Gene cloning, expression, and characterization of a thermostable xylanase from Nesterenkonia xinjiangensis CCTCC AA001025.

abstract：:A cascade reaction combining the enzymatic hydrolysis of Penicillin G potassium salt (PGK) with the kinetically controlled enzymatic coupling of in situ formed 6-aminopenicillanic acid (6-APA) with p-hydroxyphenylglycine methyl ester (D-HPGM) to give amoxicillin as the final product by using a single enzyme has been d...

journal_title：Applied biochemistry and biotechnology

authors： Wu Q,Chen CX,Du LL,Lin XF

更新日期：2010-04-01 00:00:00

abstract：:In biotechnological processes, a great number of factors can influence the income productivity and conversion. Normally, it is not evident which of these factors are the most important and how they interact. In this work, multivariate analysis techniques are used as experimental design coupled to a detailed determinis...

journal_title：Applied biochemistry and biotechnology

authors： Lunelli BH,Rivera EC,Vasco de Toledo EC,Wolf Maciel MR,Maciel Filho R

更新日期：2008-03-01 00:00:00

abstract：:The production of biodiesel from Scenedesmus algal oil is one of the best alternative forms of liquid fuel production from biomass to petrol diesel. Biodiesel plays a significant role in the carbon sequestration process during cultivation. Scenedesmus algal species was isolated and cultured in a bold basal medium by u...

journal_title：Applied biochemistry and biotechnology

authors： Mamo TT,Mekonnen YS

更新日期：2020-04-01 00:00:00

abstract：:A series of 6-tosyl-4,5,6,7-tetrahydrothieno[2,3-c]pyridine-3-carboxamide analogues are synthesized by conventional techniques and characterized by elemental analysis, IR, MS, (1)H, and (13)C NMR. These are tested for their antibacterial activity against Bacillus subtilis (abbreviated as BS), Staphylococcus aureus (ab...

journal_title：Applied biochemistry and biotechnology

authors： Doshi H,Thakkar S,Khirsariya P,Thakur MC,Ray A

更新日期：2015-02-01 00:00:00

abstract：:As a part of a natural biological N-cycle, nitrification is one of the steps included in the conception of artificial ecosystems designed for extraterrestrial life support systems (LSS) such as Micro-Ecological Life Support System Alternative (MELiSSA) project, which is the LSS project of the European Space Agency. Ni...

journal_title：Applied biochemistry and biotechnology

authors： Farges B,Poughon L,Roriz D,Creuly C,Dussap CG,Lasseur C

更新日期：2012-07-01 00:00:00

abstract：:Chemical modification was evaluated to stabilize pig kidney D-amino acid oxidase (pkDAAO), which is required for analytical determination of D-amino acids. Optimization of modification conditions was performed to obtain high recovery yield and stability, and chemical modification at 30 degrees C for 12 h with a highly...

journal_title：Applied biochemistry and biotechnology

authors： Bakke M,Kajiyama N

更新日期：2004-03-01 00:00:00

abstract：:Glycopeptides produced by Streptomyces species are the drugs used against beta-lactam drug-resistant staphylococcal infections, and vancomycin is important among them. Increased prevalence of resistant strains increased the usage of vancomycin worldwide and also promoted attempts for indigenous production. The optimum...

journal_title：Applied biochemistry and biotechnology

authors： Padma PN,Rao AB,Yadav JS,Reddy G

更新日期：2002-07-01 00:00:00

abstract：:In this study, the prokaryotic expression system of Escherichia coli was used to modify prolyl aminopeptidase derived from Aspergillus oryzae JN-412 (AoPAP) via random mutagenesis and site-directed saturation mutagenesis. A random mutant library with a capacity of approximately 3000 mutants was compiled using error-pr...

journal_title：Applied biochemistry and biotechnology

authors： Liu D,Zhang D,Huang Q,Gu L,Zhou N,Tian Y

更新日期：2020-08-01 00:00:00

abstract：:In this work, important results from simulations are presented, showing the potentiality of the molecular distillation process for recovering vitamin E from vegetal oils. Two types of molecular distillators are considered: falling film and centrifugal. The results emphasize the degree of recovery and factors that infl...

journal_title：Applied biochemistry and biotechnology

authors： Batistella CB,Moraes EB,Maciel Filho R,Maciel MR

更新日期：2002-04-01 00:00:00

abstract：:The production of cellulase-free endoxylanase by the thermophilic fungus Thermomyces lanuginosus was investigated in semisolid fermentation and liquid fermentation. Different process variables were investigated in semisolid fermentation, employing corncob as the carbon source. The best results were with the following ...

journal_title：Applied biochemistry and biotechnology

authors： Damaso MC,Andrade CM,Pereira Júnior N

更新日期：2000-04-01 00:00:00

abstract：:The role of selenium (Se) supplementation on glutathione, a potent intracellular redox buffer, and trehalose, a well-known stress protectant molecule, was studied. The amount of glutathione decreased significantly while that of trehalose showed a minor decrease in the cells grown in Se-supplemented medium. After heat ...

journal_title：Applied biochemistry and biotechnology

authors： Sharma SC,Anand MS

更新日期：2006-04-01 00:00:00

abstract：:The effects of amino acid composition and peptide molecular mass on ACE-inhibitory and antioxidant activities of protein fragments obtained from tomato waste fermented using Bacillus subtilis were evaluated. The addition of B. subtilis increased the relative amounts of aromatic and positively-charged amino acids which...

journal_title：Applied biochemistry and biotechnology

authors： Moayedi A,Mora L,Aristoy MC,Hashemi M,Safari M,Toldrá F

更新日期：2017-01-01 00:00:00

abstract：:Polysaccharide glycosyl hydrolases are a group of enzymes that hydrolyze the glycosidic bond between carbohydrates or between a carbohydrate and a noncarbohydrate moiety. Here we illustrate that traditional schemes for grouping enzymes, such as by substrate specificity or by organism of origin, are not appropriate whe...

journal_title：Applied biochemistry and biotechnology

authors： Himmel ME,Karplus PA,Sakon J,Adney WS,Baker JO,Thomas SR

更新日期：1997-04-01 00:00:00

abstract：:Recombinant tissue-plasminogen activator (r-tPA), expressed in Escherichia coli cells in an aggregated form, was solubilized with a strong chaotrope in the absence of any reducing agent. The solubilized molecule was reactivated by a procedure that was developed to mimic the physiological conditions optimal for the fun...

journal_title：Applied biochemistry and biotechnology

authors： Grunfeld H,Patel A,Shatzman A,Nishikawa AH

更新日期：1992-05-01 00:00:00

abstract：:Growth kinetics and red pigment production of Monascus purpureus CCT 3802 was studied. A reproducible inoculum with extremely dispersed hyphae for bioreactor runs was obtained through a two-step cultivation in a shaker. First, the spores were cultivated in a complex medium rendering a suspension of vegetative cells. I...

journal_title：Applied biochemistry and biotechnology

authors： Pereira DG,Kilikian BV

更新日期：2001-04-01 00:00:00

abstract：:cDNA coding a prolyl aminopeptidase (PAP) was cloned from Aspergillus oryzae and over expressed in Bacillus subtilis with a 6×His tag in N-terminus. The recombinant prolyl aminopeptidase was secreted to extracellular by adding 2 mM CaCl2 and 5% D-sorbitol in TB medium; the enzyme activity in fermented supernatant incr...

journal_title：Applied biochemistry and biotechnology

authors： Wang KD,Wang KH,Zhou ND,Tian YP

更新日期：2017-04-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:Xylans are major hemicellulose components of plant cell wall which can be hydrolyzed by xylanolytic enzymes. Three forms of endo-β-1,4-xylanases (XynSW1, XynSW2A, and XynSW2B) produced by thermotolerant Streptomyces sp. SWU10 have been reported. In the present study, we described the expression and characterization of...

journal_title：Applied biochemistry and biotechnology

authors： Sukhumsirichart W,Deesukon W,Kawakami T,Matsumoto S,Seesom W,Sakamoto T

更新日期：2014-01-01 00:00:00

abstract：:In the present work, assays were performed to compare the efficacy of hypochlorous acid, chlorine dioxide, and ozone in the inactivation of Cryptosporidium oocyst in public water supply from Brazilian South conditions. Experiments were carried out in samples containing 2 x 10(4) oocysts/ml of C. parvum purified from f...

journal_title：Applied biochemistry and biotechnology

authors： Pereira JT,Costa AO,de Oliveira Silva MB,Schuchard W,Osaki SC,de Castro EA,Paulino RC,Soccol VT

更新日期：2008-12-01 00:00:00

abstract：:A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipett...

journal_title：Applied biochemistry and biotechnology

authors： Sato R,Takeyama H,Tanaka T,Matsunaga T

更新日期：2001-04-01 00:00:00

abstract：:Stachybotrys microspora is a filamentous fungus characterized by the secretion of multiple hydrolytic activities (cellulolytic and non-cellulolytic enzymes). The production of these biocatalysts was studied under submerged culture using glucose, cellulose, and wheat bran as carbon sources. Endoglucanases, pectinases, ...

journal_title：Applied biochemistry and biotechnology

authors： Abdeljalil S,Ben Hmad I,Saibi W,Amouri B,Maalej W,Kaaniche M,Koubaa A,Gargouri A

更新日期：2014-02-01 00:00:00

abstract：:The Institute of Gas Technology (IGT) conducted a pilot-scale study at a former manufactured gas plant (MGP) site in New Jersey. The objective of the study was to determine the effectiveness of an innovative chemical/biological treatment process (MGP-REM process) to remediate soils contaminated with polynuclear aromat...

journal_title：Applied biochemistry and biotechnology

authors： Pradhan SP,Paterek JR,Liu BY,Conrad JR,Srivastava VJ

更新日期：1997-04-01 00:00:00

abstract：:Agrobacterium isolated from soil samples produced two extracellular polysaccharides: succinoglycan, an acidic soluble polymer, and curdlan gum, a neutral, insoluble polymer. Maize glucose, cassava glucose, and maize maltose were used in fermentation medium to produce insoluble polysaccharide. Two Agrobacterium sp. str...

journal_title：Applied biochemistry and biotechnology

authors： Portilho M,Matioli G,Zanin GM,de Moraes FF,Scamparini AR

更新日期：2006-03-01 00:00:00

abstract：:Protein-A or goat anti-mouse-Ig (GAMIg) covalently bound to agarose-polyacrolein microsphere beads (APAMB) were employed for the removal of T cells from human peripheral blood leukocytes (PBL) and bone marrow (BM). The cell suspensions were treated with a monoclonal anti-T cell antibody (Leu-1) or monoclonal antilymph...

journal_title：Applied biochemistry and biotechnology

authors： Weiss L,Margel S,Slavin S

更新日期：1986-10-01 00:00:00

abstract：:Biodegradation is one of the important methods for the treatment of industrial wastewater containing aniline. In this paper, a degrading bacterium named MC-01, which could survive in high concentration aniline wastewater, was screened from industrial wastewater containing aniline and sludge. MC-01 was preliminarily id...

journal_title：Applied biochemistry and biotechnology

authors： Yang L,Ying C,Fang N,Zhong Y,Zhao-Xiang Z,Yun S

更新日期：2017-05-01 00:00:00

abstract：:Fed-batch method has shown a great promise in debottlenecking the high-solid enzymatic hydrolysis for the commercialization of cellulosic biosugar conversion for biofuel/biochemical production. To further improve enzymatic hydrolysis efficiency at high solid loading, fed-batch methods of green liquor-pretreated hardwo...

journal_title：Applied biochemistry and biotechnology

authors： He L,Han Q,Jameel H,Chang HM,Phillips R,Wang Z

更新日期：2018-04-01 00:00:00

abstract：:In a previous study, a recombination system based on the alpha complementation of cre recombinase and protein transduction was established. This system relied on the transient expression of the inactive, self-excisable C-terminal (beta) and the transduction of the N-terminal (alpha) cre fragments to cells as a purifie...

journal_title：Applied biochemistry and biotechnology

authors： Seidi A,Mie M,Kobatake E

更新日期：2009-08-01 00:00:00

abstract：:In this work, fibronectin purification from human plasma with the gelatin-immobilised poly(hydroxyethyl methacrylate) (PHEMA) cryogel has been evaluated. The PHEMA cryogel was prepared by cryo-polymerisation which proceeds in an aqueous solution of monomer frozen inside a plastic syringe. The PHEMA cryogel contained i...

journal_title：Applied biochemistry and biotechnology

authors： Perçin I,Aksöz E,Denizli A

更新日期：2013-09-01 00:00:00

abstract：:Hydrogels are attractive biomaterials for three-dimensional cell culture and tissue engineering applications. The preparation of hydrogels using alginate and gelatin provides cross-linked hydrophilic polymers that can swell but do not dissolve in water. In this work, we first reinforced pure alginate by using polyoxye...

journal_title：Applied biochemistry and biotechnology

authors： Aroguz AZ,Baysal K,Adiguzel Z,Baysal BM

更新日期：2014-05-01 00:00:00

abstract：:The production of a thermophilic 2-deoxyribose-5-phosphate aldolases (DERA) in Escherichia coli BL21 under continuous lactose induction strategy was investigated. The process was combined with the exponential feeding method, controlling the feeding rate to maintain the specific growth rate at 0.15 h(-1). The results i...

journal_title：Applied biochemistry and biotechnology

authors： Pei XL,Wang QY,Li CL,Qiu XF,Xie KL,Huang LF,Wang AM,Zeng ZW,Xie T

更新日期：2011-09-01 00:00:00