Abstract：
BACKGROUND:Uterine serous papillary carcinoma (USPC) is a biologically aggressive variant of endometrial cancer. We investigated the expression of Serum Amyloid A (SAA) and evaluated its potential as a serum biomarker in USPC patients. METHODS:SAA gene and protein expression levels were evaluated in USPC and normal endometrial tissues (NEC) by real-time PCR, immunohistochemistry (IHC), flow cytometry and by a sensitive bead-based immunoassay. SAA concentration in 123 serum samples from 51 healthy women, 42 women with benign diseases, and 30 USPC patients were also studied. RESULTS:SAA gene expression levels were significantly higher in USPC when compared with NEC (mean copy number by RT-PCR=162 vs 2.21; P=0.0002). IHC revealed diffuse cytoplasmic SAA protein staining in USPC tissues. High intracellular levels of SAA were identified in primary USPC cell lines evaluated by flow cytometry and SAA was found to be actively secreted in vitro. SAA concentrations (mug ml(-1)) had a median (95% CIs) of 6.0 (4.0-8.9) in normal healthy females and 6.0 (4.2-8.1) in patients with benign disease (P=0.92). In contrast, SAA values in the serum of USPC patients had a median (95% CI) of 15.6 (9.2-56.2), significantly higher than those in the healthy group (P=0.0005) and benign group (P=0.0006). Receiver operating characteristics (ROC) analysis of serum SAA to classify advanced- and early-stage USPC yielded an area under the ROC curve of 0.837 (P=0.0024). CONCLUSION:SAA is not only a liver-secreted protein but is also a USPC cell product. SAA may represent a novel biomarker for USPC to assist in staging patients preoperatively, and to monitor early-disease recurrence and response to therapy.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Cocco E,Bellone S,El-Sahwi K,Cargnelutti M,Casagrande F,Buza N,Tavassoli FA,Siegel ER,Visintin I,Ratner E,Silasi DA,Azodi M,Schwartz PE,Rutherford TJ,Pecorelli S,Santin ADdoi
10.1038/sj.bjc.6605129subject
Has Abstractpub_date
2009-07-21 00:00:00pages
335-41issue
2eissn
0007-0920issn
1532-1827pii
6605129journal_volume
101pub_type
杂志文章abstract：:The identification of peptide vaccine candidates to date has been focused on human leukocyte antigen (HLA)-A2 and -A24 alleles. In this study, we attempted to identify cytotoxic T lymphocyte (CTL)-directed Lck-derived peptides applicable to HLA-A11(+), -A31(+), or -A33(+) cancer patients, because these HLA-A alleles s...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604071
更新日期：2007-12-17 00:00:00
abstract：:Studies in animal tumour models of colorectal cancer suggest that F(ab')2 antibody fragments to carcinoembryonic antigen (CEA) labelled with iodine-131 give superior therapy compared with intact anti-CEA antibody. The purpose of this study was to investigate this hypothesis in patients. Ten patients received intact A5...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/bjc.1994.338
更新日期：1994-09-01 00:00:00
abstract：BACKGROUND:Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) contains crucial information about tumour heterogeneity and the transport limitations that reduce drug efficacy. Mathematical modelling of drug delivery and cellular responsiveness based on underutilised DCE-MRI data has the unique potential to p...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6605773
更新日期：2010-08-10 00:00:00
abstract：BACKGROUND:Combined-modality therapy is a promising approach to improve the therapeutic index of radiotherapy. However, these improvements could come at the cost of increased toxicities. Clinical trials evaluating anti-tumour efficacy of bevacizumab combined with radiotherapy have encountered unexpected side effects. T...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2012.236
更新日期：2012-07-10 00:00:00
abstract：:Human peripheral-blood mononuclear cells, separated by Isopaque-Ficoll flotation and E-rosette formation, were tested by the fluorescein fluorescence polarization method of Cercek & Cercek (the SCM test). The response to stimulation with PHA or cancer tissue leads to a decreased polarization value TP). The responding ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1979.228
更新日期：1979-10-01 00:00:00
abstract：:Protein kinase B/Akt has been described as a central mediator of anti-apoptotic signals transduced by the PI3 kinase. Although the role of Akt in the suppression of apoptosis is well elucidated, a potential function of Akt in tumorigenesis and chemoresistance is less intensively documented. In this study, we describe ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600566
更新日期：2002-10-07 00:00:00
abstract：:Serum laminin P1 was studied in patients with small cell lung cancer (SCLC), non-small cell lung cancer (NSCLC), respiratory infections, pulmonary fibrosis, and in normal subjects. The level of serum laminin P1 was elevated (greater than 1.27 U ml-1) in 58.9% of SCLC and in 11.5% of NSCLC patients. Median value in SCL...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1992.123
更新日期：1992-04-01 00:00:00
abstract：OBJECTIVE:Detection of lymph node involvement in women with IB2-IIB cervical cancer could have a positive effect on survival. We set out to evaluate the incidence of pelvic and/or para-aortic lymph node involvement using the sentinel node (SN) biopsy and its impact on survival. METHODS:From 2002 to 2010, 66 women with...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2011.541
更新日期：2012-01-03 00:00:00
abstract：:The tolerability, anti-tumour activity and pharmacokinetic interaction of high-dose intravenous cyclosporin combined with intravenous etoposide was evaluated in children. Eighteen patients with recurrent or refractory tumours, all of whom had previously received etoposide, were treated with a combination of high-dose ...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/bjc.1998.383
更新日期：1998-06-01 00:00:00
abstract：:TNF-related apoptosis-inducing ligand (TRAIL, also known as Apo-2L) is a promising novel anticancer agent that selectively induces apoptosis in tumour cells and the activity of which can be enhanced by combined treatment with chemo- or radiotherapy. For therapeutic purposes, the use of full-length TRAIL may be favoura...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601910
更新日期：2004-07-05 00:00:00
abstract：:Secondary bile acids produced by the action of the colonic microflora may increase risk of colorectal cancer. Serum bile acid concentrations reflect the faecal bile acid profile and may be of value as biomarkers of risk of colorectal cancer. In a pilot investigation we examined: (i) the reproducibility of measurements...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600340
更新日期：2002-06-05 00:00:00
abstract：:Dendritic cells (DC) are professional antigen-presenting cells that play a pivotal role in the induction of immunity. Ex vivo-generated, tumour antigen-loaded mature DC are currently exploited as cancer vaccines in clinical studies. However, antigen loading and maturation of DC directly in vivo would greatly facilitat...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603341
更新日期：2006-10-09 00:00:00
abstract：:Using the DMBA-induced mammary tumour as a model, the effect of hormone manipulation on steroid sulphurylation and on oxidative and reductive metabolism has been investigated. Oestradiol-17 beta, or oestradiol-17 beta + progesterone, administered to oophorectomized animals, had no effect on adenosine-3'-phosphate-5'-p...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1980.14
更新日期：1980-01-01 00:00:00
abstract：BACKGROUND:Multiple early gastric cancers (EGCs) may develop in 6-14% of patients even after achieving curative endoscopic submucosal dissection (ESD); however, a useful biomarker for predicting recurrence is not available. The present study investigated whether the expression of CD44 variant 9 (CD44v9), a functional c...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.314
更新日期：2013-07-23 00:00:00
abstract：:Actin has been measured in subcellular fractions from Rat-1 fibroblasts and in Rous sarcoma virus-transformed Rat-1 cells (VIT), using the DNase 1 inhibition assay. The transformed cells showed a significant shift in the actin monomer (G)in equilibrium with polymer (F) equilibrium within the cell cytosol, and a signif...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1986.74
更新日期：1986-04-01 00:00:00
abstract：:The radiosensitizing activity of S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide (NO) donor, was assessed in a model of non-metabolic hypoxia achieved in an atmosphere of 95% nitrogen-5% carbon dioxide. A 10 min preincubation of hypoxic EMT-6 cells (10 x 10(6) ml(-1)) with 0.1 and 1 mM SNAP before radiation res...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690173
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND:This phase 1 study examined the safety, tolerability, pharmacokinetics and preliminary efficacy of eribulin-liposomal formulation (eribulin-LF) in patients with advanced solid tumours. METHODS:Eligible patients with ECOG PS 0-1 were treated with eribulin-LF either on day 1 every 21 days (Schedule 1), or on ...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1038/s41416-019-0377-x
更新日期：2019-02-01 00:00:00
abstract：:A comparative study has been made of fibroblasts obtained from patients with differing susceptibilities to malignant disease, both with respect to their chromosome complements and their transformation with SV40 virus. Fibroblasts from 2 Bloom's syndrome patients were found not to have raised SV40 transformation rates ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1977.235
更新日期：1977-11-01 00:00:00
abstract：:We have purified epithelial cells from human colonic tumours by velocity sedimentation in an isokinetic density gradient of Ficoll in tissue culture medium. In frozen sections of colonic carcinoma, histochemically demonstrable N-acetyl-beta-D-glucosaminidase (HDAG) was observed primarily in epithelial cells. We used t...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1977.128
更新日期：1977-06-01 00:00:00
abstract：:The aims of this study were to identify prognostic variables for toxicity and survival in patients with cancer participating in phase I clinical trials and compare characteristics of those treated with cytotoxic chemotherapy (CT) and non-cytotoxic drugs (non-CT). Data were collected from 420 (114 CT, 306 non-CT) patie...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601218
更新日期：2003-10-06 00:00:00
abstract：:This study explored the relationships of serum insulin-like growth factors, IGF-I and IGF-II, and their binding proteins (IGFBP)-2 and IGFBP-3, with key clinicopathological parameters in 92 patients with colorectal cancer (cases). Comparisons were made with 57 individuals who had a normal colonoscopy (controls). Seria...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2000.1462
更新日期：2000-11-01 00:00:00
abstract：:In a population-based prospective study of 19,998 Japanese individuals, consumption of vegetables, green-yellow and green leafy vegetables was inversely associated with the risk of hepatocellular carcinoma (101 cases), with multivariable hazard ratios for the highest vs lowest tertile of 0.61 (95% confidence interval ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604843
更新日期：2009-01-13 00:00:00
abstract：:The combination of 5-fluorouracil (5-FU) and interferon-alpha (IFN-alpha) has reported activity in the treatment of advanced colorectal carcinoma. Laboratory studies of IFN-beta suggest that this agent may offer theoretical advantages over IFN-alpha in combination with 5-FU. A total of 27 patients with advanced or rec...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/bjc.1997.69
更新日期：1997-01-01 00:00:00
abstract：:Two-tumour-cell-aggregation factors derived from rat ascites hepatoma cells had different antigenicity; one, with a strong potency, was not absorbed by immunoadsorbent chromatography with anti-rat serum antibody and the other, with a weak potency, was. The unabsorbed factor possessed mitogenic activity on lymphocytes ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.192
更新日期：1978-08-01 00:00:00
abstract：BACKGROUND:The recognition that cancer is not a single entity, rather that different cancers have different causes and trajectories, has been a key development in the scientific understanding of cancer. However, little is known about the British public's awareness of differences between cancers. This study examined dif...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2012.185
更新日期：2012-06-05 00:00:00
abstract：:A case-control study from Uganda found that the risk of Kaposi's sarcoma increased with increasing titre of antibodies against Kaposi's sarcoma-associated herpesvirus (KSHV) latent nuclear antigens, independently of HIV infection. Clinically, widespread Kaposi's sarcoma was more frequent among patients with HIV infect...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601113
更新日期：2003-08-04 00:00:00
abstract：:When L2C leukaemic B lymphocytes from guinea-pigs were incubated in vitro with antibody directed to their surface immunoglobulin (Ig), a rapid rise in intracellular adenosine 3':5'-phosphate (cyclic adenosine monophosphate, cAMP) was observed. Estimation of cAMP was by a protein-binding assay using bovine adrenal prot...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1979.77
更新日期：1979-04-01 00:00:00
abstract：:To evaluate whether green tea consumption provides protection against stomach cancer death, relative risks were calculated using Cox proportional hazards regression analysis in the Japan Collaborative Study for Evaluation of Cancer Risk, sponsored by the Ministry of Health and Welfare (JACC Study). The study was based...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600487
更新日期：2002-07-29 00:00:00
abstract：:We examined the association of diet with insulin-like growth factors (IGF) in 344 disease-free men. Raised levels of IGF-I and/or its molar ratio with IGFBP-3 were associated with higher intakes of milk, dairy products, calcium, carbohydrate and polyunsaturated fat; lower levels with high vegetable consumption, partic...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600946
更新日期：2003-06-02 00:00:00
abstract：BACKGROUND:Complete tumour response (pCR) to neo-adjuvant chemo-radiotherapy for rectal cancer is associated with a reduction in local recurrence and improved disease-free and overall survival, but is achieved in only 20-30% of patients. Drug repurposing for anti-cancer treatments is gaining momentum, but the potential...
journal_title：British journal of cancer
pub_type： 杂志文章,meta分析
doi：10.1038/bjc.2017.175
更新日期：2017-07-11 00:00:00
