coculture system between two interleukin-6 (IL-6)-de...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Barillé S,Collette M,Bataille R,Amiot M

    更新日期:1995-10-15 00:00:00

  • A crucial role for the homeodomain transcription factor Hhex in lymphopoiesis.

    abstract::The hematopoietically expressed homeobox gene, Hhex, is a transcription factor that is important for development of definitive hematopoietic stem cells (HSCs) and B cells, and that causes T-cell leukemia when overexpressed. Here, we have used an Hhex inducible knockout mouse model to study the role of Hhex in adult he...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2014-06-579813

    authors: Jackson JT,Nasa C,Shi W,Huntington ND,Bogue CW,Alexander WS,McCormack MP

    更新日期:2015-01-29 00:00:00

  • Reduction of the inhibitory antibody response to human factor VIII in hemophilia A mice by mutagenesis of the A2 domain B-cell epitope.

    abstract::Approximately 25% of patients with hemophilia A develop inhibitory antibodies after treatment with factor VIII. Most of the inhibitory activity is directed against epitopes in the A2 and C2 domains. Anti-A2 inhibitory antibodies recognize a 25-residue segment bounded by R484-I508. Several antigenic residues in this se...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2003-11-3891

    authors: Parker ET,Healey JF,Barrow RT,Craddock HN,Lollar P

    更新日期:2004-08-01 00:00:00

  • Soluble HLA-G dampens CD94/NKG2A expression and function and differentially modulates chemotaxis and cytokine and chemokine secretion in CD56bright and CD56dim NK cells.

    abstract::Soluble HLA-G (sHLA-G) inhibits natural killer (NK) cell functions. Here, we investigated sHLA-G-mediated modulation of (1) chemokine receptor and NK receptor expression and function and (2) cytokine and chemokine secretion in CD56bright and CD56dim NK cells. sHLA-G-treated or untreated peripheral blood (PB) and tonsi...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2011-05-352393

    authors: Morandi F,Ferretti E,Castriconi R,Dondero A,Petretto A,Bottino C,Pistoia V

    更新日期:2011-11-24 00:00:00

  • 2-Chlorodeoxyadenosine: an active agent in the treatment of cutaneous T-cell lymphoma.

    abstract::Cutaneous T-cell lymphomas are disfiguring malignant lymphoproliferative disorders for which standard therapy has been principally palliative. 2-Chlorodeoxyadenosine (2-CdA), a new purine analogue resistant to degradation by adenosine deaminase that has substantial activity against lymphoid neoplasms, was administered...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Saven A,Carrera CJ,Carson DA,Beutler E,Piro LD

    更新日期:1992-08-01 00:00:00

  • Use of 8-methoxypsoralen and ultraviolet-A pretreated platelet concentrates to prevent alloimmunization against class I major histocompatibility antigens.

    abstract::The use of 8-methoxypsoralen (8-MOP) and UV-A irradiation to inactivate contaminating donor leukocytes in platelet concentrates and to prevent primary alloimmunization against donor class I major histocompatibility (MHC) antigens in mice was investigated. CBA/CaH-T6J mice with the H2k haplotype and BALB/cByJ mice with...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Grana NH,Kao KJ

    更新日期:1991-06-01 00:00:00

  • Clinical relevance of mutations and gene-expression changes in adult acute myeloid leukemia with normal cytogenetics: are we ready for a prognostically prioritized molecular classification?

    abstract::Recent molecular analyses of leukemic blasts from pretreatment marrow or blood of patients with acute myeloid leukemia (AML) and a normal karyotype, the largest cytogenetic subset (ie, 40%-49%) of AML, have revealed a striking heterogeneity with regard to the presence of acquired gene mutations and changes in gene exp...

    journal_title:Blood

    pub_type: 杂志文章,评审

    doi:10.1182/blood-2006-06-001149

    authors: Mrózek K,Marcucci G,Paschka P,Whitman SP,Bloomfield CD

    更新日期:2007-01-15 00:00:00

  • A study of the kinetics of ADP-triggered platelet shape change.

    abstract::The rapid transformation of human blood platelets from inert discoid cells to spheroechinocytes that is induced by adenosine diphosphate (ADP) has been followed by right-angle light scattering intensity measurements using a laser light source and a sensitive photomultiplier. Two steps have been observed, and their rat...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Hantgan RR

    更新日期:1984-10-01 00:00:00

  • Germline CDKN2A mutation implicated in predisposition to multiple myeloma.

    abstract::Germline mutations of the CDKN2A (p16(INK4A)) tumor suppressor gene predispose patients to melanoma and pancreatic carcinoma. In contrast, mutations of the murine CDKN2A gene predispose BALB/c mice to pristane-induced plasmacytoma. We describe here a family in which a germline mutation of CDKN2A is present in 4 indivi...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Dilworth D,Liu L,Stewart AK,Berenson JR,Lassam N,Hogg D

    更新日期:2000-03-01 00:00:00

  • Murine hematopoietic stem and progenitor cells: I. Enrichment and biologic characterization.

    abstract::Murine bone marrow cells were fractionated by fluorescence-activated cell sorting into Rh123lo Lin- c-kit+ Ly6A+, Rh123hi Lin-c-kit+ Ly6A+, and Lin- c-kit+ Ly6A- populations within which most, if not all, of the hematopoietic activities of the marrow resided. The Rh123lo Lin- c-kit+Ly6A+ cells, which consist exclusive...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Li CL,Johnson GR

    更新日期:1995-03-15 00:00:00

  • Monitoring "mini-intensity" anticoagulation with warfarin: comparison of the prothrombin time using a sensitive thromboplastin with prothrombin fragment F1+2 levels.

    abstract::Treatment with warfarin using a target International Normalized Ratio (INR) range of 1.7 to 2.5 is efficacious for many clinical indications, but the minimal intensity of anticoagulation required for antithrombotic protection has yet to be determined. To evaluate whether patients could be reliably monitored with a les...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Millenson MM,Bauer KA,Kistler JP,Barzegar S,Tulin L,Rosenberg RD

    更新日期:1992-04-15 00:00:00

  • AU-rich RNA binding proteins in hematopoiesis and leukemogenesis.

    abstract::Posttranscriptional mechanisms are now widely acknowledged to play a central role in orchestrating gene-regulatory networks in hematopoietic cell growth, differentiation, and tumorigenesis. Although much attention has focused on microRNAs as regulators of mRNA stability/translation, recent data have highlighted the ro...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2011-07-347237

    authors: Baou M,Norton JD,Murphy JJ

    更新日期:2011-11-24 00:00:00

  • A monoclonal antibody that recognizes a neo-antigen exposed in the E domain of fibrin monomer complexed with fibrinogen or its derivatives: its application to the measurement of soluble fibrin in plasma.

    abstract::Using urea-solubilized human fibrin monomer as an immunogen, we raised in mice a battery of monoclonal antibodies that reacted with the immunogen but not with urea-treated or native fibrinogen. Although they all failed to react with acid-solubilized fibrin monomer (acid-FM) alone, an antibody designated as IF-43 was f...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Soe G,Kohno I,Inuzuka K,Itoh Y,Matsuda M

    更新日期:1996-09-15 00:00:00

  • The active monomeric form of macrophage inflammatory protein-1 alpha interacts with high- and low-affinity classes of receptors on human hematopoietic cells.

    abstract::Macrophage inflammatory protein-1 alpha (MIP-1 alpha) and its human homologue GOS19.1/LD78 are members of the C-C chemokine/intercrine family of secreted proteins. They have proinflammatory properties and also inhibit cell cycle progression of hematopoietic stem cells. Characterization of MIP-1 alpha receptor(s) has b...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Avalos BR,Bartynski KJ,Elder PJ,Kotur MS,Burton WG,Wilkie NM

    更新日期:1994-09-15 00:00:00

  • Role for ADAP in shear flow-induced platelet mechanotransduction.

    abstract::Binding of platelets to fibrinogen via integrin alphaIIbbeta3 stimulates cytoskeletal reorganization and spreading. These responses depend on tyrosine phosphorylation of multiple proteins by Src family members and Syk. Among Src substrates in platelets is adhesion- and degranulation-promoting adapter protein (ADAP), a...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2009-08-238238

    authors: Kasirer-Friede A,Ruggeri ZM,Shattil SJ

    更新日期:2010-03-18 00:00:00

  • Bleomycin in older early-stage favorable Hodgkin lymphoma patients: analysis of the German Hodgkin Study Group (GHSG) HD10 and HD13 trials.

    abstract::Doxorubicin, bleomycin, vinblastine sulfate, and dacarbazine (ABVD) is associated with severe toxicity in older patients, particularly from bleomycin-induced lung toxicity (BLT). Therefore, using bleomycin has been questioned in older Hodgkin lymphoma (HL) patients, especially in early-stage HL. We therefore analyzed ...

    journal_title:Blood

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1182/blood-2015-11-681064

    authors: Böll B,Goergen H,Behringer K,Bröckelmann PJ,Hitz F,Kerkhoff A,Greil R,von Tresckow B,Eichenauer DA,Bürkle C,Borchmann S,Fuchs M,Diehl V,Engert A,Borchmann P

    更新日期:2016-05-05 00:00:00

  • Plasma glucosylceramide deficiency as potential risk factor for venous thrombosis and modulator of anticoagulant protein C pathway.

    abstract::To assess the relationship between venous thrombosis and plasma glucosylceramide (GlcCer) or phosphatidylethanolamine (PE), plasma levels of GlcCer and PE were determined for 70 venous thrombosis patients referred for evaluation and 70 healthy blood donors. The mean GlcCer level, but not the PE level, was lower in pat...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood.v97.7.1907

    authors: Deguchi H,Fernández JA,Pabinger I,Heit JA,Griffin JH

    更新日期:2001-04-01 00:00:00