Abstract：
:Enterotoxemia, a disease that affects domestic ruminants, is caused mainly by the epsilon toxin from Clostridium perfringens type D. Its eradication is virtually impossible, control and prophylaxis are based on systematic vaccination of herds with epsilon toxoids that are efficient in inducing protective antibody production. The use of recombinant toxins is one of the most promising of these strategies. This work evaluates the potency of a Cl. perfringens type D epsilon toxoid expressed by Escherichia coli administered to goats, sheep, and cattle. The etx gene was cloned into the pET-11a plasmid of E. coli strain BL21 to produce the recombinant toxin. Rabbits (n=8), goats, sheep, and cattle (n=5 for each species) were immunized with 0.2mg of the insoluble recombinant protein fraction to evaluate vaccine potency of the epsilon toxoid studied. Antibody titers were 40, 14.3, 26, and 13.1 IU/mL in the rabbit, goat, sheep, and cattle serum pools, respectively. The epsilon toxoid produced and tested in this work is adequate for immunization of ruminants against enterotoxemia.
journal_name
Vaccinejournal_title
Vaccineauthors
Lobato FC,Lima CG,Assis RA,Pires PS,Silva RO,Salvarani FM,Carmo AO,Contigli C,Kalapothakis Edoi
10.1016/j.vaccine.2010.07.046subject
Has Abstractpub_date
2010-08-31 00:00:00pages
6125-7issue
38eissn
0264-410Xissn
1873-2518pii
S0264-410X(10)01041-8journal_volume
28pub_type
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