Abstract：
:One of the goals of metabolic engineering is to engineer strains that can optimally produce target metabolites. However, the current workflow for rational engineering of the metabolic pathway is sometimes time-consuming and labor-intensive. Here, we have established a cost-effective approach for screening for variants secreting metabolites. Different surface display systems were adopted and verified, which anchored pHluorin to the Candida glabrata cell surface to associate pyruvic acid detection with the read out of this reporter. A generalizable simulation approach based on computational fluid dynamics and regularity of generated droplet dimension was presented, which was found to be an efficient design tool to explore microfluidic characteristics or optimization. Finally, a microfluidic platform based on simulation coupled with surface display system was constructed. A mutant exhibiting a 73.6% increase in pyruvic acid production was identified. This ultrahigh-throughput screening pattern offers a practical guide for identifying microbial strains with many traits of interest.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Guo L,Zeng W,Xu S,Zhou Jdoi
10.1016/j.biortech.2020.124258subject
Has Abstractpub_date
2020-12-01 00:00:00pages
124258eissn
0960-8524issn
1873-2976pii
S0960-8524(20)31532-7journal_volume
318pub_type
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