Thyroxine and 3,3',5-triiodothyronine are glucuronidated in rat liver by different uridine diphosphate-glucuronyltransferases.


:Male Wistar rats were treated with 50 mg 3,3',4,4'-tetrachlorobiphenyl (TCB)/kg BW or vehicle. After 4 days, the livers were isolated and perfused for 90 min with 2 nM [125I]T3 or 10 nM [125I]T4 in Krebs-Ringer medium containing 1% albumin. Deiodination and conjugation products and remaining substrates were determined in bile and medium samples by Sephadex LH-20 chromatography and HPLC. TCB treatment did not affect hepatic uptake and metabolism of T3. However, biliary excretion of T4 glucuronide was strongly increased by TCB, resulting in an augmented T4 disappearance from the medium, although initial hepatic uptake of T4 was not altered. Measurement of the microsomal UDP-glucuronyltransferase (UDPGT) activities confirmed that T4 UDPGT was induced by TCB, whereas T3 glucuronidation was unaffected. T3 UDPGT activity showed a discontinuous variation, which completely matched the genetic heterogeneity in androsterone glucuronidation in Wistar rats. These results indicate that different isozymes catalyze the glucuronidation of T3 and T4.






Beetstra JB,van Engelen JG,Karels P,van der Hoek HJ,de Jong M,Docter R,Krenning EP,Hennemann G,Brouwer A,Visser TJ




Has Abstract


1991-02-01 00:00:00












  • The direct in vitro effect of insulin-like growth factors (IGFs) on normal bovine mammary cell proliferation and production of IGF binding proteins.

    abstract::Mammary epithelial cells isolated from pregnant, nonlactating heifers were grown in vitro using collagen substrates. Using these systems, the truncated form of insulin growth factor-1 (IGF-1) (des-3-IGF-1), IGF-1, and IGF-2 all stimulated a significant (0.5 to 1 fold) increase in cell proliferation (des-3-IGF-1 greate...


    pub_type: 杂志文章


    authors: McGrath MF,Collier RJ,Clemmons DR,Busby WH,Sweeny CA,Krivi GG

    更新日期:1991-08-01 00:00:00

  • Wortmannin-sensitive and -insensitive steps in calcium-controlled exocytosis in pituitary gonadotrophs: evidence that myosin light chain kinase mediates calcium-dependent and wortmannin-sensitive gonadotropin secretion.

    abstract::In cultured rat pituitary cells, increases in the cytosolic calcium concentration ([Ca2+]i) and LH release are induced by activation of GnRH receptors as well as by nonreceptor-mediated stimuli. Treatment of pituitary cells with the myosin light chain kinase (MLCK) inhibitor, wortmannin, attenuated GnRH-induced LH rel...


    pub_type: 杂志文章


    authors: Rao K,Paik WY,Zheng L,Jobin RM,Tomić M,Jiang H,Nakanishi S,Stojilkovic SS

    更新日期:1997-04-01 00:00:00

  • Differential binding and neutralization of activins A and B by follistatin and follistatin like-3 (FSTL-3/FSRP/FLRG).

    abstract::Modulation of activin and other TGF beta superfamily signaling is the primary mechanism of action for both follistatin (FS) and FS-like 3 (FSTL-3). However, most studies of these ligands use activin A due to its wide availability. We have now tested the ability of FS288 and FSTL-3 to bind and neutralize activin B rela...


    pub_type: 杂志文章


    authors: Schneyer A,Schoen A,Quigg A,Sidis Y

    更新日期:2003-05-01 00:00:00

  • The clearance of ACTH from the plasma of adult and fetal sheep.

    abstract::The disappearance of 125-I-ACTH from the circulation of 4 pregnant and fetal sheep has been followed after a single injection to the ewe or fetus. The mean metabolic clearance rate for the fetus and the ewe was 55 and 34 ml/min/kg respectively, giving a half-life in each case of about 1 min. The higher fetal than mate...


    pub_type: 杂志文章


    authors: Jones CT,Luther E,Ritchie JW,Worthington D

    更新日期:1975-01-01 00:00:00

  • WNT4 acts downstream of BMP2 and functions via β-catenin signaling pathway to regulate human endometrial stromal cell differentiation.

    abstract::Differentiation of endometrial stromal cells into decidual cells is a prerequisite for successful embryo implantation. Our previous studies in the mouse have shown that bone morphogenetic protein 2 (BMP2), a morphogen belonging to the TGFβ superfamily, is essential for this differentiation process. BMP2 is markedly in...


    pub_type: 杂志文章


    authors: Li Q,Kannan A,Das A,Demayo FJ,Hornsby PJ,Young SL,Taylor RN,Bagchi MK,Bagchi IC

    更新日期:2013-01-01 00:00:00

  • Changes in the kinase activity of the insulin receptor account for an increased insulin sensitivity of mammary gland in late pregnancy.

    abstract::Mammary gland is an organ that undergoes cycles of growth, differentiation, and function during pregnancy and lactation. Although it is known that the gland enhances its sensitivity to insulin during lactation, it remains to be investigated whether this increased sensitivity develops during pregnancy and which are the...


    pub_type: 杂志文章


    authors: Carrascosa JM,Ramos P,Molero JC,Herrera E

    更新日期:1998-02-01 00:00:00

  • Intracerebroventricular Catalase Reduces Hepatic Insulin Sensitivity and Increases Responses to Hypoglycemia in Rats.

    abstract::Specialized metabolic sensors in the hypothalamus regulate blood glucose levels by influencing hepatic glucose output and hypoglycemic counterregulatory responses. Hypothalamic reactive oxygen species (ROS) may act as a metabolic signal-mediating responses to changes in glucose, other substrates and hormones. The role...


    pub_type: 杂志文章


    authors: Pauliina Markkula S,Lyons D,Yueh CY,Riches C,Hurst P,Fielding B,Heisler LK,Evans ML

    更新日期:2016-12-01 00:00:00

  • Basic fibroblast growth factor induces proteolysis of secreted and cell membrane-associated insulin-like growth factor binding protein-2 in human neuroblastoma cells.

    abstract::Insulin-like growth factor (IGF) action in the brain is modulated by IGF-binding proteins (IGFBPs) whose abundance can be altered by other locally expressed growth factors. However, the mechanisms involved are unclear. We here employed the neuroblastoma cell line SK-N-MC as a model to define the mechanisms involved in...


    pub_type: 杂志文章


    authors: Russo VC,Rekaris G,Baker NL,Bach LA,Werther GA

    更新日期:1999-07-01 00:00:00

  • Nitrogenous compounds reversibly inhibit the adenosine 3',5'-monophosphate response to thyrotropin: this effect is dissociated from altered guanine 3',5'-monophosphate levels.

    abstract::Nitrogenous compounds were studied for their effect on cGMP levels in the thyroid. Several agents, including sodium nitrite and hydroxylamine, could generate a rise of over 100-fold in cGMP. At a concentration of 20 mM, HN2OH and NaNO2 significantly reduced cAMP levels previously generated by 0.5 mU/ml TSH. If the sli...


    pub_type: 杂志文章


    authors: Spaulding SW

    更新日期:1979-09-01 00:00:00

  • Effects of vitamin D3 deficiency on estradiol-17 beta metabolism in the laying hen.

    abstract::Evidence is presented that in vitamin D deficiency there are aberrations of estrogen metabolism in the laying hen. Five White Leghorn hens, 35 weeks of age, all laying soft shelled eggs as a result of complete withdrawal of supplemental vitamin D3 (D3) from the diet, were found to have a 3-fold higher molar ratio of p...


    pub_type: 杂志文章


    authors: Tsang CP,Grunder AA

    更新日期:1984-12-01 00:00:00

  • Expression of calbindin-D decreases with age in intestine and kidney.

    abstract::The calbindins are Ca-binding proteins whose expression is regulated by 1,25-dihydroxyvitamin D3, the active metabolite of vitamin D3. The calbindins are found in high amounts in the proximal intestine (calbindin-D-9k) and the kidney (calbindin-D-28k), and they are thought to play a role in Ca transport by these tissu...


    pub_type: 杂志文章


    authors: Armbrecht HJ,Boltz M,Strong R,Richardson A,Bruns ME,Christakos S

    更新日期:1989-12-01 00:00:00

  • Comparison of the signaling abilities of the cytoplasmic domains of the insulin receptor and the insulin receptor-related receptor in 3T3-L1 adipocytes.

    abstract::In the present work a chimeric receptor containing the intracellular domain of the insulin receptor-related receptor (IRR) and the extracellular domain of the colony stimulating factor-1 (CSF-1) receptor was expressed in 3T3-L1 adipocytes and compared with the parallel chimeric receptor containing the cytoplasmic doma...


    pub_type: 杂志文章


    authors: Dandekar AA,Wallach BJ,Barthel A,Roth RA

    更新日期:1998-08-01 00:00:00

  • Requirement for IGF-I in epidermal growth factor-mediated cell cycle progression of mammary epithelial cells.

    abstract::Induction of cyclin proteins is required for progression of cells through the G(1)-S and G(2)-M cell cycle checkpoints and is a primary mechanism by which mitogens regulate cell cycle progression. IGF-I and the epidermal growth factor (EGF)-related ligands are mitogens for mammary epithelial cells in vitro and are ess...


    pub_type: 杂志文章


    authors: Stull MA,Richert MM,Loladze AV,Wood TL

    更新日期:2002-05-01 00:00:00

  • Insulin-like growth factor I gene expression by primary cultures of ovarian cells: insulin and dexamethasone dependence.

    abstract::A growing body of information now supports the existence of a complete intraovarian insulin-like growth Factor I (IGF-I) system replete with ligands, receptors, and binding protein(s). However, studies concerned with the regulation of ovarian IGF-I gene expression remain scarce. It was thus the objective of this commu...


    pub_type: 杂志文章


    authors: Botero LF,Roberts CT Jr,LeRoith D,Adashi EY,Hernandez ER

    更新日期:1993-06-01 00:00:00

  • Deoxyribonucleic acid synthesis-dependent casein gene expression: species differences.

    abstract::Mammary gland explants from mature virgin mice, rats, and rabbits exhibit an increased rate of both DNA and casein syntheses when cultured in the presence of specific combinations of insulin, hydrocortisone, and PRL. If cytosine arabinoside, a potent inhibitor of DNA synthesis, is added to the culture medium, casein s...


    pub_type: 杂志文章


    authors: Nicholas KR,Bolander FF Jr,Sankaran L,Topper YJ

    更新日期:1983-03-01 00:00:00

  • Tamoxifen inhibits cell proliferation via mitogen-activated protein kinase cascades in human ovarian cancer cell lines in a manner not dependent on the expression of estrogen receptor or the sensitivity to cisplatin.

    abstract::Although tamoxifen (TAM), which is widely used in the treatment of breast cancer, also has a beneficial effect on cisplatin-refractory ovarian cancer, the biological mechanism of this effect has remained obscure. TAM, besides its action as an antiestrogen, also inhibits cell proliferation of estrogen receptor (ER)-neg...


    pub_type: 杂志文章


    authors: Mabuchi S,Ohmichi M,Kimura A,Ikebuchi Y,Hisamoto K,Arimoto-Ishida E,Nishio Y,Takahashi K,Tasaka K,Murata Y

    更新日期:2004-03-01 00:00:00

  • Binding characteristics of a biologically active variant of human growth hormone (20K) to growth hormone and lactogen receptors.

    abstract::The dose-dependent displacement characteristics of a biologically active 20,000 molecular weight human pituitary growth hormone variant (20K) and of human growth hormone (hGH) were compared using hGH liver plasma membrane receptors both from 20-30 day pregnant rabbits and from normal female rats and also using mammary...


    pub_type: 杂志文章


    authors: Sigel MB,Thorpe NA,Kobrin MS,Lewis UJ,Vanderlaan WP

    更新日期:1981-04-01 00:00:00

  • Prostaglandin F2 alpha stimulates inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate formation in bovine luteal cells.

    abstract::The present studies were conducted to further evaluate inositol phosphate formation and metabolism in prostaglandin F2 alpha (PGF2 alpha)-stimulated bovine luteal cells. Corpora lutea were dispersed with collagenase, and luteal cells were prelabeled for 3 h with [3H]inositol. Inositol phosphates produced in response t...


    pub_type: 杂志文章


    authors: Duncan RA,Davis JS

    更新日期:1991-03-01 00:00:00

  • Metabolic clearance of endogenous and radioiodinated thyroglobulin in rats.

    abstract::The basal concentration and metabolic clearance of radiolabeled and endogenous thyroglobulin (Tg) were measured in normal male rats. Serum Tg concentrations (by RIA) in Sprague-Dawley adult males ranged from 70-680 ng/ml (mean +/- SD, 218 +/- 177). In male Fisher rats, basal serum Tg concentrations were 32-263 ng/ml. ...


    pub_type: 杂志文章


    authors: Izumi M,Larsen PR

    更新日期:1978-07-01 00:00:00

  • Anterior pituitary vasodilation after hemorrhage in the dog.

    abstract::A miniature thermoelectric probe was used to record continuously tissue blood flow in the anterior pituitary gland of anesthetized dogs. The output of the flow probe is linear in the range of tissue blood flow encountered in the pituitary. Probe placement required minimal tissue trauma, and zero blood flow was recorde...


    pub_type: 杂志文章


    authors: Kopaniky DR,Gann DS

    更新日期:1975-09-01 00:00:00

  • A reexamination of the proposed inactivation of thyroid peroxidase in the rat thyroid by propylthiouracil.

    abstract::The antithyroid drug 6-propylthiouracil (PTU) was previously shown in our laboratory to have an unexpectedly prolonged inhibitory effect on iodination in the thyroid glands of rats. Eighteen hours after injection of a relatively small dose, iodination in the thyroid remained inhibited by more than 90%. We previously s...


    pub_type: 杂志文章


    authors: Taurog A,Dorris ML

    更新日期:1989-06-01 00:00:00

  • Estrogen stimulation of cell migration involves multiple signaling pathway interactions.

    abstract::Hormone-dependent breast cancers respond to inhibitors of estrogen synthesis or action with tumor regression and with a reduction of new metastases. The mechanisms underlying the effects of estrogen on metastasis likely differ from those on tumor regression. Cell migration is a key first step in the metastatic process...


    pub_type: 杂志文章


    authors: Li Y,Wang JP,Santen RJ,Kim TH,Park H,Fan P,Yue W

    更新日期:2010-11-01 00:00:00

  • Unique properties of the follicle-stimulating hormone- and cholera toxin-sensitive adenylyl cyclase of immature granulosa cells.

    abstract::Previous studies have shown that the adenylyl cyclase of intact granulosa cells from immature porcine follicles has a uniquely prolonged responsiveness to FSH and an atypically poor responsiveness to cholera toxin relative to other cells. The present studies were designed to determine if these characteristics were due...


    pub_type: 杂志文章


    authors: Hunzicker-Dunn M,LaBarbera AR

    更新日期:1986-01-01 00:00:00

  • Effects of N-methyl-D,L-aspartic acid on luteinizing hormone secretion in normal mice and in hypogonadal mice with fetal preoptic area implants.

    abstract::Many aspects of reproductive function are corrected in hypogonadal mice with preoptic area grafts (HPG/POA). Gonadotropin release and gonadal development are dependent on the presence of GnRH cells within the grafts and GnRH innervation of the median eminence. This study examined the effect of a known modulator of GnR...


    pub_type: 杂志文章


    authors: Saitoh Y,Silverman AJ,Gibson MJ

    更新日期:1991-05-01 00:00:00

  • Role of central nervous system-derived or circulating gamma-aminobutyric acid on prolactin secretion in the rat.

    abstract::To investigate the respective role in PRL secretion of gamma-aminobutyric acid (GABA), either derived from the central nervous system or circulating in plasma, experiments were performed using ethanolamine-O-sulfate (EOS), a specific inhibitor of GABA catabolism. Intracerebroventricular injection of EOS (2 mg/kg) indu...


    pub_type: 杂志文章


    authors: Apud JA,Racagni G,Iuliano E,Cocchi D,Casanueva F,Müller EE

    更新日期:1981-04-01 00:00:00

  • Secretory protein 7B2 is associated with pancreatic hormones within normal islets and some experimentally induced tumors.

    abstract::7B2 is a novel neuroendocrine polypeptide which belongs to an entirely new superfamily of proteins. In extension of previous reports on 7B2, these studies concern its expression in endocrine pancreatic tissue. They have been performed using specific antibodies prepared against two distinct synthetic fragments of 7B2 c...


    pub_type: 杂志文章


    authors: Benjannet S,Marcinkiewicz M,Falgueyret JP,Johnson DE,Seidah NG,Chrétien M

    更新日期:1988-08-01 00:00:00

  • Arcuate nucleus-specific leptin receptor gene therapy attenuates the obesity phenotype of Koletsky (fa(k)/fa(k)) rats.

    abstract::Leptin signaling in the hypothalamic arcuate nucleus (ARC) is hypothesized to play an important role in energy homeostasis. To investigate whether leptin signaling limited to this brain area is sufficient to reduce food intake and body weight, we used adenoviral gene therapy to express the signaling isoform of the lep...


    pub_type: 杂志文章


    authors: Morton GJ,Niswender KD,Rhodes CJ,Myers MG Jr,Blevins JE,Baskin DG,Schwartz MW

    更新日期:2003-05-01 00:00:00

  • Ontogeny and effect of vitamin D deprivation on rat serum 25-hydroxyvitamin D binding protein.

    abstract::Specific serum binding of 25-hydroxy-cholecalciferol (25-OHD3) was measured by saturation analysis in rats of various ages, and during vitamin D deprivation. The serum binding capacity for 25-OHD3 was observed to increase until age 6-8 weeks, then decline and remain stable thereafter at 3.7 x 10(-6) M. The 25-hydroxyv...


    pub_type: 杂志文章


    authors: Rojanasathit S,Haddad JG

    更新日期:1977-03-01 00:00:00

  • Epidermal growth factor family members: endogenous mediators of the ovulatory response.

    abstract::Previous studies showed that epidermal growth factor (EGF) and TGFalpha mimic the action of LH on the resumption of oocyte maturation. We tested whether EGF-like agents, such as amphiregulin (AR), epiregulin (ER), and betacellulin (BTC), also mediate the LH stimulation of the ovulatory response in the rat. LH induced ...


    pub_type: 杂志文章


    authors: Ashkenazi H,Cao X,Motola S,Popliker M,Conti M,Tsafriri A

    更新日期:2005-01-01 00:00:00

  • Localization of messenger ribonucleic acids for insulin-like growth factor I (IGF-I), IGF-II, and the type 1 IGF receptor in the ovine ovary throughout the estrous cycle.

    abstract::The distribution pattern of messenger RNAs (mRNAs) for insulin-like growth factor I (IGF-I), IGF-II, and the type 1 IGF receptor and the detection of IGF-binding sites in sections of ovine ovary were demonstrated using in situ hybridization and autoradiography. Ovaries were collected from 30 ewes at time points throug...


    pub_type: 杂志文章


    authors: Perks CM,Denning-Kendall PA,Gilmour RS,Wathes DC

    更新日期:1995-12-01 00:00:00