当前位置： SCI文献检索 > AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY期刊下所有文献 > Multiplexed analysis of circulating cell-free fetal nucleic acids for noninvasive prenatal diagnostic RHD testing.

Multiplexed analysis of circulating cell-free fetal nucleic acids for noninvasive prenatal diagnostic RHD testing.

Abstract：

OBJECTIVE:The objective of the study was the evaluation of a novel multiplex assay to detect fetal Rh blood group D-antigen gene (RHD) loci in maternal plasma from RhD-negative, pregnant women. STUDY DESIGN:An RHD genotyping assay was designed to detect exons 4, 5, 7, and 10 and RHDΨ (pseudogene) of the RHD gene along with a Y chromosome-specific assay and a generic polymerase chain reaction amplification control. Plasma samples from 150 RhD-negative pregnant women were assayed for fetal RHD genotype using the MassARRAY system. RESULTS:The fetal RHD status of 148 of 150 samples (98.7%) was correctly classified; 86 (57.3%) and 62 (41.3%) were positive and negative, respectively. CONCLUSION:This study demonstrates that noninvasive prenatal diagnostics with a single-reaction multiplexed assay is a viable path toward routine characterization of fetal RHD genotypes using circulating cell-free fetal DNA in maternal plasma on the MassARRAY system and is perhaps preferable to serologic testing as currently used clinically.

journal_name

Am J Obstet Gynecol

journal_title

American journal of obstetrics and gynecology

authors

Tynan JA,Angkachatchai V,Ehrich M,Paladino T,van den Boom D,Oeth P

doi

10.1016/j.ajog.2010.09.028

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

251.e1-6

issue

3

eissn

0002-9378

issn

1097-6868

pii

S0002-9378(10)01175-0

journal_volume

204

pub_type

杂志文章

相关文献

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY文献大全
  • Neural tube defects and elevated homocysteine levels in amniotic fluid.

    abstract：OBJECTIVE:Our purpose was to study maternal blood and amniotic fluid concentrations of homocysteine and relevant vitamins in relation to neural tube defects. STUDY DESIGN:Concentrations of total homocysteine, folate, and vitamins B12 and B6 were measured in maternal blood and amniotic fluid of 27 women carrying a fetu...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章

    doi：10.1016/0002-9378(95)90474-3

    authors： Steegers-Theunissen RP,Boers GH,Blom HJ,Nijhuis JG,Thomas CM,Borm GF,Eskes TK

    更新日期：1995-05-01 00:00:00

  • The cervical polyp: a new diagnostic and therapeutic approach with CO2 hysteroscopy.

    abstract：:CO2 hysteroscopy for diagnosis of cervical polyps was performed in 36 patients. The polyps could be located under direct vision and their attachments were found to be deeper than what is seen in regular inspection. A relatively high number of endometrial polyps were found. It is advised that the polyps be surgically r...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(78)90324-1

    authors： David A,Mettler L,Semm K

    更新日期：1978-03-15 00:00:00

  • Parturitional factors associated with membrane stripping.

    abstract：OBJECTIVE:Our purpose was to determine what factors occurring after digital separation of the chorionic membranes from the lower uterine segment (membrane stripping) are involved in observed clinical changes compared with patients not so treated. STUDY DESIGN:Thirty patients were randomly divided among a study populat...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0002-9378(93)90134-5

    authors： McColgin SW,Bennett WA,Roach H,Cowan BD,Martin JN Jr,Morrison JC

    更新日期：1993-07-01 00:00:00

  • Cardiovascular collapse during laparoscopy: a report of two cases.

    abstract：:Cardiovascular collapse is a rare but serious complication of laparoscopy. Presented here are two cases of sudden cardiovascular collapse during laparoscopy. Possible causes include severe atypical vagal reactions and carbon dioxide embolism. Arterial blood gases indicated pulmonary vascular shunting. ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(88)80044-9

    authors： Brantley JC 3rd,Riley PM

    更新日期：1988-09-01 00:00:00

  • Further evidence for the role of prolactin on human fetoplacental osmoregulation.

    abstract：:The addition of ovine prolactin (oPRL) to the fetal side of human term amnion in vitro is associated with a decrease in membrane permeability to tritiated water (THO). As the concentration of oPRL is increased from 2.5 to 20.0 micrograms per milliliter, permeability is progressively impaired. The addition of a specifi...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：

    authors： Leontic EA,Schruefer JJ,Andreassen B,Pinto H,Tyson JE

    更新日期：1979-02-15 00:00:00

  • The accuracy of clinical findings and laparoscopy in pelvic inflammatory disease.

    abstract：:The accuracy of clinical diagnosis for pelvic inflammatory disease was determined in 95 women who presented with pelvic pain to primary care physicians and then were referred to gynecologists. Laparoscopy or laparotomy with endometrial biopsy and fimbrial minibiopsy revealed that prevalence of pelvic inflammatory was ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(91)90639-9

    authors： Sellors J,Mahony J,Goldsmith C,Rath D,Mander R,Hunter B,Taylor C,Groves D,Richardson H,Chernesky M

    更新日期：1991-01-01 00:00:00

  • Conversion of Society for Maternal-Fetal Medicine abstract presentations to manuscript publications.

    abstract：OBJECTIVE:We sought to evaluate the rate of conversion of Society for Maternal-Fetal Medicine (SMFM) annual meeting abstract presentations to full manuscript publications over time. STUDY DESIGN:Full manuscript publications corresponding to all SMFM oral abstracts 2003 through 2010 inclusive, and SMFM poster abstracts...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2015.05.020

    authors： Manuck TA,Barbour K,Janicki L,Blackwell SC,Berghella V

    更新日期：2015-09-01 00:00:00

  • Comparing the effects of physical practice and mental imagery rehearsal on learning basic surgical skills by medical students.

    abstract：OBJECTIVE:The purpose of this study was to test the effects of varying the amount of physical practice and mental imagery rehearsal on learning basic surgical procedures. STUDY DESIGN:Using a sample of 65 second-year medical students, 3 randomized groups received either: (1) 3 sessions of physical practice on suturing...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/j.ajog.2004.07.075

    authors： Sanders CW,Sadoski M,Bramson R,Wiprud R,Van Walsum K

    更新日期：2004-11-01 00:00:00

  • A randomized clinical trial comparing cervical dysplasia treatment with cryotherapy vs loop electrosurgical excision procedure in HIV-seropositive women from Johannesburg, South Africa.

    abstract：BACKGROUND:Mortality associated with cervical cancer is a public health concern for women, particularly in HIV-seropositive women in resource-limited countries. HIV-seropositive women are at a higher risk of high-grade cervical precancer, which can eventually progress to invasive carcinoma as compared to HIV-seronegati...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.ajog.2017.03.022

    authors： Smith JS,Sanusi B,Swarts A,Faesen M,Levin S,Goeieman B,Ramotshela S,Rakhombe N,Williamson AL,Michelow P,Omar T,Hudgens MG,Firnhaber C

    更新日期：2017-08-01 00:00:00

  • Twin-to-twin transfusion syndrome: a subset of the twin oligohydramnios-polyhydramnios sequence.

    abstract：OBJECTIVE:Our purpose was to assess the adequacy of currently accepted criteria for the diagnosis of twin-to-twin transfusion syndrome. STUDY DESIGN:Between April 1991 and February 1992 nine consecutive women with twin gestations and ultrasonographic findings of marked growth discordance, coexistent oligohydramnios-po...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(93)90028-h

    authors： Bruner JP,Rosemond RL

    更新日期：1993-10-01 00:00:00

  • Steroid compounds and the dilatation of ovarian and uterine veins in the mouse.

    abstract：:The possible role of reproductive steroids in the vascular changes o f the estrous and menstrual cycles and of pregnancy has prompted both clinical observations and animal experiments on the influence of sex hormones on the physiology of blood vessels. Research was carried out to develop a method for exposing blood ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(72)90637-0

    authors： Forbes TR,Glassen G

    更新日期：1972-07-01 00:00:00

  • The development of an index of high-risk pregnancy.

    abstract：:Presented is a scheme by which an index of high-risk pregnancy can be evaluated. The proposed scheme is applied to a risk index developed for the purpose of illustration. The usefulness of risk indices (or any other predictive measure) can be judged in clear statistical terms. The illustrative model developed here beh...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(82)90537-3

    authors： Fortney JA,Whitehorne EW

    更新日期：1982-07-01 00:00:00

  • Surgical management of prolapse of the anterior vaginal segment: an analysis of support defects, operative morbidity, and anatomic outcome.

    abstract：OBJECTIVES:The objectives of this study were to describe a group of women with prolapse of the anterior vaginal segment associated with bilateral paravaginal defects, to report the morbidity associated with the operative repair, and to analyze the results of preoperative and postoperative pelvic support defects in five...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(94)90384-0

    authors： Shull BL,Benn SJ,Kuehl TJ

    更新日期：1994-12-01 00:00:00

  • Cigarette smoking, use of oral contraceptives, and myocardial infarction.

    abstract：:The excess risk of nonfatal myocardial infarction among users of oral contraceptives observed in England and Wales can be explained in terms of the high proportion of smokers in the study population. Among nonsmokers, the relative risk associated with the use of oral contraceptives is estimated to be 2 to 1, which is ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(76)90539-1

    authors： Jain AK

    更新日期：1976-10-01 00:00:00

  • Urethral circular smooth muscle in young and old women.

    abstract：OBJECTIVE:The purpose of this study was to determine whether thickness, fiber count, and density of urethral circular smooth muscle (CSM) differ in young and old women. STUDY DESIGN:Mid urethral hemiaxial sections from female cadavers aged 20-39 years (n = 12) and 70-89 years (n = 16) were stained for smooth muscle al...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2008.03.009

    authors： Clobes A,DeLancey JO,Morgan DM

    更新日期：2008-05-01 00:00:00

  • Placental growth factor predicts time to delivery in women with signs or symptoms of early preterm preeclampsia: a prospective multicenter study.

    abstract：BACKGROUND:There is a robust association between altered angiogenic factor concentrations, which includes placental growth factor and clinically recognized preeclampsia. Alterations in concentrations of angiogenic factors precede the clinical onset of preeclampsia by several weeks. The temporal relationship between the...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.ajog.2019.09.003

    authors： Barton JR,Woelkers DA,Newman RB,Combs CA,How HY,Boggess KA,Martin JN Jr,Kupfer K,Sibai BM,PETRA (Preeclampsia Triage by Rapid Assay) Trial.

    更新日期：2020-03-01 00:00:00

  • Can cervical cancer be prevented by a see, screen, and treat program? A pilot study.

    abstract：OBJECTIVE:Our purpose was to determine the feasibility of providing a cervical screening facility to the underprivileged communities through an educational program and a mobile clinic in which cytologic smears could be taken, screened immediately, and, when appropriate, the patients treated on site with minimal delay. ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(96)70327-7

    authors： Megevand E,Van Wyk W,Knight B,Bloch B

    更新日期：1996-03-01 00:00:00

  • Carcinoma of vagina 10 or more years following pelvic irradiation therapy.

    abstract：:Gynecologic cancer records of 4,238 patients treated between 1956 and 1974 were reviewed. Sixteen patients developed noeplasia in the cervix or vagina 10 or more years following pelvic irradiation. Three patients had squamous carcinoma in situ; the other 13 patients had invasive squamous cancer involving the upper vag...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(77)90445-8

    authors： Pride GL,Buchler DA

    更新日期：1977-03-01 00:00:00

  • Lower doses of human menopausal gonadotropin are associated with improved success with in vitro fertilization in women with low body weight.

    abstract：:Since previous data suggested hyperresponsiveness to human menopausal gonadotropin by women with low body weight, the daily dose was reduced to 1.5 ampules in patients undergoing in vitro fertilization weighing less than 55 kg. This allowed the extension of treatment to a minimum of 6 days. These changes were associat...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(88)90778-8

    authors： Halme J,Hammond MG,Bailey L,Talbert LM

    更新日期：1988-01-01 00:00:00

  • The effect of low-dose aspirin on pregnancies complicated by elevated human chorionic gonadotropin levels.

    abstract：OBJECTIVE:Our purpose was to determine whether elevated second-trimester human chorionic gonadotropin levels identify women likely to benefit from low-dose aspirin therapy. STUDY DESIGN:We evaluated second-trimester human chorionic gonadotropin levels obtained from healthy nulliparous women before screening for partic...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0002-9378(95)91373-4

    authors： Wenstrom KD,Hauth JC,Goldenberg RL,DuBard MB,Lea C

    更新日期：1995-10-01 00:00:00

  • Interrelationship between glomerular filtration rate and renal transport of sodium and chloride during fetal life.

    abstract：:The maturation of the glomerular filtration rate (GFR) and the interrelationship between the GFR and the renal transport of sodium and chloride have been evaluated during the last trimester of gestation with the use of long-term experiments in fetal lambs. An increase in GFR (milliliters per minute) parallel to the in...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(77)90712-8

    authors： Robillard JE,Sessions C,Kennedey RL,Hamel-Robillard L,Smith FG Jr

    更新日期：1977-08-01 00:00:00

  • Comparison of pregnancy dating by last menstrual period, ultrasound scanning, and their combination.

    abstract：OBJECTIVE:The purpose of this study was to evaluate the accuracy of algorithms for the assignment of gestational age with the use of the last menstrual period and early ultrasound information. STUDY DESIGN:Gestational age estimates that are based on last menstrual period, ultrasound scanning, or their combination were...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1067/mob.2002.127601

    authors： Savitz DA,Terry JW Jr,Dole N,Thorp JM Jr,Siega-Riz AM,Herring AH

    更新日期：2002-12-01 00:00:00

  • Group B streptococci during pregnancy: a comparison of two screening and treatment protocols.

    abstract：OBJECTIVE:The objective was to evaluate whether the rate of neonatal group B streptococcal infection could be reduced by screening for group B streptococci during the third trimester of pregnancy. STUDY DESIGN:Two periods in which different screening and treatment protocols were applied were compared. In period A all ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(98)70063-8

    authors： Hafner E,Sterniste W,Rosen A,Schuchter K,Plattner M,Asboth F,Philipp K

    更新日期：1998-09-01 00:00:00

  • Clinical experience with the copper-7 intrauterine contraceptive device. A preliminary report.

    abstract：:There have been 203 copper-7 IUD insertions performed in 199 women. The data from 2,230 woman-months of use have been analyzed and compared with other published reports. The copper-7 IUD was an acceptable contraceptive method in regard to effectiveness, continuation, and lack of serious complications. ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(76)90756-0

    authors： Nebel WA,Currie JL,Lassiter RE

    更新日期：1976-11-01 00:00:00

  • Preemptive analgesia for postoperative hysterectomy pain control: systematic review and clinical practice guidelines.

    abstract：OBJECTIVE:The objective of the study was to investigate the effectiveness of preemptive analgesia at pain control in women undergoing total abdominal hysterectomy. DATA SOURCES:Eligible studies, published through May 31, 2016, were retrieved through Medline, Cochrane Central Register for Controlled Trials, and Cochran...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ajog.2017.03.013

    authors： Steinberg AC,Schimpf MO,White AB,Mathews C,Ellington DR,Jeppson P,Crisp C,Aschkenazi SO,Mamik MM,Balk EM,Murphy M

    更新日期：2017-09-01 00:00:00

  • Fetal endocrine and renal responses to in utero ventilation and umbilical cord occlusion.

    abstract：OBJECTIVE:Fetal to neonatal transition involves a myriad of endocrine and renal adaptive changes triggered by multiple simultaneous stimuli. We examined the extent to which ventilation and umbilical cord occlusion have an impact on the many endocrine and renal function changes in fetal sheep at 133 +/- 1 day of gestati...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(93)90422-f

    authors： Ogundipe OA,Kullama LK,Stein H,Nijland MJ,Ervin MG,Padbury J,Ross MG

    更新日期：1993-12-01 00:00:00

  • Maternal and paternal race/ethnicity are both associated with gestational diabetes.

    abstract：OBJECTIVE:The objective of the study was to examine the rates of gestational diabetes mellitus (GDM) associated with both maternal and paternal race/ethnicity. STUDY DESIGN:This was a retrospective cohort study of all women delivered within a managed care network. Rates of GDM were calculated for maternal, paternal, a...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2010.01.082

    authors： Caughey AB,Cheng YW,Stotland NE,Washington AE,Escobar GJ

    更新日期：2010-06-01 00:00:00

  • Transvaginal electrical stimulation for female urinary incontinence.

    abstract：OBJECTIVE:Our purpose was to determine the objective and subjective efficacy of transvaginal electrical stimulation for treatment of common forms of urinary incontinence in women. STUDY DESIGN:A prospective, double-blind, randomized clinical trial included 121 women with either urinary incontinence caused by detrusor ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0002-9378(97)70142-x

    authors： Brubaker L,Benson JT,Bent A,Clark A,Shott S

    更新日期：1997-09-01 00:00:00

  • Human fibroblasts produce "pregnancy-specific" beta-1 glycoprotein in vitro.

    abstract：:Normal and genetic variant strains of nonmalignant human fibroblasts produce in vitro a substance indistinguishable from the recently described "pregnancy-specific" beta-1 glycoprotein (PSbetaG, PAPP-C, SP1). Demonstration of this presumed placenta-specific material in a nonplacental site raises questions about its no...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(79)90938-4

    authors： Rosen SW,Kaminska J,Calvert IS,Aaronson SA

    更新日期：1979-08-01 00:00:00

  • The maternal-fetal transfer of lamivudine in the ex vivo human placenta.

    abstract：OBJECTIVE:Our purpose was to measure the transfer of lamivudine ([-]-2'-deoxy-3'-thiacytidine) across the human placenta both alone and in the presence of zidovudine. STUDY DESIGN:Nine placentas from term, elective cesarean deliveries were analyzed with use of the ex vivo single cotyledon perfusion system. Antipyrine ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(97)70487-3

    authors： Bloom SL,Dias KM,Bawdon RE,Gilstrap LC 3rd

    更新日期：1997-02-01 00:00:00