Abstract:
:Sperm antigens are being sought for the development of prototype contraceptive vaccines. For vaccine-induced antisperm antibodies to inhibit the function of viable sperm, target antigens must be expressed on the sperm surface before, during or after the acrosome reaction. The purpose of this study was to develop an approach to detect accurately surface binding of antibodies to viable acrosome-intact or acrosome-reacted sperm, and to confirm sperm surface binding characteristics of a panel of antisperm monoclonal antibodies submitted to the World Health Organization Antifertility Vaccines Task Force. Monoclonal antibodies were tested using a combination of carboxyl-fluorescein diacetate (CoFDA) sperm viability, and rhodamine immunofluorescence assays to ascertain surface binding to viable membrane-intact human sperm, and, in parallel studies, with a combination of fluorescein isothiocyanate (FITC) conjugated Pisum sativum agglutinin (PSA) and rhodamine immunofluorescence assays to ascertain the acrosomal status of antibody-positive sperm. Only 14 out of 32 antibodies that were previously considered to be surface-reactive were confirmed to be positive with these methods. Five were positive on unfixed viable (CoFDA-labelled) fresh sperm in this study, and 9 different antibodies were positive on unfixed acrosome-reacted sperm. We conclude that this multifaceted approach is more accurate and informative than previous techniques used for detection of sperm surface antigens such as radioimmunoassay, standard immunofluorescence, sperm agglutination assays and enzyme-linked immunosorbent assays, which can give false positive results due to the presence of contaminating non-sperm cells, insoluble seminal plasma material, or permeabilized non-viable sperm. Furthermore, the combination of assays employed in this study does not depend on sperm motility, as do the immunobead and sperm immobilization assays, and provides information about the acrosomal status of antibody-positive cells.
journal_name
J Reprod Immunoljournal_title
Journal of reproductive immunologyauthors
Fichorova R,Anderson DJdoi
10.1016/0165-0378(91)90019-msubject
Has Abstractpub_date
1991-05-01 00:00:00pages
1-13issue
1eissn
0165-0378issn
1872-7603pii
0165-0378(91)90019-Mjournal_volume
20pub_type
杂志文章abstract::This study has assessed the immunological and biological efficacy of two different doses of a recombinant GnRH vaccine administered to intact domestic cats. Fifteen kittens, 8-9 weeks of age, were allocated to three unequal treatment groups: group 1, 1 male control cat; group 2, 5 females administered 10 microg of GnR...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2004.08.004
更新日期:2004-12-01 00:00:00
abstract::Human pre-implantation stage embryos cultured in vitro spontaneously secreted a factor capable of inducing histamine-release from human blood basophils. The embryo-derived histamine-releasing factor (EHRF) has been isolated from the culture medium by means of heparin-Sepharose affinity chromatography. The factor bound...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(88)90047-2
更新日期:1988-06-01 00:00:00
abstract::The sequence of biochemical and molecular events associated with decidualization in the primate remain unclear. In the baboon, the sequential changes during this period in vivo are characterized by the downregulation of alpha-smooth actin followed by induction of cyclooxygenase-2 (COX-2) at the implantation site and t...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/s0165-0378(01)00141-3
更新日期:2002-05-01 00:00:00
abstract::This study examines the distribution of two carbohydrate determinants recognized by monoclonal antibodies EMA-1 and EMA-6 on cells in culture, adult mouse tissues and day 0-7 embryos, and provides partial biochemical characterization of the molecules carrying the determinants for EMA-1 on embryonal carcinoma cells. Bo...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(87)90069-6
更新日期:1987-02-01 00:00:00
abstract::No medical intervention guideline for prenatally diagnosed symptomatic congenital cytomegalovirus infection (CCMVI) is currently available. The aim of the study was to assess the efficacy of immunoglobulin fetal therapy for symptomatic CCMVI. With informed consent, hyper-immunoglobulin was injected into the peritoneal...
journal_title:Journal of reproductive immunology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.jri.2012.05.002
更新日期:2012-09-01 00:00:00
abstract::Progesterone and glucocorticoids share important anti-inflammatory and immunosuppressive properties. Both hormones have potent anti-proliferative effects in MLR, mitogen activation and cytotoxic T-cell generation. We investigated the cellular target of this in vitro anti-proliferative activity by comparing the effects...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(83)90237-1
更新日期:1983-07-01 00:00:00
abstract::For some time, reproductive immunologists have worked to understand the balance between maternal tolerance of the fetus, maternal health, and fetal protection which leads to successful pregnancy in mammalian species. We have always understood the potential importance of multiple factors, including nutrition, genetics,...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2017.09.005
更新日期:2017-09-01 00:00:00
abstract::The process of ovarian follicular development is a long and arduous one, marked by dramatic proliferation and differentiation of both the somatic and germ cell elements. To a large extent, this explosive agenda is under the control of conventional endocrine principles, involving hormones such as pituitary gonadotropin...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/s0165-0378(98)00026-6
更新日期:1998-08-01 00:00:00
abstract::Although antisperm autoantibody responses to obstruction of the male reproductive system have been documented, information on the nature of the cognate sperm autoantigens has been limited. In the present study, the patterns of sperm autoantigens recognized by sera from rats after obstruction of the vas deferens or epi...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(98)00090-4
更新日期:1999-05-01 00:00:00
abstract::Alloreactive T levels of para-aortic lymph nodes (PALN) and spleen were determined on different days of pregnancy in BALB/c females by local and systemic graft-versus-host (GvH) assays. A significant increase in GvH reactivity was registered early in both allogeneic and syngeneic matings, operating not only towards pa...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(85)90036-1
更新日期:1985-11-01 00:00:00
abstract::The numbers of intra-epithelial lymphocytes per 5 mm of uterine epithelium were counted in cyclic (n = 18) and pregnant (n = 18) gilts on days 10, 13, 16 and 19 postestrus. In cyclic animals these cells increased (P less than 0.01) from 26.4 +/- 1.1 per 5 mm at the mid-luteal phase (day 10) to 47.7 +/- 1.5 per 5 mm at...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(88)90034-4
更新日期:1988-10-01 00:00:00
abstract::Controversy exists as to whether active immunotherapy with allogeneic lymphocyte transfusions (ALT) or passive immunotherapy with intravenous immunoglobulin (IvIg) improve the chance of live birth in women with unexplained recurrent miscarriages (RM). Meta-analyses of the placebo-controlled trials carried out as Cochr...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/j.jri.2003.09.003
更新日期:2004-06-01 00:00:00
abstract::Endometriosis is a prevalent gynaecological disorder with a still unclear pathogenesis. So far inflammatory mechanisms are associated with disease progression and critical reviews have discussed the so-called 'danger theory' related to endometriosis. Hence, we performed immunoassays to evaluate whether local inflammat...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2018.07.006
更新日期:2018-11-01 00:00:00
abstract::Secondary recurrent miscarriage (RM) is defined as three or more consecutive pregnancy losses after delivery of a viable infant. Previous reports suggest that a firstborn male child is associated with less favourable subsequent reproductive potential, possibly due to maternal immunisation against male-specific minor h...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2010.10.004
更新日期:2011-01-01 00:00:00
abstract::This study showed that there are relationships between thymic and adrenal/ovarian endocrine actions. A 4 mg/kg dose of thymosin fraction 5 (TSN5) advanced vaginal opening and elevated estrogen levels. Single estradiol benzoate (EB) injections decreased thymus weight and caused a transient reduction in circulating plas...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(84)90039-1
更新日期:1984-01-01 00:00:00
abstract::Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a classical haematopoietic cytokine which has also been implicated in placental growth and development. In this study we have performed a detailed immunohistological localization of the low affinity GM-CSF receptor (GM-CSF-R alpha) in human first trimester i...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(94)90037-x
更新日期:1994-03-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to investigate the sex specific expression of histone protein modifications responsible for rapid gene expression in IUGR placentas. PATIENTS AND METHODS:We screened for fetal sex-specific expression of the histone proteins H3K4me3 and H3K9ac in 23 IUGR and 40 control placentas...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2020.103124
更新日期:2020-06-01 00:00:00
abstract::Although inflammation is associated with risk of gestational diabetes mellitus (GDM), little is known if there is an association between inflammation and GDM in African-American women, a group at higher risk for GDM complications. In the present study, we aimed to determine if selected inflammatory cytokines (i.e. TNF...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2016.03.005
更新日期:2016-06-01 00:00:00
abstract::Hypertensive disorders of pregnancy (HDP) represent 10% of human births globally and the major complication preeclampsia has a 3-5% prevalence. The etiology of HDP remains uncertain; however, major advances have been made over the last 25 years. The Seventh International Workshop on Reproductive Immunology, Immunologi...
journal_title:Journal of reproductive immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.jri.2011.02.003
更新日期:2011-05-01 00:00:00
abstract::Cytokines present in the reproductive system play an important role both in the modulation of immune responses to infectious challenge and in the establishment and maintenance of pregnancy. Interleukin 18 (IL-18) has been regarded as an important regulator of innate and acquired immune response, but its expression and...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2011.07.002
更新日期:2011-12-01 00:00:00
abstract::In a prospective study 140 couples who had at least three spontaneous abortions (RSA) were studied for the presence of Fc receptor blocking antibodies detected by the erythrocyte antibody rosette inhibition (EAI) assay, for anti-paternal cytotoxic antibodies (APCA), and for mixed lymphocyte culture inhibiting (MLCI) a...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(91)90030-t
更新日期:1991-07-01 00:00:00
abstract::Herpes simplex virus type 2 (HSV-2) is becoming increasingly prevalent worldwide, despite the widespread use of antiviral drugs. Its ability to evade the immune system and establish a latent infection has made it difficult to develop an effective vaccine. Our understanding of the immune response against HSV-2 remains ...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/j.jri.2011.01.001
更新日期:2011-03-01 00:00:00
abstract::Currently, different approaches are used to select oocytes for in vitro fertilization (IVF) procedures, but they do not assure a significant association with the pregnancy outcome. Since several studies have proposed the expression of HLA-G antigens in early embryos to be a possible marker of elevated implantation rat...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2007.02.005
更新日期:2007-06-01 00:00:00
abstract::Lipopolysaccharide (LPS, endotoxin) is a component of the cell wall of gram-negative bacteria and a potent inducer of severe inflammatory reactions. In mice, systemically administered LPS induces fetal resorption and increases fetal mortality. However, effects of intrauterine LPS on fertility, fetal survival and devel...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(98)00078-3
更新日期:1999-01-01 00:00:00
abstract::Development of a vaccine(s) based on sperm antigens represents a promising approach to contraception. The utility of an antigen in immunocontraception is contingent upon sperm/testis-specificity and its involvement in the fertilization process. Since, the sperm-zona pellucida (ZP) recognition and binding constitutes t...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(01)00094-8
更新日期:2002-01-01 00:00:00
abstract::In addition to natural killer cells, other innate lymphoid cells have recently been identified in the mouse and human uterus, but their roles in successful pregnancy remain poorly defined. In this study, we examined the dynamic changes of uterine innate lymphoid cells throughout pregnancy in mice. We found that the to...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2017.09.003
更新日期:2017-09-01 00:00:00
abstract::The isolation and partial characterization of an immunosuppressive early pregnancy factor (EPF) present in the serum of pregnant women, between 3 and 8 weeks of gestation, is described. EPF was purified using ion-exchange chromatography, affinity chromatography and HPLC gel permeation techniques. A homogeneous, active...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(86)90018-5
更新日期:1986-11-01 00:00:00
abstract::Few studies have assessed longitudinal changes in circulating cytokine levels during normal pregnancy. We have examined the natural history of maternal plasma cytokines from early- to mid-pregnancy in a large, longitudinal cohort. Multiplex flow cytometry was used to measure interleukin (IL)-2, IL-6, IL-12, tumor necr...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2007.06.051
更新日期:2008-04-01 00:00:00
abstract::Purified midgestation murine trophoblast cannot be killed by a variety of cell-mediated effector mechanisms, with the exception of highly lytic effectors such as lymphokine-activated killer cells. We now report that this trophoblast population is also resistant to tumor necrosis factor (TNF)-alpha. ...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(90)90043-6
更新日期:1990-03-01 00:00:00
abstract::Despite immense effort, the development of vaccines effective at mucosal sites has proceeded at a faltering pace. Efforts concentrating on humoral immunity but neglecting cellular immunity may be misdirected by ignoring many viral mucosal pathogens. Improved understanding of the development and maintenance of lymphocy...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(01)00110-3
更新日期:2001-10-01 00:00:00