Cyclophosphamide decreases the number, percentage and the function of CD25+ CD4+ regulatory T cells, which suppress induction of contact hypersensitivity.


BACKGROUND:It is well known that cyclophosphamide (Cy) treatment before sensitization paradoxically enhances rather than suppresses contact hypersensitivity (CH) reactions. In fact, Cy-treated mice developed a significant (p < 0.05) increase of the CH reactions to 2,4,6-trinitro-1-chrolobenzene (TNCB) in comparison with untreated mice. OBJECTIVE:In order to examine whether the target cells of Cy in the immuno-augmentative effect are CD25(+) CD4(+) regulatory T cells or not, we investigated effect of Cy treatment on CD25(+) CD4(+) T cells. METHOD:We examined Cy-treated CD25(+) CD4(+) T cells by flow cytometer and by inhibition assay on proliferation of CD25(-) CD4(+) T cells. RESULTS:Cy treatment remarkably reduced the number and percentage of CD25(+) CD4(+) T cells in the spleen and lymph nodes 3 and 5 days later. Moreover, CD25(+) CD4(+) T cells taken from the Cy-treated mice 3 days later showed the lower suppressive activity on proliferation of CD25(-) CD4(+) T cells, as compared to that from the untreated mice. Furthermore, transfer of CD25(+) CD4(+) T cells from untreated mice resulted in a significant decrease (p < 0.05) of the CH reactions enhanced by Cy treatment. CONCLUSION:These results indicate that enhancement of the CH reactions to TNCB by Cy treatment is attributed to the decrease in the number, percentage and the function of CD25(+) CD4(+) regulatory T cells.


J Dermatol Sci


Ikezawa Y,Nakazawa M,Tamura C,Takahashi K,Minami M,Ikezawa Z




Has Abstract


2005-08-01 00:00:00














  • Kojic acid-induced IL-6 production in human keratinocytes plays a role in its anti-melanogenic activity in skin.

    abstract:BACKGROUND:Kojic acid is a fungal metabolite widely used in medicinal and cosmetic formulations as a skin-lightening agent based on its de-pigmenting activity. Although in human clinical studies kojic acid has been shown to be effective in the treatment of hyper-pigmentation disorders such as melasma, the reasons for i...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Choi H,Kim K,Han J,Choi H,Jin SH,Lee EK,Shin DW,Lee TR,Lee AY,Noh M

    更新日期:2012-06-01 00:00:00

  • JSID Tanioku Memorial Lecture 1996. Genetic disorders of keratins and their associated proteins.

    abstract::It has recently been demonstrated that genetic defects in keratin genes cause a number of different skin disorders, including epidermolysis bullosa simplex (EBS), epidermolytic hyperkeratosis (EH), the EH form of epidermal nevi, epidermolytic and non-epidermolytic forms of palmoplantar keratoderma (EPPK and PPK) and p...

    journal_title:Journal of dermatological science



    authors: Fuchs E

    更新日期:1996-12-01 00:00:00

  • Growth stimulation of normal melanocytes and nevocellular nevus cells by gastrin releasing peptide (GRP).

    abstract::In order to know the possible effects of gastrin releasing peptide (GRP) on nevus cells and melanocytes, we studied the effect of GRP on the proliferation of cultured human nevus cells and normal melanocytes. MTS assay showed that GRP stimulated the growth of viable melanocytes at 1000 ng/ml. GRP also stimulated the g...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Terashi H,Itami S,Tadokoro T,Takeyama M,Katagiri K,Takayasu S

    更新日期:1998-06-01 00:00:00

  • Variable keratin polypeptide profile in human stratum corneum.

    abstract::Stratum corneum samples obtained from 46 members in three generations of seven families were analyzed for keratin pattern by gel electrophoresis. All these samples of apparently normal upper arm skin expressed the 55 kDa, 56.5 kDa, and 65 kDa keratin proteins; while only 28%, 20% and 48% of the samples expressed the 5...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Yaar M,Matoltsy GA,Gilchrest BA

    更新日期:1990-07-01 00:00:00

  • Photocontact dermatitis and chloracne: two major occupational and environmental skin diseases induced by different actions of halogenated chemicals.

    abstract::Among occupational and environmental disorders, contact or photocontact dermatitis and an acneiform eruption are two major skin disorders. Photocontact dermatitis was historically caused by various halogenated salicylanilides, while the acne is induced by halogenated aromatic hydrocarbons and thus called chloracne. Th...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审


    authors: Yamamoto O,Tokura Y

    更新日期:2003-08-01 00:00:00

  • Germline mutations of the PTCH gene in Japanese patients with nevoid basal cell carcinoma syndrome.

    abstract::Nevoid basal cell carcinoma syndrome (NBCCS) is an autosomal dominant disorder characterized by developmental and skeletal anomalies, palmo-plantar pits, odontogenic keratocysts, ectopic calcification, and occurrence of various types of tumors including basal cell carcinoma. Recent evidence has indicated that the huma...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Minami M,Urano Y,Ishigami T,Tsuda H,Kusaka J,Arase S

    更新日期:2001-09-01 00:00:00

  • Update on contact dermatitis 2007: Medical pearls from the annual meeting of the British Contact Dermatitis Society at the annual meeting of the British Association of Dermatologists.

    abstract::Contact dermatitis is an integral part of dermatology. Many patients affected by contact dermatitis suffer significant morbidity and require prompt diagnosis and treatment. Here we give an overview of contact dermatitis related presentations made during the British Contact Dermatitis Society at 87th annual meeting of ...

    journal_title:Journal of dermatological science



    authors: Alexandroff AB,Gawkrodger DJ

    更新日期:2008-09-01 00:00:00

  • Real-time vascular imaging and photodynamic therapy efficacy with micelle-nanocarrier delivery of chlorin e6 to the microenvironment of melanoma.

    abstract:BACKGROUND:Strategies combining anti-vascular therapy and vascular imaging may facilitate the prediction of early response and outcome in cancer treatment. OBJECTIVE:The aim of this study was to investigate the relationship between the tumor-associated vasculature in melanoma and to develop an approach for melanoma tr...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Lee CH,Lai PS,Lu YP,Chen HY,Chai CY,Tsai RK,Fang KT,Tsai MH,Hsu CY,Hung CC,Wu DC,Yu HS,Chang CH,Tsai DP

    更新日期:2015-11-01 00:00:00

  • Simultaneous and separated culture of keratinocytes and fibroblasts on each side of a collagen membrane.

    abstract::We have developed a new cell culture system which is of benefit for the research of dermal-epidermal interaction. In this system, normal human keratinocytes are cultured on the upper surface of a permeable collagen membrane, on the undersurface of which human fibroblasts are simultaneously and separately cultured in a...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Mitsuhashi Y,Mikami Y,Mikami H,Ishikawa H,Tamai K,Hashimoto I

    更新日期:1993-02-01 00:00:00

  • Epidermal basement membrane zone components: ultrastructural distribution and molecular interactions.

    abstract::The epidermal basement membrane zone (BMZ) comprises a multiprotein complex that aids the attachment of epidermal keratinocytes to the underlying dermis. In the last 5 years, our understanding of epidermal BMZ morphology, structure and function has dramatically improved. A complex network of molecular interactions has...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审


    authors: McMillan JR,Akiyama M,Shimizu H

    更新日期:2003-05-01 00:00:00

  • New method for determining prognosis of patients with psoriasis (E-PAP).

    abstract::We developed a new method for evaluating clinical symptoms of psoriasis during the observation period, by adding a parameter of time (the number of days) to the psoriasis area and severity index (PASI). This method was named the evaluation for prognosis with averaged PASI (E-PAP). Using this method, we assessed 14 cas...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Sugai J,Ozawa A,Kawakubo Y,Iizuka M,Miyahara M,Ohkido M

    更新日期:1998-01-01 00:00:00

  • Expression of inducible nitric oxide synthase in skin lesions of acute herpes zoster.

    abstract::Histopathologically, the skin lesions of acute herpes zoster (AHZ) are characterized by epidermal necrotic vesicles with inflammation. Nitric oxide (NO) is generated from L-arginine by nitric oxide synthase (NOS), and immune inflammation involves the activation of NOS in both effector cells and target cells. NO can ca...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Lim YJ,Chang SE,Choi JH,Sung KJ,Bahk JH,Do SH,Lee DS

    更新日期:2002-09-01 00:00:00

  • Therapeutic effects of streptococcal preparation OK-432 on atopic dermatitis-like lesions in NC/Nga mice: possible shift from a Th2- to Th1-predominance.

    abstract:BACKGROUND:The inducement of Th1 cell-mediated immune response, possibly brought about through bacterial stimulation, may serve to control atopic disorders such as atopic dermatitis (AD). The streptococcal preparation, OK-432, has been shown a potent Th1 inducer through the action of IL-12. NC/Nga mice under ordinary c...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Horiuchi Y,Bae S,Katayama I,Nishioka K

    更新日期:2004-09-01 00:00:00

  • Analysis of antigens recognized by autoantibodies in herpes gestationis. Usefulness of immunoblotting using a fusion protein representing an extracellular domain of the 180 kD bullous pemphigoid antigen.

    abstract::Herpes gestationis (HG) is a rare pregnancy-associated disease. The aim of this study was to compare various immunohistochemical and immunobiochemical techniques with respect to their diagnostic sensitivity for HG. We studied 43 HG sera; only half of these reacted with the basement membrane zone (BMZ) with both indire...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Murakami H,Amagai M,Higashiyama M,Hashimoto K,Chorzelski TP,Bhogal BS,Jenkins RE,Black MM,Zillikens D,Nishikawa T,Hashimoto T

    更新日期:1996-11-01 00:00:00

  • A study of anti-carbonic anhydrase II antibodies in rheumatic autoimmune diseases.

    abstract::Autoantibodies to human carbonic anhydrase II (CAII) were screened by ELISA in 109 sera from Asian Japanese patients with systemic lupus erythematosus (SLE), primary Sjögren's syndrome (Sjs), progressive systemic sclerosis (PSS) and dermatomyositis (DM). Anti-CAII antibodies were positive in 24.1% of SLE, 20.0% of pri...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Ono M,Ono M,Watanabe K,Miyashita Y,Inagaki Y,Ueki H

    更新日期:1999-11-01 00:00:00

  • Proteolytic digest derived from bovine Ligamentum Nuchae stimulates deposition of new elastin-enriched matrix in cultures and transplants of human dermal fibroblasts.

    abstract:BACKGROUND:Diverse topical products and injectable fillers used for correcting facial wrinkles induce rather short-lived effects because they target replacement of dermal collagen and hyaluronan, matrix components of limited biologic durability. OBJECTIVE:Present studies were aimed at stimulation of fully differentiat...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Hinek A,Wang Y,Liu K,Mitts TF,Jimenez F

    更新日期:2005-09-01 00:00:00

  • miR-128 targets the CC chemokine ligand 18 gene (CCL18) in cutaneous malignant melanoma progression.

    abstract:BACKGROUND:The CC chemokine ligand 18 (CCL18) has a higher expression in some tumors, while the CCL18 level can be a marker of tumor progression and prognosis. We previously reported that the expression of CCL18 gene was dramatically up-regulated in cutaneous malignant melanoma (CMM) and its expression levels were corr...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Song H,Tao Y,Ni N,Zhou X,Xiong J,Zeng X,Xu X,Qi J,Sun J

    更新日期:2018-09-01 00:00:00

  • Genetic variation at IL12B, IL23R and IL23A is associated with psoriasis severity, psoriatic arthritis and type 2 diabetes mellitus.

    abstract:BACKGROUND:Common DNA variants in IL12B, IL23R and IL23A have been associated with an increased susceptibility to psoriasis (Ps) and psoriatic arthritis (PsA). Metabolic comorbidities and cardiovascular risk factors have also been associated to both Ps and PsA. OBJECTIVE:To analyze the effect of single nucleotide poly...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Eirís N,González-Lara L,Santos-Juanes J,Queiro R,Coto E,Coto-Segura P

    更新日期:2014-09-01 00:00:00

  • Telomerase in cutaneous carcinogenesis.

    abstract::Telomerase is a ribonucleoprotein enzyme that catalyses the addition of telomeric repeats to telomeres. Since the shortening of telomeres is thought to act as a mitotic clock, activation of telomerase is crucial for the continued growth of cancer cells. Telomerase is frequently activated in premalignant and malignant ...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审


    authors: Ueda M

    更新日期:2000-03-01 00:00:00

  • Non-disulfided pro alpha 1(IV) chain in B16 melanoma cell culture.

    abstract::We have previously reported that two species of pro alpha 1(IV) collagen chains, disulfided (500 kDa) and non-disulfided (180 kDa), were produced by B16 melanoma cells (J Biochem (1994) 116; 1039-1043). The mechanism by which the non-disulfided pro alpha 1(IV) chain is produced was studied. No significant difference i...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Tajima S,Tokimitsu I

    更新日期:1996-10-01 00:00:00

  • Objective scratch monitor evaluation of the effect of an antihistamine on nocturnal scratching in atopic dermatitis.

    abstract::We previously reported a simple and easy-to-use device, Scratch Monitor for evaluation of nocturnal scratching. In the present study, the effect of an antihistamine (azelastine hydrochloride) on atopic dermatitis was investigated using this device. In 40 patients with atopic dermatitis, nocturnal scratching was measur...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Endo K,Sano H,Fukuzumi T,Adachi J,Aoki T

    更新日期:1999-12-01 00:00:00

  • Localization of fibronectin in the initial lymphatics and blood vessels of normal human skin: an immunoelectron microscopic investigation.

    abstract::The initial lymphatics of the human skin do not possess a basal lamina, but a subendothelial fibrous network, of which we do not know the biochemical and immunohistological composition. We investigated the wall of blood vessels and initial lymphatics in normal human skin for the presence of fibronectin by immunoelectr...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Lubach D,Nissen S,Marghescu S

    更新日期:1992-09-01 00:00:00

  • Genetic skin diseases related to desmosomes and corneodesmosomes.

    abstract::The integrity of the epidermis depends on the cohesion between keratinocytes, and desmosomes are the main adhesion structures. When cells become cornified, desmosomes are modified and transformed into corneodesmosomes. Mutations in the genes encoding desmosomal components underlie several skin diseases including palmo...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审


    authors: Ishida-Yamamoto A,Igawa S

    更新日期:2014-05-01 00:00:00

  • Mechanism of action of androgen in hair follicles.

    abstract::In order to investigate the mode of action of testosterone (T) on human hair follicles we studied the metabolism of T and localization of androgen receptors in outer root sheath cells (ORSC) and dermal papilla cells (DPC) from different body sites. T was principally metabolized to androstenedione (delta 4) even in bea...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Itami S,Sonoda T,Kurata S,Takayasu S

    更新日期:1994-07-01 00:00:00

  • Glyoxal-induced exacerbation of pruritus and dermatitis is associated with staphylococcus aureus colonization in the skin of a rat model of atopic dermatitis.

    abstract:BACKGROUND:Atopic dermatitis (AD) is a highly pruritic, chronic inflammatory skin disease associated with hyperreactivity to environmental triggers. Among those, outdoor air pollutants such as particulate matter (PM) have been reported to aggravate pre-existing AD. However, underlying mechanisms of air pollution-induce...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Han RT,Kim HY,Ryu H,Jang W,Cha SH,Kim HY,Lee J,Back SK,Kim HJ,Na HS

    更新日期:2018-06-01 00:00:00

  • Activation of fibroblast proliferation by Werner's syndrome fibroblast-conditioned medium.

    abstract::The effects of Werner's syndrome (WS) fibroblast-conditioned medium on cell proliferation and collagen production of normal fibroblasts were studied using four WS fibroblast strains. The conditioned medium from the WS fibroblasts brought about activation of normal fibroblast proliferation, whereas, that from late pass...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Hatamochi A,Arakawa M,Takeda K,Ueki H

    更新日期:1994-06-01 00:00:00

  • Detection of interferon-gamma mRNA in psoriatic epidermis by polymerase chain reaction.

    abstract::Psoriatic skin lesions contain HLA-DR positive T lymphocytes, and other activation antigens, which suggest that the T cells may be producing lymphokines. Gamma interferon is produced by activated T cells, and its presence in psoriasis has been inferred by the lesional keratinocyte expression of 3 gamma interferon-indu...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Barker JN,Karabin GD,Stoof TJ,Sarma VJ,Dixit VM,Nickoloff BJ

    更新日期:1991-03-01 00:00:00

  • Retinoid-induced beta-adrenergic inhibitory effect associated with increased thymidine incorporation of fetal rat keratinizing epidermal cells (FRSK cells).

    abstract::Using fetal rat keratinizing epidermal cells (FRSK cells) we investigated the effects of retinoids on the cyclic AMP system. The beta-adrenergic adenylate cyclase response was significantly decreased by the treatment of cells with 1 x 10(-6) M Ro 10-1670. The effect was observed by 12 h and remained for at least 48 h....

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Takahashi H,Iizuka H

    更新日期:1993-04-01 00:00:00

  • Expression of antimicrobial peptides in diabetic foot ulcer.

    abstract:BACKGROUND:Foot ulcers are one of the main diabetes complications due to its high frequency and difficulty of complete healing. There are several factors that participate in diabetic ulcers development and limited information exists about the role of antimicrobial peptides (AMP) in its pathogenesis. OBJECTIVE:The aim ...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Rivas-Santiago B,Trujillo V,Montoya A,Gonzalez-Curiel I,Castañeda-Delgado J,Cardenas A,Rincon K,Hernandez ML,Hernández-Pando R

    更新日期:2012-01-01 00:00:00

  • Propionibacterium acnes-induced iNOS and COX-2 protein expression via ROS-dependent NF-κB and AP-1 activation in macrophages.

    abstract:BACKGROUND:Propionibacterium acnes (P. acnes), a gram-positive anaerobic bacterium, plays a critical role in the development of inflammatory lesion as a result of cytokines production by keratinocytes and macrophages activation. However, effect of P. acnes on iNOS/NO and COX-2/PGE2 production in macrophages is still un...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Tsai HH,Lee WR,Wang PH,Cheng KT,Chen YC,Shen SC

    更新日期:2013-02-01 00:00:00