Abstract:
:A comparative study of histochemical detection of eosinophils in fixed murine tissue is lacking. Five histochemical methods previously reported for eosinophil detection were quantitatively and qualitatively compared in an established murine RSV vaccine-enhanced inflammation model. Nonspecific neutrophil staining was evaluated in tissue sections of neutrophilic soft tissue lesions and bone marrow from respective animals. Eosinophils had granular red to orange-red cytoplasmic staining, depending on the method, whereas neutrophils had, when stained, a more homogenous cytoplasmic pattern. Nonspecific background staining of similar coloration was variably seen in vascular walls and erythrocytes. Astra Blue/Vital New Red, Congo Red, Luna, Modified Hematoxylin and Eosin, and Sirius Red techniques were all effective in detecting increased eosinophil recruitment compared to controls; however, differences in eosinophil quantification varied significantly between techniques. Astra Blue/Vital New Red had the best specificity for differentiating eosinophils and neutrophils but had a reduced ability to enumerate eosinophils and was the most time intensive. The Luna stain had excessive nonspecific staining of tissues and a reduced enumeration of infiltrating eosinophils, which made it suboptimal. For multiple parameters such as eosinophil detection, specificity, and contrast with background tissues, the Sirius Red followed by Congo Red and Modified Hematoxylin and Eosin methods were useful, each with their own staining qualities.
journal_name
Toxicol Patholjournal_title
Toxicologic pathologyauthors
Meyerholz DK,Griffin MA,Castilow EM,Varga SMdoi
10.1177/0192623308329342subject
Has Abstractpub_date
2009-02-01 00:00:00pages
249-55issue
2eissn
0192-6233issn
1533-1601pii
0192623308329342journal_volume
37pub_type
杂志文章abstract::Swine, especially the miniature swine or minipigs, are increasingly being used in preclinical safety assessment of small molecules, biopharmaceutical agents, and medical devices as an alternate nonrodent species. Although swine have been used extensively in biomedical research, there is a paucity of information in the...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623315611762
更新日期:2016-04-01 00:00:00
abstract::The identification of adverse health effects has a central role in the development and risk/safety assessment of chemical entities and pharmaceuticals. There is currently a need for better alignment regarding how nonclinical adversity is determined and characterized. The European Society of Toxicologic Pathology (ESTP...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623316642527
更新日期:2016-08-01 00:00:00
abstract::Alanine aminotransferase (ALT) is used as an indicator of hepatocellular injury. Since ALT consists of two isoenzymes, a better understanding of ALT isoenzyme biology in response to compounds that cause metabolic adaptive versus hepatotoxic responses will allow for a more accurate assessment of the significance of an ...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623312447550
更新日期:2012-12-01 00:00:00
abstract::The microbiome is increasingly recognized as a critical component in human development, health, and disease. Its relevance to toxicology and pharmacology involves challenges to current concepts related to absorption, metabolism, gene:environment, and pathways of response. Framing testable hypotheses for experimental a...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/0192623316672073
更新日期:2017-01-01 00:00:00
abstract::In preclinical safety studies, drug-induced vascular injury can negatively impact candidate-drug selection because there are no obvious diagnostic markers for monitoring this pathology preclinically or clinically. Furthermore, our current understanding of the pathogenesis of this lesion is limited. While vasodilatatio...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1080/01926230500512076
更新日期:2006-01-01 00:00:00
abstract::Our previous studies showed that protein kinase Cepsilon (PKCepsilon) verexpression in mouse skin resulted in metastatic squamous cell carcinoma (SCC) elicited by single 7,12-dimethylbenz(a)anthracene (DMBA)-initiation and 12-O-tetradecanoylphorbol-13-acetate (TPA)-promotion in the absence of preceding papilloma forma...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1080/01926230701748164
更新日期:2007-12-01 00:00:00
abstract::Recent advances in immunocytochemical techniques allow the localization of specific antigens in tissue sections. The work reported here attempts to evaluate the application of antibody-labeled, disease-related protein, followed by microscopy and computerized image analysis. Using an experimental anti-tumor, polyclonal...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262338701500308
更新日期:1987-01-01 00:00:00
abstract::The definition of diagnostic thresholds is an important aspect of identification and recording of histopathologic lesions in toxicology studies. Although the primary goal of the pathology examination is to identify and interpret lesions associated with the administration of the test article, the toxicologic pathologis...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623312443322
更新日期:2012-10-01 00:00:00
abstract::The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) outbreak that started in Wuhan, China, in 2019 resulted in a pandemic not seen for a century, and there is an urgent need to develop safe and efficacious vaccines. The scientific community has made tremendous efforts to understand the disease, and unpara...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/0192623320959090
更新日期:2020-10-01 00:00:00
abstract::The effects of oral contraceptives have been studied in the beagle bitch for periods up to 7 yr. High doses of these potent estrogen: progestogen (E:P) combinations have been shown to promote tumors in the mammary glands, smooth muscle of the tubular genitalia, and occasionally in the transitional epithelium of the ne...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/019262338901700216
更新日期:1989-01-01 00:00:00
abstract::The usefulness of isolated adult cardiac myocytes, both in suspension and in culture, as a model system for measuring cardiotoxicity is evaluated. It has been suggested that isolated adult myocytes should preferably be cultured for such experimentation, as this restores the cells to a physiological preference for fatt...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Subacute inhalation study (1 week or 2 weeks) is an important process for screening out inhaled compounds causing lung irritation. To investigate whether the lung weight can be used as an indicator for acute lung injury, we have analyzed retrospectively the lung weight data from 30 studies in rats exposed to dry powde...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623312470763
更新日期:2013-08-01 00:00:00
abstract::A total of 75 BALB/cStCrlfC3H/Nctr male weanling mice were administered either 0 or 250 ppm of 4 ethylsulfonylnaphthalene-1-sulfonamide (ENS) in the diet for periods up to 14 days to evaluate the early morphological changes of the transitional epithelium of the urinary bladder with scanning (SEM) and transmission (TEM...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262338601400302
更新日期:1986-01-01 00:00:00
abstract::Progressive renal interstitial fibrosis occurs following tissue injury, resulting in chronic renal failure. In the fibrogenesis, macrophages are speculated to induce myofibroblasts producing matrix protein. The kinetics of these cells in renal fibrosis induced in rats by repeated injection of cisplatin (CDDP) (2 mg/kg...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339602400208
更新日期:1996-03-01 00:00:00
abstract::In the constantly evolving field of toxicologic pathology, a pathologist's career is often characterized by multiple career transitions. However, these transitions can be challenging and/or overwhelming and may require a shift in focus, strategic approach, and acquisition of new skills and expertise. In order to provi...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623317736299
更新日期:2017-10-01 00:00:00
abstract::We have previously indicated that the ideal animal tumor model should mimic the human disease. This means that the investigator should be able to ascertain the influence of host factors on the initiation of tumorigenesis, mimic the susceptibility of tumor response based on age and reproductive history, and determine t...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/0192623314532036
更新日期:2015-02-01 00:00:00
abstract::High-resolution digitalizing of histology slides facilitates the development of computational alternatives to manual quantitation of features of interest. We developed a MATLAB-based quantitative histological analysis tool (QuHAnT) for the high-throughput assessment of distinguishable histological features. QuHAnT val...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623314544379
更新日期:2015-04-01 00:00:00
abstract::Nephrotoxicity is one of the more common causes of attrition in nonclinical drug development. Like most tissues, the kidney has a limited number of ways of responding to toxicological insults from diverse mechanistic pathways, which can limit the ability to determine mechanisms of renal injury using the assays routine...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623318788302
更新日期:2018-08-01 00:00:00
abstract::Direct tissue profiling and imaging mass spectrometry (MS) allow for detailed mapping of the complex protein pattern across a tissue sample. Utilization of these tools provides spatial information across a tissue section for target protein expression and can be used to correlate changes in expression levels with speci...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1080/01926230590881862
更新日期:2005-01-01 00:00:00
abstract::Histopathology of the reproductive system of 76 young adult Dutch Belted and 22 young adult or adult male New Zealand White rabbits was conducted as part of fertility studies. Thirty-five (46%) of the Dutch Belted and 11 (50%) of the New Zealand White males had multifocal vesicular gland basal epithelial cell atypia a...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262338501300309
更新日期:1985-01-01 00:00:00
abstract::To determine if additive or synergistic toxic effects would occur, adult male rats were exposed orally to lead carbonate (2,000 mg/kg) for 9 days before trichloroethylene (TCE), 2,000 mg/kg, was given concurrently for an additional 7 days. Comparisons were made with groups of vehicle-treated rats and rats given only l...
journal_title:Toxicologic pathology
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doi:10.1080/01926230152499809
更新日期:2001-07-01 00:00:00
abstract::In a simulated fire-related smoke exposure protocol, New Zealand white rabbits were utilized to investigate the potential effects of the 21-aminosteroid (lazaroid) analog U75412E on the early events of acute lung injury. Inhalation of a total of 1.6 mg/kg U75412E aerosolized at a rate of 1.53 mg/min at 0.5 hr after sm...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339902700309
更新日期:1999-05-01 00:00:00
abstract::Many common inflammatory disorders are characterized by the infiltration of neutrophils across epithelial lined (mucosal) surfaces resulting in disruption of critical barrier function that protects from microbes and noxious agents. In such conditions, disease symptoms are complex but directly related to leukocyte effe...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/0192623313511336
更新日期:2014-01-01 00:00:00
abstract::The evaluation of neurotoxic damage involves a unique set of challenges. Vulnerable structures, such as neocortex, hippocampus, spinal cord, and peripheral nerve are complex and sharply differentiated; deficits can result from insults to one or more element(s) in the system (e.g., myelin, axon, soma, synapse, or glia)...
journal_title:Toxicologic pathology
pub_type: 杂志文章,评审
doi:10.1177/0192623310390231
更新日期:2011-01-01 00:00:00
abstract::Lung cancer is the most common cause of cancer-related deaths in humans worldwide. There is strong evidence that the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and its metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) play an important role in carcinogenesis caused ...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623317751573
更新日期:2018-02-01 00:00:00
abstract::This study aimed to evaluate the carcinogenic potential of the herbicide Diuron in a two-stage rat medium-term mammary carcinogenesis model initiated by 7,12-dimethylbenz(a)anthracene (DMBA). Female seven-week-old Sprague-Dawley (SD) rats were allocated to six groups: groups G1 to G4 received intragastrically (i.g.) a...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623310396904
更新日期:2011-04-01 00:00:00
abstract::Investigations in toxicologic neuropathology are complex undertakings because of the intricate spatial and temporal diversity in the anatomic, functional, and molecular organization of the central and peripheral nervous systems. This compilation of toxicologic neuropathology resources has been designed to consolidate ...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623310385142
更新日期:2011-01-01 00:00:00
abstract::Minoxidil, a long-acting vasodilating antihypertensive agent, reduces arterial blood pressure by a direct action on arteriolar smooth muscle. The present studies examined the gross anatomic, histologic and ultrastructural myocardial alterations produced by 2 daily doses of minoxidil in beagle dogs (0.5, 1, or 3 mg/kg)...
journal_title:Toxicologic pathology
pub_type: 杂志文章
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更新日期:1989-01-01 00:00:00
abstract::Duplications of the gastrointestinal tract are exceedingly rare in laboratory animals. We report a case of a communicating intestinal duplication in a 17-wk-old Sprague-Dawley (SD) rat. The duplication was present in the mesenteric border of the ileum, and both proximal and distal ends were communicated with the lumen...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/019262339802600216
更新日期:1998-03-01 00:00:00
abstract::Over the past decade, laser capture microdissection (LCM) has grown as a tool for gene expression profiling of small numbers of cells from tumor samples and of specific cell populations in complex tissues. LCM can be used to study toxicant effects on selected cell populations within the testis at different stages of s...
journal_title:Toxicologic pathology
pub_type: 杂志文章
doi:10.1177/0192623314526319
更新日期:2014-12-01 00:00:00