Abstract:
AIM:To define whether human colonic mucosa exposure to Lactobacillus rhamnosus GG (LGG), American Type Culture Collection (ATCC) 53103, may influence intestinal muscle cell contractility. METHODS:Human colon specimens were obtained from disease-free margins of resected segments for cancer. The mucosa and submucosa, after dissection, were sealed between 2 chambers, with the luminal side of the mucosa facing upward and covered with 5 mL of Krebs solution and the submucosal side facing downward into 20 mL of Krebs solution. LGG or normal undernatant (N-undernatant) were added to the luminal side of the mucosa for 30 minutes. Smooth muscle cells (SMCs), isolated from the circular muscle layer, were exposed to undernatant for 30 minutes from the submucosal chamber of mucosa that was either preexposed to N-undernatant or to LGG (36 x 10(-9) colony forming units/mL) (LGG-undernatant). Acetylcholine (Ach) dose-response was obtained for SMCs. RESULTS:SMCs exposed to N-undernatant presented a dose-response to Ach (maximal contraction: 32%+/-5% with 1-muM Ach) that is similar to unstimulated SMCs. Exposure to LGG-undernatant resulted both in an 18%+/-3% cell shortening and a 78%+/-7% inhibition of maximal Ach-induced contraction. When SMCs were directly exposed to LGG, a significant impairment of contraction (70%+/-5%, compared with control cells) and a dose-dependent and time-dependent shortening were observed. CONCLUSIONS:After acute exposure of colonic mucosa to LGG, a significant shortening of SMCs is observed that possibly contributes to the reduced contractile response to Ach. Further studies are needed to establish the mechanisms of this effect that could account for the clinical efficacy of probiotics in intestinal disorders.
journal_name
J Clin Gastroenteroljournal_title
Journal of clinical gastroenterologyauthors
Guarino MP,Altomare A,Stasi E,Marignani M,Severi C,Alloni R,Dicuonzo G,Morelli L,Coppola R,Cicala Mdoi
10.1097/MCG.0b013e31817e1cacsubject
Has Abstractpub_date
2008-09-01 00:00:00pages
S185-90eissn
0192-0790issn
1539-2031journal_volume
42 Suppl 3 Pt 2pub_type
杂志文章abstract::Few alternative treatments are available for those patients with autoimmune chronic active hepatitis who fail to respond to the conventional treatment of corticosteroids. Such patients have a poor prognosis and frequently require liver transplantation. We report a patient with autoimmune hepatitis who failed treatment...
journal_title:Journal of clinical gastroenterology
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更新日期:1993-12-01 00:00:00
abstract:GOALS:To compare the healthcare resource consumption of patients who have irritable bowel syndrome (IBS) with an age-, gender-, and comorbidity-matched cohort of patients without IBS from a managed care perspective. STUDY:Retrospective cohort analysis. Data were obtained electronically through the Henry Ford Health Sy...
journal_title:Journal of clinical gastroenterology
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abstract::The benefit of early endoscopy in the management of peptic ulcer bleeding remains controversial. In this study we looked at the role of early endoscopy in bleeding peptic ulcer patients with clear, "coffee grounds," or bloody nasogastric aspirate. A consecutive series of 325 patients with peptic ulcer bleeding were in...
journal_title:Journal of clinical gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Journal of clinical gastroenterology
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doi:10.1097/MCG.0000000000000184
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journal_title:Journal of clinical gastroenterology
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abstract::Two cases of Cruveilhier-Baumgarten syndrome not clinically evident and without esophageal varices in patients with liver cirrhosis and portal hypertension are presented. The diagnosis was made by real-time ultrasonography, which showed echographic caput medusae with large afferent umbilical veins and efferent inferio...
journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.1097/MCG.0b013e31802e70b1
更新日期:2008-03-01 00:00:00
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journal_title:Journal of clinical gastroenterology
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更新日期:2001-03-01 00:00:00
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journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
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abstract:INTRODUCTION:Cirrhosis is a major milestone in patients with chronic liver disease because of its impact on patient morbidity and mortality. Chronic hepatitis B (CHB) and hepatitis C (CHC) are important causes of cirrhosis. This systematic review examines the relevant literature and evidence to assess whether cirrhosis...
journal_title:Journal of clinical gastroenterology
pub_type: 杂志文章,评审
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journal_title:Journal of clinical gastroenterology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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更新日期:1991-01-01 00:00:00
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journal_title:Journal of clinical gastroenterology
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更新日期:2016-07-01 00:00:00
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journal_title:Journal of clinical gastroenterology
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journal_title:Journal of clinical gastroenterology
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