Abstract：
:The blood-brain barrier consists of the cerebral microvascular endothelium, pericytes, astrocytes, and neurons. In this study, we analyzed the influence of primary porcine brain capillary pericytes on the barrier integrity of primary porcine brain capillary endothelial cells in a species-consistent in vitro coculture model. We were able to show a barrier integrity-decreasing impact of pericytes by transendothelial electrical resistance (TEER) and (14)C-sucrose permeability measurements. The morphology analysis revealed serrated cell borders and a shift of the endothelial morphology towards a cobblestone shape under the influence of pericytes. The analysis of the two major barrier integrity modulators vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs) displayed higher MMP activity and higher levels VEGF, MMP-2, and MMP-9 in the coculture, whereas VEGF levels were decreased by the MMP inhibitor GM6001, indicating a complex interplay of both. Inhibition experiments with neutralizing VEGF antibody and GM6001 increased the TEER, which proves the involvement of VEGF and MMPs in the barrier-decreasing process. Analysis of occludin yielded decreased protein content and discontinuous expression at the endothelial cell borders under the influence of pericytes. These results together reveal the potential of pericytes to regulate the endothelial barrier integrity via MMPs and VEGF.
journal_name
Brain Resjournal_title
Brain researchauthors
Thanabalasundaram G,Pieper C,Lischper M,Galla HJdoi
10.1016/j.brainres.2010.05.096subject
Has Abstractpub_date
2010-08-06 00:00:00pages
1-10eissn
0006-8993issn
1872-6240pii
S0006-8993(10)01300-4journal_volume
1347pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The focal ischemia obtained in an animal model of middle cerebral artery occlusion (MCAo) causes the "core" of damage in the striatum and the "penumbra" of damage in the fronto-parietal cortex. The latter is mainly functionally affected and shows changes in nNOS and iNOS expression during the acute phase of ischemia. ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.11.029
更新日期：2007-02-09 00:00:00
abstract：:By application of the anterograde transport technique of Phaseolus vulgaris leuco-agglutinin the descending autonomic projection of the paraventricular hypothalamic nucleus was investigated. The Phaseolus lectin technique allowed the detection of the cells of origin in the paraventricular PVN, the precise position of ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90554-2
更新日期：1985-03-11 00:00:00
abstract：:The auditory cortical projections to the medial geniculate body (MGB) were studied in the cat. Lesions carefully restricted to each one of the subdivisions of the auditory and peri-auditory cortex were made and degenerating fibres were mapped in the MGB. In other experiments L-[4-5-3-H]leucine was injected into the co...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(75)90465-5
更新日期：1975-06-20 00:00:00
abstract：:Binding sites of atrial natriuretic peptide in the cerebral microvessels and choroid plexus of spontaneously hypertensive rats (SHR) and Wistar Kyoto control rats (WKY) were measured. In the microvessels, the number of ANP binding sites was lower in SHR than WKY, but there was no difference in affinity of binding site...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90984-j
更新日期：1990-06-04 00:00:00
abstract：:L-3, 4-Dihydroxyphenylalanine (L-dopa) has been widely used for the treatment of Parkinson's disease (PD), but its pharmacokinetics in the striatum have hardly been investigated, especially in primates. In this study, we examined the concentration of L-dopa in plasma and in the extracellular fluid (ECF) of the striatu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2003.08.065
更新日期：2003-12-12 00:00:00
abstract：:Acute hyperammonemia is associated with motor disturbances that are thought to involve striatal dopaminergic dysfunction. Discharge of striatal dopaminergic neurons is controlled by N-methyl-D-aspartate (NMDA) receptors, the excessive activation of which contributes to ammonia neurotoxicity. Here we show that ammonium...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02777-x
更新日期：2003-07-11 00:00:00
abstract：:Amyloid deposition, a histopathological feature of Alzheimer's disease brain, may be the underlying cause of this disease. The isolation of enzymes involved in both the normal and aberrant or alternative processing of the beta-amyloid precursor protein may lead to an understanding of how beta-protein, the major compon...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91279-n
更新日期：1992-09-04 00:00:00
abstract：:Axons in the adult mammalian CNS normally do not regenerate following axotomy even though they retain the capacity for growth under certain experimental conditions. Although this implies that the regeneration of adult axons is under regulative control, very little is known about the signaling pathways "responsible" fo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01468-3
更新日期：1996-04-01 00:00:00
abstract：:In the present study, we used an innovative music-rest interleaved fMRI paradigm to investigate the neural correlates of tinnitus distress. Tinnitus is a poorly-understood hearing disorder where individuals perceive sounds, in the absence of an external source. Although the great majority of individuals habituate to c...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2021.147277
更新日期：2021-01-07 00:00:00
abstract：:Unilateral injections of 1 microgram of kainic acid in the archistriatum of 5-day-old domestic chicks reduced fear, as expressed by distress calls (peeps), significantly more than did identical injections in the same structure on the left side. This result adds affective behavior to the growing list of functions that ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90861-9
更新日期：1986-07-09 00:00:00
abstract：:The effects of a conditioning lesion on the rate of sudomotor axon regeneration were judged by the recovery of sweat gland (SG) secretion after cholinergic stimulation. Three groups of mice were given a conditioning lesion by crushing the sciatic nerve at mid-thigh 4, 7, and 14 days before a test lesion. A 4th group r...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90547-o
更新日期：1990-02-19 00:00:00
abstract：:Evolutionary selection pressures have been one of the factors proposed to underlie sex differences in inhibitory control. Consequently, inhibitory control may vary as a function of the menstrual cycle and may be modulated by the stimuli being processed if these stimuli are related to reproductive success. We used func...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.05.061
更新日期：2008-08-11 00:00:00
abstract：:Our previous studies have indicated that oxygen radicals, produced during reoxygenation following short-term arterial hypoxia, lead to sustained suppression of cerebral arteriolar responses to N-methyl-D-aspartate (NMDA). However, whether arteriolar dilator responses to NMDA are reduced during arterial hypoxia has nev...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)01196-7
更新日期：1998-01-12 00:00:00
abstract：:GABA levels, high affinity GABA uptake and glutamic acid decarboxylase levels are reduced in rat ventroanterolateral thalamic nucleus after destruction of the entopeduncular nucleus with kainic acid. This is strong evidence that GABA is an entopedunculothalamic neurotransmitter. The striatoentopeduncular pathway is al...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90693-4
更新日期：1981-02-23 00:00:00
abstract：:There are sex differences in the neurochemistry of brainstem nuclei that participate in the control of breathing as well as sex differences in respiratory responses to hypoxia. Central chemoreception refers to the detection within the brain of minute changes in carbon dioxide (CO(2)) levels and the subsequent modulati...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.11.037
更新日期：2010-01-22 00:00:00
abstract：:Rapid skin indentations can produce strong tap-like sensations that contain little information about skin indentation depth. Although slower stimuli produce weaker sensations, subjects can more accurately identify how the position of the skin surface changes with respect to the deeper tissues. This dissociation betwee...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90609-7
更新日期：1984-09-10 00:00:00
abstract：:Nestin is an intermediate filament protein, transiently and abundantly expressed early in embryogenesis, e.g., in neuroepithelial cells, radial glia, germinal matrix cells and vascular cells. In the adult rat brain, nestin is only present in endothelial and select subventricular cells. We tested the hypothesis that af...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01502-4
更新日期：1999-08-14 00:00:00
abstract：:We previously reported that oral l-citrulline (l-Cit) administration antagonizes neuronal cell death in hippocampus following transient brain ischemia and that oral glutathione (GSH) administration prevents neuronal death through antioxidant activity. Here, we tested potential synergy of combined l-Cit and GSH adminis...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2017.03.014
更新日期：2017-05-15 00:00:00
abstract：:Among the many pathophysiologic consequences of traumatic brain injury are changes in catecholamines, including dopamine, epinephrine, and norepinephrine. In the context of TBI, dopamine is the one most extensively studied, though some research exploring epinephrine and norepinephrine have also been published. The pur...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2015.12.026
更新日期：2016-06-01 00:00:00
abstract：:Following extirpation of three quarters of the eye anlage of Xenopus laevis in stage 33/34, the remaining quadrants developed into small eyes of normal shape. Irrespective of the different portions of the eye anlage the quarter eyes originated from, the retinotectal projection was always confined to the rostrolateral ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(86)90089-1
更新日期：1986-09-01 00:00:00
abstract：:Global forebrain ischemia of 5-min duration results in delayed neuronal death (DND) of CA1 neurons in the gerbil hippocampus. DND can be prevented by a preconditioning sublethal ischemic stimulus (2. 5 min), a phenomenon, known as ischemic tolerance induction. Striking evidence exists for the involvement of regulatory...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02503-8
更新日期：2000-07-28 00:00:00
abstract：:Previous studies have demonstrated that food restriction during lactation extends the length of lactational infertility in rats. In order to determine whether Neuropeptide Y (NPY) plays a role in the increased length of lactational infertility seen in food-restricted rats, NPY immunoreactivity in the arcuate nucleus o...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00351-x
更新日期：1997-07-04 00:00:00
abstract：:PGP 9.5 is a new cytoplasmic neuron-specific protein structurally and immunologically distinct from neuron-specific enolase. A specific antiserum has now shown that this protein is widely distributed in vertebrate brains and is also present in cells of the human diffuse neuroendocrine system. PGP 9.5 appears to be old...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90241-x
更新日期：1983-11-14 00:00:00
abstract：:The excitatory amino acid L-glutamate injected into the nucleus of the solitary tract (NTS) in unanesthetized rats similar to peripheral chemoreceptor activation increases mean arterial pressure (MAP) and reduces heart rate. In this study, we investigated the effects of acute (1 day) and chronic (15 days) electrolytic...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.08.006
更新日期：2004-10-29 00:00:00
abstract：:Electroacupuncture (EA) has been reported to alleviate motor deficits in Parkinson's disease (PD) patients, and PD animal models. However, the mechanisms by which EA improves motor function have not been investigated. We have employed a 6-hydroxydopamine (6-OHDA) unilateral injection induced PD model to investigate wh...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2017.07.027
更新日期：2017-10-15 00:00:00
abstract：:Behavioral assessments of mouse models of neurodegenerative disorders are useful for investigating the molecular basis of the pathologies of the diseases. Here, we investigated the utility of a water-finding test using a video tracking system as a tool for evaluating cognitive deficits in Alzheimer's disease model mic...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.10.066
更新日期：2013-01-23 00:00:00
abstract：:Studies with radioactive antipyrene and deoxyglucose indicated that both blood flow and metabolism are enhanced in the goldfish optic tectum following a brief visual stimulation. It is concluded that the brain of cold-blooded animals demonstrates regionally regulated physiological alterations, previously reported only...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90195-1
更新日期：1979-01-26 00:00:00
abstract：:The distribution and relative specificity of cholecystokinin (CCK) receptors in the rat brain was mapped by in vitro autoradiography with [125I]CCK-33. We identified two distinct binding patterns, suggesting two CCK receptor types. The first is widespread and relatively non-specific. The second, localized to a few sub...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91413-7
更新日期：1986-01-01 00:00:00
abstract：:Extracellular and intracellular recordings have been made from non-pyramidal neurones in the rat hippocampus in vivo and in vitro. These cells were situated in the stratum pyramidale but were orthodromically activated with a lower threshold than pyramidal neurones in response to stimulation of the Schaffer collateral/...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)91235-6
更新日期：1984-02-20 00:00:00
abstract：:In the present study, we performed immunohistochemical studies to investigate the changes of Bcl-2 and Bax in the central nervous system of the transgenic mice expressing a human Cu/Zn SOD mutation. In contrast to the controls, a high density of Bcl-2-IR astrocytes were detected all around the gray matter of the spina...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)03014-6
更新日期：2000-12-29 00:00:00
