Abstract:
:This study aimed to evaluate the carcinogenic potential of the herbicide Diuron in a two-stage rat medium-term mammary carcinogenesis model initiated by 7,12-dimethylbenz(a)anthracene (DMBA). Female seven-week-old Sprague-Dawley (SD) rats were allocated to six groups: groups G1 to G4 received intragastrically (i.g.) a single 50 mg/kg dose of DMBA; groups G5 and G6 received single administration of canola oil (vehicle of DMBA). Groups G1 and G5 received a basal diet, and groups G2, G3, G4, and G6 were fed the basal diet with the addition of Diuron at 250, 1250, 2500, and 2500 ppm, respectively. After twenty-five weeks, the animals were euthanized and mammary tumors were histologically confirmed and quantified. Tumor samples were also processed for immunohistochemical evaluation of the expressions of proliferating cell nuclear antigen (PCNA), cleaved caspase-3, estrogen receptor-α (ER-α), p63, bcl-2, and bak. Diuron treatment did not increase the incidence or multiplicity of mammary tumors (groups G2 to G4 versus Group G1). Also, exposure to Diuron did not alter tumor growth (cell proliferation and apoptosis indexes) or immunoreactivity to ER-α, p63 (myoephitelial marker), or bcl-2 and bak (apoptosis regulatory proteins). These findings indicate that Diuron does not have a promoting potential on mammary carcinogenesis in female SD rats initiated with DMBA.
journal_name
Toxicol Patholjournal_title
Toxicologic pathologyauthors
Grassi TF,Rodrigues MA,de Camargo JL,Barbisan LFdoi
10.1177/0192623310396904subject
Has Abstractpub_date
2011-04-01 00:00:00pages
486-95issue
3eissn
0192-6233issn
1533-1601pii
0192623310396904journal_volume
39pub_type
杂志文章abstract::Many new observations have been made correlating changes in renal morphology with changes in renal function. These changes have led to a better definition of the various segments which make up the renal tubule. These morphological patterns vary in different species as is documented by morphologic studies and by differ...
journal_title:Toxicologic pathology
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journal_title:Toxicologic pathology
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journal_title:Toxicologic pathology
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journal_title:Toxicologic pathology
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abstract::A spontaneous multicystic peritoneal mesothelioma was detected in a 108-wk-old male Fischer-344/DuCrj rat. Grossly, a tumor containing numerous, semi-transparent, variously sized, thin-walled cysts was found on the splenic serosal surface. Microscopically, each cyst was surrounded by variable amounts of loose connecti...
journal_title:Toxicologic pathology
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journal_title:Toxicologic pathology
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journal_title:Toxicologic pathology
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journal_title:Toxicologic pathology
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journal_title:Toxicologic pathology
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journal_title:Toxicologic pathology
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journal_title:Toxicologic pathology
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journal_title:Toxicologic pathology
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journal_title:Toxicologic pathology
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