Culture of human outer root sheath cells from plucked hair follicles in serum-free conditions.

Abstract:

:We succeeded in culturing human outer root sheath cells (ORSC) in serum-free conditions without a biological feeder layer. The combination of collagen type IV substrate and modified MCDB 153 medium supplemented with bovine pituitary gland extract (K-GM medium) could support the growth of ORSC. These cells can be serially cultivated for at least 4 passages and stored in liquid nitrogen with good recovery. Thus, a large series of experiments using ORSC may be run simultaneously.

journal_name

J Dermatol Sci

authors

Arase S,Katoh S,Sadamoto Y,Nakanishi H,Urano Y,Fujie K,Takeda K

doi

10.1016/0923-1811(91)90045-y

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

66-70

issue

1

eissn

0923-1811

issn

1873-569X

pii

0923-1811(91)90045-Y

journal_volume

2

pub_type

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