Adzuki bean: a new resource of biocatalyst for asymmetric reduction of aromatic ketones with high stereoselectivity and substrate tolerance.

Abstract:

:A new resource of biocatalyst for asymmetric reduction of aromatic ketones has been discovered for the first time from a common plant seed, adzuki bean, i.e. Phaseolus angularis (Willd.) W.F. Wight. The study investigated the best methods to prepare the biocatalyst and its ability to reduce ketones. Our results indicated that the biocatalyst from adzuki bean could reduce various aromatic ketones at relatively high concentrations (e.g. 100mM), exhibiting excellent stereoselectivity (>98% e.e.). In addition, it was found that NADPH acts as the reducing cofactor, which can be regenerated by the crude enzyme system itself using glucose as an auxiliary substrate.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Xie Y,Xu JH,Lu WY,Lin GQ

doi

10.1016/j.biortech.2008.11.054

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

2463-8

issue

9

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(08)01066-3

journal_volume

100

pub_type

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