Abstract:
:Secretory leukocyte protease inhibitor (SLPI) is an endogenous serine protease inhibitor that protects the lungs from excessive tissue damage caused by leukocyte proteases released during inflammation. Recombinant SLPI (rSLPI) has shown potential as a treatment for inflammatory lung conditions. To date, its clinical application has been limited by rapid enzymatic cleavage by cathepsins and rapid clearance from the lungs after inhalation. In this study, rSLPI was encapsulated in 1,2-Dioleoyl-sn-Glycero-3-[Phospho-L-Serine] : Cholesterol (DOPS : Chol) liposomes for inhalation. Incubation of rSLPI with cathepsin L leads to complete loss of activity while encapsulation of rSLPI in DOPS : Chol liposomes retained 92.6% of its activity after challenge with cathepsin L. rSLPI-loaded liposomes were aerosolized efficiently using a standard nebulizer with a minimal loss of activity and stability. This formulation was biocompatible and encapsulation did not appear to diminish access to intracellular sites of action in in vitro cell culture studies. Liposome encapsulation of rSLPI therefore improves stability and potentially reduces the level and frequency of dosing required for therapeutic effect after inhalation.
journal_name
J Microencapsuljournal_title
Journal of microencapsulationauthors
Gibbons AM,McElvaney NG,Taggart CC,Cryan SAdoi
10.1080/02652040802466535subject
Has Abstractpub_date
2009-09-01 00:00:00pages
513-22issue
6eissn
0265-2048issn
1464-5246pii
904257611journal_volume
26pub_type
杂志文章abstract::The purpose of this research work was to formulate and systematically evaluate in vitro performance of mucoadhesive microspheres of lacidipine for treatment of pylorospasm. Lacidipine microspheres containing chitosan were prepared by chemical denaturation using glutaraldehyde as a cross-linking agent. The microspheres...
journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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更新日期:2014-01-01 00:00:00
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journal_title:Journal of microencapsulation
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abstract::A combined approach based on drug cyclodextrin (CD) complexation and loading into PLGA nanoparticles (NP) has been developed to improve oxaprozin therapeutic efficiency. This strategy exploits the solubilizing and stabilizing properties of CDs and the prolonged-release and targeting properties of PLGA NPs. Drug-loaded...
journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
pub_type: 杂志文章,评审
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journal_title:Journal of microencapsulation
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abstract::Nanoparticles prepared of human serum albumin (HSA) represent promising carriers for drug delivery. Desolvating procedure is a common method to obtain protein-based particles. However process parameters have a great influence on size, size distribution and particle yield of the resulting formulation. Therefore, it is ...
journal_title:Journal of microencapsulation
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更新日期:2012-01-01 00:00:00