Abstract:
:A new resource of biocatalyst for asymmetric reduction of aromatic ketones has been discovered for the first time from a common plant seed, adzuki bean, i.e. Phaseolus angularis (Willd.) W.F. Wight. The study investigated the best methods to prepare the biocatalyst and its ability to reduce ketones. Our results indicated that the biocatalyst from adzuki bean could reduce various aromatic ketones at relatively high concentrations (e.g. 100mM), exhibiting excellent stereoselectivity (>98% e.e.). In addition, it was found that NADPH acts as the reducing cofactor, which can be regenerated by the crude enzyme system itself using glucose as an auxiliary substrate.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Xie Y,Xu JH,Lu WY,Lin GQdoi
10.1016/j.biortech.2008.11.054subject
Has Abstractpub_date
2009-05-01 00:00:00pages
2463-8issue
9eissn
0960-8524issn
1873-2976pii
S0960-8524(08)01066-3journal_volume
100pub_type
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