HPLC Estimation of berberine in Tinospora cordifolia and Tinospora sinensis.

Abstract:

:A high-performance liquid chromatographic method for the estimation of berberine in the stem of Tinospora cordifolia (Willd.) Miers. ex Hook.f. and Thoms. and Tinospora sinensis (Lour.) Merrill is described. The dried stems of T. cordifolia and T. sinensis were defatted with petroleum ether (60-80 degrees ). The marc was dried and further extracted with methanol. The concentration of berberine in methanol extract was determined using a C-18 reverse phase column with a mobile phase of acetonitrile:water (10:90 v/v) at a flow rate of 0.6 ml/min and with UV detection at 266 nm. TLC and HPLC comparison of both the species revealed significant variation in the chemical constitution of the two species. This observation becomes important in the context of the use of T. sinensis in place of the genuine drug T. cordifolia.

journal_name

Indian J Pharm Sci

authors

Srinivasan GV,Unnikrishnan KP,Rema Shree AB,Balachandran I

doi

10.4103/0250-474X.40341

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

96-9

issue

1

eissn

0250-474X

issn

1998-3743

journal_volume

70

pub_type

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